Abstract
Endophytic fungi constitute an important yet still poorly explored component of the microbiome of thyme (Thymus spp.). This review summarizes the current knowledge on their diversity, ecological functions, and biotechnological potential based on literature published up to April 2026. Reported endophytes represent a diverse taxonomic community, comprising 49 genera and 53 species most of which belong to common ascomycete genera, such as Alternaria, Fusarium, Colletotrichum, Aspergillus, Penicillium, Phoma, and Trichoderma. Current evidence from experiments carried out in planta indicates that these microorganisms may contribute to plant growth promotion, enhanced tolerance to abiotic stress, modulation of essential oil production, and protection against plant pathogens through the synthesis of bioactive metabolites and volatile organic compounds. Moreover, in in vitro assays several isolates have demonstrated antimicrobial, antioxidant, cytotoxic, and enzyme-producing activities, highlighting their promising applications in sustainable agriculture and biotechnology. Although significant progress has been achieved, many aspects of the ecology, host specificity, and functional interactions of thyme-associated fungal endophytes remain insufficiently understood.
References
- Abdelhamid, S.A., Abo Elsoud, M.M., El-Baz, A.F. et al. (2024). Optimisation of indole acetic acid production by Neopestalotiopsis aotearoa endophyte isolated from Thymus vulgaris and its impact on seed germination of Ocimum basilicum. BMC Biotechnol. 24(1), 46. https://doi.org/10.1186/s12896-024-00872-3
- Abdel-Hamid, M.S., Fouda, A., El-Ela, H.K.A. et al. (2021). Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents. Biomol. Concepts 12(1), 175–196. https://doi.org/10.1515/bmc-2021-0019
- Adeleke, B.S., Fadiji, A.E., Ayilara, M.S. et al. (2022). Strategies to enhance the use of endophytes as bioinoculants in agriculture. Horticulturae 8(6), 498. https://doi.org/10.3390/horticulturae8060498
- Agrawal, S., Bhatt, A. (2023). Microbial endophytes: emerging trends and biotechnological applications. Curr. Microbiol. 80(8), 249. https://doi.org/10.1007/s00284-023-03349-2
- Ahmed, A.M., Mahmoud, B.K., Millán-Aguiñaga, N. et al. (2023). The endophytic Fusarium strains: a treasure trove of natural products. RSC Advan. 13(2), 1339–1369. https://doi.org/10.1039/d2ra04126j
- Ali, E.A.M., Aziz Ahmed, A.A., El-Ela Sayed, M.A. et al. (2018). Antioxidant activity and cytotoxicity of extracts of Thymus vulgaris L. and their associated endophytic fungi. Egypt. J. Experim. Biol. 14(1), 107–116. https://doi.org/10.5455/egy-jebb.20180122025355
- Bahadori, S., Attar, F., Osaloo, S.K. et al. (2026). Biogeographical and taxonomic insights into Iranian Thymus (Lamiaceae): Thymus coriifolius, and T. jankae and T. syriacus. Phytotaxa 744(2), 103–122. https://doi.org/10.11646/phytotaxa.744.2.1
- Bartolucci, F., Nachychko, V. (2025). The Euro+Med treatment of Thymus (Lamiaceae): nomenclature, typification of names and distribution updates. Willdenowia 55(1), 295–304. https://doi.org/10.3372/wi.55.14
- Bartolucci, F., Peruzzi, L., Passalacqua, N. (2013). Typification of names and taxonomic notes within the genus Thymus L. (Lamiaceae). Taxon 62(6), 1308–1314. https://doi.org/10.12705/626.1
- Celep, F., Dirmenci, T. (2017). Systematic and biogeographic overview of Lamiaceae in Turkey. Nat. Volat. Essent. Oils 4(4), 14–27. https://izlik.org/JA62MN24YT
- Checcucci, A., Maida, I., Bacci, G. et al. (2017). Is the plant-associated microbiota of Thymus spp. adapted to plant essential oil? Res. Microbiol. 168(3), 276–282. https://doi.org/10.1016/j.resmic.2016.11.004
