Skip to main navigation menu Skip to main content Skip to site footer

Vol. 25 No. 5 (2026):

Review paper

Occurrence and properties of endophytic fungi of thyme (Thymus spp.)

DOI: https://doi.org/10.24326/asphc.2026.5742
Submitted: 11 July 2026
Published: 30.10.2026

Abstract

Endophytic fungi constitute an important yet still poorly explored component of the microbiome of thyme (Thymus spp.). This review summarizes the current knowledge on their diversity, ecological functions, and biotechnological potential based on literature published up to April 2026. Reported endophytes represent a diverse taxonomic community, comprising 49 genera and 53 species most of which belong to common ascomycete genera, such as Alternaria, Fusarium, Colletotrichum, Aspergillus, Penicillium, Phoma, and Trichoderma. Current evidence from experiments carried out in planta indicates that these microorganisms may contribute to plant growth promotion, enhanced tolerance to abiotic stress, modulation of essential oil production, and protection against plant pathogens through the synthesis of bioactive metabolites and volatile organic compounds. Moreover, in in vitro assays several isolates have demonstrated antimicrobial, antioxidant, cytotoxic, and enzyme-producing activities, highlighting their promising applications in sustainable agriculture and biotechnology. Although significant progress has been achieved, many aspects of the ecology, host specificity, and functional interactions of thyme-associated fungal endophytes remain insufficiently understood.

