MAY THE POST-CULTURE LIQUIDS OF BACTERIA INFLUENCE ON SOYBEAN [Glycine max (L.) Merrill] HEALTHINESS?
Elżbieta PatkowskaUniversity of Life Sciences in Lublin
Marzena Błażewicz-WoźniakUniversity of Life Sciences in Lublin
Soybean is one of he most valuable plants cultivated in the world. Soybean seeds and its products contain fibre, lecithin, vitamins, especially from the B group, mineral elements, isoflavones (genistein and daidzein), phytic acid, proteanase and saponin inhibitors. The object of the studies were the plants of soybean ‘Aldana’ cv. grown from the seeds dressed directly before the sowing with post-culture liquids Pseudomonas sp. Ps 255 and Bacillus sp. B 73. The studies also considered a combination with chemical seed dressing with Zaprawa Oxafun T and a combination without any dressing. The effectiveness of post-culture liquids of antagonistic bacteria was estimated in protecting soybean from soil fungi. Post-culture liquids had a positive effect on the number, health status and yielding of the studied plants. Soybean was mainly infected by Fusarium culmorum, F. oxysporum, Phoma exigua, Rhizoctonia solani and Sclerotinia sclerotiorum. Species Phomopsis sojae was additionally isolated from the infected plants. Those fungi were isolated much less frequently from the plants in combinations with post-culture liquids
of antagonistic bacteria than from reference plants. A reverse relation was observed in the case of the occurrence of saprobiotic fungi from genera Gliocladium, Penicillium and Trichoderma.
Keywords:biological control, Bacillus sp., Pseudomonas sp., phytopathogens
Akhukorala S.N.P., Fernando W.G.D., Rashid K.Y., 2009. Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry. Can. J. Microbiol. 55, 9, 1021–1032.
Castillo-Pando M.S., Nair N.G., Emmett R.W., Wicks T.J., 1997. Inhibition in pycnidial viability of Phomopsis viticola on canes in situ as an aid to reducing inoculum potential of cane and leaf blight disease of grapevines. Australas. Plant Pathol. 26, 21–25.
Chincholkar S.B., Chaudhari B.L., Rane M.R., Sarode P.D., 2007. Fungal phytopathogen suppression using siderophoregenic bioinoculants. [in:]: Biological control of plant diseases (eds. Chincholkar S. B., Mukerji K.G.). Binghampton, USA, Haworth Food & Agricultural Products Press, 401–417.
De R.K., Dwivedi R.P., Narain U., 2003. Biological control of lentil wilt caused by Fusarium oxysporum f. sp. lentis. Ann. Plant Prot. Sci. 11, 1, 46–52.
Dehestani A., Kazemitabar K., Ahmadian G., Jelodar N.B., Salmanian A.H., Seyedi M., Rahimian H., Ghasemi S., 2010. Chitinolytic and antifungal activity of a Bacillus pumilis chitinase expressed in Arabidopsis. Biotech. Letters 32, 4, 539–546.
Dipali W., Ingle R.W., Deshmukh R.W., 2007. Screening of vegetable market wastes fungi for their cellulolytic activities in vitro. J. Plant Dis. Sci. 2, 1, 91–93.
Domenech J., Reddy M.S., Kloepper J.W., Ramos B., Gutierrez-Mañero J., 2006. Combined application of the biological produkt LS213 with Bacillus, Pseudomonas or Chryseobacterium from growth promotion and biological control of soil-borne diseases in pepper and tomato. Biolog. Control 51, 2, 245–258.
Farhan H.N., Hameed A.T., Aobad H.M., 2010. The biological activity of some Pseudomonas sp. isolates on growth of three plant pathogenic fungi under incubator conditions. Advances in Environ. Biol. 4, 1, 53–57.
Khilari K., Mukhopadhyay A.N., Yadav R. S., 2008. Effect of volatile and non-volatile compounds of T. koningii and G. virens on growth S. rolfsii, R. solani and F. oxysporum f. sp. ciceri. Environ. Ecol. 26, 4B, 1961–1964.
Kućmierz J., Nawrocki J., Klata E., Stadnik U., 2010. Skuteczność kilku preparatów biotechnicznych w zwalczaniu Rhizoctonia solani Kühn i Sclerotinia sclerotiorum (Lib.) de Bary. Zesz. Prob. Postęp. Nauk Rol. 554, 71–76.
Kurzawińska H., Mazur S., 2009. The evaluation of Pythium oligandrum and chitosan in control of Phytophthora infestans (Mont.) de Bary on potato plants. Folia Hortic. 21/2, 13–23.
Oktaba W., 1987. Metody statystyki matematycznej w doświadczalnictwie. PWN, Warszawa.
