Determination of the quality and phytochemical content of F1 strawberry genotypes selected in Bilecik province ecological conditions

Sinem Ozturk Erdem

Department of Horticulture, Faculty of Agriculture and Natural Science, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey

Cetin Cekic

Department of Horticulture, Faculty of Agriculture, Tokat Gaziosmanpasa University, 60240, Tasliciftlik, Tokat, Turkey

Davut Uysal

Department of Horticulture, Faculty of Agriculture and Natural Science, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey


The production of strawberry cultivation in wide ecological regions, its aroma, rich mineral content and taste affect the preference of consumers positively. The high amount of aroma of local cultivars enables them to be used as parents in breeding studies. In this study, it was aimed to determine the morphological and pomological characteristics and phytochemical contents of the superior F1 strawberry genotypes selected after hybridization with the local Osmanli strawberry under the ecological conditions of Bilecik province. Morphological and pomological analyzes were made and the best ten genotypes were selected according to the weighted grading method. According to this, the titratable acidity amount of the selected genotypes was determined between 0.28–0.51, the total soluble solid amount was determined between 5–10.5%, and the pH amount was determined between 3.3–3.78. By looking at the average fruit weight, fruit width and fruit length values, genotypes that can compete with commercial cultivars were selected and these were determined as table genotypes. While the total phenolic content of the ten genotypes selected by phytochemical analyzes was found between 2675.56–3983.89 μg GAE/g TA, the total amount of anthocyanin was determined between 156.19–39.12 μg Plg-3-glu/gta. The antioxidant amount was 18.95 μmol TE/ g ta-7.54 μmol TE/ g in the FRAP method.


anthocyanin, antioxidant, breeding, Fragaria ananassa, Osmanli strawberry

Akbas, F.C., Sarıdaş, M.A., Ağçam, E., Keskinaslan, G.S., Kamar, R., Paydaş Kargı, S. (2021). Evaluation of important strawberry genotypes in terms of taste parameters. Turk. J. Agric. – Food Sci. Technol., 9(1), 241–248. DOI:

Atasay, A., Turemis, N. (2008). Effect of some nutrient applications on plant properties in organic strawberry production. Acta Hortic., 838, 83–86. DOI:

Benzie, I.F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal. Biochem., 239, 70–76. DOI:

Capocasa, F., Scalzo, J., Mezzetti, B., Battino, M. (2008). Combining quality and antioxidant attributes in strawberry: the role genotype. Food Chem., 111, 872–878. DOI:

Caglar, H., Paydas, S. (2002). Changes of quality characteristics and aroma compounds of hybrids and some strawberry cvs during harvest periods. Acta Hortic., 567, 203–206. DOI:

Cekic, C., Celebioglu, B, Ozturk Erdem, S. (2018). Effect of different strawberry pollinators on fruit properties of local Osmanli strawberry cultivar in Turkey. Appl. Ecol. Environ. Res., 16(5), 6845–6855.

Cemeroğlu, B. (2007). Food Analysis. Food Technology Publications, Ankara, 682 s.

Clifford, M.N.J. (2000). Review anthocyanins – nature, occurrence and dietary burden. J. Sci. Food Agric., 80, 1063–1072.;2-Q DOI:<1063::AID-JSFA605>3.0.CO;2-Q

Connar, A.M., Luby, J.J., Tong, C.B.S., Finn, C.E., Hancock, J.F. (2002). Variation and heritability estimates for antioxidant activity. Total phenolic content and anthocyanin content in blueberry progenies. J. Am. Soc. Hortic. Sci., 1, 82–88. DOI:

Copetti, C., Borges, G.S., Oliveira, J.L.B., Gonzaga, L.V., Fett, R., Bertoldi, F.C. (2012). Antioxidant properties and productivity of different strawberries cultivars Fragaria × ananassa Duch produced in an hydroponic system. Acta Hortic., 947, 369–337. DOI:

Erenoglu, B., Erbil, Y., Ufuk, S. (1998). Melezleme Yolu ile Çilek Islahı-1, Bilimsel Araştırma ve İncelemeler, 100 p.

Esitken, A., Alan, F. (2016). Studies on the determination of performance of some day neutral strawberry (Fragaria × ananassa) varieties in Kayseri conditions. Bahce 45(special ed. 2), 79–91.

Gecer, M.K., Gundogdu, M., Basar, G. (2018). Determining yield of some strawberry cultivars in ecology of Merzifon (Amasya). Iğdır Univ. J. Inst. Sci. Technol., 8(2), 6–12. DOI:

Giusti, M.M., Wrolstad, R.E. (2005). Characterization and measurement of anthocyanins by UV-visible spectroscopy. In: Handbook of food analytical chemistry, Wrolstad, R.E., S.J. Schwartz (eds). Wiley, New York, p. 19–31.

Gunduz, K. (2010). The effect of some strawberry cultivars cultivated in field and high tunnel in Amik Plain conditions to yield, quality and earliness [doctoral thesis]. Mustafa Kemal University Graduate School of Natural and Applied Sciences, Antakya.

