ANTIFUNGAL ACTIVITY OF SOME PLANT EXTRACTS AGAINST Alternaria alternata (Fr.) Keissl. IN THE BLACK CURRANT CROP (Ribes nigrum L.)
Tatiana Eugenia Sesan
University of Bucharest, Bucharest, Romania, National Research-Development Institute for Chemistry and Petrochemistry Bucharest, RomaniaElena Enache
University of Bucharest, Bucharest, RomaniaBeatrice Michaela Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, RomaniaMaria Oprea
Research-Development Institute of Plant Protection Bucharest, RomaniaFlorin Oancea
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, RomaniaCristian Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, RomaniaAbstract
There were tested and screened, in vitro and in vivo, for the first time in Romania, nine respectively six plant extracts manufactured by Hofigal S.A. against Alternaria alternata (strain Aa 20) isolated from blackcurrant (Ribes nigrum L.). The highest in vitro activity (efficacy between 70% and 100%) was recorded for Satureja hortensis and Valeriana officinalis extracts at 20 and 10% concentrations. A good inhibitory activity on mycelial growth (efficacy between 54.3 and 88.6%) has been noticed for Allium sativum, Mentha sp. Rosmarinus officinalis, Tagetes patula extracts (at 10 and 20%). No efficacy was noticed for Artemisia dracunculus ‘sativa’. The extract obtained from Hyssopus officinalis inhibited the mycelial growth of A. alternata only at 20%, with 57.1% efficacy. In field trials, Valeriana officinalis and Satureja hortensis extracts have been very effective in limiting Alternaria disease severity in blackcurrant applied at 10%, compared to untreated control. This data confirmed their strong antifungal potential, both in vitro and in vivo. Based on our results, plant extracts with highly efficacy could be an alternative in the protection of blackcurrant as medicinal crop against Alternaria leaf spot and fruit rot especially in organic horticulture system.
Keywords:
Black currant, Alternaria alternata, plant extracts, organic horticulture, RomaniaReferences
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Bhosale, S.B., Jadhav, D.S., Patil, B.Y., Chavan, A.M. (2014). Bioefficacy of plant extract on Alternaria leaf spot of soybean (Glycine max (L.) Merr. Indian J. Appl. Res., 4(11), 79–81.
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Chaudhary, R.F., Patel, R.L., Chaudhari, S.M. 2003. In vitro evaluation of different plant extracts against Alternaria alternata causing early blight of potato. Potato J., 30(5), 141–142.
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Neeraj, B., Verma, S. (2010). Alternaria diseases of vegetables and new approaches for its control. Asian J. Exp. Biol. Sci., 1(3), 681–692.
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Ramjegathesh, R., Ebenezar, E.G., Muthusami, M. (2011). Management of onion leaf blight by Alternaria alternata by botanicals and biocontrol agents. Plant Pathol. J., 10(4), 192–196.
Ranaware, A., Singh, V., Nimbkar, N. (2010). In vitro antifungal studies on the efficacy of some plant extracts for inhibition of Alternaria carthami fungus. Indian J. Nat. Prod. Res., 1(3), 384–386.
Rizatti, M.A., Schwan-Estrada, K.R.F., Cruz, M.E.S., Stangarlin, J.R. (2000). Effect of crude extract of Achillea millefolium and Artemisia absinthium on Alternaria spp. Anuário CCA/UEM.
Rodino, S., Butu, M., Petrache, P., Butu, A., Cornea, C.P. (2014). Antifungal activity of four plants against Alternaria alternata. Sci. Bull. Ser. F., Biotechnol., 18, 60–64
Saha, S., Walia, S., Kundu, A., Kumar, B., Joshi, D. (2012). Antifungal acetylinic thiophenes from Tagetes minuta: Potential. Biopest. J. Appl. Bot. Food Qual., 85, 207 – 211.
Sadeghi-Nejad, K., Shiravi, F., Ghanbari, S., Alinejadi, M., Zarrin, M. (2010). Antifungal activity of Satureja khuzestanica (jamzad) leaves extracts. Jundishapur J. Microbiol., 3(1), 36–40.
Singh, S., Srivastava, S., Mishra, J., Raaj, R., Sinha, A. (2014). Evaluation of some plant extracts against predominant seed mycoflora of mungbean Vigna radiata (L.) Wilczek seed. Life Sci. Leaflets, 51, 83–89.
Sobhy II, A.H., Abo-Elyousr, K.A.M., Abdel-Rahim, I.R. (2013). Effect of certain plant extracts to control purple blotch disease of onuion plants (Allium cepa L.). J. Plant Physiol. Path., 1(4), 1–4.
Stangarlin, J.R., Kuhn, O.J., Assi, L., Schwan-Estrada, K.R.F. (2011). Control of plant diseases using extracts from medicinal plants and fungi. In: Science against microbial pathogens communicating current research and technological advances, Mendez-Vilas, A. (ed.). Formatex, 1033–1042.
Swami, C.S., Alane, S.K. (2013). Efficacy of some botanicals against seed-borne fungi of green gram (Phaseolus aureus Roxb). Biosci. Discov., 4(1), 107–110.
