Duygu Ayvaz Sönmez

Yaltır A.Ş., Sarıhuğlar mah., Adana, Turkey

Ebru Kafkas

University of Çukurova, Balcalı, Adana, Turkey


The cultivated strawberry (Fragaria × ananassa Duch.), a member of the Rosaceae, is the most important soft fruit in the world. In vitro micropropagation is an
important tool for clonal multiplication and is commonly applied for induction of somaclonal variation to create genetic variability. Optimized regeneration protocols and high efficiency are also important for gene transformation for improving new cultivars. For this purpose, in this study it was aimed to optimize a regeneration protocol for ‘Festival’ and ‘Rubygem’ strawberry varieties. Leaf disks and stipules from mature in vitro grown strawberry plants of ‘Festival’ and ‘Rubygem’ varieties (Fragaria × annanasa Duch.) were used as explant sources. Mc Cowns Woody plants including vitamins were used as a basal medium. Different concentrations of TDZ (Thidiazuron) (0, 1, 2, 3 and 4 mg l-1) and IBA (Indole-3-butyric acid) (0, 0.2 and 0.4 mg l-1) and the efficiency of 15 days dark treatment on the regeneration from leaf disks and stipules were also tested. Our results suggested that leaf stipules, a high concentration of TDZ, AgNO3 and a dark treatment in culture medium successfully resulted in the induction of regeneration.


Strawberry, in vitro, organogenesis, regeneration, AgNO3

Barcelo M., El-Mansouri I., Mercado J.A., Quesada M.A., Alfaro F.P., 1998. Regeneration and transformation via Agrobacterium tumefaciens of the strawberry cultivar Chandler. Plant Cell Tiss. Organ Cult. 54, 29–36.
Beyer E.M.,1976. A potent inhibitor of ethylene action in plants. Plant Physiol., 58, 268–271.
Boxus P., Damiano C., Brasseur E., 1989. Strawberry. In: Handbook of Plant Cell Culture, (Eds. P. Ammirato, D. Evans, W. Sharp, Y. Yamada), New York, Mecnillan, 453–486.
Cao X., Hammerschlag F.A., 2000. Improved shoot organogenesis from leaf explants of highbush blueberry. Hort Sci., 35, 945–947.
Capelle S.C., Mok D.W.S., Kirchner S.C., Mok M.C., 1983. Effects of thiadiazron on cytokinin autonomy and metabolism of N6- (2-isopentyl) (8-14C) adenosine in callus tissue of Phaseolus lunatus L. Plant Physiol., 73, 796–802.
Chien-Ying K., Al-Abdulkarim A.M., Al-Jowid S.M., Al-Baiz A., 2009. An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. African J. Biotech., 8, 2611–2615.
Debnath S.C., 2005. A two-step procedure for adventitious shoot regeneration from in-vitroderived lingonberry leaves: Shoot induction with TDZ and shoot elongation using zeatin. Hort. Sci., 40, 189–192.
Debnath S.C., 2006. Zeatin overcomes thidiazuron-induced inhibition of shoot elongation and promotes rooting in strawberry culture in vitro. J. Hortic. Sci. Biotech., 81, 349–354.
Eapen S., George L., 1997. Plant regeneration from peduncle segments of oil seed Brassica species: Influence of AgNO3 and silver thiosulphate. Plant Cell Tiss. Organ Cult., 51, 229–232.
Ercisli S., 2004. A short review of the fruit germplasm resources of Turkey. Genetic Res. Crop Evaluat., 51, 419–435.
Esitken A., Yildiz H.E., Ercisli S., Donmez M.F., Turan M., Gunes A., 2010. Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Sci. Hort., 124, 62–66.
Fiola J.A., Hassan M.A., Swartz, H.J., Bors R.H., McNicols R., 1990. Effect of thidiazuran, light influence rates and kanamycin on In vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant. Cell. Tiss. Org. Cult., 20, 223–228.
Hare P.D., Staden J., Van Staden J., 1994. Inhibitory effect of TDZ on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol., 35, 1121–1125.
Heinonen I.M., Meyer A.S., Frankel E.N., 1998. Antioxidant activity of berry phenolics on human low-density lipoprotein and liposome oxidation. J. Agric. Food Chem., 46, 4107–4112.
Huetteman C.A., Preece J.E., 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Organ Cult. 33, 105–119.
Kafkas E., Koşar M., Paydaş S., Kafkas S., Başer K.H.C., 2007. Quality characteristics of strawberry genotypes at different maturation stages. Food Chem., 100, 1229–1236.
Koşar M., Kafkas E., Paydaş S., Başer K.H.C., 2004. Phenolic composition of strawberry genotypes at different maturation stages. J. Agric. Food Chem., 52, 1586–1589.
Kumar V., Parvatam G., Aswathanarayana Ravishankar G., 2009. AgNO3 – a potential regulator of ethylene activity and plant growth modulator, Electronic J. Biotechnol., 12, 1–15.
Liu Z.R., Sanford J.C., 1988. Plant regeneration by organogenesis from strawberry leaf and runner culture. Hort Sci., 23, 1056–1059.
Llyod G., McCown B., 1980. Commercially feasible micropropogation of mountain laural (Kalmia latifolia) by use of shoot tip cultures. Comb. Proc. Int. Soc. 30, 421–427.
Malik K.A., Saxena P.K., 1992. Thidiazuron induces high frequency shoot regeneration in intact seedlings of pea, chickpea and lentil. Aust. J. Plant Physiol., 19, 731–740.
Mohan V., Gokulakrishnan K., Deepa R., Shanthirani C.S., Datta M., 2005. Association of physical inactivity with components of metabolic syndrome and coronary artery disease the Chennai Urban Population Study (CUPS no. 15). Diabet. Med., 22, 1206–1211.
Nehra N.S., Stushnoff C., 1989. Direct shoot regeneration from strawberry leaf disks. J. Amer. Soc. Hort. Sci., 114, 1014–1018.
Petri C., Burgos L., 2005. Transformation of fruit trees: useful breeding tool or continued future prospect? Trans. Res. 14, 15–26.
Sutter E.G. Ahmadi H., Labavitch J.M., Altman A., Ziv M., 1997. Direct regeneretion of strawberry (Fragaria × ananassa Duch.) from leaf disks. Acta Hortic., 447, 243–245.
Taji A., Kumar P.P., Lakshmanan P., 2002. In vitro plant breeding. New York: Food Products Pres.
Takashi H., Jaffe M.J., 1984. Further studies of auxin and ACC induced feminization in cucumber plant using ethylene inhibitors. Phyton, 44, 81–86.
Wang R.F., Cao W.W., Cerniglia C.E., 1996. PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl. Environm. Microbiol., 62, 1242–1247.
Żebrowska J.I., Hortyński J., 2002. Plant regeneration from leaf explants in strawberry (Fragaria × ananassa Duch.). Acta Hortic., 567, 313–315.
Zimmermann R.H. 1981. Micropropagation of fruit plants. Growth regulators in fruit production. Acta Hortic., 120, 217–227.


Duygu Ayvaz Sönmez 
Yaltır A.Ş., Sarıhuğlar mah., Adana, Turkey
Ebru Kafkas 
University of Çukurova, Balcalı, Adana, Turkey



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