Preliminary studies of polymorphism of selected microsatellite sequences in different colour types of raccoon dog (Nyctereutes Procyonoides)
Andrzej Jakubczak
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinGrażyna Jeżewska-Witkowska
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinBeata Horecka
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinMonika Żelazowska
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinEleonora Kopeć
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinZofia Rupeć
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 LublinAbstrakt
Primary sequences designed for microsatellite loci in the domestic dog genome (FH2054, CPH3, FH2168, FH2164, FH2097), which were used in this study, also allowed for the
amplification of the corresponding loci in white and standard Raccoon dogs. In these groups the frequency of alleles, observed and expected heterozygosity, the polymorphic information content and the probability of parentage exclusion were analyzed. The studies allowed to define the expected degree of polymorphism of five microsatellite loci in the two color varieties of Raccoon dogs. All of the five selected microsatellite sequences may be used for checking the origin of Raccoon dogs and the construction of genetic maps within the canids. The obtained values of genetic similarity and genetic distance shows that there is a high genetic similarity between the groups of white and standard Raccoon dog genetic, and thus the small genetic distance. This suggests only small genetic differences between white and standard Raccoon dogs.
Słowa kluczowe:
Raccoon dog, microsatellite, PCR-multiplexBibliografia
359–362.
Fredholm M., Winterø A. K., 1995. Variation of short tandem repeats within and between species belonging to the Canidae family. Mamm. Genome, 6, 11–18.
Jakubczak A., Jeżewska G., 2008. Validation of StockMarks Set for identifying origin of species from the canine family. Med. Wet., 64 (6), 832–835.
Jakubczak A., Bugno-Poniewierska M., Jeżewska-Witkowska G., Horecka B., Buś-Kicman M., 2011. Genetic differentiation of intergeneric hybrids of Blue Frost foxes and their original
forms based on microsatellite polymorphism. Ann. Anim. Sci., 11, 2, 217–225.
Nei M., 1978. Estimation of average heterozygosity and genetic distance from a small numbers of individual.Genetics, 89, 583–590.
Radko A., 2008. Ocena polimorfizmu markerów mikrosatelitarnych DNA wykorzystywanych w kontroli rodowodów bydła w Polsce. Wiad. Zoot., 46, 4, 3–8.
Stępniak E., Zagalska M. M., Świtoński M., 2002. Use of RAPD technique in evolution studies of four species in the family Canidae. J. App. Genet., 43 (4), 489–499.
Ślaska B., Zięba G., Jeżewska G., 2007. Raccoon dog (Nyctereutesprocyonoides gray) parentage testing based on STR markers. EJPAU, 10 (3), 16.
Ślaska B., Zięba G., Rozempolska-Rucińska I., Jeżewska-Witkowska G., Jakubczak A., 2010. Evaluation of genetic biodiversity in farm-bred and wild Raccoon dogs in Poland. Folia Biol.
(Kraków), 58, 3–4, 195–199.
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
University of Life Sciences in Lublin, Department of Biological Basis of Animal Production, Akademicka 13, 20-950 Lublin
Licencja
Od 2022 r. artykuły są udostępniane na zasadach licencji Creative Commons uznanie autorstwa 4.0 międzynarodowa (CC BY 4.0). Artykuły opublikowane przed 2022 r. są dostępne na zasadach licencji Creative Commons uznanie autorstwa – użycie niekomercyjne – bez utworów zależnych 4.0 międzynarodowa (CC BY-NC-ND 4.0).
Przysłanie artykułu do redakcji oznacza, że nie był on opublikowany wcześniej, nie jest rozpatrywany do publikacji w innych wydawnictwach.
Autor podpisuje oświadczenie o oryginalności dzieła i wkładzie poszczególnych osób.