IMPROVEMENT OF Chrysanthemum × grandiflorum (Ramat.) Kitam. ENCAPSULATION-DEHYDRATION CRYOPRESERVATION PROTOCOL
Małgorzata Zalewska
University of Technology and Life Sciences in BydgoszczDariusz Kulus
University of Technology and Life Sciences in BydgoszczAbstract
To optimize the protocol, the shoot tips of Chrysanthemum × grandiflorum (Ramat.) Kitam. radiomutants have been cryopreserved using the encapsulationdehydration technique, following different approaches. In the experiment the influence of abscisic acid – ABA (0; 10; 20 and 30 μM), sucrose concentration (0.09; 0.25 and 0.5 M) and temperature (5ºC or 22ºC) during preculture were tested. Furthermore two dehydration methods, gradual and one-step, were considered. Since the best results were reported with 10 μM ABA + 0.09 M sucrose at room temperature and gradual dehydration, only this combination was selected for the next desiccation-adjustment step. From the time of air desiccation under laminar air-flow applied (0; 1; 2; 3; 4 and 5 hours), 3 hours provided
the best results. Due to the growth inhibition on the hormone-free medium, the addition of plant growth regulators during that phase was also tested, confirming the need to apply cytokinins into the medium.
Keywords:
mutant, ABA, sucrose, preculture, desiccation, regrowthReferences
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University of Technology and Life Sciences in Bydgoszcz
University of Technology and Life Sciences in Bydgoszcz
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