Jacek Gawroński

University of Life Sciences in Lublin

Jerzy Hortyński

University of Life Sciences in Lublin

Elżbieta Kaczmarska

University of Life Sciences in Lublin

Magdalena Dyduch-Siemińska

University of Life Sciences in Lublin

Wojciech Marecki

University of Life Sciences in Lublin

Agata Witorożec

University of Life Sciences in Lublin


Blue honeysuckle due to the high health benefits of its fruit, early ripening (before the first strawberries cultivars) and high frost resistance of both plants and flowers has gained the great popularity both in cultivation and breeding. The implementation of the breeding program of new cultivars requires the knowledge of the characteristics that determine its direction in the selected material. Therefore, in this study the evaluation of the phenological phases, yield, fruit weight, number of seeds per fruit and pollen viability in the cultivars and breeding clones of this species was made. The tested cultivars and clones had different levels of the analysed qualities except the pollen viability which was high but did not differ significantly within the genotypes. The evaluation of such characteristics
as yield potential and fruit weight indicates that cultivars ‘Warszawa’, ‘Wojtek’ and T2 clone which had higher values of these characteristics as compared to other genotypes are possible to be used in breeding programme. Obtainment of the forms with early fruit ripening can be realised through the use of the selected Russian cultivars. The objective of this study was also to characterise the blue honeysuckle germplasms using RAPD markers and to assess their genetic similarity. The analysed primers produced 61 fragments out of which 57 (93.44%) were polymorphic. The genetic similarity matrix was made on the basis of RAPD markers. The mean genetic similarity was calculated at 0.56. The presented study confirms that the use of RAPD markers is a practical and effective method to evaluate the genetic similarity of blue honeysuckle genotypes and to establish genetic relationships between these genotypes.


blue honeyberry, Random Amplified Polymorphic DNA (RAPD), genetic variability, pollen viability, flowering and ripening period, yielding

