ANTIFUNGAL ACTIVITY OF SOME PLANT EXTRACTS AGAINST Botrytis cinerea Pers. IN THE BLACKCURRANT CROP (Ribes nigrum L.)
Tatiana Eugenia Sesan
University of Bucharest, RomaniaElena Enache
University of Bucharest, RomaniaBeatrice Michaela Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, RomaniaMaria Oprea
Research-Development Institute of Plant Protection Bucharest, RomaniaFlorin Oancea
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, RomaniaCristian Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, RomaniaAbstract
There were tested and screened, in vitro and in vivo, for the first time in Romania, nine respectively six plant extracts manufactured by Hofigal S.A. against Botrytis
cinerea (strain Bc 27) isolated from blackcurrant (Ribes nigrum L.). The highest antibotrytis in vitro activity (efficiency between 80 and 100%) was obtained using the following extracts: Hyssopus officinalis (at 20, 10 and 5%), Satureja hortensis, Allium sativum, Tagetes patula (at 20 and 10%) and Mentha (at 20%). A moderate antibotrytis activity (efficiency between 35.7 and 65.7%) has been noticed for Mentha (at 10 and 5%), Satureja hortensis, Allium sativum and Tagetes patula (at 5%) extracts. The lowest antibotrytis activity or no efficiency was noticed using extracts obtained from Achillea millefolium, Artemisia dracunculus ‘sativa’, Rosmarinus officinalis and Valeriana officinalis even applied at 20%. Based on results obtained in in vitro tests, six plant extracts were tested and screened in vivo, under field conditions at Hofigal S.A. Bucharest. Satureja hortensis, Allium sativum, Hyssopus officinalis, Menthaand Tagetes patula extracts have been efficient in limiting gray mold severity in blackcurrant applied at 10% compared to untreated control. No in vivo activity was registered for Valeriana officinalis extract. Plant extracts with highly efficiency can be recommended as a non-polluting and environmental-friendly alternative (organic horticulture) in the protection of blackcurrant as medicinal crop against grey mould, the most economically important disease in Europe at present.
Keywords:
blackcurrant, Botrytis cinerea, plant extracts, organic horticulture, RomaniaReferences
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Daferera, D.J., Ziogas, B.N., Polissiou, M.G. (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusariumand Clavibacter michiganense subsp. michiganensis. Crop Prot., 22(1), 39–44.
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Dubey, N.K. (2011). Natural products in plant pest management. UK, 293 pp.
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El Oirdi, M., Bouarab, K. (2007). Plant signalling components EDS1 and SGT1 enhance disease caused by the necrotrophic pathogen Botrytis cinerea. New Phytol., 175, 131–139.
Gonzales-Collado, I., Macias-Sachez, A.J., Hanson, J.R. (2006). Fungal terpene metabolites: biosynthetic relationships and the control of phytopathogenic fungus Botrytis cinerea. Nat. Prod. Rep., 24, 674–688.
Han, Y., Xiao, D., Xiang, Y., Ye, L., Cheng, C. (2000). Study on the volatile oil of Nardostachys chinensis. Zhong Yao Cai, 23, 34–35.
Hébért, C., Charles, M.T., Gauthier, L., Willemot, C., Khanizadeh, S., Cousineau, J. (2002). Strawberry proanthocyanidins: biochemical markers for Botrytis cinerea resistance and selflife predicitability. Acta Horticult., 567, 659–662.
Huang, Jenn-Wen, Chung, Wen-Chuan (2003). Management of vegetable crop diseases with plant extracts. In: Advances in plant management, Huang, H.C., Surya A. (eds), 153–163.
Iacomi, B., Gherghieş, C., Enciu, S., Manole, M. (2000). Phyto-extracts with antifungal activity, Lucrări Ştiinţifice. USAMV Buc., ser. B, vol. 18, 137–139.
Ivănescu, B. (2010). Studiul fitochimic al unor compuşi din speciile Artemisia absinthium, A. vulgaris şi A. annua recoltate din flora spontană. Rezumat teza de doctorat. Univ. de Medicină şi Farmacie „Gr. T. Popa”, Facultatea de Farmacie, Iaşi.
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Tatiana Eugenia Sesan
University of Bucharest, Romania
University of Bucharest, Romania
Elena Enache
University of Bucharest, Romania
University of Bucharest, Romania
Beatrice Michaela Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
Maria Oprea
Research-Development Institute of Plant Protection Bucharest, Romania
Research-Development Institute of Plant Protection Bucharest, Romania
Florin Oancea
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
National Research-Development Institute for Chemistry and Petrochemistry Bucharest, Romania
Cristian Iacomi
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
University of Agricultural Sciences and Veterinary Medicine Bucharest, Romania
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