References

  1. Abdelhamid, S.A., Abo Elsoud, M.M., El-Baz, A.F. et al. (2024). Optimisation of indole acetic acid production by Neopestalotiopsis aotearoa endophyte isolated from Thymus vulgaris and its impact on seed germination of Ocimum basilicum. BMC Biotechnol. 24(1), 46. https://doi.org/10.1186/s12896-024-00872-3
  2. Abdel-Hamid, M.S., Fouda, A., El-Ela, H.K.A. et al. (2021). Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents. Biomol. Concepts 12(1), 175–196. https://doi.org/10.1515/bmc-2021-0019
  3. Adeleke, B.S., Fadiji, A.E., Ayilara, M.S. et al. (2022). Strategies to enhance the use of endophytes as bioinoculants in agriculture. Horticulturae 8(6), 498. https://doi.org/10.3390/horticulturae8060498
  4. Agrawal, S., Bhatt, A. (2023). Microbial endophytes: emerging trends and biotechnological applications. Curr. Microbiol. 80(8), 249. https://doi.org/10.1007/s00284-023-03349-2
  5. Ahmed, A.M., Mahmoud, B.K., Millán-Aguiñaga, N. et al. (2023). The endophytic Fusarium strains: a treasure trove of natural products. RSC Advan. 13(2), 1339–1369. https://doi.org/10.1039/d2ra04126j
  6. Ali, E.A.M., Aziz Ahmed, A.A., El-Ela Sayed, M.A. et al. (2018). Antioxidant activity and cytotoxicity of extracts of Thymus vulgaris L. and their associated endophytic fungi. Egypt. J. Experim. Biol. 14(1), 107–116. https://doi.org/10.5455/egy-jebb.20180122025355
  7. Bahadori, S., Attar, F., Osaloo, S.K. et al. (2026). Biogeographical and taxonomic insights into Iranian Thymus (Lamiaceae): Thymus coriifolius, and T. jankae and T. syriacus. Phytotaxa 744(2), 103–122. https://doi.org/10.11646/phytotaxa.744.2.1
  8. Bartolucci, F., Nachychko, V. (2025). The Euro+Med treatment of Thymus (Lamiaceae): nomenclature, typification of names and distribution updates. Willdenowia 55(1), 295–304. https://doi.org/10.3372/wi.55.14
  9. Bartolucci, F., Peruzzi, L., Passalacqua, N. (2013). Typification of names and taxonomic notes within the genus Thymus L. (Lamiaceae). Taxon 62(6), 1308–1314. https://doi.org/10.12705/626.1
  10. Celep, F., Dirmenci, T. (2017). Systematic and biogeographic overview of Lamiaceae in Turkey. Nat. Volat. Essent. Oils 4(4), 14–27. https://izlik.org/JA62MN24YT
  11. Checcucci, A., Maida, I., Bacci, G. et al. (2017). Is the plant-associated microbiota of Thymus spp. adapted to plant essential oil? Res. Microbiol. 168(3), 276–282. https://doi.org/10.1016/j.resmic.2016.11.004
  12. Crous, P.W., Sandoval-Denis, M., Costa, M.M. et al. (2022). Fusarium and allied fusarioid taxa (FUSA). 1. Fungal Syst. Evol. 9(1), 161–200. https://doi.org/10.3114/fuse.2022.09.08
  13. Delbari, Y., Mohassel, Y., Kakaei, E. et al. (2023). Identification and anti-bacterial property of endophytic actinobacteria from Thymes kotschyanus, Allium hooshidaryae, and Cerasus microcarpa. Sci. Rep. 13(1), 13145. https://doi.org/10.1038/s41598- 023-40478-x
  14. Del Valle, J.C., Martín-Carretié, E.M., García-Cárdenas, F.J. et al. (2026). Ecological and genetic determinants of essential oil diversity in Mediterranean Thymus. Biochem. Syst. Ecol. 127, 105260. https://doi.org/10.1016/j.bse.2026.105260
  15. Dolatabadi, H.K., Goltapeh, E.M., Moieni, A. et al. (2011). Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments. Symbiosis 53(1), 29–35. https://doi.org/10.1007/ s13199-010-0104-0
  16. Drew, B.T., Sytsma, K.J. (2012). Phylogenetics, biogeography, and staminal evolution in the tribe Mentheae (Lamiaceae). Am. J. Bot. 99(5), 933–953. https://doi.org/10.3732/ajb.1100549
  17. El-Ela Sayed, M.A., Ali E.A.M., Aziz Ahmed, A.A. et al. (2020). Antimicrobial activity of Thymus vulgaris L. and associated endophytes extracts. Egypt. J. Experim. Biol. 16(2), 141. https://doi.org/10.5455/egyjebb.20200709014142
  18. Etri, K., Pluhár, Z. (2024). Exploring chemical variability in the essential oils of the Thymus genus. Plants 13(10), 1375. https://doi. org/10.3390/plants13101375
  19. Federici, S., Galimberti, A., Bartolucci, F. et al. (2013). DNA barcoding to analyse taxonomically complex groups in plants: the case of Thymus (Lamiaceae). Bot. J. Linnean Soc. 171(4), 687–699. https://doi.org/10.1111/boj.12034
  20. Fisher, P.J., Petrini, O., Amezquita, M.M. (1992). Endophytic fungi from Alpine and Mediterranean species of Thymus. Nova Hed¬wigia 55(3–4), 473–477.
