COMPARATIVE STUDIES ON THE AGRONOMIC VALUE OF in vitro AND CONVENTIONALLY PROPAGATED STRAWBERRY (Fragaria × ananassa Duch.) PLANTS
Jadwiga ŻebrowskaUniversity of Life Sciences in Lublin
Elżbieta PogroszewskaUniversity of Life Sciences in Lublin
Jacek GawrońskiUniversity of Life Sciences in Lublin
In principle, in vitro propagation results in uniform batches of plants, which grow, flower and fruit normally. However, phenotypic changes often observed in the field performance of tissue culture-produced strawberry plants might affect their agronomic value. So, it is of utmost importance to assess the field performance of in vitro propagated plants to verify their fidelity to conventional propagated plants. In this study, the agronomic value of the strawberry plants derived from cultivars ‘Filon’ and ‘Teresa’ via in vitro propagation and their first vegetative progeny was compared to conventional plants. During the field experiments, agronomic traits such as plant vigor, abundance of flowering and yield components were evaluated. The results showed the different field response of cultivars tested to in vitro propagation. In spite of the phenotypic changes observed in in vitro derived plants, their agronomic value was equal or superior in comparison with conventional plants. In conclusion, it should be stated that in vitro propagation method can be safely recommended for the reproduction of these strawberry cultivars.
Keywords:micropropagation, microplants, phenotypic uniformity, runner seedlings, tissue culture
Biswas, M.K., Dutt, M., Roy, U.K., Islam, R., Hossain, M. (2009). Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Sci. Hortic., 122, 409–416.
Borkowska, B. (2001). Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro. Sci. Hortic., 89, 195–206.
Boxus, Ph. (1992). Mass production of strawberry and new alternatives for some horticultural crops. In: Proceedings of the International Symposium on Transplant Production Systems, 21–26 July, Kurata, K., Kozai, T. (eds). Yokohama, Japan, 151–162.
Boxus, Ph., Jemmali, A., Terzi, J.M., Arezki, O. (2000). Drift in genetic stability in micrporopagation: the case of strawberry. Acta Hort., 530, 155–162.
Boxus, Ph., Quoirin, M., Laine, M.J. (1977). Large scale propagation of strawberry plants from tissue culture. In:. Applied and fundamental aspects of plant cell, tissue and organ culture, Reinert, J., Bajaj, Y.P.S. (eds.). New York, Springer-Verlag, 130–143.
Debnath, S.C. (2005). Strawberry sepal: another explant for thidiazuron-induced adventitious shoot regeneration. In Vitro Cell. Dev. Pl., 41, 671–676.
Debnath, S.C. (2006). Zeatin overcomes thidiazuron-induced inhibition of shoot elongation and promotes rooting in strawberry culture in vitro. J. Hortic. Sci. Biotech., 81, 349–354.
Gantait, S., Mandal, N., Das, P.K. (2010). Field performance and molecular evaluation of micropropagated strawberry. Rec. Res. Sci. Tech., 2, 12–16.
Graham, J. (2005). Fragaria Strawberry. In: Biotechnology of fruit and nut crops, Litz, R. (ed.). Biotechnology in Agriculture Series No 29, CAB International, Wallingford, UK, 456–474.
Hammerschlag, F., Garces, S., Koch-Dean, M., Ray, S., Lewers, K., Maas, J., Smith, B. (2006). In vitro response of strawberry cultivars and regenerants to Colletotrichum acutatum. Plant Cell Tiss. Org. Cult., 84, 255–261.
Kaeppler, S.M., Kaeppler, H.F., Rhee, Y. (2000). Epigenetic aspect of somaclonal variation in plants. Plant Mol. Biol., 43, 179–188.
Karhu, S., Hakala, K. (2002). Micropropagated strawberries on the field. Acta Hort., 567, 321–323.
Litwińczuk, W. (2004). Field performance of Senga Sengana strawberry plants (Fragaria × ananassa Duch.) obtained by runners and in vitro through axillary and adventitious shoots. Horticulture, EJPAU, 7, 03.
Lucyszyn, N., Quoirin, M., Koehler, H.S., Reicher, F., Sierakowski, M.R. (2006). Agar/galactomannan blends for strawberry (Fragaria × ananassa Duchesne) cv. Pelican micropropagation. Sci. Hortic., 107, 358–364.
Mahajan, R., Kaur, R., Shama, A., Shama, D.R. (2001). Micropropagation of strawberry cultivar Chandler and Fern. Crop Improv., 28, 19–25.
Miguel, C., Marum, L. (2011). An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. J. Exp. Bot., 62, 3713–3725.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant., 15, 473–497.
Official Journal of EU (2007). OJ L 299, 16.11.2007, vol. 50, 1–149.
Passey, A.J., Barrett, K.J., James, D.J. (2003). Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types. Plant Cell Rep., 21, 397–401.
Sowik, I., Bielenin, A., Michalczuk, L. (2001). In vitro testing of strawberry resistance to Verticillium dahliae and Phytophthora cactorum. Sci. Hort., 88, 31–40.
Szczygieł, A., Pierzga, K., Borkowska, B. (2002). Performance of micropropagated strawberry plantlets after planting in the field. Acta Hort., 567, 317–320.
Żurawicz, E., Bielenin, A., Lisek, J., Łabanowska, B.H., Mochecki, J., Treder, W. (2005). Metodyka integrowanej produkcji truskawek. Plantpress. Kraków.
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.