ANALYSIS OF GENETIC STABILITY IN THE ex vitro ROOTED MICROCUTTINGS OF BLUEBERRY (Vaccinium corymbosum L.)
Karolina Nowakowska
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, PolandAndrzej Pacholczak
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, PolandAbstract
During plant propagation changes in genetic stability may occur, not only during the in vitro multiplication but also in the ex vitro conditions in response to the sum of stress factors affecting plants. It is therefore necessary to evaluate planting material in terms of its genetic stability. The aim of the work was to assess by the RAPD method genetic stability of the ex vitro rooted blueberry (Vaccinium corymbosum ‘Bluecrop’ and ‘Duke’) microcuttings. Also an effect of biostimulator Goteo (0.2%) on rhizogenesis was tested as compared to the auxin indole-3-butyric acid IBA (50 mg·l–1) applied as water solution and a commercial rooting powder Rhizopon AA containing 1% IBA. All the rooting stimulators improved the percentage and degree of rooting in blueberry microcuttings. The biostimulator gave results only slightly poorer than the auxin. Both cultivars were genetically stable and no DNA polymorphism was found between the traditionally propagated stock plants growing in a nursery and those rooted in vivo, regardless of the rooting enhancer used.
Keywords:
ericaceous plants, RAPD, rhizogenesis, growth regulators, genetic stabilityReferences
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du Jardin, P. (2015). Plant biostimulants: definition, concept, main categories and regulation. Sci. Hortic., 196, 3–14.
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Hazarika, B.N. (2003). Acclimatization of tissue-cultured plants. J. Curr. Res. Sci., 85, 1704–1711.
Jain, S.M. (2001). Tissue culture-derived variation in crop improvement. Euphytica, 118, 153–166.
Khan, W., Rayirath, U.P., Subramanian, S., Jithesh, M.N., Rayorath, P., Hodges, D.M., Critchley, A.T., Craigie, J.S., Norrie, J., Prithiviraj, B. (2009). Seaweed extracts
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Kroin, J. (2010). Propagation of cuttings using foliar applied IBA in aqueous solutions at or after sticking. Comb. Proc. Int. Plant Propag. Soc., 60, 369–377.
Kroin, J. (2014). Development and application of foliar applied rooting solutions. Comb. Proc. Int. Plant Propag. Soc., 64, 431–436.
Kruk, K., Zapalska, M., Węgorek, T., Kowalczyk, K. (2012). Ocena zróżnicowania genetycznego dwóch populacji sosny zwyczajnej (Pinus sylvestris L.) z okolic Puław. Annales UMCS, sec. E, Agricultura, 67(2), 1–11.
Kumar, G., Sahoo, D. (2011). Effect of seaweed liquid extract on growth an yield of Triticum aestivum var. Pusa Gold. J. Appl. Phys., 23, 251–255.
Litwińczuk, W., Szczerba, G., Wrona, D. (2005). Field performance of highbush blueberries (Vaccinium corymbosum L.) cv. ‘Herbert’ propagated by cuttings
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Matysiak, K., Kaczmarek, S., Kierzek, R., Kardasz, P. (2010). Effect of seaweeds extracts and humic and fulvic acids on the germination and early growth of winter oilseed rape (Brassica napus L.). J. Res. Appl. Agric. Engineer., 55, 28–32.
Masari, M. (2009). Mass propagation of highbush blueberry using short cut dormant shoots. Jpn. Soc. Hortic. Sci., 8(2), 161–167.
Meiners, J., Schwab, M., Szankowski, I. (2007). Efficient in vitro regeneration for Vaccinium species. Plant Cell Tiss. Organ Cult., 89, 169–176.
Mugnai, S., Azzarello, E., Pandolfi, C., Salamagne, S., Briand, X., Mancuso, S. (2008). Enchancement of ammonium and potassium root influxes by the application
of marine bioactive substances positively affects Vitis vinifera plant growth. J. Appl. Phycol., 20, 177–182.
Ostrolucká, M.G., Gajdošová, A., Libiaková, G., Hrubíková, K., Bežo, M. (2007). Protocol for micropropagation of selected Vaccinium sp. Jain, S.M. Häggman, H. (eds). Protocols for Micropropagation of Woody Trees and Fruits. Springer, Berlin–Heidelberg–New York, 445–455.
Pacholczak, A., Nowakowska, K. (2015). The ex vitro rooting of blueberry (Vaccinium corymbosum L.) mictrocuttings. Folia Hortic., 27(2), 145–150.
