Ewa Mirzwa-Mróz

Warsaw University of Life Sciences – SGGW

Marzena Wińska-Krysiak

Warsaw University of Life Sciences – SGGW


Sooty blotch is one of the most common disease of apples in organic orchards in many countries. Results of molecular studies performed in USA indicated approximately 30 different fungi species associated with this disease. Fungi species causing sooty blotch in Northern, Central and Eastern Poland were identified on the basis of morphology and nucleotide sequence of the rDNA internal transcribed spacer region (ITS). A total 245 isolates were collected in spring and early summer in the years 2006–2009 from fruits with visible symptoms of the disease. Isolates were grown on PDA medium and identified on the basis of morphological characters. DNA was extracted from representative isolates and used as matrices for PCR amplification with ITS1F and ITS4 primers. Fragments of amplified rDNA ITS were sequenced. It was found that 66.53% of all isolates causing sooty blotch were species from genera Microcyclosporella, followed by Aureobasidium
pullulans – 22.86%, Microcyclospora sp. – 6.12%, Phialophora sessilis – 3.67%, Peltaster sp. and P. fructicola – 0.41%.


apple, Microcyclosporella sp., Microcyclospora sp., Peltaster fructicola

Abad G., Raleigh N.C., Sutton T.B., 2005. Morphological and molecular identification of Stomiopeltis uniloculata sp. nov. and Stomiopeltis multiloculata sp. nov. associated with apple fruit in North Carolina. Phytopathology 95(6), 960.
Baines R.C., Gardner M.W., 1932. Pathogenicity and cultural characters of the apple sooty-blotch fungus. Phytopathology 22(12), 937–952.
Batzer J.C., Gleason M.L., Harrington T.C., Tiffany L.H., 2005. Expansion of the sooty blotch and flyspeck complex on apples based on analysis of ribosomal DNA gene sequences and morphology. Mycologia 97(6), 1268–1286.
Braun U., 1995. A monograph of Cercosporella, Ramularia and allied genera (phytopathogenic Hyphomycetes). Vol. 1. IHW-Verl.
Braun U., 2000. Miscellaneous notes on some micromycetes. Schlechtendalia 5, 31–56.
Brown E.M., Sutton T.B., 1993. Time of infection of Gloeodes pomigena and Shizothyrium pomi on apple in North Carolina and potential control by an eradicant spray program. Plant Dis. 77, 451–455.
Deighton F.C., 1973. Studies on Cercospora and allied genera. IV. Cercosporella Sacc., Pseudocercosporella gen. nov. and Pseudocercosporidium gen. nov. Mycol. Pap. 133, 1–62.
Díaz Arias M.M., Batzer J.C., Harrington T.C., Wong A.W., Bost S.C., Cooley D.R., Ellis M.A., Hartman J.R., Rosenberger D.A., Sundin G.W., Sutton T.B., Travis J.W., Wheeler M.J., Yoder K.S., Gleason M.L., 2010. Diversity and biogeography of sooty blotch and flyspeck fungi on apple in the Eastern and Midwestern United States. Phytopathology 100, 345–355.
Frank J., Crous P.W., Groenewald J.Z., Oertel B., Hyde K.D., Phengsintham P., Schroers H.-J., 2010. Microcyclospora and Miclocyclosporella: novel genera accomodating epiphytic fungi causing sooty blotch on apple. Persoonia 24, 93–105.
Gardes M., Bruns T.D., 1993. ITS primers with enhanced specificity for basidiomycetesapplication to the identification of mycorrhizae and rusts. Molecular Ecology 2, 113–118.
Grabowski M., 2004. Etiology of sooty blotch in selected regions of southern Poland. Phytopath. Pol. 35, 73–81.
Grabowski M., 2007. The study of new fungus species causing apple sooty blotch. Folia Horticulturae, Ann. 19(2), 89–97.
Groves A.B., 1933. A study of the sooty blotch disease of apples and the casual fungus Gloeodes pomigena. Virginia Agric. Exper. Stat. Tech. Bull. 50, 1–43.
Ivanović M.M., Ivanović M.S., Batzer J.C., Tatalović N., Oertel B., Latinović J., Latinović N., Gleason M.L., 2010. Fungi in the apple sooty blotch and flyspeck complex from Serbia and Montenegro. J. Plant Pathol. 92(1), 65–72.
Johnson E.M., Sutton T.B., Hodges C.S., 1996. Peltaster fructicola: a new species in the complex of fungi causing apple sooty blotch disease. Mycologia 88(1), 114–120.
Johnson E.M., Sutton T.B., Hodges C.S., 1997. Etiology of apple sooty blotch disease in North Carolina. Phytopathology 87, 88–95.
Kirk P.M., Cannon P.F., Minter D.W., Stalpers J.A., 2008. Ainsworth and Bisby’s dictionary of the fungi. 10 ed. CABI Bioscience, CAB International. Wallingford.
Lohrer T., 2004. Regenfleckenkrankheit und Fliegenschmutzfleckenkrankheit. Infoblatter, http://www.fh-weihenstephan.de/fgw/wissenspool/infos/kurzinfo.ph (verified April 13, 2005).
Marcinkowska J., 2004. Oznaczanie rodzajów grzybów ważnych w patologii roślin. Fundacja Rozwój SGGW. Warszawa.
Nelson S., 2008. Sooty mold. Plant Disease PD-52, http://www.ctahr.hawaii.edu/oc/freepubs/pdf/PD-52.pdf (verified May 10, 2011)
Noga G., Kern S., Dehne W., Steiner U.Bohmer B., Heupel M., Engel A., Frahm J., 2000. Die Ruβflecken krankheit beim Apfel. http://www.usl.uni-bonn.de/pdf/russfleck.pdf (verified June09, 2011).
Tarnowski T., Batzer J., Gleason M., Helland S., 2003. Sensitivity of newly identified clades in the sooty blotch and flyspeck complex on apple to thiophanate-methyl and ziram. Plant Management Network, pp. 15–21.
White T.J., Bruns T., Taylor J, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. [In:] Innis MA, Gelfand D.H., Snisky J.J., White J.W. eds. PCR protocols: a guide to molecular methods and amplifications. New York. Academic Press., 315–322.
Wilcox W.F., 1994. Sooty blotch Gloeodes pomigena (Schwein.) Colby and flyspeck Shizothyrium pomi (Mont. and Fr.) Arx. Tree Fruit Crops, Disease Identification Sheet, D11, pp. 4.
Williamson S.M., Hodges C.S., Sutton T.B., 2004. Re-examination of, a member of the apple sooty blotch complex. Mycologia 96(4), 885–890.
Williamson S.M., Sutton T.B., 2000. Sooty blotch and flyspeck of apple: etiology, biology, and control. Plant Disease 84(7), 714–724.
Wrona B., Grabowski M., 2004. Etiology of the apple sooty blotch in Poland. J. Plant Protection Res. 44(4), 294–297.


Ewa Mirzwa-Mróz 
Warsaw University of Life Sciences – SGGW
Marzena Wińska-Krysiak 
Warsaw University of Life Sciences – SGGW



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