DEVELOPMENT OF HIGHBUSH BLUEBERRY (Vaccinium corymbosum hort. non L.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF MELATONIN
Wojciech Litwińczuk
Rzeszów UniversityMagdalena Wadas-Boroń
Rzeszów UniversityAbstract
The influence of melatonin (MEL) in comparison with IAA, IBA (all at 5.71 μM), and auxin-free (0) medium on development of highbush blueberry (Vaccinium corymbosum hort. non L.) ‘Herbert’ in vitro shoot cultures was examined. Depending on the kind of hormone in vitro cultures consisted of various number of axillary (AX) and adventitious (AD) shoots. The influence of melatonin on in vitro cultures was intermediate but more similar to IAA than IBA. Production of axillary shoots on media supplemented with MEL and IAA was comparable and higher than on medium with IBA. In contrast to IAA melatonin reduced development of adventitious shoots. Contrary to IBA-obtained cultures AX shoots grown on ‘IAA’, ‘0’, and ‘MEL’ media resembled AD shoots.
Keywords:
micropropagation, axillary shoots, adventitious shoots, auxins, melatonin, highbush blueberryReferences
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11-17 August, 2002. Acta Hort. (2004), (629), 425–432.
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Posmyk M.M., Bałabusta M., Wieczorek M., Sliwinska E., Janas K.M., 2009. Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress. J. Pineal Res., 46 (2), 214–223.
Posmyk M.M., Janas K.M., 2009. Melatonin in plants. Acta Physiol. Plant. 31 (1), 1–11.
Read E.R., Wildung D.K., Hartley C.A., 1989. Field performance of in vitro-propagated ‘Northblue’ bluberries. Acta Hort., 241, 191–194.
Zimmerman R.H., Broome O.C., 1980. Blueberry micropropagation. Proceedings of the Conference on Nursery Production of Fruit Plants through Tissue Culture USDA-SEA, Agric. Res. Results ARR-NE., 11, 44–47.
Butkus V., Pliszka K., 1993. The highbush blueberry – a new cultivated species. Acta Hort. 346, 81–85.
Cohen D., Elliot D., 1979. Micropropagation methods for blueberries and tamarillos. Combined Proceedings, Int. Plant Propagators' Soc. 29, 177–179.
De Klerk G.J., 1990. How to measure somaclonal variation. Acta Bot. Neerlandica. 39 (2), 129–144.
Debnath S.C., McRae K.B., 2001. An efficient in vitro shoot propagation of cranberry (Vaccinium macrocarpon Ait.) by axillary bud proliferation. In vitro Cellular and Developmental Biology Plant 37 (2), 243–249.
El Shiekh A., Wildung D.K., Luby J.J., Sargent K.L., Read P.E., 1996. Long-term effects of propagation by tissue culture or softwood single-node cuttings on growth habit, yield, and berry weight of ‘Northblue’ blueberry. J. Am. Soc. Hort. Sci. 121 (2), 339–342.
Grout J.M., Read P.E., Wildung D.K., 1986. Influence of tissue culture and leaf-bud propagation on the growth habit of ‘Northblue’ blueberry. J. Am. Soc. Hort. Sci. 111 (3), 372–375.
Gustavsson B.A., 1999. Plant breeding and domestication of lingonberry (Vaccinium vitisidaea L.). Acta Univ. Agric. Sueciae Agr. 198 (23), 75.
Hernandez-Ruiz J., Cano A., Arnao M.B., 2004. Melatonin: a growth-stimulating compoud present in lupin tissues. Planta 220, 140–144.
Hosier M.A., Flatebo G., Read P., 1985. In Vitro Propagation of Lingonberry. HortScience 20 (3), 364–365.
Janas K. M., Szafrańska K., Posmyk M., 2005. Melatonina w roślinach (in Polish). Kosmos. Probl. Nauk Biol., 54, 251–258.
Kępczyńska E., Skoblińska B., Kępczyński J., 2004. Wpływ melatoniny na tworzenie się pędów przybyszowych róży miniaturowej White Gem w kulturach in vitro (in Polish). Biotechnologia (2)65, 155–161.
Litwińczuk W., 2001. Comparison of variability of chosen morphological traits of highbush blueberries (Vaccinium × corymbosum L.) obtained in vitro through axillary and adventitious shoots. Horticulture and Vegetable Growing. (Sodininkyste ir Darzininkyste), 20 (3), 45–50.
Litwińczuk W., Szczerba G., Wrona D., 2005. Field performance of highbush blueberries (Vaccinium × corymbosum L.) cv. ‘Herbert’ propagated by cuttings and tissue culture. Sci. Hort. 106, 162–169.
Litwińczuk W., Wadas M., 2008. Auxin-dependent development and habituation of highbush blueberry (Vaccinium × covilleanum But. et Pl.) ‘Herbert’ in vitro shoot cultures. Sci. Hort. 119, 41–48.
Lyrene P.M., Perry J.L., 1988. Blueberries (Vaccinium spp.). Biotechnol. Agric. Forestry, 6, 187–197.
Marcotrigiano M., McGlew S.P., 1991. A two-stage micropropagation system for cranberries. J. Am. Soc. Hort. Sci. 116 (5), 911–916.
Murch S.J., Simmons C.B., Saxena P.K., 1997. Melatonin in feverfew and other medicinal plants. The Lancet, 350, 1598–1599.
Murch S.J., Saxena P.K., 2002. Role of indoleamines in regulation of morphogenesis in in vitro cultures of St. John's wort (Hypericum perforatum L.). The future for medicinal and aromatic plants, a proceedings of the XXVI International Horticultural Congress, Toronto, Canada,
11-17 August, 2002. Acta Hort. (2004), (629), 425–432.
Orlikowska T., 1986. Micropropagation of highbush blueberry. Fruit Sci. Rep. 13 (3), 105–115.
Pliszka K., Rojek H., 1994. Porównanie trzech odmian borówki wysokiej rozmnażanych in vitro i tradycyjnie (in Polish). Materiały 33 Ogólnopolskiej Konferencji Sadowniczej ISiK – Skierniewice, 280–282.
Posmyk M.M., Bałabusta M., Wieczorek M., Sliwinska E., Janas K.M., 2009. Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress. J. Pineal Res., 46 (2), 214–223.
Posmyk M.M., Janas K.M., 2009. Melatonin in plants. Acta Physiol. Plant. 31 (1), 1–11.
Read E.R., Wildung D.K., Hartley C.A., 1989. Field performance of in vitro-propagated ‘Northblue’ bluberries. Acta Hort., 241, 191–194.
Zimmerman R.H., Broome O.C., 1980. Blueberry micropropagation. Proceedings of the Conference on Nursery Production of Fruit Plants through Tissue Culture USDA-SEA, Agric. Res. Results ARR-NE., 11, 44–47.
Wojciech Litwińczuk
Rzeszów University
Rzeszów University
Magdalena Wadas-Boroń
Rzeszów University
Rzeszów University
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