Abstract
Recently the interest in borage as a vegetable and medicinal plant has increased, yet the knowledge about a content of biologically active compounds in borage grown in Poland remains very scanty. In the experiment carried out in the south-eastern part of the country, fresh borage flowers and leaves contained 0.02%
and 0.12% of lipids with the highest level of palmitic acid, i.e. 44.5% and 33.4%, respectively. Flower lipids contained stearic, oleic, elaidic, linoleic, linolelaidic, arachidonic, myristic and lauric acid as well. Besides, leaf lipids were rich in α-linolenic acid (17.7%), less in palmitoleic acid with absence of arachidonic acid. Leaves were found to have 0.16% of essential oil, 1.0% of flavonoids, 9.2 mg vitamin C·100 g f.w.–1, 1.9 mg carotenoids·100 g f.w.–1, 0.77 mg chlorophyll a·1 g f.w.–1 and 0.22 mg chlorophyll b·1 g f.w.–1. Flowers contained more essential oil and vitamin C while less carotenoids, chlorophyll a and b and flavonoids. 45 components were found in the flower essential oil and among them 16 were identified with cumene (62.9%) as a major one. The content of other components was below 3%. As for the leaf essential oil, 18 compounds were detected, and 5 were identified with the highest level of cumene (58.5%) and hexenyl (13.3%). Generally, flowers had more polyphenols and their ferric reducing ability was higher than that of leaves. Besides, a content of remaining unreduced DPPH radical was higher in the flowers. Notably, the time necessary for 50% reduction of the initial concentration of DPPH radical was 2.5 times longer in flowers, whereas their antiradical efficiency 3 fold lower compared to leaves.
References
Adams, R.P. (2004). Identification of essential oil compounds by gas chromatography/quadrupole mass spectroscopy. Allured Pub. Corp., Carol Stream.
Aliakbarlu, J., Tajik, H. (2012). Antioxidant and antibacterial activities of various extracts of Borage officinalis flowers. J. Food Process. Preserv., 36, 539–544.
Al-Nowaihi, A.S., Khalifa, S.F., Hamed, K.A. (1987). A contribution to the taxonomy of Boraginaceae. Phytologia, 62(2), 107–125.
AOAC, Official Methods of Analysis of the AOAC 963.22 (2000). Methyl esters of fatty acids in oils and fats. 17th ed. Arlington-Virginia.
AOAC, Official Methods of Analysis of the AOAC 969.33 (2000). Fatty acids in oils and fats. 17th ed. Arlington-Virginia.
Asadi-Samani, M., Bahmani M., Rafieian-Kopaei M. (2014). The chemical composition, botanical characteristic and biological activities of Borago officinalis: a review. Asian Pac. J. Trop. Med., Suppl. 1, 22–28. DOI: 10.1016/S1995-7645(14)60199-1.
Bandoniene, D., Murkovic, M. (2002). The detection of radical scavenging compounds in crude extract of borage (Borago officinalis L.) by using an on-line HPLCDPPH
method. J. Biochem. Biophys. Methods, 53, 45–49.
Bandoniene, D., Venskutonis, P.R., Gruzdiene, D., Murkovic, M. (2002). Antioxidative activity of sage (Salvia officinalis L.), savory (Satureja hortensis L.) and borage
(Borago officinalis L.) extracts in rapeseed oil. Eur. J. Lipid Sci. Technol., 104, 286–292.
Beaubaire, N.A., Simon, J.E. (1987). Production potential of Borago officinalis L. Acta Hortic., 208, 101–103.
Benzie, I.F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: The FRAP Assay. Anal. Biochem., 239, 70–76.
Berti, M., Joublan, J.P., Serri, H., Gonzalez, M. (1998). Determination of harvest timeliness in borage. Cienc. Investig. Agrar. (Chile), 25, 119–126.
Bianco, V.V, Santamaria, P., Elia, A. (1998). Nutritional value and nitrate content in edible wild species used in southern Italy. Acta Hortic., 467, 71–87.
Biscotti, N., Pieroni, A. (2015). The hidden Mediterranean diet: wild vegetables traditionally gathered and consumed in the Gargano area, Apulia, SE Italy. Acta Soc.
