Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 20 No. 5 (2021)

Articles

The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

DOI: https://doi.org/10.24326/2021.5.12
Submitted: April 9, 2021
Published: 2021-10-29

Abstract

Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth.

References

  1. Ahmed, M., Hafiz, I., Abbasi, N., Ejaz, S., Anjum, M. (2015). Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. J. Intern. Sci. Vigne Vin, 49(1), 37–45. https://doi.org/10.20870/oeno-one.2015.49.1.95
  2. Al Malki, A.A.H.S., Elmeer, K.M.S. (2010). Influence of auxin and cytokinine on in vitro multiplication of Ficus anastasia. Afr. J. Biotechnol., 9(5), 635–639. https://doi.org/10.5897/AJB09.1168
  3. Aslam, J., Mujib, A., Sharma, M.P. (2013). In vitro propagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant. Saudi J. Biol. Sci., 20(1), 63–68. https://doi.org/10.1016/j.sjbs.2012.11.005
  4. Biondo, R., Soares, A.M., Bertoni, B.W., Franca, S.C., Pereira, A.M.S. (2004). Direct organogenesis of Mandevilla illustris (Vell) Woodson and effect of its aquaeous extract on the enzymatic and toxic activities of Crotalus durissus terrificus snake venom. Plant Cell Rep., 22, 549–552. https://doi.org/10.1007/s00299-003-0722-6
  5. Biondo, R., Souza, A.V. Bertoni, B.W., Soares, A.M., Franca, S.C., Pereira, A.M.S. (2007). Micropropagation, seed production and germplasm bank of Mandevilla velutina (Mart.) Woodson. Sci. Agric., 64(3), 263–268. https://doi.org/10.1590/S0103-90162007000300008
  6. Capeletti, R., Sabadini, S., Mezzetti, B. (2016). The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Sci. Hortic., 207, 117–204. https://doi.org/10.1016/j.scientia.2016.05.016
  7. Chuenboonngarm, N., Charoonsote, S., Bhamarapravati, S. (2001). Effect of BA and 2iP on shoot proliferationand and somaclonal variation of Gardenia jasminoides Ellis in vitro culture. ScienceAsia, 27, 137–141. https://doi.org/10.2306/scienceasia1513-1874.2001.27.137
  8. Cordeiro, S.Z., Simas, N.K., Henriques, A.B., Lage, C.L.S., Sato, A. (2012). Micropropagation of Mandevilla moricandiana (A.D.C.) Woodson. In Vitro Cell. Dev. Biol.-Plant, 48, 620–626.
  9. Cordeiro, S.Z., Simas, N.K., Henriques, A.B., Sato, A. (2014). Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil. Crop Breed. Appl. Biotechnol., 14, 2. https://doi.org/10.1590/1984-70332014v14n2a19
  10. Dai, W., Magnusson, V.A., Hatterman-Valenti, H., Carter, J.F. (2006). Micropropagation of ‘Amethyst’ purple raspberry (Rubus occidentalis L. × R. idaeus L. ‘Amethyst’). J. Environ. Hort., 24(1), 35–38.
  11. Garcia-Luis, A., Molina, R.V., Varona, V., Castello, S., Guardiola, J.L. (2006). The influence of explant orientation and contact with the medium on the pathway of shoot regeneration in vitro in epicotyl cuttings of Troyer citrange. Plant Cell, Tiss. Organ Cult., 85, 137–144. https://doi.org/10.1007/s11240-005-9060-4
  12. Gomez-Leyva, J.F., Acosta, L.A.M., Muraira, I.G.L., Espino, H.S., Ramirez-Cervantes, F., Andrade-Gonzalez, I. (2008). Multiple shoot regeneration of roselle (Hibiscus sabdariffa L.) from shoot apex culture system. Int. J. Bot., 4(3), 326–330.
