USE OF RAPD-PCR AND ITS MARKERS FOR IDENTIFICATION OF Diaporthe/Phomopsis FROM FRUIT TREES IN SOUTH-EASTERN POLAND

Barbara Abramczyk

Institute of Soil Science and Plant Cultivation – State Research Institute, Puławy

Ewa Król

University of Life Sciences in Lublin



Abstract

In recent years, it has been reported the increasing harmfulness of Diaporthe/Phomopsis spp. in the orchards and nurseries worldwide. They cause nonspecific
symptoms which in combination with the morphological similarity of the cultures and difficult sporulation creates problems with their correct identification using classical methods. This implies the need to integrate advanced molecular techniques to identify the strain on the species level. The aim of this research was to determine the identity and genetically characterize forty isolates of Diaporthe/Phomopsis obtained in 2010–2012 from the shoots of selected fruit trees grown in the south-eastern Poland. Using the RAPD-PCR technique the genetic similarity within studied population has been demonstrated irrespectively of the origin and type of the host plant from which the isolates derived. Based on the comparative analysis of the non-coding sequence of the ITS regions (ITS1, 5.8S rDNA, ITS2) with the sequences available in NCBI database the studied Diaporthe isolates were identified as Diaporthe eres species complex not described previously on fruit trees under Polish conditions.

Keywords:

Diaporthe eres species complex, molecular identification, orchard plants, fungi

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Published
2016-12-31



Barbara Abramczyk 
Institute of Soil Science and Plant Cultivation – State Research Institute, Puławy
Ewa Król 
University of Life Sciences in Lublin



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