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Biology and epidemiology of Valdensinia heterodoxa Peyronel in Poland

DOI: https://doi.org/10.24326/asphc.2025.5525
Submitted: 14 April 2025
Published: 23.10.2025

Abstract

In Poland, the first description of the causal agent of the valdensia leaf blight fungus Valdensinia heterodoxa was on highbush blueberry in 2011. Our study identified this pathogen in highbush blueberry plantations and on bilberry plants to describe its biology, molecular characteristics, and disease epidemiology. The 115 fungus isolates yielded were divided into 2 groups based on the colony morphology differences on PDA (potato dextrose agar) medium. For the isolates from each group, morphology and growth dynamics were studied on PDA and WOA (weak oatmeal agar) with added highbush blueberry/bilberry/lily of the valley leaf decoction under different light and temperature conditions. The isolates’ colony morphology differences were noted depending on the medium type. The best isolate growth was at 20 °C/19 °C-day, 12 °C-night, with a 12-hour photoperiod. Isolate identification using PCR showed nearly 100% nucleotide sequence similarity to reference isolates. On infected leaves, the release height of conidia was 18 cm. The presented results are novel in Poland and have a broader-than-domestic significance.

References

  1. Aamlid, D. (2000). Infections of Valdensinia heterodoxa and Pucciniastrum vaccinii on bilberry (Vaccinium myrtillus). For. Pathol., 30, 135–139. https://doi.org/10.1046/j.1439-0329.2000.00194.x
  2. Abbasi, P.A., Hildebrand, P.D., Ali, S., Wright, H., Franklin, J., Renderos W.E. (2023). Effects of temperature and wet-ness on components of the infection cycle of Valdensia heterodoxa in lowbush blueberry. Plant Dis., 107, 167–176. https://doi.org/10.1094/PDIS-10-21-2248-RE
  3. Ali, S., Hildebrand, P.D., Renderos, W.E., Abbasi, P.A. (2021). Identification and characterization of Sphaerulina vac-cinia sp. nov. as the cause of leaf spot and stem canker in lowbush blueberry and its epidemiology. Phytopathology, 111(9), 1560–1570. https://doi.org/10.1094/PHYTO-04-20-0143-R
  4. Annis, S., Yarborough, D. (2009). Valdensinia leaf spot disease. The University of Mine–Cooperative Extension: Maine Wild Blueberries. Available online: http://umaine.edu/blueberries/factsheets/disease/valdensinia–leaf–spot–disease [date of access: 24.03.2014].
  5. Bavendamm, W. (1944). Valdensia heterodoxa, ein neuer Buchenschädling. Forstwissenschaftliches Centralblatt, 66, 54–60.
  6. Bryk, H., Michalecka, M., Poniatowska, A., Kałużna, M., Cieślińska, M. (2020). Integrowane metody ograniczania chorób [Integrated disease control methods]. In: H., Bryk (eds), Metodyka integrowanej ochrony borówki wysokiej [Methodology for integrated protection of highbush blueberries]. Instytut Ogrodnictwa, Skierniewice, 18–26.
  7. Cieślińska, M. (2020). First report of Blueberry mosaic associated virus in highbush blueberry in Poland. Plant Dis., 104(10), 2743. https://doi.org/10.1094/PDIS-03-20-0574-PDN
  8. Cornel, A., Percival, D. (2023). Evaluating fungicide efficacy for wild blueberry foliar disease complex management in eastern Canada. Acta Hortic., 1378, 113–120. https://doi.org/10.17660/ActaHortic.2023.1378.15 [date of access: 6.06.2024]
  9. Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19, 11–15.
  10. Dzięcioł, R., Mirzwa-Mróz, E., Zielińska, E., Wińska-Krysiak, M., Wakuliński, W. (2014). Valdensinia heterodoxa Pey-ronel as a new pathogen of blueberry in Poland. Plant Dis., 98(5), 688. https://doi.org/10.1094/PDIS-06-13-0644-PDN
  11. Farr, D.F., Rossman, A.Y. (2013). Fungal databases, systematic mycology and microbiology laboratory ARS, USDA. Available online: http://nt.ars-grin.gov/fungaldatabases/ [date of access: 6.06.2015].