  13. Gonbad, R.Z., Sinniah, U.M., Aziz, M.A., Mohamad, R. (2014). Influence of cytokinins in combination with GA3 on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze). Sci. World J., Article ID 943054. https://doi.org/10.1155/2014/943054
  14. Gulati, A., Jaiwal, P.K. (1992). In vitro induction of multiple shoots and plant regeneration from shoot tips of mung bean (Vigna radiata (L.) Wilczek). Plant Cell Tiss. Organ Cult., 29, 199–205. https://doi.org/10.1007/BF00034353
  15. Jesionek, A., Kokotkiewicz, A., Wlodarska, P., Filipowicz, N., Bogdan, A., Ochocka, R., Szreniawa-Sztajnert, A, Zabiegała, B., Bucinski, A., Luczkiewicz, M. (2016). In vitro propagation of Rhododendron tomentosum – an endangered essential oil bearing plant from peatland. Acta Biol. Crac., Ser. Bot., 58(2), 29–43. https://doi.org/10.1515/abcsb-2016-0019
  16. Kaviani, B., Zamiraee, F., Zanjani, S.B., Tarang, A., Torkashvand, A.M. (2014). In vitro flowering and micropropagation of lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA) Acta Sci. Pol., Hortorum Cultus, 13(4), 145–155.
  17. Kim, S.-H., Kim, S-K. (2002). Effect of cytokinins on in vitro growth of grapes (Vitis spp.). J. Plant Biotech., 29(2), 123–127.
  18. Kokotkiewicz, A., Łuczkiewicz, M., Hering, A., Ochocka, R., Goryński, K., Bucinski, A., Sowiński, P. (2012). Micropropagation of Cyclopia genistoides, an endemic South African plant of economic importance. Z. Nat.forsch., C J. Biosci., 67(1–2), 65–76. https://doi.org/10.1515/znc-2012-1-209
  19. Kozak, D., Parzymies, M., Świstowska, A., Marcinek, B. (2019). The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro. Acta Sci. Pol. Hortorum Cultus, 18(4), 111–119. https://doi.org/10.24326/asphc.2019.4.10
  20. Liao, Y-K., Chuang, M-Ch. (2014). Micropropagation of Quercus aliena Blume var. aliena from explants of mature trees. Taiwan J. For Sci., 29(2), 117–131.
  21. Mackay, W.A., Kitto, S.L. (1988). Factors affecting in vitro shoot proliferation of French tarragon. J. Am. Soc. Hort. Sci., 113, 282–287.
  22. Mastuti, R., Munawarti, A, Firdiana, E.R. (2017). The combination effect of auxin and cytokinin on in vitro callus formation of Physalis angulata L. – AIP Conference Proceedings 1908, 040007. https://doi.org/10.1063/1.5012721
  23. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant., 15, 473–479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  24. Mahmood, S., Hauser, B. (2015). Influence of cytokinins on the shoot proliferation and subsequent rooting in rose. Asian J. Agric. Res., 9(5), 259–267. https://doi.org/10.3923/ajar.2015.259.267.
  25. Murai, Y., Harada, H., Yamashita, H. (1997). In vitro propagation of apricot (Prunus armeniaca L.) cv. ‘Bakuoh junkyou’. J. Jpn. Soc. Hortic. Sci., 66, 475–480. https://doi.org/10.2503/JJSHS.66.475
  26. Mustafa, N.S., Taha, R.A. (2012). Influence of plant growth regulators and subculturing on in vitro multiplication of some fig (Ficus carica) cultivars. J. Appl. Sci. Res., 8(8), 4038–4044.
  27. Nair, L.G., Seeni, S. (2001). Rapid in vitro multiplication and restoration of Celastrus paniculatus Willd. sub sp. paniculatus (Celastraceae), a medicinal woody climber. Indian J. Exp. Biol., 39, 697–704.
  28. Niranjan, M.H., Sudarshana, M.S., Girisha, S.T. (2010). In vitro multiple shoot induction from excised shoot tips and nodal segment explants of – Lagerstroemia indica L. – a medicinal cum ornamental shrub. J. Biomed. Sci. Res., 2(3), 212–217.