Pacholczak, A., Szydło, W., Petelewicz, P., Szulczyk, K. (2013). The effect of Algamino Plant on rhizogenesis in stem cuttings of Physocarpus opulifolius ‘Dart’s Gold’ and ‘Red Baron’. Acta Sci. Pol. Hortorum Cultus, 12(3), 105–116.
Przybysz, A., Wrochna, M., Słowiński, A., Gawrońska, H. (2010). Stimulatory effect of Asahi SL on selected plant species. Acta Sci. Pol. Hortorum Cultus, 9(2), 53–63.
Read, P.E., Wildung, D.K., Hartley, C.A., Sandahal, J.G. (1989). Field performance of in vitro propagated ‘Northblue’ blueberries. Acta Hortic., 241, 191–194.
Rodriguez, J.M., Berke, T., Engle, L., Nienhuis, J. (1999). Variation among and within Capsicum species revealed by RAPD markers. Theor. Appl. Genet., 99, 147–156.
Ružic, D., Vujović, T., Libiakova, G., Cerović, R., Gajdošová, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). J. Berry Res., 2, 97–103.
Snedecor, G.W., Cochran, W.G. (1967). Statistical methods. The Iowa State University Press, Ames, Iowa, 593 pp.
Spethmann, W. (1998). Factor affecting rooting of difficult to root plants. Comb. Proc. Int. Plant Propag. Soc., 48, 200–205.
Strik, B. (2006). Blueberry production and research trends in North America. Acta Hortic., 715, 173–184.
Sunnocks, P. (2000). Efficient genetic markers for population biology. Trends Ecol. Evol., 15, 199–203.
Vescan, L.A., Pamfil, D., Clapa, D., Fira, A., Sisea, C.R., Pop, I.F., Petricele, I.V., Ciuzan, O., Pop, R. (2012). Efficient micropropagation protocol for highbush blueberry
(Vaccinium corymbosum L.) cv. ‘Elliot’. Rom. Biotech. Lett., 17(1), 6893–6902.
Welsh, J., McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res., 18, 7213–7218.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V. (1990). DNA polimorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res., 18, 6531–6535.
Wójcik, A.R., Laudański, Z. (1989). Planowanie i wnioskowanie statystyczne w doświadczalnictwie. PWN, Warszawa, pp. 130.
Wróblewska, K. (2013). The influence of benzyladenine and naphthalene-1-acetic on rooting and growth of Fuchsia hybrida cuttings. Acta Sci. Pol. Hortorum Cultus,
12(1), 101–113.
Zakaria, L., Kulaveraasingham, H., Guan, T.S., Abdullah, F., Wan, H.Y. (2005). Random Amplified Polymorphic DNA (RAPD) and Random Amplified Microsatellite (RAMS) of Ganoderma from Infected Oil Palm and Coconut Stumps in Malaysia. Asia Pacific J. Mol. Biol. Biotech. 13(1), 23–34.
Zaman, M., Kurepin, L., Catto, W., Pharis, P. (2015). Evaluating the use of plant hormones and biostimulators in forage pastures to enhance shoot dry biomass production by perennial ryegrass (Lolium perenne L.): biological enhancement of pasture dry biomass. J. Sci. Food Agric., 96(3), 715–726.
Zagalska-Neubauer, M., Dubiec, A. (2007). Techniques and molecular markers in the study of genetic variation birds. Not. Ornitol., 48, 193–206 (in Polish).
Anatala, T.J., Gajera, H.P., Savaliya, D.D., Domadiya, R.K., Patel, S.V., Golakiya, B.A. (2014). Molecular diversity analysis of cowpea (Vigna unguiculata L.) genotypes determined by ISSR and RAPD markers. Int. J. Agric. Environ. Biotechnol., 7, 269–276.
Bowditch, B.M., Albright, D., Williams, J., Braun, M.J. (1993). The use of randomly amplified polymorphic DNA markers in comparative genome studies. In: Methods in enzymology, vol. 224. Molecular evolution: producing the biochemical data, Zimmer, E.A., White, T.J., Cann, R.L., Wilson, A.C. (eds). Academic Press, San Diego, 294–309.
Burg, K., Helmersson, A., Bozhkov, P., von Arnold, S. (2007). Developmental and genetic variation in nuclear microsatellite stability during somatic embryogenesis
in pine. J. Exp. Bot., 58(3), 687–698.
