MORPHOLOGICAL CHARACTERISTICS AND PATHOGENICITY OF Diaporthe eres ISOLATES TO THE FRUIT TREE SHOOTS
Barbara Anna AbramczykDepartment of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation – State Research Institute, Puławy
Ewa Dorota KrólDepartment of Plant Protection, University of Life Sciences in Lublin
Ewa Dorota ZalewskaDepartment of Plant Protection, University of Life Sciences in Lublin
Beata ZimowskaDepartment of Plant Protection, University of Life Sciences in Lublin
This work is a continuation of research on Diaporthe genus isolates obtained in 2010−2012 from fruit trees in Poland, which on the basis of previously conducted molecular tests, have been identified as one species belonging to the Diaporthe eres species complex. The aim of this study was to determine the morphology and pathogenic abilities of tested Diaporthe eres isolates. The experiment included cross tests, in which the shoots of apple, pear, cherry and plum trees were inoculated with each of the 4 isolates derived from each mentioned host plants. As a result of experiment, the pathogenic nature of D. eres in relation to the shoots of fruit trees, was confirmed. The isolates were also characterized on the basis of the colony appearance and spore dimensions. Morphological features of studied D. eres cultures were very similar, regardless of the isolate and the host plant, from which they were obtained. All tested isolates formed alpha and beta conidia having the same range size.
Keywords:macroscopic features, microscopic features, orchard plants, pathogenic ability, Phomopsis
Abramczyk, B., Król, E. (2016). Use of RAPD-PCR and ITS markers for identification of Diaporthe/Phomopsis from fruit trees in south-eastern Poland. Acta Sci. Pol. Hortorum Cultus, 15(6), 161–175.
Baumgartner, K., Fujiyoshi, P.T., Travadon, R., Castlebury, L.A., Wilcox, W.F., Rolshausen, P.E. (2013). Characterization of species of Diaporthe from wood cankers of grape in eastern North American vineyards. Plant Dis., 97, 912–920.
Cinelli, T., Mondello, V., Marchi, G., Burruano, S., Alves, A., Mugnai, L. (2016). First report of Diaporthe eres associated with cane blight of grapevine (Vitis vinifera) in Italy. Plant Dis., 100(2), 532–532.
Crous, P.W. (2005). Impact of molecular phylogenetics on the taxonomy and diagnostics of fungi. Bull OEPP/EPPO, 35, 47–51.
Diogo, E.L.F., Santos, J.M., Phillips, A.J.L. (2010). Phylogeny, morphology and pathogenicity of Diaporthe and Phomopsis species on almond in Portugal. Fungal Divers., 44, 107–115.
Dissanayake, A.J, Zhang, W., Liu, M., Hyde, K.D., Zhao, W., Li, X.H., Yan J.Y. (2017). Diaporthe species associated with peach tree dieback in Hubei, China. Mycosphere, 8(5), 533–549. DOI: 10.5943/mycosphere/8/5/3.
Du, Z., Fan, X.L., Hyde, K. D., Yang, Q., Liang, Y.|M, Tian, Ch. M. (2016). Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China. Phytotaxa, 269(2), 090–102.
Farr, D.F., Castlebury L.A., Pardo-Schultheiss R.A. (1999). Phomopsis amygdali causes peach shoot blight of cultivated peach trees in the southeastern United States. Mycologia, 91, 1008–1015.
Farr, D.F., Castlebury, L.A., Rossman, A.Y. (2002). Morphological and molecular characterization of Phomopsis vaccinii and additional isolates of Phomopsis from blueberry and cranberry in the eastern United States. Mycologia, 94, 494–504.
Gomes, R.R, Glienke, C., Videira, S.I.R., Lombard, L., Groenewald, J.Z., Crous, P.W. (2013). Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia, 31, 1–41.
Guarnaccia, V., Crous, P.W. (2017). Emerging citrus diseases in Europe caused by species of Diaporthe. IMA Fungus, 8(2), 317–334. DOI:10.5598/imafungus.2017.08.02.07.
Guarnaccia, V., Groenewald, J.Z., Woodhall, J., Armengol, J., Cinelli, T., Eichmeier, A., Ezra, D., Fontaine, F., Gramaje, D., Gutierrez-Aguirregabiria, A., Kaliterna, J., Kiss, L., Larignon, P., Luque, J., Mungai, L., Naor, V., Raposo, R., Sándor, E., Váczy, K.Z., Crous, P.W. (2018). Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe. Persoonia, 40, 135–153.
Kaliterna, J., Miličević, T., Cvjetković, B. (2012). Grapevine trunk diseases associated with fungi from the Diaporthaceae family in Croatian vineyards. Arh. Hig. Rada Toksikol., 63, 471–479. DOI: 10.2478/10004-1254-63-2012-2226.
Karaoglanidis, G.S., Bardas, G. (2006). First report of Phomopsis fruit decay on apple caused by Phomopsis mali in Greece. Plant Dis., 90(3), 375.