- Crous, P.W., Sandoval-Denis, M., Costa, M.M. et al. (2022). Fusarium and allied fusarioid taxa (FUSA). 1. Fungal Syst. Evol. 9(1), 161–200. https://doi.org/10.3114/fuse.2022.09.08
- Delbari, Y., Mohassel, Y., Kakaei, E. et al. (2023). Identification and anti-bacterial property of endophytic actinobacteria from Thymes kotschyanus, Allium hooshidaryae, and Cerasus microcarpa. Sci. Rep. 13(1), 13145. https://doi.org/10.1038/s41598- 023-40478-x
- Del Valle, J.C., Martín-Carretié, E.M., García-Cárdenas, F.J. et al. (2026). Ecological and genetic determinants of essential oil diversity in Mediterranean Thymus. Biochem. Syst. Ecol. 127, 105260. https://doi.org/10.1016/j.bse.2026.105260
- Dolatabadi, H.K., Goltapeh, E.M., Moieni, A. et al. (2011). Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments. Symbiosis 53(1), 29–35. https://doi.org/10.1007/ s13199-010-0104-0
- Drew, B.T., Sytsma, K.J. (2012). Phylogenetics, biogeography, and staminal evolution in the tribe Mentheae (Lamiaceae). Am. J. Bot. 99(5), 933–953. https://doi.org/10.3732/ajb.1100549
- El-Ela Sayed, M.A., Ali E.A.M., Aziz Ahmed, A.A. et al. (2020). Antimicrobial activity of Thymus vulgaris L. and associated endophytes extracts. Egypt. J. Experim. Biol. 16(2), 141. https://doi.org/10.5455/egyjebb.20200709014142
- Etri, K., Pluhár, Z. (2024). Exploring chemical variability in the essential oils of the Thymus genus. Plants 13(10), 1375. https://doi. org/10.3390/plants13101375
- Federici, S., Galimberti, A., Bartolucci, F. et al. (2013). DNA barcoding to analyse taxonomically complex groups in plants: the case of Thymus (Lamiaceae). Bot. J. Linnean Soc. 171(4), 687–699. https://doi.org/10.1111/boj.12034
- Fisher, P.J., Petrini, O., Amezquita, M.M. (1992). Endophytic fungi from Alpine and Mediterranean species of Thymus. Nova Hed¬wigia 55(3–4), 473–477.
- García‐Cárdenas, F.J., Ortiz, M.Á., del Valle, J.C. et al. (2026). Patterns of interploidy admixture in polyploid complexes: insights from Thymus sect. Mastichina (Lamiaceae). Mol. Ecol. 35(3), e70259. https://doi.org/10.1111/mec.70259
- Gupta, A., Meshram, V., Gupta, M. et al. (2023). Fungal endophytes: Microfactories of novel bioactive compounds with therapeutic interventions. A comprehensive review on the biotechnological developments in the field of fungal endophytic biology over the last decade. Biomolecules 13(7), 1038. https://doi.org/10.3390/biom13071038
- Halat, D.H., Krayem, M., Khaled, S. et al. (2022). A focused insight into thyme: Biological, chemical, and therapeutic properties of an indigenous Mediterranean herb. Nutrients 14(10), 2104. https://doi.org/10.3390/nu14102104
- Kamyab, A., Samsampour, D., Ahmadinasab, N., Bagheri, A. (2024). Lamiaceae family-derived endophytic fungi: induced tolerance to drought stress in Thymus vulgaris plants. BMC Plant Biol. 24(1), 1104. https://doi.org/10.1186/s12870-024-05764-4
- Li, J.F., Jiang, H.B., Jeewon, R. et al. (2022). Alternaria: Update on species limits, evolution, multi-locus phylogeny, and classification. Stud. Fungi 8(1), 1–61. http://doi.org/sif-0024-0001
- López, P., Pastrana, A.M., Marin, M.V. et al. (2026). Molecular characterization reveals three Neopestalotiopsis species causing strawberry disease outbreaks in Spain. Front. Plant Sci. 17, 1830265. https://doi.org/10.3389/fpls.2026.1830265
- Ma, L., Qu, H., Guo, Z. et al. (2023). Identification and antifungal activity of endophytic fungus Trichoderma samuelsii 2-63 strain from Thymus mongolicus Ronn. Chin. J. Pest. Sci. 25(2), 377–387. https://doi.org/10.16801/j.issn.1008-7303.2023.0012
- Machowicz-Stefaniak, Z., Zimowska, B., Zalewska, E. (2002). Fungi colonizing various organs of thyme (Thymus vulgaris L.) cultivated in the region of Lublin. Acta Agrobot. 55(1), 185–197.