  12. Gams, W., Van Der Aa, H.A., Van Der Plaats-Niterink, A.J., Samson, R.A., Stalpers, J.A. (1987). CBS Course of mycol-ogy, 3th ed. Centraalbureau Voor Schimmelcultures, Baarn, p. 105.
  13. Gardes, M., Bruns, T.D. (1993). ITS primers with enhanced specificity for basidiomycetes‐application to the identifica-tion of mycorrhizae and rusts. Mol. Ecol., 2, 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  14. Hildebrand, P.D., McRae K.B., Lu X. (2001). Factors affecting flower infection and disease severity of lowbush blueber-ry by Botrytis cinerea. Can. J. Plant Pathol., 23, 364–370. https://doi.org/10.1080/07060660109506957
  15. Hildebrand, P.D., Renderos, W.E. (2007). Valdensinia leaf spot (Valdensinia heterodoxa) of commercial lowbush blue-berry in Atlantic Canada: an emerging new threat. Can J. Plant Pathol., 29, 90.
  16. Hildebrand, P.D., Renderos, W.E. (2010). Valdensinia leaf spot of lowbush blueberry. Agriculture and Agri-Food Cana-da.Available online:www.gov.pe.ca/photos/original/af fact Valden.pdf. [date of access: 11.03.2015].
  17. Hildebrand, P.D., Renderos, W.E. (2012). Seasonal release of conidia of Valdensia heterodoxa and disease progress in lowbush blueberry. Can. J. Plant Pathol., 35, 273–278.
  18. Hildebrand, P.D., Renderos, W.E., Delbridge, R.W. (2016). Diseases of lowbush blueberry and their identification. Agri-culture and Agri-Food Canada, Ottawa, p. 44. Available online: https://publications.gc.ca/collections/collection_2016/aac-aafc/A59-37-2016-eng.pdf [date of access: 13.05.2024].
  19. Hildebrand, P.D., Renderos, W.E., Fillmore, S.A., Walker, B.A. (2011). Susceptibility of lowbush blueberry in the sprout and crop phase to Valdensinia leaf spot. Can. J. Plant Pathol., 33, 568.
  20. Holst-Jensen, A., Kohn, L.M., Schumacher, T. (1997). Nuclear rDNA phylogeny of the Sclerotiniaceae. Mycologia, 89, 885–899. https://doi.org/10.1080/00275514.1997.12026859
  21. https://blast.ncbi.nlm.nih.gov/Blast.cgi
  22. Kałużna, M., Pothier, J.F. (2022). Complete genome sequence data of two Xanthomonas arboricola strains isolated from blueberry plants displaying bacterial leaf blight in Poland. Phytopathology, 112,8, 1814–1818. https://doi.org/10.1094/PHYTO-11-21-0484-A
  23. Kałużna, M., Puławska, J., Meszka, B. (2013). A new bacterial disease on blueberry (Vaccinium corymbosum) caused by Pseudomonas spp. J. Plant Prot. Res., 53(1), 32–36.
  24. Kirk, P.M., Cannon, P.F., Minter, D.W., Stalpers, J.A. (2008). Ainsworth & Bisby’s dictionary of the fungi. 10th ed. CABI Europe–UK, Egham and CAB International, Wallingford United Kingdom, 623–624, 719.
  25. Krysicki W., Bartos J., Dyczka, W., Królikowska, K., Wasilewski, M. (1994). Rachunek prawdopodobieństwa i stat-ystyka matematyczna w zadaniach. Cz. II. Statystyka matematyczna [Probability theory and mathematical statistics in tasks. Part II. Mathematical statistics]. Wydawnictwo Naukowe PWN, Warszawa, p. 325.
  26. Kukuła, W., Mirzwa-Mróz, E., Wakuliński, W., Paduch-Cichal, E. (2017). Characteristics of Valdensinia heterodoxa Peyr. as an Ericaceae pathogen in Poland. Acta Sci. Pol. Hortorum Cultus, 16(4), 101–112. https://doi.org/10.24326/asphc.2017.4.11
  27. Larena, I., Salazar, O., Gonzales, V., Julian, M.C., Rubio, V. (1999). Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes. J. Biotech., 75, 187–194.
