In vitro REGENERATION INDUCED IN LEAF EXPLANTS OF Citrus limon L. Burm cv. ‘Primofiore’
Anna Kasprzyk-Pawelec
Maria Curie-Skłodowska University in LublinJacek Pietrusiewicz
Maria Curie-Skłodowska University in LublinEwa Szczuka
Maria Curie-Skłodowska University in LublinAbstract
In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon.
Keywords:
Citrus limon, phytohormones, explants, callus induction, auxin, cytokinin, 6-benzyloaminopurineReferences
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Carimi, F., De Pasquale, F., Crescimanno, F.G. (1994). Somatic embryogenesis from styles of lemon (Citrus limon L. Burm). Plant Cell Tiss. Org. Cult., 37, 209–211.
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Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15, 473–497.
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Rattanpal, H.S., Kaur, G., Gupta, M. (2011). In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) by direct organogenesis. Afr. J. Biotech., 10(63), 13724–13728.
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Singh, B., Kaur, A. (2011). Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants. J. Crop Sci. Biotech., 14(4), 297–303.
Singh, S., Rajam, M.V. (2009). Citrus biotechnology: Achievements, limitations and future directions. Physiol. Mol. Biol. Plants, 15(1), 3–22.
Singh, S., Ray, B.K., Bhattacharyya, S., Deka, P.C. (1994). In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm. f. HortSci., 29(3), 214–216.
Tusa, N., Grosser, J.W., Gmitter, F.G. (1990). Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon and the interspecific somatic hybrid following protoplasm fusion. J. Amer. Soc. Hort. Sci., 115(6), 1043–1046.
Yaacob, J.S., Mahmad, N., Taha, R.M., Mohamed, N., Yussof, A.I.M., Saleh, A. (2014). Optimization of culture conditions (sucrose, pH, and photoperiod) for in vitro regeneration and early detection of somaclonal variation in ginger lime (Citrus assamensis). The Sci. World J., Article ID 262710, 1–9.
Almeida, W.A.B., Mourão Filho, F.A.A., Mendes, B.M.J., Rodriguez, A.P.M. (2002). In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia. Sci. Agric., 59, 35–40.
Ashok Kumar, K., Narayani, M., Subanthini, A., Jayakumar, M. (2011). Antimicrobial activity and phytochemical analysis of citrus fruit peels – utilization of fruit waste. Internat. J. Engin. Sci. Technol., 3(6), 5414–5421.
Bansode, D.S., Chavan, M.D. (2012). Studies on antimicrobial activity and phytochemical analysis of Citrus fruit juices against selected enteric pathogens. Internat. Res. J. Pharm., 3(11), 122–126.
Benabdesselam, F.M., Khettal, B., Bedjou, F. (2011). Micropropagation of Algerian juvenile rootstocks Citrus species. Life Sci. Leaf., 18, 707–717.
Carimi, F., De Pasquale, F., Crescimanno, F.G. (1994). Somatic embryogenesis from styles of lemon (Citrus limon L. Burm). Plant Cell Tiss. Org. Cult., 37, 209–211.
Dhanavade, M.J., Jalkute, C.B., Ghosh, J.S., Sonawane, K.D. (2011). Study antimicrobial activity of lemon (Citrus lemon L.) peel extract. Brit. J. Pharm. Toxic., 2(3), 119–122.
FAO (2001). http://apps.fao.org/lim500/nph-wrap.pl.
Foschi, R., Pelucchi, C., Dal Maso, L., Rossi, M., Levi, F., Talamini, R., Bosetti, C., Negri, E., Serraino, D., Giacosa, A., Franceschi, S., La Vecchia, C. (2010). Citrus fruit and cancer risk in a network of case-control studies. Canc. Caus. Contr., 21(2), 237–242.
Gaurav, K., Richa, S. (2012). In vitro micropropagation of different species of citrus. Res. J. Biotech., 7(4), 96–101.
Kalinina, A., Brown, D.C.W. (2007). Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator. Plant Cell Rep., 26, 927–935.
Lombardo, G., Alessandro, R., Scialabba, A., Sciandra, M., De Pasquale, F. (2011). Direct organogenesis from cotyledons in cultivars of Citrus clementina Hort. Ex Tan. Am. J. Plant Sci., 2, 237–244.
Mukhtar, R., Khan, M.M., Fatima, B., Abbas, M., Shahid, A. (2005a). In vitro regeneration and multiple shoots induction in Citrus reticulata (Blanco). Int. J. Agri. Biol., 7(3), 414–416.
Mukhtar, R., Khan, M.M., Rafiq, R., Shahid, A., Khan, F.A. (2005b). In vitro regeneration and somatic embryogenesis in (Citrus aurantifolia and Citrus sinensis). Int. J. Agri. Biol., 7(3), 518–520.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15, 473–497.
Murashige, T., Tucker, D.P.H. (1969). Growth factor requirements of citrus tissue culture. Internat. Citrus Symp., 1, 1155–1169.
Rattanpal, H.S., Kaur, G., Gupta, M. (2011). In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) by direct organogenesis. Afr. J. Biotech., 10(63), 13724–13728.
Saini, H.K., Gill, M.S., Gill, M.I.S. (2010). Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lush.). Ind. J. Biotech., 9, 419:423.
Sarma, C., Borthakur, A., Singh, S., Modi, M.K., Sen, P. (2011). Efficient in vitro plant regeneration from cotyledonary explants of Citrus reticulata L. Blanco. Ann. Biol. Res., 2(6), 341–348.
Savita, Singh, B., Virk G.S., Nagpal A.K. (2011). An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush. Physiol. Mol. Biol. Plants, 17(2), 161–169.
Savita, Vijay, Virk, G.S., Nagpal, A. (2010). Effect of explant type and different plant growth regulators on callus induction and plantlet regeneration in Citrus jambhiri Lush. Environ. We Int. J. Sci. Tech., 5, 97–106.
Singh, B., Kaur, A. (2011). Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants. J. Crop Sci. Biotech., 14(4), 297–303.
Singh, S., Rajam, M.V. (2009). Citrus biotechnology: Achievements, limitations and future directions. Physiol. Mol. Biol. Plants, 15(1), 3–22.
Singh, S., Ray, B.K., Bhattacharyya, S., Deka, P.C. (1994). In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm. f. HortSci., 29(3), 214–216.
Tusa, N., Grosser, J.W., Gmitter, F.G. (1990). Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon and the interspecific somatic hybrid following protoplasm fusion. J. Amer. Soc. Hort. Sci., 115(6), 1043–1046.
Yaacob, J.S., Mahmad, N., Taha, R.M., Mohamed, N., Yussof, A.I.M., Saleh, A. (2014). Optimization of culture conditions (sucrose, pH, and photoperiod) for in vitro regeneration and early detection of somaclonal variation in ginger lime (Citrus assamensis). The Sci. World J., Article ID 262710, 1–9.
Anna Kasprzyk-Pawelec
Maria Curie-Skłodowska University in Lublin
Maria Curie-Skłodowska University in Lublin
Jacek Pietrusiewicz
Maria Curie-Skłodowska University in Lublin
Maria Curie-Skłodowska University in Lublin
Ewa Szczuka
Maria Curie-Skłodowska University in Lublin
Maria Curie-Skłodowska University in Lublin
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