Arus L., Kask K. 2007. Edible honeysuckle (Lonicera caerulea var. edulis) – underutilized berry crop in Estonia. N.J.R. Report., 3(1), 33–36.
Bors B., 2009a. Blue honeysuckle. http://www.fruit.usask.ca/articles/blue_honeysuckle2.pdf.
Bors B., 2009b. Comparing Haskap to other Berries. http: //www. fruit.usask.ca/articles /Haskap/Haskap Sister crops.pdf.
Bożek M., Wieniarska J., 2006. Biologia kwitnienia i wydajność cukrowa kwiatów dwóch odmian Lonicera kamtschatica (Sevast.) Pojark. Acta Agrobot., 59, 177–182.
Doyle J.J., Doyle J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19, 11–1.
Frejnagel S., 2007. Comparison of polyphenolic composition of extracts from honeysuckle, chokeberries and green tea – a short report. Pol. J. Food Nutr. Sci., 57(1), 83–86.
Fu J., Yang L., Khan A., Mei Z., 2013. Genetic characterization and authentication of Lonicera japonica Thunb. by using improved RAPD analysis. Mol. Biol. Rep., 40(10), 5993–5999.
Golis A., Korbin M., Pluta S., Zurawicz E., 2001. Badania nad otrzymywaniem mieszańców oddalonych w obrębie rodzaju Ribes. Folia Hort. Ann., 13/1A, 473–479.
Golis T., 2007. Jagoda kamczacka cenna roślina do uprawy towarowej. Sad Nowocz., 10, 25–27.
Gruia M.I., Oprea E., Gruia I., Negoita V., Farcasanu I.C., 2008. The antioxidant response induced by Lonicera caerulaea berry extracts in animals bearing experimental solid tumors. Molecules, 13, 1195 – 1206.
Gwozdecki J., Golis T., 2006. Ocena odmian suchodrzewu jadalnego (Lonicera sp.). XLIV Ogólnopolska Naukowa Konferencja Sadownicza, Skierniewice, 30–31 sierpnia, 74–75.
Hummer K.E., 2006. Blue honeysuckle: a new berry crop for North America. J. Amer. Pomol. Soc., 60(1), 3–8.
Huo J., Dong Q., 2012. RAPD analysis of the genetic diversity of wild blue honeysuckle (Lonicera caerulea) in northeast China. Acta Hort., 935, 185–189.
Jurikova T., Rop O., Mlcek J., Sochor J., Balla S., Szekeres L., Hegedusova A., Hubalek J., Adam V., Kizek R., 2012. Phenolic profile of edible honeysuckle berries (Genus Lonicera) and their biological effects. Molecules, 17, 61–79.
Kaczmarska E., 2009. Growth, flowering and yielding of six American cranberry (Vaccinium macrocarpon Ait.) cultivars. Acta Sci. Pol., Hortorum Cultus, 8(4), 35–44.
Kaczmarska E., Dobrowolska A.M., Hortyński J.A., 2008. The influence of pollen viability on seed set and fruit mass in strawberry (Fragaria × ananassa Duch.). Acta Agrobot., 61(1), 79–84.
Lamoureux D., Sorokin A., Lefevre I., Alexanian S., Eyzaguirre P., Hausman J.F., 2011. Investigation of genetic diversity in Russian collections of raspberry and blue honeysuckle. Plant Genet. Res., 9(2), 202–205.
Małodobry M., Bieniasz M., Dziedzic E., 2008. Ocena plonowania oraz zawartości niektórych składników w owocach dwóch odmian suchodrzewu sinego. XLV Ogólnopolska Naukowa Konferencja Sadownicza, Skierniewice, 28–29 sierpnia, 94–96.
Małodobry M., Bieniasz M., Dziedzic E., 2010. Evaluation of the field and some components in the fruit of blue honeysuckle (Lonicera caerulea var. edulis Turcz. Freyn.). Folia Hort. Ann., 22/1, 45–50.
Miyashita T., Hoshino Y., 2010. Interspecific hybridization in Lonicera caerulea and Lonicera gracilipes: the occurrence of green/albino plants by reciprocal crossing. Sci. Hort., 125, 692–699.
Miyashita T., Ohashi, T., Shibata F., Araki H., Hoshino Y., 2009. Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx. Plant Cell Tiss. Organ. Cult., 98(3), 291–301.
Naugžemys D., Žilinskaitė S., Denkovskij J., Patamsytė J., Literskis J., Žvingila D. 2007. RAPD based study of genetic variation and relationships among Lonicera germplasm accessions. Biologija, 53(3), 34–39.
Naugžemys D., Žilinskaitė S., KleizaitėV., Skridaila A., Žvingila D. 2011. Assessment of genetic variation among elite and wild germplasm of blue honeysuckle (Lonicera caerulea L). Baltic Forest., 17(32), 8–16.
Nei M., Li W.H., 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad Sci., 76, 5269–5273.
Ochmian I., Grajkowski J., 2007. Wzrost i plonowanie trzech odmian jagody kamczackiej (Lonicera caerulea) na Pomorzu Zachodnim w pierwszych latach po posadzeniu. Rocz. AR Pozn. 383, Ogrodnictwo, 41, 351–355.
Ochmian I., Grajkowski J., Skupień K., 2008. Field performance, fruit chemical composition and firmness under cold storage and simulated “shelf-life” conditions of three blue honeysuckle cultigenes (Lonicera caerulea). J. Fruit Ornam. Plant Res., 16, 83–91.
Ochmian I., Smolik M., Dobrowolska A., Rozwarski R., Kozos K., Chełpiński P., Ostrowska K., 2013. The influence of harvest date on fruit quality of several cultivars of blue honeysuckle berries. EJPAU, Horticulture, 16, 1, 2, http://www.ejpau.media.pl.
Pierzga K., 2001. Suchodrzew jadalny. Szkółkarstwo 5, 14–15.
Plekhanova M.N., 2000. Blue honeysuckle (Lonicera caerulea L.) a new commercial berry crop for temperate climate: genetic resources and breeding. Acta Hort., 538, 159–164.
Rohlf F.J., 2001. NTSYS-pc numerical taxonomy and multivariate analysis system. Version 5.1. Exeter Publishing Ltd., Setauket, N.Y.
Skowera B., Puła J., 2004. Skrajne warunki pluwiotermiczne w okresie wiosennym na obszarze Polski w latach 1971–2000. Acta Agroph., 3(1),171–177.
Skupień K., Ochmian I., Grajkowski J., 2009. Influence of ripening time on fruit chemical composition of two blue honeysuckle cultigens. J. Fruit Ornam. Plant Res., 17(1), 101–111.
Smolik M., Ochmian I., Grajkowski J., 2010. Genetic variability of Polish and Russian accessions of cultivated blue honeysuckle (Lonicera caerulea). Plant Gen., 46(8), 960–966.
Sun F., Huo J., Qin D., 2011. Establishment and optimization of SRAP amplification system in Lonicara caerulea L. J. Northeast Agricult. Univ. (English ed.), 18(4), 26 – 31.
Svarcova I., Heinrich J., Valentova K., 2007. Berry fruits as a source of biologically active compounds: The case of Lonicera caerulea. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 151(2), 163–174.
Szot I., Lipa T., 2013. Estimating the fruit quality after application the pruning of blue honeysuckle bushes. Modern Phytomorphology 4, 51–54.
Szot I., Wieniarska J., 2012. Effect of foliar applications of Goëmar® BM 86 and soil applied calcium nitrate on yield and berry quality of two blue honeysuckle cultivars. Acta Sci. Pol., Hortorum Cultus, 11(1), 133–144.
Thompson M.M., 2006. Introducing haskap, japanese blue honeysuckle. J. Amer. Pomol. Soc., 60(4), 164–168.
Thompson M.M., Barney D.L., 2007. Evaluation and breeding of haskap in North America. J. Amer. Pomol. Soc., 61(1), 25–33.
Truta E., Vochita G., Rosu C.M., Zamfirache M.M., Olteanu Z., Oprica L., 2013. Karyotype traits in Romanian selections of edible blue honeysuckle. Turk. J. Biol., 37, 60–68.
Verma N., Koche V., Tiwari K.L., Mishra S.K., 2009. RAPD analysis reveals genetic variation in different populations of Trichodesma indicum – a perennial medicinal herb. African J. Biotech., 8 (18), 4333–4338.


Jacek Gawroński 
University of Life Sciences in Lublin
Jerzy Hortyński 
University of Life Sciences in Lublin
Elżbieta Kaczmarska 
University of Life Sciences in Lublin
Magdalena Dyduch-Siemińska 
University of Life Sciences in Lublin
Wojciech Marecki 
University of Life Sciences in Lublin
Agata Witorożec 
University of Life Sciences in Lublin



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