  28. Lyon, E. (2015). Dispersal and establishment of the fungal pathogen, Valdensia heterodoxa, in Maine [M. Sc Thesis]. The University of Maine, Orono, ME. Available online: https://digitalcommons.library.umaine.edu/etd/2382 [date of ac-cess: 10.06.2024].
  29. Magnussen, S., Vogelgsang, S., Shamoun, S.F. (2004). Nonlinear mixed models for repeated data assessment of time and temperature effects on conidia production in the fungus Valdensinia heterodoxa. Biocontrol, 49, 47–62. https://doi.org/10.1023/B:BICO.0000009376.53688.8e
  30. Marcinkowska, J. (2012). Oznaczanie rodzajów grzybów sensu lato ważnych w fitopatologii [Identification of fungal species important in plant pathology]. Wydawnictwo Rolnicze i Leśne PWRiL, Warsaw, Poland, p. 508.
  31. Melnik, V.A. (1981). Valdensia heterodoxa Peyronel, an unusual but very common Hyphomycetes [Fungi]. Novosti siste-matiki nizshikh rastenii – Akademiia Nauk SSSR, Botanicheskii Institut Petersburg, Russia, 18, 96–99.
  32. Melnik, V.A. (2004). Parasitica hyphomycete Valdensia heterodoxa on Rubus saxatilis in Leningrad oblast. Mikol. Fitopatol., 28, 43–45.
  33. Melnik, V.A., Popov, E.S., Shabunin, D.A. (2007). Contributions to the studies of mycobiota in Novgorod and Pskov regions. Hyphomycetes. Mikol Fitopatol., 41, 515–525.
  34. Meszka, B., Bielenin, A. (2012). Antraknoza borówki wysokiej, występowanie, szkodliwość i możliwości zwalczania [Blueberry anthracnose, occurrence, harmfulness and control possibilities]. Prog. Plant Prot., 52(1), 88–91.
  35. Mirzwa-Mróz, E., Szyndel, M.S., Wdowiak, M., Wit, M., Paduch-Cichal, E., Wilkos, A., Felczak-Konarska, K., Wakuliński W. (2023). Phenotypic characterization and phylogeny of Godronia myrtilli (Anamorph: Topospora myr-tilli) – causal agent of Godronia canker on highbush blueberry. Pathogens, 12(5), 1–11. https://doi.org/10.3390/pathogens12050642.
  36. Mułenko, W., Woodward, S. (1996). Plant parasitic hyphomycetes new to Britain. Mycologist, 10, 69–72.
  37. Nekoduka, S., Kanematsu, S., Tanaka, K., Harada, Y., Sano, T. (2012). Valdensinia leaf blight of blueberry caused by Valdensinia heterodoxa, a new fungal disease in Japan. J. Gen. Plant. Pathol., 78(3), 151–159. https://doi.org/10.1007/s10327-012-0373-y
  38. Nordin, A., Nasholm, T., Ericson, L. (1998). Effects of simulated N deposition on understory vegetation of a boreal coniferous forest. Ecology, 12, 691–699. https://doi.org/10.1046/j.1365-2435.1998.00240.x
  39. Norvell, L.L., Redhead, S.A. (1994). Valdensinia heterodoxa (Sclerotiniaceae) in the United States. Can. J. For. Res., 24, 1981–1983. https://doi.org/10.1139/x94-253
  40. Paduch-Cichal, E., Kalinowska, E., Chodorska, M., Sala-Rejczak, K., Nowak, B. (2011). Detection and identification of viruses of highbush blueberry and cranberry using serological ELISA test and PCR technique. Acta Sci. Pol., Horto-rum Cultus, 10(4), 201–215.
  41. Peyronel, B. (1923). Sopra un singolare parassita polifago: Valdensia heterodoxa n. gen et n. sp. [On a singular polypha-gous parasite: Valdensia heterodoxa n. gen. et n. sp.]. Stazione Sperimentale di Agrumicoltura Italiane, 56, 521–538.