- Machowicz-Stefaniak, Z., Zimowska, B., Zalewska, E. (2008). The occurrence and pathogenicity of Phoma exigua Desm. var. exigua for selected species of herbs. Acta Agrobot. 61(2), 157–166. https://doi.org/10.5586/aa.2008.044
- Malagaris, P. (2011). Biology and ecology of Asphondylia coridothymi (Diptera: Cecidomyiidae) inducing galls on Coridothymus capitatus on the island of Samos, Greece. Acta Soc. Zool. Bohem. 75, 239–251.
- Manathunga, K.K., Gunasekara, N.W., Meegahakumbura, M.K. et al. (2024). Exploring endophytic fungi as natural antagonists against fungal pathogens of food crops. J. Fungi 10(9), 606. https://doi.org/10.3390/jof10090606
- Masumi, S., Mirzaei, S., Kalvandi, R. et al. (2014). Asparaginase and amylase activity of thyme endophytic fungi. J. Crop Prot. 3(5), 655–662. https://doi.org/10.48311/jcp.2014.1176
- Masumi, S., Mirzaei, S., Zafari, D. et al. (2015a). Isolation, identification and endophytic bacteria and yeasts isolated from Thymus. Progr. Biol. Sci. 5(1), 33–42.
- Mirzaei, S., Masumi, S. (2023). The antimicrobial activity of endophytic fungi isolated from Thymus spp. J. Med. Plants By-prod. 1, 117–124. https://doi.org/10.22092/jmpb.2022.342405.1192
- Mohamad, O.A.A., Ma, J.B., Liu, Y.H. et al. (2020). Beneficial endophytic bacterial populations associated with medicinal plant Thymus vulgaris alleviate salt stress and confer resistance to Fusarium oxysporum. Front. Plant Sci. 11, 47. https://doi.org/10.3389/fpls.2020.00047
- Mohamad, O.A.A., Liu, Y.H., Huang, Y. et al. (2022). The metabolic potential of endophytic actinobacteria associated with medicinal plant Thymus roseus as a plant-growth stimulator. Microorganisms 10(9), 1802. https://doi.org/10.3390/microorganisms10091802
- Morales, R. (2002). The history, botany and taxonomy of the genus Thymus. In: Thyme: The Genus Thymus, Stahl-Biskup, E., Sáez, F. (eds.). Taylor & Francis, London, 1–44.
- Morelli, M., Bahar, O., Papadopoulou, K.K. et al. (2020). Role of endophytes in plant health and defense against pathogens. Front. Plant Sci. 11, 1312. https://doi.org/10.3389/fpls.2020.01312
- Musa, Z., Ma, J., Egamberdieva, D. et al. (2020). Diversity and antimicrobial potential of cultivable endophytic actinobacteria asso¬ciated with the medicinal plant Thymus roseus. Front. Microbiol. 11, 191. https://doi.org/10.3389/fmicb.2020.00191
- Najar, B., Pistelli, L., Ferri, B. et al. (2021). Crop yield and essential oil composition of two Thymus vulgaris chemotypes along three years of organic cultivation in a hilly area of central Italy. Molecules 26(16), 5109. https://doi.org/10.3390/molecules26165109
- Nicoletti, R., Di Vaio, C., Cirillo, C. (2020). Endophytic fungi of olive tree. Microorganisms 8(9), 1321. https://doi.org/10.3390/microorganisms8091321
- Nicoletti, R., Fiorentino, A. (2015). Plant bioactive metabolites and drugs produced by endophytic fungi of Spermatophyta. Agriculture 5(4), 918–970. https://doi.org/10.3390/agriculture5040918
- Nicoletti, R., Zimowska, B. (2023). Endophytic fungi of hazelnut (Corylus avellana). Plant Prot. Sci. 59(2), 107–123. https://doi.org/10.17221/133/2022-PPS
- Nieto, G. (2020). A review on applications and uses of Thymus in the food industry. Plants 9(8), 961. https://doi.org/10.3390/plants9080961
