ANTIFUNGAL ACTIVITY OF NANOPARTICLES AGAINST CHOSEN FUNGAL PATHOGENS OF CARAWAY
Ewa Dorota Zalewska
University of Life Sciences in LublinZofia Machowicz-Stefaniak
University of Life Sciences in LublinEwa Dorota Król
University of Life Sciences in LublinAbstract
The paper shows the first study on the effect of nanoparticles against fungal pathogens of caraway. The fertilizers containing nanoparticles of copper, silver, and chitosan: Viflo® copper, Viflo Chitosol® Silver® Viflo Cal S and isolates KLM 1806 and KLM 1813 of Septoria carvi were used to the study. The percent of growth inhibition, development of S. carvi colonies and ability of Viflo® Cal to control plants against infection by S. carvi was adopted as the criterion of evaluation of the nanoformulations. The field experiment evaluated the effect of Viflo® Cal S to plant health in terms of the occurrence of S. carvi and Erysiphe heraclei, colonization of plant tissues by S. carvi and the yield of schizocarps. In vitro it has been shown that the tested formulations with nanoparticles limited the growth and development of the S. carvi and Viflo Cal® S at the concentration of 1 g·cm-3 was the inhibitor of mycelial growth. In climatic chamber Viflo Cal® S protect caraway’s seedlings against the infection by S. carvi. In vivo Viflo Cal S® significantly limited the severity of septoriosis and powdery mildew, which contributed to increase the size and quality of the caraway crop.
Keywords:
caraway, fungi, control, nanopreparationsReferences
Feng, Q.L., Wu, J., Cheng, G.O., Cui, F.Z., Kim, T.N., Kim, J.O. (2000). A mechanic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater. Res., 52, 662–668.
Grzybowska, T., Kapała, H., Kwaśna, H. (1975). Choroby występujące w początkowym okresie wegetacji roślin zielarskich. Wiad. Zielar., 5, 3–4.
Hwang, E.T., Lee, J.H., Chae, Y.J., Kim, Y.S., Kim, B.C., Sang, B.I., Gu, M.B. (2008). Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria. Small, 4, 746–750.
Jampílek, J., Kráľová, K. (2015). Application of nanotechnology in agriculture and food industry, its prospects and risk. Ecol. Chem. Eng. S., 22(3), 321–361. doi: 10.1515/eces-2015-0018
Jo, Y.-K, Kim, B.H., Jung, G. (2009). Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi. Plant Dis., 93(10), 1037–1043. doi: 10.1094/PDIS-93-10-1037
Jung, W.K., Koo, H.Ch., Kim, K.W., Shin. S., Kim, S.H., Park, Y.H. (2008). Antibacterial activity and mechanism of action of sliver ion in Staphyllococcus aureus and Escherichia coli. Appl. Environ. Microbiol., 74(7), 2171–2178.
Kaur, P., Thakur, R., Choudhary, A. 2012. An in vitro study of the antifungal activity of silver/chitosan nanoformulations against important seed borne pathogens. IJSTR, 1(6), 83–86.
Kim, S.W., Jung J.H., Lamsal, K., Kim, Y.S., Min, J.S., Lee, Y.S. (2012). Antifungal effect of silver nanoparticles (AgNPs) against various plant pathogenic fungi. Mycobiology, 40(1), 53–58. doi.org/10.5941/MYCO.2012.40.1.053
Lamsal, K., Kim, S.W., Jung, J.H., Kim, Y.S., Kim, K.S., Lee, Y.S. (2011). Inhibition effects of silver nanoparticles against powdery mildews on cucumber and pumpkin. Mycobiology, 39(1), 26–32. doi: 10.4489/MYCO.2011.39.1.026
Mačkinaitė, R. (2012). Potential pathogens of common caraway (Carum carvi L.) seeds and search for measure suppressing their spread. Žemdirbyste = Agriculture, 99(2), 179–188.
Machowicz-Stefaniak, Z. (2010). Occurrence and characterization of Colletotrichum dematium (Fr.) Grove. Pol. J. Microbiol., 59(3), 191–200.
Machowicz-Stefaniak, Z., Zalewska, E. (2008). Biodiversity of fungi inhabiting various parts of caraway (Carum carvi L.). EJPAU, Horticulturae, 11(1), # 21. http://www.ejpau.media.pl/volume11/issue1/art.-21.html
Machowicz-Stefaniak, Z., Zalewska, E. (2011). Occurrence of Colletotrichum dematium on selected herbs species and preparations inhabiting pathogen’s growth and development in vitro. Ecol. Chem. Eng. S, 18(4), 465–478.
Mazur, S., Nawrocki, J. (2004). Fungal diseases threat on caraway plantations in south region of Poland. In: Proceeding of the XVI Slovak and Czech Plant Protection Conference. Acta Fytotechn. Zootechn., 7, 201–203.
Min, J.S., Kim, K.S., Kim, S.W., Jung, J.H., Lamsal, K., Kim, S.B., Jung, M., Lee, Y.S. (2009). Effect of colloidal silver nanoparticles on sclerotium-forming pathogenic fungi. Plant Pathol. J., 25(4), 376–380.
Odstrčilovà, L. (2007). Changes in the occurrence of mycoflora on caraway seeds after fungicide application. Plant Prot. Sci., 43(4), 146–150.
Odstrčilovà, L., Ondŕej, M., Kocourkovà, B., Růžičkovà, G. (2002). Monitoring of incidence and determination of chemical protection. Proc. 6th Conf. EFPP. Praha. Plant Prot. Sci., 38(Spec. Iss. 2), 340–343.
Ogar, A., Tylko, G., Turnau, K. (2015). Antifungal properties of silver nanoparticles against indoor mould growth. Sci. Total Environ., 521–522, 305–314. .doi.org/10.1016/j.scitotenv.2015.03.101
Thomson, J., Waterer, D. (2007). Evaluation of foliar fungicides for control of blossom blight in coriander and caraway. http://www.usask.ca/agriculture/plantsci/vegetable/med/mcumin.htm#FoliarFung
Thurman, K.G., Gerba, C.H.P., Bitton, G. (1989). The molecular mechanisms of copper and silver ion disinfection of bacteria and viruses. Crit. Rev. Environ. Sci. Technol., 18, 295–315.
Zalewska, E. (2005). Oddziaływanie wybranych fungicydów na wzrost i zarodnikowanie Monilia coryli (Schellenb.) in vitro. Annales UMCS, Sect. EEE, Horticultura, 15, 119–126.
Zalewska, E. (2013). Pathogenicity of Septoria carvi Syd. towards caraway Carum carvi L. (Apiaceae). J. Agr. Sci. Tech. A., 3, 711–723.
Zalewska, E.D. (2016). Harmfulness of Septoria carvi Syd. towards caraway and preparations limiting the growth and occurrence of the fungus. Ecol. Chem. Eng. S, (in print).
Zalewska, E.D., Machowicz-Stefaniak, Z., Król, E.D. (2015). Fungi colonizing caraway (Carum carvi L.) in different regions of cultivation. Acta Sci. Pol. Hortorum Cultus, 14(6), 175–188. doi: 10.13140/RG.2.1.1939.8485
http://www.weatheronline.pl/
University of Life Sciences in Lublin
University of Life Sciences in Lublin
University of Life Sciences in Lublin
License
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.