Sesan, T.E., Enache, E., Iacomi, B.M., Oprea, M., Oancea, F., Iacomi, C. (2015). Antifungal activity of some plant extracts against Botrytis cinerea Pers. in the blackcurrant crop (Ribes nigrum L.). Acta Sci. Pol. Hortorum Cultus, 14(1), 29–43.
Taskeen-Un-Nisa, Wani, H.A., Mir, R.A. (2010). Antimycotic activity of plant extracts on the spore germination of some pathogenic fungi. Mycopath, 8(2), 65–69.
Vagiri, M. (2012). Black currant (Ribes nigrum) – an insight into the crop. A synopsis of a PhD study. Swedish Univ. of Agricultural Sciences, 58 p. ISSN 1654-3580.
Waghmare, M.B. (2014). Ecofriendly approaches for the management of Alternaria zinniae Pape. causing flower blight of Tagetes erecta L. Curr. Biot., 8(3), 317–321.
Yazgi, M., Awad, D., Jreikous, B. (2015). Screening of the antifungal activity of plant Mentha longifolia crude extracts against two fungi Alternaria citri and Fusarium moniliforme. J. Entomol. Zool. Stud., 3(2), 359–364.
Begum, F., Mahal F., Kamal, S. (2010). Inhibition of spore germination and mycelial growth of three fruit pathogens using some chemical fungicides and botanical extracts. J. Life Sci., 5, 23–27.
Bhosale, S.B., Jadhav, D.S., Patil, B.Y., Chavan, A.M. (2014). Bioefficacy of plant extract on Alternaria leaf spot of soybean (Glycine max (L.) Merr. Indian J. Appl. Res., 4(11), 79–81.
Carvalho, D.D.C., Alves, E., Camargos, R.B., Oliveira, D.F., Scolforo, J.R.S., Carvalho, D.A., Batista, T.R.C. (2011). Plant extracts to control Alternaria alternata in Murcott tanger fruits. Rev. Iberoam. Micol., 28(4), 173–178.
Chaudhary, R.F., Patel, R.L., Chaudhari, S.M. 2003. In vitro evaluation of different plant extracts against Alternaria alternata causing early blight of potato. Potato J., 30(5), 141–142.
Chetana, B.S., Ganeshan, G., Rao, A.S., Bellishree, K. (2012). In vitro evaluation of plant extracts, bioagents and fungicides against Alternaria porri (Ellis) Cif. causing purple blotch disease of onion. Pest Manag. Hortic. Ecosyst., 18(2), 194–198.
Dellavalle, P.D, Cabrera, A., Alem, D., Larranga, P., Ferreira, F., Rizza, M.D. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chil. J. Agricult. Res., 71(12), 231–239.
Ganie, S.A., Ghani, M.Y., Nissar, Q., Shabir-u-Rehman. (2013). Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. African J. Microbiol. Res., 7(34), 4397–4402.
Haggag, W.M., El Khair, H.A.I. (2007). Application of some natural compounds for management of potato late and early blights. J. Food Agric. Environ., 5(2), 157–163.
Hedley, P.E., Russell, J.R., Jorgensen, L., Gordon, S., Morris, J. A., Hackett, C.A., Cardle, L., Brennan, R. (2010). Candidate genes associated with bud dormancy release in blackcurrant (Ribes nigrum L.). BMC Plant Biol., 10 (202), 1–13.
Itako, A.T., Schwan-Estrada, K.R.F., Tolentino Junior, J.B., Stangarlin, J.R., Cruz, M.E.S. (2008). Antifungal activity and protection of tomato plants by extracts of medicinal plants. Trop. Plant Pathol., 33, 241–244.
Ivănescu, B. (2010). Studiul fitochimic al unor compusi din speciile Artemisia absinthium, A. vulgaris si A. annua recoltate din flora spontană. Rezumat teză de doctorat. Univ. de Medicină si Farmacie “Gr. T. Popa”, Facultatea de Farmacie [in Romanian].
Kantwa, S.L., Tetarwal, J.P., Shekhawat, K.S. (2014). In vitro effect of fungicides and plant extracts against Alternaria alternata causing leaf blight of groundnut. IOSR J. Agricult. Vet. Sci., 7(6), 28–31.
Khurana, A.K., Metha, N., Sangwan, M.S. (2005). Variability in sensitivity of Alternaria brassicae isolates to plant extracts. J. Mycol. Plant. Pathol., 35, 76–77.
Mesta, R.K., Benagi, V.I., Kulkarni, S., Shankergoud, I. (2009). In vitro evaluation of fungicides and plant extracts against Alternaria helianthi causing leaf blight of sunflower. Karnataka J. Agric. Sci., 22(1), 111–114.
Nashwa, M.A.S., Abo-Elyousr, A.M.K. (2012). Evaluation of various plant extracts against the early blight disease of tomato under greenhouse and field conditions. Plant Prot. Sci., 48(2), 74–79.