  42. Peyronel, B. (1953). La forma ascofora di Valdensia heterodoxa Peyronel, tipo di un nuovo genere di Sclerotiniacee: Valdensinia. Nuovo Giornale Botanico Italiane, 59, 181–185.
  43. Redhead, S.A., Perrin, P.W. (1972a). Asterobolus: a new parasitic hyphomycete with a novel dispersal mechanism. Can. J. Bot., 50, 409–412.
  44. Redhead, S.A., Perrin, P.W. (1972b). Asterobolus: a synonym of Valdensia. Can. J. Bot., 50, 2083–2084.
  45. Siemaszko, W. (1929). Phytopathologische Beobachtungen in Polen. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg., 78, 113–116.
  46. Siemaszko, W. (1934). Zagadnienia zasięgów geograficznych chorób roślin uprawnych [Issues regarding the geographical range of crop diseases]. Rocz. Nauk Ogrod., 1, 163–170.
  47. Strengbom, J., Nordin, A., Nasholm, T., Ericson, L. (2002). Parasitic fungus mediates change in nitrogen-exposed boreal forest vegetation. J. Ecol., 90, 61–67. https://doi.org/10.1046/j.0022-0477.2001.00629.x
  48. Szmagara, M. (2009). Biodiversity of fungi inhabiting the highbush blueberry stems. Acta Sci. Pol. Hortorum Cultus, 8(1), 37–50.
  49. Vogelgsang, S., Shamoun, S.F. (2002). Growth, sporulation, and conidia discharge of Valdensinia heterodoxa, a foliar pathogen of salal, as influenced by temperature and photoperiod in vitro. Mycol. Res., 106, 480–490. https://doi.org/10.1017/S0953756202005737
  50. Vogelgsang, S., Shamoun, S.F. (2004). Evaluation of an inoculum production and delivery technique for Valdensinia heterodoxa, a potential biological control agent for salal. Biocontrol Sci. Techn., 14, 747–756. https://doi.org/10.1080/09583150410001720725
  51. White, T.J., Bruns, T., Lee, S., Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A., Innis, D.H., Gelfand, J.J. Sninsky, T.J. White (eds), PCR Protocols: a guide to methods and applications. Academic Press Inc., 315–322.
  52. Wilkin, J.E. (2004). Genetic diversity and population structure of the potential biocontrol agent, Valdensinia heterodoxa, and its host Gaultheria shallon (salal) [M.Sc. Thesis]. Department of Forest Sciences, The University of British Co-lumbia, Vancouver, B.C., Canada, 6–7.
  53. Wilkin, J.E., Shamoun, S.F., Ritland, C., Ritland, K., El-Kassaby, Y.A. (2005a). Population genetics of Gaultheria shal-lon in British Columbia and the implications for management using biocontrol. Can. J. Bot., 83, 501–509. https://doi.org/10.1139/b05-039
  54. Wilkin, J.E., Ritland, C., Ritland, K., El-Kassaby, Y.A. (2005b). Genetic diversity and population structure of Valdensin-ia heterodoxa, a potential biocontrol agent for salal in coastal British Columbia. Can. J. Plant Pathol., 27, 559–571. https://doi.org/10.1080/07060660509507257
  55. Zalewska, E., Król, E., Machowicz-Stefaniak, Z. (2007). Grzyby zasiedlające owoce borówki wysokiej (Vaccinium co-rymbosum L.) uprawianej w południowo-wschodniej Polsce [Fungi colonizing the fruits of blueberry (Vaccinium co-rymbosum L.) cultivated in south-eastern Poland]. Prog. Plant Prot., 47(2), 380–384.
  56. Zhao, S., Shamoun, S.F. (2006). The effects of culture media, solid substrates, and relative humidity on growth, sporula-tion and conidial discharge of Valdensinia heterodoxa. Mycol. Res., 110, 1340–1346. https://doi.org/10.1016/j.mycres.2006.08.001
  57. Zhao, S., Shamoun, S.F. (2010). Conidial morphology, structure and development in Valdensinia heterodoxa. Mycology, 1, 113–120. https://doi.org/10.1080/21501201003720665

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