Bot. Pol., 84(3), 327–338.
Borowy, A., Kiczorowski, P., Wójcik, I. (2016). Evaluation of fluazifop-P-butyl and napropamide usefulness for weed control in borage (Borago officinalis L.) cultivation.
Annales UMCS, sec. EEE, Horticulturae, 26(1), 1–12.
Brand-Williams, W., Cuvelier, M.E., Berest, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensm.-Wiss. Technol., 28, 25–30.
Charłampowicz, Z. (1966). Analizy przetworów z owoców, warzyw i grzybów. WPLS, Warszawa.
de Haro, A., del Río, M., Alcaide, B., Rapoport, H., Cabrera, A. (2004). Characterization and Evaluation of Species of the Boraginaceae Family as Source of
Gamma-Linolenic Acid for Mediterranean Conditions. Acta Hortic., 629, 231–237.
del Río, M., Fernández-Martínez, J., de Haro, A. (1993). Wild and cultivated Borago officinalis L.: sources of gamma linolenic acid. Grasas Aceit., 44, 125–126.
del Río-Celestino, M., Font, R., de Haro-Bailón, A. (2008). Distribution of fatty acids in edible organs and seed fractions of borage (Borago officinalis L.). J. Sci. Food
Agric., 88, 248–255. DOI: 10.1002/jsfa
El Hafid, R., Blade, S.F., Hoyano, Y. (2002). Seeding date and nitrogen fertilization effects on the performance of borage (Borago officinalis L.). Ind. Crops Prod., 16(3),
193–199.
García-Iñiquez de Ciriano, M., García-Herreros, C., Larequi, E., Valencia, I., Ansorena, D., Astiasarán, I. (2009). Use of natural antioxidants from lyophilized water extracts of Borago officinalis in dry fermented sausages enriched in ω-3 PUFA. Meat Sci., 83, 271–277. DOI: 10.1016/j.meatsci.2009.05.009.
Gómez-Estaca, J., Montero, P., Gimenez, B., Gómez-Guillen, M.C. (2009). Incorporation of antioxidant borage extract into edible films based on sole skin gelatin
or commercial fish gelatin. J. Food Eng., 92, 78–85.
Gramza-Michałowska, A., Człapka-Matyasik, M. (2011). Evaluation of the antiradical potential of fruit and vegetable snacks. Acta Sci. Pol. Technol. Aliment., 10(1),
61–72. [Available: www.food.actapol.net].
Griffiths, G., Brechany, E.Y., Jackson, F.M., Christie, W.W., Stymne, S., Stobart, A.K. (1996). Distributionand biosynthesis of stearidonic acid in leaves of Borago
officinalis. Phytochemistry, 43(2), 381–386.
Gudej, J., Tomczyk, M. (1996). Badania chromatograficzne związków polifenolowych w zielu Borago officinalis (L.). Herba Pol., 42(4), 252–256.
Gunstone, F.D. (1992). Gamma-linolenic acid – occurrence and physical properties. Prog. Lipid Res., 31, 145–161.
Gupta, M., Singh S. (2010). Borago officinalis Linn. an important medicinal plant of Mediterranean region: a review. Int. J. Pharm. Sci. Review and Res., 5(1),
27–34. [Available: www. globalresearchonline.net].
Karłowicz-Bodalska, K., Bodalski, T. (2007). Nienasycone kwasy tłuszczowe, ich właściwości biologiczne i znaczenie w lecznictwie. Post. Fitoter., 1, 46–56.
Kołodziej, B., Berbeć, S. (2010). Czynniki zmienności związków aktywnych. In: Uprawa ziół. Poradnik dla plantatorów, Kołodziej B. (ed.). PWRiL, Poznań, 19–21.
Król, B. (2010). Ogórecznik lekarski. In: Uprawa ziół. Poradnik dla plantatorów, Kołodziej, B. (ed.). PWRiL, Poznań, 334–338.
Medrano, A., Masoud, T.A., Martinez, M.C. (1992). Mineral and proximate composition of borage. J. Food Comp. Anal., 5(4), 313–318.
Mhamdi, B., Wannes, W.A., Bourgou, S., Marzouk, B. (2009). Biochemical characterization of borage (Borago officinalis L.) seeds. J. Food Biochem., 33, 331–341.