Kirk, P.M., Cannon, P.M., Minter, D.W., Stalpens, J.A. (2008). Ainsworth & Bisby’s Dictionary of Fungi. 10th ed. CAB International, 640 pp.
Król, E. (2002). Determination of genetic variability within Phomosis spp. using RAPD method. Phytopathol. Pol., 25, 35–46.
Król, E. (2005a) Influence of some chemicals on the viability of Phomopsis viticola Sacc. spores. J. Plant Prot. Res., 45(3), 195–204.
Król, E. (2005b). Identification and differentiation of Phomopsis spp. isolates from grapevine and some other plant species. Phytopathol. Pol., 35, 151–156.
Król, E. (2006). Grzyby zasiedlające zdrowe łozy winorośli (Vitis spp.) w wybranych szkółkach. Acta Agrobot., 59(2), 163–173.
Król, E. (2007). Phomopsis viticola Sacc. jako pato-gen winorośli na świecie i w Polsce. Post. Nauk Roln., 4, 85–96.
Król, E., Kowalik, B. (2010). Charakterystyka grzybów rodzaju Phomopsis wyizolowanych z roślin sadowniczych. Zesz. Probl. Post. Nauk Roln./Prog. Plant Prot. Res., 554, 53–59.
Król, E., Kowalik, B. (2011). Grzyby z rodzaju Phomopsis występujące na pędach roślin sadowniczych. Post. Nauk Roln., 2, 31–42.
Luongo, L., Santori, L., Riccioni, L. (2011). Phomopsis sp. associated with postharvest fruit rot of kiwifruit in Italy. J. Plant Pathol., 93(1), 205–209.
Machowicz-Stefaniak, Z. (1993). Phomopsis viticola Sacc. (Sphaeropsidales Deuteromycotina) nowy w Polsce patogen pędów winorośli. Acta Mycol., 28, 157–160.
Michalecka, M., Bryk, H., Seliga, P. (2016). Identification and characterization of Diaporthe vaccinii Shear causing upright dieback and viscid rot of cranberry in Poland. Eur. J. Plant Pathol. DOI: 10.1007/s10658-016-1114-4.
Mostert, L, Crous, P.W., Kang, C.J., Phillips, A.J.L. (2001). Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia, 93, 145–166.
Rehner, S.A., Uecker, F.A. (1994). Nuclear ribosomal internal transcribed spacer phylogeny and host diversity in the coelomycete Phomopsis. Can. J. Bot., 72, 1666–1674.
Rossman, A.Y., Udayanga, D., Castelbury, L.A., Hyde, K.D. (2014). (2304) Proposal to conserve the name Diaporthe eres against twenty-one competing names (Ascomycota, Diaporthales, Diaporthaceae). Taxon, 63, 934–935.
Rossman, A.Y., Adams, G.C., Cannon, P.F., Castlebury, L.A., Crous, P.W. et al. (2015) Recommendations of generic names in Diaporthales competing for protection or use. IMA Fungu, 6, 145–154.
Santos, J.M., Phillips, A.J.L. (2009). Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers., 34, 111–125.
Schilder, A.M.C., Erincik, O., Castlebury, L., Rossman, A., Ellis, M.A. (2005). Characterization of Phomopsis spp. infecting grapevines in the Great Lakes region of North America. Plant Disease, 89, 755–762.
Sutton, B.C. (1980). The Coelomycetes, fungi imperfecti with picnidia, acervuli and stroma. Commonwealth Mycological Institute, Kew, Surrey.
Udayanga, D., Liu, X.X., McKenzie, E.H.C., Chukeatirote, E., Bahkali, A.H., Hyde, K.D. (2011). The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Divers., 50, 189–225.
Udayanga, D., Castlebury, L.A., Rossman, A.Y., Chukeatirote, E., Hyde, K.D. (2014a). Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex. Fungal Divers., 67, 203–229.
Udayanga, D., Castlebury, L.A., Rossman, A.Y., Hyde, K.D. (2014b). Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis. Persoonia, 32, 83–101.
Uddin, W., Stevenson, K.L., Pardo-Schultheiss, R.A. (1997). Pathogenicity of a species of Phomopsis causing a shoot blight on peach in Georgia and evaluation of possible infection courts. Plant Dis., 81, 983–989.
Uddin, W., Stevenson, K.L., Pardo-Schultheiss, R.A., Rehner, S.A. (1998). Pathogenic and molecular characterization of three Phomopsis isolates from peach, plum and Asian pear. Plant Dis., 82, 732–737.
Uecker, F.A. (1988). A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycol. Mem., 13, 231 pp.
Van Niekerk, J.M., Groenewald, J.Z., Farr, D.F., Fourie, P.H., Hallen, F., Crous, P.W. (2005). Reassessment of Phomopsis species on grapevines. Australas. Plant Pathol., 34, 27–39.
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