  21. García‐Cárdenas, F.J., Ortiz, M.Á., del Valle, J.C. et al. (2026). Patterns of interploidy admixture in polyploid complexes: insights from Thymus sect. Mastichina (Lamiaceae). Mol. Ecol. 35(3), e70259. https://doi.org/10.1111/mec.70259
  22. Gupta, A., Meshram, V., Gupta, M. et al. (2023). Fungal endophytes: Microfactories of novel bioactive compounds with therapeutic interventions. A comprehensive review on the biotechnological developments in the field of fungal endophytic biology over the last decade. Biomolecules 13(7), 1038. https://doi.org/10.3390/biom13071038
  23. Halat, D.H., Krayem, M., Khaled, S. et al. (2022). A focused insight into thyme: Biological, chemical, and therapeutic properties of an indigenous Mediterranean herb. Nutrients 14(10), 2104. https://doi.org/10.3390/nu14102104
  24. Kamyab, A., Samsampour, D., Ahmadinasab, N., Bagheri, A. (2024). Lamiaceae family-derived endophytic fungi: induced tolerance to drought stress in Thymus vulgaris plants. BMC Plant Biol. 24(1), 1104. https://doi.org/10.1186/s12870-024-05764-4
  25. Li, J.F., Jiang, H.B., Jeewon, R. et al. (2022). Alternaria: Update on species limits, evolution, multi-locus phylogeny, and classification. Stud. Fungi 8(1), 1–61. http://doi.org/sif-0024-0001
  26. López, P., Pastrana, A.M., Marin, M.V. et al. (2026). Molecular characterization reveals three Neopestalotiopsis species causing strawberry disease outbreaks in Spain. Front. Plant Sci. 17, 1830265. https://doi.org/10.3389/fpls.2026.1830265
  27. Ma, L., Qu, H., Guo, Z. et al. (2023). Identification and antifungal activity of endophytic fungus Trichoderma samuelsii 2-63 strain from Thymus mongolicus Ronn. Chin. J. Pest. Sci. 25(2), 377–387. https://doi.org/10.16801/j.issn.1008-7303.2023.0012
  28. Machowicz-Stefaniak, Z., Zimowska, B., Zalewska, E. (2002). Fungi colonizing various organs of thyme (Thymus vulgaris L.) cultivated in the region of Lublin. Acta Agrobot. 55(1), 185–197.
  29. Machowicz-Stefaniak, Z., Zimowska, B., Zalewska, E. (2008). The occurrence and pathogenicity of Phoma exigua Desm. var. exigua for selected species of herbs. Acta Agrobot. 61(2), 157–166. https://doi.org/10.5586/aa.2008.044
  30. Malagaris, P. (2011). Biology and ecology of Asphondylia coridothymi (Diptera: Cecidomyiidae) inducing galls on Coridothymus capitatus on the island of Samos, Greece. Acta Soc. Zool. Bohem. 75, 239–251.
  31. Manathunga, K.K., Gunasekara, N.W., Meegahakumbura, M.K. et al. (2024). Exploring endophytic fungi as natural antagonists against fungal pathogens of food crops. J. Fungi 10(9), 606. https://doi.org/10.3390/jof10090606
  32. Masumi, S., Mirzaei, S., Kalvandi, R. et al. (2014). Asparaginase and amylase activity of thyme endophytic fungi. J. Crop Prot. 3(5), 655–662. https://doi.org/10.48311/jcp.2014.1176
  33. Masumi, S., Mirzaei, S., Zafari, D. et al. (2015a). Isolation, identification and endophytic bacteria and yeasts isolated from Thymus. Progr. Biol. Sci. 5(1), 33–42.
  34. Mirzaei, S., Masumi, S. (2023). The antimicrobial activity of endophytic fungi isolated from Thymus spp. J. Med. Plants By-prod. 1, 117–124. https://doi.org/10.22092/jmpb.2022.342405.1192
  35. Mohamad, O.A.A., Ma, J.B., Liu, Y.H. et al. (2020). Beneficial endophytic bacterial populations associated with medicinal plant Thymus vulgaris alleviate salt stress and confer resistance to Fusarium oxysporum. Front. Plant Sci. 11, 47. https://doi.org/10.3389/fpls.2020.00047
  36. Mohamad, O.A.A., Liu, Y.H., Huang, Y. et al. (2022). The metabolic potential of endophytic actinobacteria associated with medicinal plant Thymus roseus as a plant-growth stimulator. Microorganisms 10(9), 1802. https://doi.org/10.3390/microorganisms10091802
  37. Morales, R. (2002). The history, botany and taxonomy of the genus Thymus. In: Thyme: The Genus Thymus, Stahl-Biskup, E., Sáez, F. (eds.). Taylor & Francis, London, 1–44.
  38. Morelli, M., Bahar, O., Papadopoulou, K.K. et al. (2020). Role of endophytes in plant health and defense against pathogens. Front. Plant Sci. 11, 1312. https://doi.org/10.3389/fpls.2020.01312
  39. Musa, Z., Ma, J., Egamberdieva, D. et al. (2020). Diversity and antimicrobial potential of cultivable endophytic actinobacteria asso¬ciated with the medicinal plant Thymus roseus. Front. Microbiol. 11, 191. https://doi.org/10.3389/fmicb.2020.00191