Dobrzański, A., Anyszka, Z., Elkner, K. (2008). Reakcja marchwi na ekstrakty pochodzenia naturalnego z alg z rodzaju Sargassum – AlgaminoPlant i z leonardytu –
HumiPlant. J. Res. Appl. Agric. Engineer., 53, 53–58.
Dodgson, J.B., Cheng, H.H., Okimoto, R. (1997). DNA marker technology: a revolution in animal genetics. Poult. Sci., 76, 1108–1114.
Drahn, S.R. (2007). Auxin application via foliar sprays. Comb. Proc. Int. Plant Propag. Soc., 57, 44–48.
du Jardin, P. (2015). Plant biostimulants: definition, concept, main categories and regulation. Sci. Hortic., 196, 3–14.
El-Shiekh, A., Wildung, D.K., Luby, J.J., Sargent, K.L., Read, P.E. (1996). Long-term effects of propagation by tissue culture or softwood single-node cuttings on growth habit, yield, and berry weight of ‘Northblue’ blueberry. J. Am. Soc. Hortic. Sci., 121(2), 339–342.
Gaafar, R.M., Saker, M.M. (2006). Monitoring of cultivars identity and genetic stability in strawberry varieties grown in Egypt. World J. Agric. Sci., 2(1), 29–36.
Gajc-Wolska, J., Kowalczyk, K., Nowecka, M., Mazur, K., Metera, A. (2012). Effect of organic-mineral fertilizers on the yield and quality of endive (Cichorium endivia L.). Acta Sci. Pol. Hortorum Cultus, 11(3), 189–200.
Gajdošová, A., Ostrolucká, M.G., Libiaková, G., Ondrušková, E., Šimala, D. (2006). Microclonal propagation of Vaccinium sp. and Rubus sp. and detection of genetic
variability in culture in vitro. J. Fruit Ornam. Plant Res., 14, 103–118.
Gu, W.K., Acland, G.M., Aguirre, G.D., Ray, K. (1997). Evaluation of RAPD analysis for identification of polymorphisms in canine genomic DNA. Anim. Biotech., 8, 207–219.
Hazarika, B.N. (2003). Acclimatization of tissue-cultured plants. J. Curr. Res. Sci., 85, 1704–1711.
Jain, S.M. (2001). Tissue culture-derived variation in crop improvement. Euphytica, 118, 153–166.
Khan, W., Rayirath, U.P., Subramanian, S., Jithesh, M.N., Rayorath, P., Hodges, D.M., Critchley, A.T., Craigie, J.S., Norrie, J., Prithiviraj, B. (2009). Seaweed extracts
as biostimulants of plant growth and development. J. Plant Growth Reg., 28, 386–399.
Kroin, J. (2009). Propagation of plants from cuttings using rooting solutions by foliar methods. Comb. Proc. Int. Plant Propag. Soc., 59, 437–453.
Kroin, J. (2010). Propagation of cuttings using foliar applied IBA in aqueous solutions at or after sticking. Comb. Proc. Int. Plant Propag. Soc., 60, 369–377.
Kroin, J. (2014). Development and application of foliar applied rooting solutions. Comb. Proc. Int. Plant Propag. Soc., 64, 431–436.
Kruk, K., Zapalska, M., Węgorek, T., Kowalczyk, K. (2012). Ocena zróżnicowania genetycznego dwóch populacji sosny zwyczajnej (Pinus sylvestris L.) z okolic Puław. Annales UMCS, sec. E, Agricultura, 67(2), 1–11.
Kumar, G., Sahoo, D. (2011). Effect of seaweed liquid extract on growth an yield of Triticum aestivum var. Pusa Gold. J. Appl. Phys., 23, 251–255.
Litwińczuk, W., Szczerba, G., Wrona, D. (2005). Field performance of highbush blueberries (Vaccinium corymbosum L.) cv. ‘Herbert’ propagated by cuttings
and tissue culture. Sci. Hortic., 106, 162–169.
Matysiak, K., Kaczmarek, S., Kierzek, R., Kardasz, P. (2010). Effect of seaweeds extracts and humic and fulvic acids on the germination and early growth of winter oilseed rape (Brassica napus L.). J. Res. Appl. Agric. Engineer., 55, 28–32.
Masari, M. (2009). Mass propagation of highbush blueberry using short cut dormant shoots. Jpn. Soc. Hortic. Sci., 8(2), 161–167.
Meiners, J., Schwab, M., Szankowski, I. (2007). Efficient in vitro regeneration for Vaccinium species. Plant Cell Tiss. Organ Cult., 89, 169–176.