Neeraj, B., Verma, S. (2010). Alternaria diseases of vegetables and new approaches for its control. Asian J. Exp. Biol. Sci., 1(3), 681–692.
Pattnaik, M.M., Kar, M., Sahu, R.K. (2012). Bioefficacy of some plant extracts on growth parameters and control of diseases in Lycopersicon esculentum. Asian J. Plant Sci. Res., 2(2), 129–142.
Raja, P. (2010). Fungitoxic properties of plant extracts against Alternaria tenuissima: new sp. infecting eggplant in India. Indian Phytopath., 63(1), 45–46.
Rama Devi, P., Tanuja Pryia, B., Sunitha, P., Rajasekhar, M. (2015). Bioefficacy of plant extracts against Alternaria alternata, leaf blight pathogen of Senna (Cassia angustifolia). Internat. J. Multidisc. Adv. Res. Tr., 2(1), 7–11.
Ramjegathesh, R., Ebenezar, E.G., Muthusami, M. (2011). Management of onion leaf blight by Alternaria alternata by botanicals and biocontrol agents. Plant Pathol. J., 10(4), 192–196.
Ranaware, A., Singh, V., Nimbkar, N. (2010). In vitro antifungal studies on the efficacy of some plant extracts for inhibition of Alternaria carthami fungus. Indian J. Nat. Prod. Res., 1(3), 384–386.
Rizatti, M.A., Schwan-Estrada, K.R.F., Cruz, M.E.S., Stangarlin, J.R. (2000). Effect of crude extract of Achillea millefolium and Artemisia absinthium on Alternaria spp. Anuário CCA/UEM.
Rodino, S., Butu, M., Petrache, P., Butu, A., Cornea, C.P. (2014). Antifungal activity of four plants against Alternaria alternata. Sci. Bull. Ser. F., Biotechnol., 18, 60–64
Saha, S., Walia, S., Kundu, A., Kumar, B., Joshi, D. (2012). Antifungal acetylinic thiophenes from Tagetes minuta: Potential. Biopest. J. Appl. Bot. Food Qual., 85, 207 – 211.
Sadeghi-Nejad, K., Shiravi, F., Ghanbari, S., Alinejadi, M., Zarrin, M. (2010). Antifungal activity of Satureja khuzestanica (jamzad) leaves extracts. Jundishapur J. Microbiol., 3(1), 36–40.
Singh, S., Srivastava, S., Mishra, J., Raaj, R., Sinha, A. (2014). Evaluation of some plant extracts against predominant seed mycoflora of mungbean Vigna radiata (L.) Wilczek seed. Life Sci. Leaflets, 51, 83–89.
Sobhy II, A.H., Abo-Elyousr, K.A.M., Abdel-Rahim, I.R. (2013). Effect of certain plant extracts to control purple blotch disease of onuion plants (Allium cepa L.). J. Plant Physiol. Path., 1(4), 1–4.
Stangarlin, J.R., Kuhn, O.J., Assi, L., Schwan-Estrada, K.R.F. (2011). Control of plant diseases using extracts from medicinal plants and fungi. In: Science against microbial pathogens communicating current research and technological advances, Mendez-Vilas, A. (ed.). Formatex, 1033–1042.
Swami, C.S., Alane, S.K. (2013). Efficacy of some botanicals against seed-borne fungi of green gram (Phaseolus aureus Roxb). Biosci. Discov., 4(1), 107–110.
Sesan, T.E., Enache, E., Iacomi, B.M., Oprea, M., Oancea, F., Iacomi, C. (2015). Antifungal activity of some plant extracts against Botrytis cinerea Pers. in the blackcurrant crop (Ribes nigrum L.). Acta Sci. Pol. Hortorum Cultus, 14(1), 29–43.
Taskeen-Un-Nisa, Wani, H.A., Mir, R.A. (2010). Antimycotic activity of plant extracts on the spore germination of some pathogenic fungi. Mycopath, 8(2), 65–69.
Vagiri, M. (2012). Black currant (Ribes nigrum) – an insight into the crop. A synopsis of a PhD study. Swedish Univ. of Agricultural Sciences, 58 p. ISSN 1654-3580.
Waghmare, M.B. (2014). Ecofriendly approaches for the management of Alternaria zinniae Pape. causing flower blight of Tagetes erecta L. Curr. Biot., 8(3), 317–321.
Yazgi, M., Awad, D., Jreikous, B. (2015). Screening of the antifungal activity of plant Mentha longifolia crude extracts against two fungi Alternaria citri and Fusarium moniliforme. J. Entomol. Zool. Stud., 3(2), 359–364.
Tatiana Eugenia Sesan
University of Bucharest, Bucharest, Romania, National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
University of Bucharest, Bucharest, Romania, National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
Elena Enache
University of Bucharest, Bucharest, Romania
University of Bucharest, Bucharest, Romania
Beatrice Michaela Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
Maria Oprea
Research-Development Institute of Plant Protection Bucharest, Romania
Research-Development Institute of Plant Protection Bucharest, Romania
Florin Oancea
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
Cristian Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
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