Muuse, B.G., Essers, M.L., van Soest, L.J.M. (1988). Oenothera species and Borago officinalis: sources of gammalinolenic acid. Neth. J. Agric. Soc., 36, 357–363.
Osborne, J.L. (1999). Borage. Bee World, 80, 33–36. Polish Pharmacopoeia VI. Content of essential oil (2002). Polskie Towarzystwo Farmaceutyczne, Warszawa, 151.
Polish Pharmacopoeia IX (2011). Polskie Towarzystwo Farmaceutyczne, Warszawa.
Pieszak, M., Mikołajczak, P.Ł., Manikowska, K. (2012). Borage (Borago officinalis L.) – a valuable medicinal plant used in herbal medicine. Herba Pol., 58(4), 95–103.
PN-EN 14130:2004. Oznaczanie witaminy C metodą HPLC.
PN-EN ISO 734:2016-03. Śruta nasion oleistych. Oznaczanie zawartości oleju – Metoda ekstrakcji heksanem (lub benzyną lekką).
Peiretti, P.G., Palmegiano, G.B., Salamano, G. (2004). Quality and fatty acid content of borage (Borago officinalis L.) during the growth cycle. Ital. J. Food Sci., 16, 177–184.
Prior, R.L., Wu, X., Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric.
Food Chem., 53, 4290–4302.
Sánchez-Escalante, A., Djenane, D., Torrescano, G., Beltrán, J.A., Roncales, P. (2003). Antioxidant action of borage, rosemary, oregano, and ascorbic acid in beef patties packaged in modified atmosphere. J. Food Sci., 68(1), 339–344.
Sánchez-Moreno, C., Larrauri, J.A., Saura-Calixto, F. (1998). A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric., 76, 270–276.
Sayanova, O., Shevry, P.R., Napier, J.A. (1999). Characterization and expression of a fatty acid desaturase from Borago officinalis. J. Exp. Bot., 50, 411–412.
Segovia, F., Lupo, B., Peiró, S., Gordon, M.H., Almojano, M.P. (2014). Extraction of antioxidants from borage (Borago officinalis L.) leaves – optimization by response
surface method and application in oil-in-water emulsions. Antioxidants, 3, 339–357.
Shahidi, F. (2000). Antioxidant factors in plant foods and selected oilseeds. BioFactors, 13, 179–185.
Singleton, V.L., Rossi, J.A., jr (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16, 144–158.
Suchorska, K., Osińska, E. (1997). Some aspects of borage (Borago officinalis L.) cultivation. Part II. Influence of mother plant on the yield of borage seeds. Ann. Warsaw
Agric. Univ., Hortic., 18, 81–84.
Tasset-Cuevas, I., Fernández-Bedmar, Z., Lozano-Baena, M.D., Campos-Sánchez, J., de Haro-Bailón, A., Muñioz-Serrano, A., Alonso-Moraga, Á. (2013). Protective
Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies. PLoS One, 8(2), e56986.
Van Den Dool, H., Kratz, P.D. (1963). A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography.
J. Chromat., 11, 463–471.
Wettasinghe, M., Shahidi, F. (1999). Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago officinalis L.) seeds. Food
Chem., 67, 399–414.
Wettasinghe, M., Shahidi, F. (2000). Scavenging of reactive-oxygen species and DPPH free radicals by extracts of borage and evening primrose meals. Food Chem.,
70, 17–26.
Zadernowski, R., Polakowska-Nowak, H., Aleem, A.R., Kowalska, M. (1999). Lipidy nasion wiesiołka i ogórecznika. Rośl. Oleiste, 20(2), 581–589.
Zemmouri, H., Ammar, S., Boumendjel, A., Messarah, M., El Feki, A., Bouaziz M. (2014). Chemical composition and antioxidant activity of Borago officinalis L. leaf
extract growing in Algeria. Arab. J. Chem., XXX, 1–10. [Available: http://dx.doi.org/10.1016/j.arabjc.2014.11.059.
Zheng, W., Wang, S.Y. (2001). Antioxidant Activity and Phenolic Compounds in Selected Herbs. J. Agric. Food Chem., 49, 5165–5170.
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