Interspecies hybridizations in situ with bovine heterosome painting probes for identification of sex chromosomes in fallow deer (Dama dama)
ANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówBARBARA DANIELAK-CZECH
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówMAREK BABICZ
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 LublinAGNIESZKA BĄK
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówBARBARA REJDUCH
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówAbstract
The phenomenon of syntenic genetic conservation of chromosomes enables application of molecular probes obtained from one species of animals to detect homologous DNA segments in another species. The aim of this study was identification of sex chromosomes in fallow deer (Dama dama), using in interspecies hybridizations in situ (Zoo-FISH technique) the bovine heterosomes painting probes. The results obtained showed distinct yellow-green signals in big acrocentric chromosomes X and strong red fluorescence signals in small submetacentric heterosomes Y in all fallow deer metaphase plates. The studies confirmed the high degree of genetic conservation of heterosome synteny groups in the species belonging to Ruminantia, which makes it possible to use of bovine, heterologous sex chromosomes painting probes in interspecies comparative, phylogenetic and evolutionary analyses.
Keywords:
Fallow deer (Dama dama), syntenic genetic conservatism, sex chromosomes, bovine heterosomes probes, Zoo-FISH techniqueReferences
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Słota E., Kozubska-Sobocińska A., Bugno M., Giemza-Marek A., Kulig B., 2001. Comparison between the G-banded karyotype of the aoudad (Ammotragus lervia) and sheep (Ovis aries). J. Appl. Genet. 42, 59–64.
Chi J., Fu B., Nie W., Wang J., Graphodatsky A.S., Yang F., 2005. New insights into the karyotypic relationships of Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis). Cytogenet. Genome Res. 108, 310–316.
Chowdhary B.P., Frönicke L., Gustavsson I., Sherthan H., 1996. Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping based chromosomal homologies. Mamm. Genome 7, 297–302.
Danielak-Czech B., Kozubska-Sobocińska A., Rejduch B., 2010. Diagnosis of tandem fusion translocation in the boar using FISH technique with human painting probes. Ann. Anim. Sci. 10, (4), 361–366.
Goldammer T., Brurmer R.M., Weikard R., Schwerin M., 1996. Generation and use of chromosome fragment specific bovine DNA probes for cytogenetic studies in cattle. Arch. Zoot. 45, 309–314.
Huang L., Nie W.H., Wang J.H., Su W.T., Yang F.T., 2005. Phylogenomic study of the subfamily Caprinae by cross-species chromosome paining with mantjac paints. Chromosome Res. 13, 389–399.
Iannuzzi L., Di Meo G.P., 1995. Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G- and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes. Chromosome Res. 3, 291–299.
Kozubska-Sobocińska A., Słota E., Pieńkowska A., 2003. Zastosowanie techniki FISH do diagnozy chimeryzmu leukocytarnego u owiec. (in Polish with English summary). Med. Wet. 59, 987–989.
Kozubska-Sobocińska A., Słota E., Pieńkowska-Schelling A., Schelling C., 2005. Comparative hybridization of the Y chromosome in selected species of Bovidae. Ann. Anim. Sci. 5 (1), 5–9.
Kozubska-Sobocińska A., Słota E., Pakusiewicz M., 2006. Comparison of the G-banded karyotype of the fallow deer (Dama dama) and sheep (Ovis aries). Ann. Anim. Sci. 6, 2, 225–231.
Kozubska-Sobocińska A., Ząbek T., Słota E., Kaczor U., 2007a. Comparison of GTG-banded karyotypes and microsatellite sequences in some species of the Bovidae and Cervidae families. J. Anim. Feed Sci., 16, 567–578
Kozubska-Sobocińska A., Rejduch B., Słota E., 2007b. Genetic conservatism analysis based on G-banded chromosomes of cattle and fallow deer. Ann. Anim. Sci. 7(2), 215–220.
Kozubska-Sobocińska A., Rejduch B., 2008. Identification of heterosomes in spermatozoa of rams with 54,XX/54,XY chimerism. Vet. Med.-Czech, 53(5), 250–254.
Kozubska-Sobocińska A., Rejduch B., Kaczor U., Sharan M., 2009a. Microsatellite sequences of Y heterosome. Anim. Biol. 11, (1–2), 247–250.
Kozubska-Sobocińska A., Bugno-Poniewierska M., Słota E., 2009b. Application of bovine heterosome painting probes to analysis of the sex bivalent in rams. Ann. Anim. Sci. 9, (4), 371–378.
Kozubska-Sobocińska A., Rejduch B., Danielak-Czech B., Babicz M., Bąk A., 2012. Comparative sex chromosomes hybridizations in Ruminantia. Ann. Anim. Sci. 12, (4), 497–502.
Oh S.H., Yun Y.M., Lee J.E., Kim I.Y., Shin J.H., Kweon O.K., Lee H., Yoon Y.S., Shin N.S., Seong J.K., 2011. G-,R- and C-band patterns of goral (Nemorhaedus caudatus) and comparison to goat (Capra hircus). Mol. Cells 31, 351–354.
Rejduch B., Kozubska-Sobocińska A., Radko A., Rychlik T., Słota E., 2004. The application of genetic markers for cell chimerism diagnosis in lambs. J. Anim. Breed. Genet. 121, 197–203.
Rejduch B., Danielak-Czech B., Kozubska-Sobocińska A., 2010a. FISH – based comparative analysis of human and porcine chromosome region involving obesity-related genes. Ann. Anim. Sci. 10, (4), 367–372.
Rejduch B., Kozubska-Sobocińska A., Danielak-Czech B., 2010b. Use of human painting probes for identification of centric fusion in wild boar. Chromosome Res. 18, 727–728.
Révay T., Tardy E.P., Tóth A., Kovács A., Salgó A., 2000. Sexing bovine cells by FISH with a synthetic Y-probe. 14th Europ. Colloq. Cytogenet. Domest. Anim. Abstr. Brno, 29.
Révay T., Kovacs A., Rens W., Gustavsson I., 2002. Simultaneous detection of viability and sex of bovine spermatozoa. Reprod. Fert. Dev. 14, 373–376.
Rubini M., Negri E., Fontana F., 1990. Standard karyotype and chromosomal evolution of the fallow deer (Dama dama L.). Cytobios 64, 155–161.
Słota E., Kozubska-Sobocińska A., Bugno M., Giemza-Marek A., Kulig B., 2001. Comparison between the G-banded karyotype of the aoudad (Ammotragus lervia) and sheep (Ovis aries). J. Appl. Genet. 42, 59–64.
ANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
BARBARA DANIELAK-CZECH
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
MAREK BABICZ
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
AGNIESZKA BĄK
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
BARBARA REJDUCH
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
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