  29. Palla, K.J., Pijut, P.M. (2011). Regeneration of plants from Fraxinus americana hypocotyls and cotyledons In Vitro Cell. Dev. Biol. Plant., 47(2), 250–256. https://doi.org/10.1007/s11627-011-9360-9
  30. Pawłowska, B. (2011). The effect of BA and GA3 on the shoot multiplication of in vitro cultures of Polish wild roses. Folia Hort., 23(2), 145–149. https://doi.org/10.2478/v10245-011-0022-5
  31. Ponchia, G., Gardiman, M. (1993). The micropropagation and post-acclimation growth of Prunus laurocerasus L. cv. Otto Luyken. Adv. Hort. Sci., 7(1), 11–14.
  32. Ružić, Dj.V., Vujović, T.I. (2008). The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). Hort. Sci. (Prague), 35(1), 12–21.
  33. Saini, H.K., Gill, M.S., Gill, M.I.S. (2010). Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lush.). Indian J. Biotech., 9, 419–423.
  34. Sakhanokho, H.F. (2008). In vitro multiple shoot induction and plant regeneration from shoot apices of Hibiscus acetosella Welw. ex. Hiern. J. Crop Improv., 21(2), 201–208. https://doi.org/10.1080/15427520701885774
  35. Sharma, S., Shahzad, A., Anis, M. (2010). In vitro shoot organogenesis and regeneration of plantlets from nodal explants of Murraya koenigii (L.) Spreng. (Rutaceae), a multipurpose aromatic medicinal plant. Med. Aromat. Plant Sci. Biotechnol., 4(1), 33–36.
  36. Silva da, G.M., Alvarenga, J.P., Bertolucci, S.K.V., Tavares, M.C., Rodrigues, M.A., Pinto, J.E.B.P. (2017). Effect of chemical and physical factors in in vitro propagation and volatile fraction analysis of Aloysia triphylla (L’Herit) Britton. Acta Hortic., 1155, 309–316. https://doi.org/10.17660/ActaHortic.2017.1155.44
  37. Soh, W-Y., Choi, P-S., Cho, D-Y. (1998). Effects of cytokinin on adventitious root formation in callus cultures of Vigna unguiculata (L.) walp. In vitro Cell. Dev. Biol.--Plant, 34, 189–195. https:/doi.org/10.1007/BF02822707
  38. Sudha, C.G., Seeni S. (1996). In vitro propagation of Rauwolfia micrantha, a rare medicinal plant. Plant Cell Tiss. Organ Cult., 44, 243–248.
  39. Taha, R.A., Hassan, S.A.M., Ahmed, D.M.M., Zaied, M.S. (2016). A comparative study on different cytokinins types and carbon source concentrations on in vitro proliferation of jojoba (Simmondsia chinensis Link (Schneider)). Inter. J. Chem. Tech. Research, 9(8), 178-184.
  40. Thomsone, S., Gertnere, D. (2003). In vitro shoot regeneration from flower and leaf explants in Rhododendron. Biol. Plant., 46(3), 463–465.
  41. Vengadesan, G., Ganapathi, A., Anand, R.P., Anbazhagan, V.R. (2002). In vitro propagation of Acacia sinuata (Lour.) Merr. via cotyledonary nodes. Agrofor. Syst., 55(1), 9–15.
  42. Wojtania, A., Gabryszewska, E., Woźniak, P. (2006). Zastosowanie kultur in vitro w rozmnażaniu Dipladenia sanderi Hemsl. [The use of in vitro cultures in propagation of Dipladenia sanderi Hemsl.], Zesz. Probl. Postęp. Nauk Rol., 510(2), 717–722.
  43. Yonghong, L., Daxing, Z., Lijuan, X. (2004). Studies of the micropropagation of Mandevilla sanderi. J. For. Sci. Technol., 29(5), 49–51.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 3 4 5 > >> 

Similar Articles

1 2 3 4 5 6 7 8 > >> 

You may also start an advanced similarity search for this article.