- Nieto-Lugilde, D., Del Valle, J.C., Martín-Carretié, E.M. et al. (2026). Genomic structure, introgression, niche overlap, and morphological variation challenges species delimitation in Thymus sect. Mastichina. Ann. Bot. 138(4), 1194–1209. https://doi.org/10.1093/aob/mcag151
- Olama, S.M., Sheata, R., El Shiekh, H. et al. (2023). Phytochemistry and biodiversity of endophytic fungal metabolites isolated from medicinal plants. Al-Azhar J. Agric. Res. 48(2), 42–58. https://doi.org/10.21608/ajar.2024.224023.1198
- Pandey, S.S., Jain, R., Bhardwaj, P. et al. (2022). Plant probiotics–endophytes pivotal to plant health. Microbiol. Res. 263, 127148. https://doi.org/10.1016/j.micres.2022.127148
- Rabiey, M., Hailey, L.E., Roy, S.R. et al. (2019). Endophytes vs tree pathogens and pests: can they be used as biological control agents to improve tree health? Eur. J. Plant Pathol. 155(3), 711–729. https://doi.org/10.1007/s10658-019-01814-y
- Ray, P., Craven, K.D. (2016). Sebacina vermifera: a unique root symbiont with vast agronomic potential. World J. Microbiol. Bio¬technol. 32(1), 16. https://doi.org/10.1007/s11274-015-1970-7
- Saleem, S., Sekara, A., Pokluda, R. (2022). Serendipita indica – A review from agricultural point of view. Plants 11(24), 3417. https://doi.org/10.3390/plants11243417
- Soorni, A., Borna, T., Alemardan, A. et al. (2019). Transcriptome landscape variation in the genus Thymus. Genes 10(8), 620. https://doi.org/10.3390/genes10080620
- Waheed, M., Hussain, M.B., Saeed, F. et al. (2024). Phytochemical profiling and therapeutic potential of thyme (Thymus spp.): A medicinal herb. Food Sci. Nutri. 12(12), 9893–9912. https://doi.org/10.1002/fsn3.4563
- Yadav, A.N., Kour, D., Kaur, T. et al. (2022). Endophytic fungal communities and their biotechnological implications for agro-envi¬ronmental sustainability. Folia Microbiol. 67(2), 203–232. https://doi.org/10.1007/s12223-021-00939-0
- Zerroug, A., Sadrati, N. (2022). Endophytic fungi isolated from Thymus algeriensis with good antimicrobial activities. Turk. J. Agric. Food Sci. Technol. 10(10), 1789–1798. https://doi.org/10.24925/turjaf.v10i10.1789-1798.5395
- Zhao, M., Su, H., Huang, Y. et al. (2023). Plant growth-promoting properties and anti-fungal activity of endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta in arid lands. J. Arid Land 15(11), 1405–1420. https://doi.org/10.1007/s40333-023-0071-y
- Zimowska, B. (2022). Phoma on medicinal and aromatic plants. In: Phoma: Diversity, taxonomy, bioactivities, and nanotechnology. Springer International Publishing, Cham, 37–55.
- Zimowska, B., Becchimanzi, A., Krol, E.D. et al. (2021). New Cladosporium species from normal and galled flowers of Lamiaceae. Pathogens 10(3), 369. https://doi.org/10.3390/pathogens10030369
- Zimowska, B., Okoń, S., Becchimanzi, A. et al. (2020). Phylogenetic characterization of Botryosphaeria strains associated with Asphondylia galls on species of Lamiaceae. Diversity 12(2), 41. https://doi.org/10.3390/d12020041
- Zimowska, B., Viggiani, G., Nicoletti, R. et al. (2017). First report of the gall midge Asphondylia serpylli on thyme (Thymus vulgaris), and identification of the associated fungal symbiont. Ann. Appl. Biol. 171(1), 89–94. https://doi.org/10.1111/aab.12360
- Zubek, S., Błaszkowski, J. (2009). Medicinal plants as hosts of arbuscular mycorrhizal fungi and dark septate endophytes. Phyto¬chem. Rev. 8(3), 571–580. https://doi.org/10.1007/s11101-009-9135-7
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