Gunduz, K., Bayazit, S. (2017). Phenotypic variability in strawberry cultivars from different breeding program. J. Agric. Fac. Mustafa Kemal Univ., 22(2), 35–48

Hancock, J.F., Luby, J.J., 1993. Genetic resources at our doorstep: the wild strawberries. BioScience, 43, 141–147. DOI:

Karakaya, M., Ozturk, B., Islam, A., Karakaya, O., Kacar, E., Turga, E., Gun, S. (2015). Fruit quality characteristics of some strawberry varieties cultivated in Ordu ecological conditions. VII National Horticultural Congress, 25–29.08.2015, Çanakkale-Turkey.

Konarlı, O., Kepenek, K., Akgun, H. (1984). Melezleme Yolu ile Elde Edilen Yeni Çilek Çeşitleri. Bahçe, 13, 5–13.

Lopes da Silva, F., Escribano-Bailon, M.T., Alonso, J.J.P., Rivas-Gonzalo, J.C., Santos Buelga, C. (2007). Anthocyanin pigments in strawberry. LWT – Food Sci. Technol., 40 (2), 374–382. DOI:

Oguz, H.I., Kıroglu Zorlugenc, F., Kafkas, E. (2017). Determination of fruit quality characteristics of some strawberry (Fragaria x ananassa L.) varieties cultivated in nevşehir climate conditions. Bahçe, 46(special ed. 1), 303–310.

Oz, A.T., Eker, T. (2016). Determination of quality and phytochemical composition of Ottoman and Rubygem strawberry varieties grown in Osmaniye conditions. Bahçe, 46(special ed. 2), 195–199

Ozbahcali, G., Aslantaş, R. (2015). Some strawberry cultivars (Fragaria × ananassa Duch.) determination of performance in Erzurum ecological conditions. Atatürk Univ. J. Agric. Fac., 46(2), 75–84.

Ozgen, M., Reese, R.N., Tulio A.Z., Miller, A.R., Scheerens, J.C. (2006). Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2’-Diphenyl-1-picrylhydrazyl (DPPH) methods. J. Agric. Food Chem., 54, 1151–1157. DOI:

Ozgen, M., Serce, S., Gunduz., K., Yen, F., Kafkas, E., Paydas, S. (2007). Determining total phenolics and antioxidant activity of selected Fragaria genotypes. Asian J. Chem., 19, (7), 5573–5581.

Ozok, N. (2021). Pomological and morphological traits of some strawberry cultivars under Bursa ecological conditions. Uludağ University, Graduate School of Natural and Applied Sciences Department of Horticulture (master thesis), Bursa.

Oztürk Erdem, S., Cekic, C. (2017). Strawberry breeding from past to today. J. Gaziosmanpasa Sci. Res., 6(3), 105–115.

Ozturk Erdem, S. (2018). Osmanli strawberry breeding – I [doctoral thesis]. Tokat Gaziosmanpasa University Institute of Graduate Studies, Tokat, 196 p.

Oztürk Erdem, S., Cekic, C. (2020). Osmanli strawberry breeding – II. Bilecik University Edebali BAP Commission Project Final Report-2018-02.BŞEÜ.06-04

Sandra, M.H. (2004). Potential impact of strawberries on human health. A rewiev of the science. Crit. Rev. Food Sci. Nutr., 44, 1–17. DOI:

Saracoglu, O., Ozgen, M. (2015). The effect of different harvest period on fruit quality and phytochemical properties of short and day neutral strawberries. Turk. J. Agric. – Food Sci. Technol., 3(7), 545–549. DOI:

Serce, S. (2006). Scientific report on a short-term scientific mission. Forli-Italy, 7 pp.

Serce, S., Ozgen, M. (2014). Çilek Yetiştiriciliği ve Yeni Eğilimler. Tarım Türk Dergisi. Available: (12.01.2017).

Shaw, R.H. (1988). Climate requirement. In: Corn and corn 638 improvement, Sprague G.F., Dudly J.W. (eds). 3rd ed. Madism, WI, USA, 609. DOI:

Singleton, V.L. ve Rossi, J.L. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult., 16(3), 144–158.

Sistrunk, W.A., Morris, J.R. (1985). Strawberry quality: influence of cultural and environmental factors. In: Evaluation of quality of fruits and vegetables, Pattee, H.E. (ed.). AVI Pub., Westport, CT, 217–256. DOI:

Terefe, Y., Tafess, K., Fekadie, G., Kebede, N. (2013). Prevalence of lungworm infection in small ruminants in North Gondar zone, Amhara National Regional State, Ethiopia. J. Parasitol. Vector Biol., 5(4), 40–45. 13.0110

Ustun, P., Paydas, S. (1995). Bazı Melez Çilek Çeşit Adaylarının Verim ve Kalitesi Üzerinde Araştırmalar. Turkey II. National Horticultural Congress 3–6.11.1995, Adana, Turkey 301–305.

Yasa, N.E. (1997). Bazı Kültür Çilek Çeşitleri ile Melez Çilek Çeşit Adaylarının Demir (Fe) Klorozuna Dayanım Dereceleri ve Kromozom Sayılarının Saptanması Üzerine Araştırmalar [master thesis]. Cukurova University Institute of Natural and Applied Sciences, Adana.



Sinem Ozturk Erdem 
Department of Horticulture, Faculty of Agriculture and Natural Science, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey
Cetin Cekic 
Department of Horticulture, Faculty of Agriculture, Tokat Gaziosmanpasa University, 60240, Tasliciftlik, Tokat, Turkey
Davut Uysal 
Department of Horticulture, Faculty of Agriculture and Natural Science, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey


Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.


Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.