Mugnai, S., Azzarello, E., Pandolfi, C., Salamagne, S., Briand, X., Mancuso, S. (2008). Enchancement of ammonium and potassium root influxes by the application
of marine bioactive substances positively affects Vitis vinifera plant growth. J. Appl. Phycol., 20, 177–182.
Ostrolucká, M.G., Gajdošová, A., Libiaková, G., Hrubíková, K., Bežo, M. (2007). Protocol for micropropagation of selected Vaccinium sp. Jain, S.M. Häggman, H. (eds). Protocols for Micropropagation of Woody Trees and Fruits. Springer, Berlin–Heidelberg–New York, 445–455.
Pacholczak, A., Nowakowska, K. (2015). The ex vitro rooting of blueberry (Vaccinium corymbosum L.) mictrocuttings. Folia Hortic., 27(2), 145–150.
Pacholczak, A., Szydło, W., Petelewicz, P., Szulczyk, K. (2013). The effect of Algamino Plant on rhizogenesis in stem cuttings of Physocarpus opulifolius ‘Dart’s Gold’ and ‘Red Baron’. Acta Sci. Pol. Hortorum Cultus, 12(3), 105–116.
Przybysz, A., Wrochna, M., Słowiński, A., Gawrońska, H. (2010). Stimulatory effect of Asahi SL on selected plant species. Acta Sci. Pol. Hortorum Cultus, 9(2), 53–63.
Read, P.E., Wildung, D.K., Hartley, C.A., Sandahal, J.G. (1989). Field performance of in vitro propagated ‘Northblue’ blueberries. Acta Hortic., 241, 191–194.
Rodriguez, J.M., Berke, T., Engle, L., Nienhuis, J. (1999). Variation among and within Capsicum species revealed by RAPD markers. Theor. Appl. Genet., 99, 147–156.
Ružic, D., Vujović, T., Libiakova, G., Cerović, R., Gajdošová, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). J. Berry Res., 2, 97–103.
Snedecor, G.W., Cochran, W.G. (1967). Statistical methods. The Iowa State University Press, Ames, Iowa, 593 pp.
Spethmann, W. (1998). Factor affecting rooting of difficult to root plants. Comb. Proc. Int. Plant Propag. Soc., 48, 200–205.
Strik, B. (2006). Blueberry production and research trends in North America. Acta Hortic., 715, 173–184.
Sunnocks, P. (2000). Efficient genetic markers for population biology. Trends Ecol. Evol., 15, 199–203.
Vescan, L.A., Pamfil, D., Clapa, D., Fira, A., Sisea, C.R., Pop, I.F., Petricele, I.V., Ciuzan, O., Pop, R. (2012). Efficient micropropagation protocol for highbush blueberry
(Vaccinium corymbosum L.) cv. ‘Elliot’. Rom. Biotech. Lett., 17(1), 6893–6902.
Welsh, J., McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res., 18, 7213–7218.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V. (1990). DNA polimorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res., 18, 6531–6535.
Wójcik, A.R., Laudański, Z. (1989). Planowanie i wnioskowanie statystyczne w doświadczalnictwie. PWN, Warszawa, pp. 130.
Wróblewska, K. (2013). The influence of benzyladenine and naphthalene-1-acetic on rooting and growth of Fuchsia hybrida cuttings. Acta Sci. Pol. Hortorum Cultus,
12(1), 101–113.
Zakaria, L., Kulaveraasingham, H., Guan, T.S., Abdullah, F., Wan, H.Y. (2005). Random Amplified Polymorphic DNA (RAPD) and Random Amplified Microsatellite (RAMS) of Ganoderma from Infected Oil Palm and Coconut Stumps in Malaysia. Asia Pacific J. Mol. Biol. Biotech. 13(1), 23–34.
Zaman, M., Kurepin, L., Catto, W., Pharis, P. (2015). Evaluating the use of plant hormones and biostimulators in forage pastures to enhance shoot dry biomass production by perennial ryegrass (Lolium perenne L.): biological enhancement of pasture dry biomass. J. Sci. Food Agric., 96(3), 715–726.
Zagalska-Neubauer, M., Dubiec, A. (2007). Techniques and molecular markers in the study of genetic variation birds. Not. Ornitol., 48, 193–206 (in Polish).
Karolina Nowakowska
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, Poland
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, Poland
Andrzej Pacholczak
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, Poland
Department of Ornamental Plants, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), 166 Nowoursynowska str., 02-787 Warszawa, Poland
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