COMPARISON OF in vitro LIPOXYGENASE, XANTHINE OXIDASE INHIBITORY AND ANTIOXIDANT ACTIVITY OF Arnica montana AND Arnica chamissonis TINCTURES
Urszula Gawlik-Dziki
University of Life Sciences in LublinMichał Świeca
University of Life Sciences in LublinDanuta Sugier
University of Life Sciences in LublinJoanna Cichocka
University of Life Sciences in LublinAbstrakt
Preparations of Arnica have been used for treatment of post-trauma effects and inflammatory diseases. The aim of the study was to determine and compare the antioxidant activities and in vitro lipoxygenase (LOX) and xanthine oxidase (XO) inhibitory activity of different parts of A. montana (AM) and A. chamissonis (AC) with reference to the polyphenolic compounds content. Based on IC50 values could be concluded that active compounds of AM acted mainly as a XO inhibitors while LOX was more effective inhibited by AC samples. The highest antiradical activity was observed in the cases of AM flower heads and AC herbs tincture. All studied materials exhibited high chelating power (with IC50 ranged from 10.96 to 11.84 mg/ml) and ability to inhibition of lipid peroxidation (IC50 ranged from 10.00 to 15.09 mg/ml). Arnica tinctures possess high antioxidant abilities and XO and LOX inhibitory activity that might be helpful in preventing or slowing the progress of free radical dependent diseases. Additionally, results clearly showed that A. chamissonis might be a valuable herbal raw material.
Słowa kluczowe:
arnica, inflammation, enzyme inhibition, phenolics, antioxidantsBibliografia
Bahorun T., Luximon-Ramma A., Crozier A., Aruoma O.I., 2004. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J. Sci. Food Agric. 84, 1553–1561.
Brand-Williams W., Cuvelier E., Berset C.M., 1995. Use of free radical method to evaluate antioxidant activity. LWT. 28, 25–30.
Cassels A.C., Walsh C., Belin M., Cambornac M., Robin J.R., Lubrano C., 1999. Establishment of a plantation from micropropagated Arnica chamissonis a pharmaceutical substitute for the endangered A. montana. Plant Cell Tiss. Org. Cult. 56, 139–144.
Erel O., 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37, 277–285.
Ferraz Filha Z.S., Vitolo I.F., Fietto L.G., Lombardi J.A., Saude-Guimaraes D.A., 2006. Xanthine oxidase inhibitory activity of Lychnophora species from Brazil (“Arnica”). J. Ethnopharmacol. 107, 79–82.
Gawlik-Dziki U., Dziki D., Baraniak B., Lin R., 2009. The effect of simulated digestion in vitro on bioactivity of wheat bread with Tartary buckwheat flavones addition, LWT. 42, 137–143.
Guo J.-T., Lee H.-L., Chiang S.-H., Lin H.-I., Chang C.-Y., 2001. Antioxidant properties of the extracts from different parts of broccoli in Taiwan. J. Food Drug Anal. 9, 96–101.
Heilmann J., Merfort I., Weiss M., 1995. Radical scavenger activities of different 3`,4`-duhydrixyflavonols and 1,5-dicaffeoylquinic acid studied by inhibition of chemiluminescence. Planta Med. 61, 435–38.
Hollman P.C.H., Katan M.B.,1997. Absorption, metabolism and health effects of dietary flavonoids in man. Biomed. Pharmacother. 51,305–310.
Juntachote T., Berghofer E., 2005. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chem. 92, 193–202.
Kaur G., Alam M.S., Jabbar Z., Javed K., Athar M., 2006. Evaluation of antioxidant activity of Cassia siamea flowers. J. Ethnopharmacol. 108, 340–348.
Kuo J.-M., Yeh D.-B., Pan B., 1999. Rapid Photometric Assay Evaluating Antioxidative Activity in Edible Part Material. J. Agric. Food Chem. 47, 3206–3209.
Liang M.C., Bardhan S., Pace E.A., Rosman D., Beutler J.A., Porco J.A. Jr, Gilmore T.D., 2006. Inhibition of transcription factor NF-kappaB signaling proteins IKKbeta and p 65 through specific cysteine residues by epoxyquinone A monomer: correlation with its anti-cancer cell growth activity. Biochem. Pharmacol. 71(5), 634–45.
Murota K., Terao J., 2003. Antioxidative flavonoid quercetin: implications of its intestinal absorption and metabolism. Arch. Biochem. Biophys. 417, 12–17.
Oktay M., Gulcin I., Kufrevioglu, O. I., 2003. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT. 36, 263–271.
Oyaizu M., 1986. Studies on products of browning reaction – Antioxidative activities of products of browning reaction prepared from glucosamine. Japan. J. Nutr. 44, 307–315.
Polish Pharmacopoeia VI., 1999. Warszawa PTF, 390–392.
Sherwin E.R., 1990. Antioxidants. In: Food Additives, eds Branen, A.L., Davidson, P.M., & Salminen, S. Marcel Dekker Inc, New York, USA, 144–145.
Singleton V.L., Rossi J.A., 1965. Colorimetry of total phenolics witch phosphomolybdicphodphotungstics acid reagents. Am. J. Enol. Vitic. 16, 144–158.
Spitaler R., Winkler A., Lins I., Yanar S., Stuppner H., Zidorn C., 2008. Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3 year comparison. J. Chem. Ecol. 34, 369–75.
Sweeney A., Wyllie S.G., Shalliker R. A., Markhan J.L., 2001. Xanthine oxidase inhibitory activity of selected Australian native plants. J. Ethnopharmacol. 75, 273–277.
Szaufer-Hajdrych M., 2004. Phenolic acids in leaves of species of the Aquilegia genus. Herba Pol. 50, 10–14.
Wagner S., Merfort I., 2007. Skin penetration behaviour of sesquiterpene lactones from different Arnica preparations using a validated GC-MSD method. J. Pharm. Biomed. Anal. 43, 32–38.
Zhao G.-R., Xiang Z.-J., Ye T.-X., Yuan Y.-J., Guo Z.-X., 2006. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem. 99, 767–774.
University of Life Sciences in Lublin
University of Life Sciences in Lublin
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Licencja
Artykuły są udostępniane na zasadach CC BY 4.0 (do 2021 r. na zasadach CC BY-NC-ND 4.0 międzynarodowe).
Przysłanie artykułu do redakcji oznacza, że nie był on opublikowany wcześniej i nie jest rozpatrywany do publikacji gdzie indziej.
Autor podpisuje oświadczenie o oryginalności dzieła, wkładzie poszczególnych osób i źródle finansowania.