Elżbieta Paduch-Cichal

Warsaw University of Life Sciences

Maria Chorodorska

Warsaw University of Life Sciences

Elżbieta Kalinowska

Warsaw University of Life Sciences

Beata Komorowska

Research Institute of Horticulture, Skierniewice


Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in
various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].


blueberry cultivars, BlScV, DAS-ELISA, RT-PCR

Boom R., Sol C.J.A., Salimans M.M.M., Jansen C.L., Wertheim-Van Dillen P.M.E., Van Der Nordaa J., 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495–503.
Bristow P.R., Martin R.R., 1987. A virus associated with a new blight disease of highbush blueberry. Phytopathology 77, 1721–1722.
Bristow P.R., Martin R.R., Windom G.E., 2000. Transmission, field spread, cultivar response, and impact on yield in highbush blueberry infected with Blueberry scorch virus. Phytopathology 90, 474–479.
Caruso F.L., Ramsdell D.C., 1995. Compendium of blueberry and cranberry diseases. APS Press, St Paul, MN.
Cavileer T.D., Halpern B.T., Lawrence D.M., Podleckis E.V., Martin R.R., Hillman B.I., 1994.
Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. J. General Virol. 75, 711–720.
Ciuffo M., Pettiti D., Gallo S., Masegna V., Turina M., 2005. First report of Blueberry scorch virus in Europe. Plant Pathol. 54, 565.
Converse R.H., Ramsdell D.C., 1982. Occurrence of tomato ringspot viruses and dagger and other nematodes associated with cultivated highbush blueberries in Oregon. Plant Disease 66, 710–712.
Garcia-Salazar C., 2002. Crop timeline for blueberries in Michigan and Indiana. Prepared for the U.S. Environmental Protection Agency.
Halpern B.T., Hillman B.I., 1996. Detection of Blueberry scorch virus strain NJ2 by reverse transcriptase-polymerase chain reaction amplification. Plant Disease 80, 219–222.
Jaswal A.S., 1990. Occurrence of blueberry leaf mottle, blueberry shoestring, tomato ringspot and tobacco ringspot virus in eleven halfhigh blueberry clones grown in New Brunswick, Canada. Canadian Plant Disease Survey 70, 113–117.
Loomis M.D., 1974. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 31, 528–544.
Łabanowska B., 2010. Blueberry pests and their control possibilities. National Conference on Science Practice: ‘Intensification of berry bushes planted by the implementation of the latest research results’. Growing blueberries, Skierniewice, 59–63.
MacDonald S.G., Martin R.R., Bristow P.R., 1991. Characterization of an ilarvirus associated with a necrotic shock reaction in blueberry. Phytopathology 81, 210–214.
Malinowski, T., 1997. Silicacapture-reverse transcription-polymerase chain reaction (SC-RT-PCR): application for the detection of several plant viruses. Diagn. Identificat. Plant Path. Develop. Plant Pathol. 11, 445–448.
Martin R.R., 2001. Appendix I. Recommended procedures for detection of viruses of small fruit crops. Acta Horticult. 551, 113–123.
Martin R.R., Bristow P.R., 1988. A carlavirus associated with Blueberry scorch disease. Phytopathology 78, 1636–1640.
Medina C., Matus J.T., Zuniga M., San-Martin C., Arce-Johnson P., 2006. Occurrence and distribution of viruses in commercial plantings of Rubus, Ribes and Vaccinium species in Chile. Ciencia e Investigación Agraria 33, 23–28.
Moretti M., Ciuffo M., Gotta P., Prodorutti D., Bragagna P., Turina M., 2011. Molecular characterization of two distinct strains of blueberry scorch virus (BlScV) in northern Italy. Arch. Virol. 156, 1295–1297.
Paduch-Cichal E., Kalinowska E., Chodorska M., Sala-Rejczak K., Nowak B., 2011. Detection and identification of viruses of highbush blueberry and cranberry using serological ELISA test and PCR technique. Acta Sci. Pol., Hortorum Cultus 10, 201–215.
Pansa M.G., Tavella L., 2008. Aphid population dynamics on highbush blueberry in relation to the spread of Blueberry scorch virus in Piedmont (NW Italy). Bull Insect 61, 205–206.
Prior R.L., Cao G., Martin A., Sofic E., McEwan J., O’Brien C., Lischner N., Ehlenfeldt M., Kalt W., Krewer G., Mainland C.M., 1998. Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity and variety Vaccinium species. J. Agricult. Food Chem. 46, 2686– 2693.
Prodorutti D., Pertot I., Giongo L., Gessler C., 2007. Highbush Blueberry: cultivation, protection, breeding and biotechnology. European J. Plant Sci. Biotech. 1, 44–56.
Sanchez-Navarro J.A., Aparicio F., Herranz M.C., Minafra A., Myrta A., Pallas V., 2005. Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. European J. Plant Pathol. 111, 77–84.
Wegener L.A., Martin R.R., Bernardy M.G., MacDonald. L., Punja Z.K., 2006. Epidemiology and strain identification of Blueberry scorch virus on highbush blueberry in British Columbia. Canadian J. Plant Pathol. 28, 250–262.
Wu X., Beecher G.R., Holden J.M., Haytowitz D.B., Gebhardt S.E., Prior R.L., 2004. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J. Agricult. Food Chem. 52, 4026–4037.


Elżbieta Paduch-Cichal 
Warsaw University of Life Sciences
Maria Chorodorska 
Warsaw University of Life Sciences
Elżbieta Kalinowska 
Warsaw University of Life Sciences
Beata Komorowska 
Research Institute of Horticulture, Skierniewice



Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.


Most read articles by the same author(s)