Abstract
Garlic (Allium sativum L.) plants may be infected in the field by viruses of the genera Potyvirus, Carlavirus and Allexivirus. These viruses are transmitted by vegetative
propagation and by vectors. Detection and identification of allexiviruses was carried out in 2011–2012. It was based on ELISA and RT-PCR assays. Samples of plant material were collected from 26 garlic production fields located in different regions of Poland. Garlic virus D, Garlic virus B and Garlic virus X were the most abundant viruses in all examined regions and were identified in 79%, 64% and 59% of all garlic samples, respectively. Garlic virus A and Garlic virus C were identified in all studied regions with low frequency. Garlic virus E was detected with 100% frequency in east-central Poland. None of the tested garlic samples were infected with Shallot virus X. Allexiviruses were always present in garlic plants in mixed infections.
References
Cafrune E.E., Balzarini M., Conci V.C., 2006. Changes in the concentration of an Allexivirus during the crop cycle of two garlic cultivars. Plant Dis. 90(10), 1293–1296.
Chen J., Zheng H.Y., Antoniw J.F., Adams M.J., Chen J.P., Lin L., 2004. Detection and classification of allexiviruses from garlic in China. Arch. Virol. 149, 435–445.
Chodorska M., Nowak P., Szyndel M.S., Paduch-Cichal E., Sala-Rejczak K., 2012. First report of Garlic virus A, Garlic virus B and Garlic virus C in garlic in Poland. J. Plant Pathol. 94, S4.100.
Dijk van P., Verbeek M., Bos L., 1991. Mite-borne virus isolates from cultivated Allium species, and their classification into two new rymoviruses in the family Potyviridae. Neth. J. Plant Pathol. 97, 381–399.
Dovas C.I., Hatziloukas E., Salomon R., Barg E., Shiboleth Y., Katis N.I., 2001. Incidence of viruses infecting Allium spp. in Greece. Phytopathology 149, 1–7.
Dovas C.I., Vovlas C., 2003. Viruses infecting Allium spp. in southern Italy. J. Plant Pathol. 85, 135.
Kalinowska E., Chodorska M., Paduch-Cichal E., Mroczkowska K., 2012. An improved method for RNA isolation using extraction kits. Acta Biochim. Pol. 59(3), 391–393.
King A.M.Q., Adams M.J., Carstens E.B., Lefkowitz E.J., 2012. Virus taxonomy: classification and nomenclature of viruses. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press: Amsterdam.
Klukáčková J., Navrátil N., Veselá M., Havránek P., Šafářová D., 2004. Occurrence of garlic viruses in the Czech Republic. Acta Fytotech. Zootech. 7, 126–128.
Koo B.J., Kang S.C., Chang M.U., 2002. Survey of garlic virus disease and phylogenetic characterization of garlic viruses of genus Allexivirus isolated in Korea. J. Plant Pathol. 18, 237–243.
Lanzoni C., Ratti C., Turina M., Pisi A., Tedeschi P., Autonell C.R., 2006. Molecular characterization of Allexiviruses from garlic in Italy. J. Plant Pathol. 88, 47.
Lot H., Chovelon V., Souche S., Delecolle B., 1998. Effects of Onion yellow dwarf and Leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Dis. 82, 1381–1385.
Mavric I., Ravnikar M., 2005. A carlavirus serologically closely related to Carnation latent virus in Slovenian garlic. Acta Agric. Slov. 85, 343–349.
Perotto M.C., Cafrune E.E., Conci V.C., 2010. The effect of additional viral infections on garlic plants initially infected with Allexiviruses. Eur. J. Plant Pathol. 126(4), 489–495.
Sanchez-Navarro J.A., Aparicio F., Herranz M.C., Minafra A., Myrta A., Pallas V., 2005. Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur. J. Plant Pathol. 111, 77–84.
Shahraeen N., Lesemann D.E., Ghtbi T., 2008. Survey for viruses infecting onion, garlic and leek crops in Iran. OEPP/EPPO Bull. 38, 131–135.
Smékalová K., Stavlíková H., Dusek K., 2010. Distribution of viruses in the garlic germplasm collection of the czech republic. J. Plant Pathol. 92(1), 273–274.
Sumi S., Tsuneyoshi T., Fututani H., 1993. Novel rod-shaped viruses isolated from garlic, Allium sativum, possessing a unique genome organization. J. Gen. Virol. 74, 1879–1885.
Tabanelli D., Bertaccini A., Bellardi M.G., 2004. Molecular detection of filamentous viruses infecting garlic from different geographic origins. J. Plant Pathol. 86(4), 335–335.
Van Dijk P., 1993. Survey and characterization of potyviruses and their astrains of Allium species. Neth. J. Plant Pathol. 99, 1–48.
Yamashita K., Sakai J., Hanada K., 1996. Characterization of a new virus from garlic (Allium sativum L.), garlic mite borne mosaic virus. Ann. Phytopathol. Soc. Japan 62, 483–489.
Downloads
Download data is not yet available.