Abstract
The objective of this study was to test yield and fruit quality of the red raspberry ‘Meeker’ and the blackberry ‘Čačanska Bestrna’ propagated using the standard method (ST) and in vitro, by tissue culture (TC). The monitored parameters included the total number of canes, cane number per one metre of the planted row, yield per cane (raspberry) or per bush (blackberry) and total yield. Monitoring fruit quality parameters included the weight and dimensions of fruit, the number, weight and dimensions of individual drupelets and weight of drupelet seeds. No significant differences were determined either for the total number of canes and/or the cane number per row metre in plants originating from both types of planting material in both genotypes. Significant differences were observed to the advantage of the raspberry ST plants, in the total yield, as well as the fruit weight in the blackberry ST plants. A significantly higher weight of drupelet seeds was observed in TC plants of both genotypes. Concerning the organoleptic assessment of fruits, no significant differences were recorded between fruits coming from ST and TC plants.
References
Bite, A., Petrevica, L. (2002). The influence of in vitro propagation on the field behavior of red raspberry variety ‘Norna’. Acta Hortic., 585, 615–619.
Cerović, R., Ružić, Đ. (1989). Micropropagation of strawberry cvs Čačanska rana and Senga Sengana. Pomological-biochemical characteristics of micropropagated plants. Acta Hortic., 265, 353–358.
Cerović, R., Leposavić, A. (2011). Current state and perspectives of small fruit production in the Republic of Serbia. J. Mt. Agric. Balk., 14, 1156–1170.
EPPO (2009). Certification scheme for Rubus. PM 4/10(2) EPPO Bulletin, 39, 271–277. doi: 10.1111/j.1365-2338.2009.02308.
Gantait, S., Mandal, N., Prakash, K.D. (2010). Field performance and molecular evaluation of micropropagated strawberry. Recent Res. Sci. Technol., 2(5), 12–16.
Georgieva, M., Kondakova, V., Dragoyski, K., Georgiev, D., Naydenova, G. (2009). Comparative study of raspberry cv. Balgarski Rubin propagated by classical and in vitro methods. J. Pomology, 43, 81–86.
Isac, V., Popescu, A. (2009). Protocol for in vitro micropropagation of raspberry and plant regeneration by organogenesis. In: A guide to some in vitro techniques – Small fruits, Mezzetti, B., Ružić, Đ., Gajdosova, A. (eds.), COST Action 863, Brussels (Belgium), 14–23.
Leposavić, A., Blagojević, M., Rakićević, M. (2003). Propagation of blackberry cv Čačanska Bestrna by softwood cuttings. Cont. Agri., 52, 85–87.
Leposavić, A., Đurović, D., Keserović, Z., Popović, B., Mitrović, O., Miletić, N., Magazin, N. (2013). Evaluation of raspberry cultivars grown in the western Serbia region. Hortic. Sci. (Prague), 40, 1–7 .
Leposavić, A., Đurović, D., Keserović, Z., Jevremović, D. (2015). Vegetative and yield potential of cultivars and selection of raspberry cultivated in conditions of Western Serbia. Bulg. J. Agric. Sci., 21, 153‒159.
Lewandowski, M., Żurawicz, E., Pruski, K. (2015). The effects of the growing season extension on Polish primocane-fruiting raspberry cultivars. Hortic. Sci. (Prague), 42, 203–208.
Official Gazette RS No. 54/93, 35/94, 43/94; Official Gazette RS No. 135/04; 18/05 and 101/2005: Law on seed and planting material.
Popov, S. (1993). Behaviour of the micropropagated Stanley plum cultivar in the plantation. Biotechnol. Biotec. Eq., 7, 21–23.
Ružić, Đ., Cerović, R., Nikolić, M. (1991). Field performance of micropropagated sour cherry cv. Šumadinka. J. Pomology, 25, 19–26. (In Serbian).
Ružić, Đ., Cerović, R. (1998). A rapid method of blackberry propagation. Acta Agric. Serb., 6, 55–61.
Ružić, Đ., Cerović, R. (2001). Field performance of micropropagated plum cv. Požegača. Acta Agric. Serb., 11, 3–9.
Ružić, Đ., Lazić, T. (2006). Micropropagation as means of rapid multiplication of newly developed blackberry and black currant cultivars. Agric. Conspec. Sci., 71(4), 149–153.
Ružić, Đ., Vujović, T., Cerović, R. (2009). Protocol for in vitro micropropagation of blackberry, and plant regeneration by organogenesis. In: A guide to some in vitro techniques – Small fruits, Mezzetti, B., Ružić, Đ., Gajdosova, A. (eds.). COST Action 863, Brussels (Belgium), 24–29.
Ružić, Đ., Leposavić, A., Cerović, R., Karaklajić-Stajić, Ž., Vujović, T., Miletić, N., Žurawicz, E. (2013). Physiological properties of raspberry and blackberry propagated by in vitro micropropagation and standard techniques. J. Pomology, 47, 55–61. (In Serbian). Strik, B.C., Clark, J.R., Finn, C.E., Bañados, P. (2007). Worldwide production of blackberries, 1995 to 2005 and predictions for growth. HortTechnol., 17, 205–213.
Tsao, W.V., Reed, B.M. (2002). Gelling agents, silver nitrate, and sequestrene iron influence adventitious shoot and callus formation from Rubus leaves. In Vitro Cell. Dev. Biol. Pl., 38, 29–32.
Velchev, V., Toshkov, A. (2005). Reproductive performances of micropropagated raspberry plants. J. Pomology, 39, 25–32.
Zawadzka, M., Orlikowska, T. (2006). The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants. Plant Cell Tiss. Org. Cult., 85, 145–149.
Zawadzka, M., Orlikowska, T. (2009). Influence of FeEDDHA on in vitro rooting and acclimatisation of red raspberry (Rubus ideaus L.) in peat and vermiculite. J. Hort. Sci. Biotechnol., 84, 599–603.
Żebrowska, J., Kaczmarska, E., Gawroński, J. (2015). Comparative studies on the agronomic value of in vitro and conventionally propagated strawberry (Fragaria × ananassa Duch.) plants. Acta Sci. Pol. Hortorum Cultus, 14(3), 25–35.
Downloads
Download data is not yet available.