  40. Najar, B., Pistelli, L., Ferri, B. et al. (2021). Crop yield and essential oil composition of two Thymus vulgaris chemotypes along three years of organic cultivation in a hilly area of central Italy. Molecules 26(16), 5109. https://doi.org/10.3390/molecules26165109
  41. Nicoletti, R., Di Vaio, C., Cirillo, C. (2020). Endophytic fungi of olive tree. Microorganisms 8(9), 1321. https://doi.org/10.3390/microorganisms8091321
  42. Nicoletti, R., Fiorentino, A. (2015). Plant bioactive metabolites and drugs produced by endophytic fungi of Spermatophyta. Agriculture 5(4), 918–970. https://doi.org/10.3390/agriculture5040918
  43. Nicoletti, R., Zimowska, B. (2023). Endophytic fungi of hazelnut (Corylus avellana). Plant Prot. Sci. 59(2), 107–123. https://doi.org/10.17221/133/2022-PPS
  44. Nieto, G. (2020). A review on applications and uses of Thymus in the food industry. Plants 9(8), 961. https://doi.org/10.3390/plants9080961
  45. Nieto-Lugilde, D., Del Valle, J.C., Martín-Carretié, E.M. et al. (2026). Genomic structure, introgression, niche overlap, and morphological variation challenges species delimitation in Thymus sect. Mastichina. Ann. Bot. 138(4), 1194–1209. https://doi.org/10.1093/aob/mcag151
  46. Olama, S.M., Sheata, R., El Shiekh, H. et al. (2023). Phytochemistry and biodiversity of endophytic fungal metabolites isolated from medicinal plants. Al-Azhar J. Agric. Res. 48(2), 42–58. https://doi.org/10.21608/ajar.2024.224023.1198
  47. Pandey, S.S., Jain, R., Bhardwaj, P. et al. (2022). Plant probiotics–endophytes pivotal to plant health. Microbiol. Res. 263, 127148. https://doi.org/10.1016/j.micres.2022.127148
  48. Rabiey, M., Hailey, L.E., Roy, S.R. et al. (2019). Endophytes vs tree pathogens and pests: can they be used as biological control agents to improve tree health? Eur. J. Plant Pathol. 155(3), 711–729. https://doi.org/10.1007/s10658-019-01814-y
  49. Ray, P., Craven, K.D. (2016). Sebacina vermifera: a unique root symbiont with vast agronomic potential. World J. Microbiol. Bio¬technol. 32(1), 16. https://doi.org/10.1007/s11274-015-1970-7
  50. Saleem, S., Sekara, A., Pokluda, R. (2022). Serendipita indica – A review from agricultural point of view. Plants 11(24), 3417. https://doi.org/10.3390/plants11243417
  51. Soorni, A., Borna, T., Alemardan, A. et al. (2019). Transcriptome landscape variation in the genus Thymus. Genes 10(8), 620. https://doi.org/10.3390/genes10080620
  52. Waheed, M., Hussain, M.B., Saeed, F. et al. (2024). Phytochemical profiling and therapeutic potential of thyme (Thymus spp.): A medicinal herb. Food Sci. Nutri. 12(12), 9893–9912. https://doi.org/10.1002/fsn3.4563
  53. Yadav, A.N., Kour, D., Kaur, T. et al. (2022). Endophytic fungal communities and their biotechnological implications for agro-envi¬ronmental sustainability. Folia Microbiol. 67(2), 203–232. https://doi.org/10.1007/s12223-021-00939-0
  54. Zerroug, A., Sadrati, N. (2022). Endophytic fungi isolated from Thymus algeriensis with good antimicrobial activities. Turk. J. Agric. Food Sci. Technol. 10(10), 1789–1798. https://doi.org/10.24925/turjaf.v10i10.1789-1798.5395
  55. Zhao, M., Su, H., Huang, Y. et al. (2023). Plant growth-promoting properties and anti-fungal activity of endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta in arid lands. J. Arid Land 15(11), 1405–1420. https://doi.org/10.1007/s40333-023-0071-y
  56. Zimowska, B. (2022). Phoma on medicinal and aromatic plants. In: Phoma: Diversity, taxonomy, bioactivities, and nanotechnology. Springer International Publishing, Cham, 37–55.
  57. Zimowska, B., Becchimanzi, A., Krol, E.D. et al. (2021). New Cladosporium species from normal and galled flowers of Lamiaceae. Pathogens 10(3), 369. https://doi.org/10.3390/pathogens10030369
  58. Zimowska, B., Okoń, S., Becchimanzi, A. et al. (2020). Phylogenetic characterization of Botryosphaeria strains associated with Asphondylia galls on species of Lamiaceae. Diversity 12(2), 41. https://doi.org/10.3390/d12020041
  59. Zimowska, B., Viggiani, G., Nicoletti, R. et al. (2017). First report of the gall midge Asphondylia serpylli on thyme (Thymus vulgaris), and identification of the associated fungal symbiont. Ann. Appl. Biol. 171(1), 89–94. https://doi.org/10.1111/aab.12360
  60. Zubek, S., Błaszkowski, J. (2009). Medicinal plants as hosts of arbuscular mycorrhizal fungi and dark septate endophytes. Phyto¬chem. Rev. 8(3), 571–580. https://doi.org/10.1007/s11101-009-9135-7

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.