THE INFLUENCE OF THE SALT COMPOSITION OF BASAL MEDIUM AND GROWTH REGULATORS ON IN VITRO GROWTH AND DEVELOPMENT OF Tibouchina urvilleana (DC.) Cogn.
Danuta Kozak
University of Life Sciences in LublinKatarzyna Wnuk
University of Life Sciences in LublinAbstract
Tibouchina urvilleana is a very popular small tree or shrub known for its beautiful velvety foliage and violet-blue flowers. It can be used in the mild climate in any
landscape or as an indoor container plant in cooler regions. The aim of the present study was to determine the effect of mineral salt composition and growth regulators (BA and IAA) on the growth and development of Tibouchina urvilleana shoots. Shoot tips, obtained from aseptically grown shoots, were cultured on Murashige and Skoog (MS) (full and half strength), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA at 2.5 mg·dm-3 and IAA at 0.1 mg·dm-3. Also, the effects of BA (1.0, 2.5, 5.0 mg·dm-3) and IAA (0.1 or 0.5 mg·dm-3) applied alone or in combination were investigated. Explants cultured on medium without growth substances were used as a control. The study results showed that the application of full-strength Murashige and Skoog salt mixture has a more beneficial effect on the growth of the main shoot and the induction of axillary shoots of Tibouchina urvilleana compared to ½ MS, WPM and NN salt mixtures. The number of axillary shoots increases with the increasing concentration of BA (from 1 to 5 mg·dm-3), but they are characterized by a smaller length. The combined
application of BA and IAA does not have a significant effect on the induction of axillary shoots. The addition of IAA at a concentration of 0.5 mg·dm-3 to the media containing BA (1 to 5 mg·dm-3) has a beneficial effect on elongation growth of axillary shoots.
Keywords:
Tibouchina urvilleana, mineral salt mixture, cytokinin, auxin, branching, micropropagationReferences
Al-Juboory K.H., 1996. Shoot proliferation of Gardenia jasminoides in vitro. HortSci. 31(4), 587–588.
Bashir M.A., Rashid H., Anjum M.A., 2007. In vitro shoot multiplication of six promising strains of jojoba (Simmondsia chinensis). Biotech. 6 (3), 309–315.
Desphande S.R., Josekutty P.C., Prathepasenan G., 1998. Plant regeneration from axillary buds of a mature tree of Ficus religiosa. Plant Cell Rep. 17, 571–573.
Ebrahim M.K.H., Ibrahim I.A., 2000. In vitro growth and development of Maranta leuconeura (cv. Kerchoviana) as affected by medium gelation and pH value. Egyptian J. Hort. 27(1), 55–67.
Furmanowa M., Oledzka H., Józefowicz J., Pietrosiuk A., 1994. Catharanthus roseus (L) F.G. Don-plant regeneration and alkaloid content. Acta Soc. Bot. Poloniae 63 (2), 179–184.
Gamborg O.L., Miller R.A., Ojima K., 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158.
He Ch., Dai S., Ma G., 2009. Callus induction and plant regeneration in Tibouchina urvilleana (DC.) Cogn. Propagation of Ornam. Plants. 9 (2), 59–64.
Jiang H.-R., Yu F.-X., Liu T.-Y., 2005.Tissue culture and high frequency propagation of Callistemon rigidus. R. Br. Acta Hort. Sinica 32 (3), 541–543.
Karim M.Z., Amin M.N., Azad M.A.K., Begum F., Rahman M.M., Islam M.M., Alam R., 2003. Effect of different plant growth regulator on in vitro shoot multiplication of Chrysanthemum morifolium. OnLine J. Biol. Sci. 3 (6) 553–560.
Kozak D., Wnuk K., 2005. The influence of cytokinins on the formation and growth of Tibouchina urvilleana Cogn. axillary shoots in vitro. Zesz. Probl. Post. Nauk Roln. 504, 127–133.
Kozak D., Hetman J., Witek M., 2007. The influence of mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots. Acta Agrobot. 60 (1), 95–99.
Kukreja A.K., 1999. In vitro propagation of lemon balm (Melissa officinalis L.) through nodal bud culture of adult plant. J. Spec. Aroma. Crops 8 (2), 171–177.
Kyte L., 1990. Plants from test tubes. Timber Press, Portland, Oregon.
Lloyd G., McCown B.H., 1980. Commercially – feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Comb. Proc. 30, 421–427.
Lu M.-Ch., 2002. Micropropagation of Morus latifolia Poilet using axillary buds from mature trees. Sci. Hort. 96, 329–341.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.
Nitsch J.P., Nitsch C., 1969. Haploid plants from pollen grains. Science, 163, 85–87.
Onagro V., Leyser O., 2008. Hormonal control of shoot branching. J. Exp. Bot. 59, 67–74.
Poospooragi R., 2005. Micropropagation and effect of growth retardants on selected species of Melastomataceae. PhD thesis, Universiti Putra Malaysia.
Ramage C.M., Williams R.R., 2002. Mineral nutrition and plant morphogenesis. In Vitro Cell Dev. Biol. – Plant 38, 116–124.
Shabbir A., Hameed M., Ali A., Bajwa R., 2009. Effect of different cultural conditions of micropropagation of rose (Rosa indica L.). Pak. J. Bot. 41 (6), 2877–2882.
Shahzad A., Parveen S., Fatema M., 2011. Development of a regeneration system via nodal segment culture in Veronica anagallis-aquatica L. – an amphibious medicinal plant. J. Plant Interactions 6 (1), 61–68.
Shekafandeh A., 2007. Effect of different growth regulators and source of carbohydrates on in and ex vitro rooting of iranian myrtle. Inter. J. Agric. Res. 2 (2), 152–158.
Sumana K.R., Kaveriappa K.M., 2000. Micropropagation of Lagerstroemia reginae Roxb. through shoot bud culture. Indian J. Plant Physiol. 5 (3), 232–235.
Sun Y., Zhang D., Smagula J., 2010. Micropropagation of Ilex glabra (L.) A. Gray. HortSci. 45 (5), 805–808.
Zobayed S.M.A., 2000. In vitro propagation of Lagerstroemia spp. from nodal explants and gaseous composition in the culture headspace. Environ. Control in Biol. 38 (1), 1–11.
Yalcin-Mendi Y., Unek C., Eldogan S., Akakacar Y., Serce S., Curuk P., Kocaman E., 2009. The effect of different hormones on regeneration of gazania (Gazania rigens). Rom. Biotechnol. Lett. 14 (5), 4728–4732.
University of Life Sciences in Lublin
University of Life Sciences in Lublin
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