Abstract
Virus-infected tulip bulbs produce flowers with virus symptoms what makes cut flowers unmarketable. The aim of the work was to check frequency of virus presence in bulbs intended for forcing and originating both from the Polish farms and imported from The Netherlands. The tests were carried out on four tulip cul-tivars– ‘Hermitage’, ‘Pretty Woman’, ‘Purple Prince’ and ‘Strong Gold’ during four forcing seasons be-tween 2010 and 2013. Bulb samples were tested by DAS-ELISA using antibodies specific for following vi-ruses: Tulip breaking, TBV, Tobacco necrosis, TNV, Lily symptomless, LSV, Cucumber mosaic, CMV, Tobacco rattle, TRV (J and F strains) and Tulip X, TVX. The most frequently detected virus, both in Polish and Dutch bulbs, was TBV (138 positive results among 1,360 tested samples, during four seasons, what means approx. 10 percent). However, TBV was detected predominantly in bulbs originating from the Polish plantations. Other viruses were detected sporadically. Our results lead to the conclusion that the selection done during tulip vegetation on the examinated Polish farms and removal of diseased plants was not effec-tive and chemical protection against aphids as TBV vectors may be insufficient. Consequently, many tulip bulb lots taken from the examined Polish plantations cannot meet the high quality level of Dutch bulbs, in particular in the case of TBV occurrence.
References
Beekwilder, J., van Houwelingen, A., van Beckhoven, J., Speksnijder, A. (2008). Stable recombinant alpaca antibodies for detection of Tulip virus X. Eur. J. Plant Pathol., 121, 477–485.
Buschman, J.C.M. (2005). Globalisation – flower – flower bulbs – bulb flowers. Acta Hort., 673, 27–33.
Clark, M.F., Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34, 475–483.
Clark, M.F., Lister, R.M., Bar-Joseph, M. (1988). ELISA Techniques. In: Methods for Plant Molecular Biology, Weissbaum, A., Weissbaum, H. (eds.). Acad. Press, 527.
Jabłońska, L. (2005). Rozwój polskiego kwiaciarstwa w minionym 15-leciu. Zesz. Probl. Post. Nauk Roln., 504, 21–31 (in Polish, with English abstract).
Jabłońska, L. (2007). Ekonomiczne aspekty rozwoju sektora kwiaciarskiego w Polsce. Wyd. SGGW, Warszawa (in Polish).
Jabłońska, L., Olewnicki, D., Ragan, M., (2013). Zachowania konsumenckie na warszawskim rynku kwiatów ciętych i doniczkowych. Zesz. Nauk. SGGW. Polit. Eu-rop., Fin. Market., 9(58), 220–230 (in Polish, with English abstract).
Kamińska, M., Śliwa, H., Malinowski, T. (2005). Partial characterisation of cucumber mosaic virus isolate infecting Loniceraca prifolium L. plants. Acta Sci. Pol. Hortorum Cultus, 4(2), 3–10.
Knippels, P.J.M. (2005). The contribution of quality inspections to the improvement of the quality of the Dutch flower bulbs and access to export markets. Acta Hort., 673, 79–84.
Knippels, P.J.M. (2011). Recent developments in the inspection schemes of flower bulbs. Acta Hort., 886, 147–151.
Mowat, W.P. (1995). Tulip. In: Virus and virus-like diseases of bulb and flower crops, Loebenstein, G., Law-son, R.H., Brunt, A.A. (eds.). John Wiley & Sons, Chichester, New York, Brisbone, Toronto, Singapore, 352–383.
Okubo, H., Sochacki, D. (2012). Botanical and horticultural aspects of major ornamental geophytes. In: Ornamental geophytes. From basic science to sustainable production, Kamenetsky, R. Okubo, H. (eds.). CRC Press. Taylor and Francis Group, Boca Raton – London – New York, 77–121.
Polder, G., van der Heijden, G.W.A.M., van Doorn, J., Baltissen, T.A.H.M.C. (2014). Automatic detection of tulip breaking virus (TBV) in tulip fields using machine vision. Biosyst. Eng., 117, 35–42.
Sochacki, D. (2013). The occurrence of the viruses in tulip crops in Poland. J. Hort. Res., 21(1), 5–9.
Sochacki, D., Komorowska, B. (2012). First report of Tulip virus X on tulip in Poland. Plant Dis., 96, 4, 594–594.
Ward, L.I., Tang, J., Quinn, B.D., Martin, E.J., Clover, G.R.G. (2008). First report of Tulip virus X in New Zealand. New Dis. Rep., 17, 22.
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