THE EFFECT OF 6-BENZYLAMINOPURINE, THIDIAZURON AND THE TYPE OF EXPLANTS ON in vitro PROPAGATION OF Yucca elephantipes Regel
Danuta Kozak
University of Life Sciences in LublinAbstract
Yucca elephantipes is an important commercial ornamental pot plant, excellent for growing in flats, patios or winter gardens. Traditional vegetative propagation of the most decorative yuccas is complicated due to a very low rate of propagation, so in vitro culture is an alternative method for commercial propagation of these plants. The influence of BA (0.4, 2.2, 4.4, 11.1, 22.2 μM) and TDZ (0.5, 2.3, 4.5, 11.4, 22.7 μM) on shoot multiplication of Yucca elephantipes Regel on Murashige and Skoog (MS) medium was studied. Explants cultured on medium without growth substances were used as a control. The two types of explants used in the experiment: shoot tips and nodal segments of shoots, were obtained from aseptically grown shoot clusters. When comparing regeneration capability of 2 types of Yucca elephantipes explants, it was found that more newly formed shoots and roots were obtained from nodes. The highest formation of shoots was obtained
from nodes on MS medium supplemented with 4.5 μM TDZ or 11.1 and 22.2 μM BA (6.5, 6.0, 5.8, respectively). The shoots regenerated from nodes showed best elongation. On the control medium and on the media with the lowest level of BA or TDZ, their average length was 31.0–37.8 mm. The growth regulator-free medium and the media with a low level of BA were the most effective in inducing roots.
Keywords:
Yucca, BA, TDZ, shoot tips, nodesReferences
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University of Life Sciences in Lublin
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