Orlikowski L.B., Skrzypczak Cz., 2003. Biocides in the control of soil-borne and leaf pathogens. Hortic. Veget. Grow. 22, 426–433.
Patkowska E., 2009a. Effect of bio-products on bean yield and bacterial and fungal communities in the rhizosphere and non-rhizosphere. Polish J. Environ. Stud. 18, 2, 255–263.
Patkowska E., 2009b. Effect of chitosan and Zaprawa Oxafun T on the healthiness and communities of rhizosphere microorganisms of runner bean (Phaseolus coccineus L.). Ecolog. Chem. Eng. S, 16, (S2): 163–174.
Patkowska E., 2010. Use of chemical dressing and post-culture liquids of antagonistic bacteria in the protection of runner bean (Phaseolus coccineus L.). Ecolog. Chem. Eng. A, 17(9), 1153–1160.
Patkowska E., Konopiński M., 2011. Cover crops and soil-borne fungi dangerous towards the cultivation of salsify (Tragopogon porrifolius var. sativus (Gaterau) Br.). Acta Sci. Pol., Hortorum Cultus 10 (2), 167–181.
Patkowska E., Pięta D., 2010. Use of chemical dressing and post-culture liquids of antagonistic fungi in the protection of runner bean (Phaseolus coccineus L.) from soil-borne fungi. Ecolog. Chem. Eng. A, 17(9), 1161–1169.
Pedersen A.L., Ekelund F., Johansen A., Winding A., 2010. Interaction of bacteria-feeding soil flagellates and Pseudomonas spp. Biol. and Fertility of Soils 46, 2, 151–158.
Pięta D., Patkowska E., 2003. The role of antagonistic fungi and bacteria limiting the occurrence of some phytopathogens inhabiting the soybean soil environment. EJPAU, Horticulture, 6, 2. http://www.ejpau.media.pl
Rajeswardi B., Kumari K.V.S.M., 2009. Bioagents and fungicides for the management of seed and seedling diseases of soybean. Indian J. Plant Protec. 37, ½, 127–131.
Roberti R., Zakrisson E., Flaming F., de Vero L., 2002. Antagonistic fungi producing hydrolytic enzymes, active in degrading the cell wall of some root rot pathogens (Fusarium spp.) of wheat. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 109, 101–108.
Sandikar B.M., Awasthi R.S., 2009/2010. Preparation and shelf-live study of Pseudomonas and Bacillus bioformulations against phytopathogenic Pythium and Fusarium species. Int. J. Plant Prot. 2, 2, 251–254.
Saniewska A., Orlikowski L. B., Sobiczewski P., 1995. Effectiveness of Bacillus sp. in the control of Phytophthora cryptogea Pethybr. et Laff. M. Mańka (ed.), Environmental biotic factors in integrated plant disease control, Polish Phytopathol. Soc., Poznań, 479–184.
Selin C., Habibian R., Poritsanos N., Athukorala S.N.P., Fernando D., Kievit T.R.de, 2010. Phenazines are not essential for Pseudomonas chlororaphis PA23 biocontrol of Sclerotinia sclerotiorum, but do play a role in biofilm formation. FEMS Microbiol. Ecol. 71, 1, 73–83.
Shovan L.R., Bhuiyan M.K.A., Begum J.A., Pervez Z., 2008. In vitro control of Colletotrichum dematium causing anthracnose of soybean by fungicides, plant extracts and Trichoderma harzianum. Int. J. Sustain. Crop Prod. 3, 3, 10–17.
Srivastava R., Shalini R., 2008. Antifungal activity of Pseudomonas fluorescens against different plant pathogenic fungi. EJEFChem. 7, 4, 2789–2796.
Thasana N., Prapagdee B., Rangkadilok N., Sallabhan R., Aye S.L., Ruchirawat S., Loprasert S., 2010. Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated β-amino acid. FEBS Letters 584, 14, 3209–3214.
Yedidia I., Benhamou N., Kapulnik Y., Chet I., 2000. Induction and accumulation of PR proteins activity during stages of root colonization by the mycoparasite Trichoderma harzianum strain T-203. Plant Physiol. Biochem. 38, 863–873.
Ye Li-min, Xu Fen-fen, Xiong Zhen-huan, Wu Gao-yan, 2009. Effect of chitosan treatment on the yield, quality and diseases of soybean. Hubei Agricul. Sci., China, 07,
Zhang J.X., Xue A.G., 2010. Biocontrol of sclerotinia stem rot (Sclerotinia sclerotiorum) of soybean using novel Bacillus subtilis strain SB24 under control conditions. Plant Pathol. 59, 2, 382–391.
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.