Micropropagation of stevia (Stevia rebaudiana Bert.) through node explants isolated from in vitro produced plants
ROMUALD DOLIŃSKI
Instytut Genetyki, Hodowli i Biotechnologii Roślin Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 LublinEWELINA JABŁOŃSKA
Instytut Genetyki, Hodowli i Biotechnologii Roślin Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 LublinAbstract
The main aim of the study was to assess the capacity of Stevia rebaudiana population for micropropagation through node explants. Primary explants were shoots apex collected from plants growing in glasshouse. Node explants were excised from sterile plants developed in vitro. They were placed on MS medium supplemented with different BAP concentrations (0.5–4 mg.dm-3). After 3 weeks, explants were transferred onto the fresh medium with the same BAP concentrations and 0.1 mg.dm-3 GA3. Within another 3 weeks each node explants regenerated shoots. The maximum shoots formation (11.36 shoots per explant) was observed on the medium containing 1.0 mg.dm-3 BAP and 0.1 mg.dm-3 GA3. Each of the BAP and GA3 combinations produced much callus. The maximum callus proliferation was found on MS medium containing 1.0 mg.dm-3 BAP. In vitro regenerated shoots were rooted in MS medium supplemented with various concentrations of IBA (0.25–1.5 mg.dm-3). Within 5 weeks each shot produced roots. The maximum root number (10.04) was obtained at 1.0 mg.dm-3 IBA. Forty seedlings were moved into the pots filled with sterile soil mixture and subjected to the hardening process. 92.5% of the plants survived acclimatization.
Keywords:
Stevia rebaudiana, micropropagation, node explantsReferences
Ahmed M.B., Salahin M., Karim R., Razvy M. A., Hannan M. M., Sultana R., Hossain M., Islam R., 2007. An efficient method for in vitro clonal propagation of a newly introduced plant Stevia rebaudiana Bertoni) in Bangladesh. Am-Euras. J. Sci. Res. 2(2), 121–125.
Anbazhagan M., Kalpana M., Rajendran R., Natarajan V., Dhanavel D., 2010. In vitro production of Stevia rebaudiana Bertoni. Emir. J. Food Agric. 22(3), 216–222.
Bach A., Pawłowska B., 2009. Procesy rozwojowe w kulturze in vitro i typy kultury. W: S. Malepszy (red.), Biotechnologia roślin. PWN, Warszawa, 21–40.
Benford D.J., DiNovi M., Schlatter J., 2006. Safety evaluation of certain food additives: Stevio glicosides.. WHO Food Addit. Ser. 5, 117–144.
Brandle J.E., Starratt A.N., Gijzen M., 1998. Stevia rebaudiana: its agricultural, biological and chemical properties. Can. J. Plant Sci. 78, 527–536.
Cardello H.M.A.B., Dasilva M.A.P.A., Damasio M.H., 1999. Measurement of the relative sweetness of Stevia extract. Aspartame and Cyclamate/Saccharin compared to Sucrose at different concentrations. Plant Foods Hum. Nutr. 54, 119–130.
Carneiro J.W.P., Muniz A.S., Guedes T.A., 1997. Greenhouse bedding plant production of Stevia rebaudiana (Bert) bertoni. Can. J. Plant Sci. 77, 473–474.
Das A., Gantait S., Mandal N., 2011. Micropropagation of an elite medicinal plant: Stevia rebaudiana Bert. Int. J. Agric. Res. 6(1), 40–48.
Debnath M., 2008. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. J. Med. Plant. Res. 2, 045–051.
Felippe G.M., Lucas N.M.C., Behar L., Oliveira M.A.C., 1971. Observacoes a respeito de germinaco de Stevia rebaudiana Bert. Hoehnea 1, 81–93.
Genus J.M.C., 2003. Molecules of interest, Stevioside. Phytochemistry 64, 913–921.
Huxtable R.J,. 2002. Pharmacology and toxicology of stevioside, rebaudioside A, and steviol. In: A.D. Kinghorn (ed.). Stevia. The genus Stevia. Med. Aromat. Plants Ind. Profiles 19, 161–177.
Jagatheeswari D., Ranganathan P., 2012. Studies on micropropagation of Stevia rebaudiana Bert. Int. J. Pharm.. Biol. Archiv. 3(2), 315–320.
Kennelly E.J., 2002. Sweet and non-sweet consituents of Stevia rebaudiana. Bertoni. In: A.D. Kinghorn (ed.), Stevia. The genus Stevia. Med. Aromat. Plants. Ind. Profiles 19, 68–85.
Laribi B., Rouatbi N., Kouki K., Bettaieb T., 2012. In vitro propagation of (Stevia rebaudiana Bert.). A non caloric sweetener and antidiabetic medicinal plant. Int. J. Med. Arom. Plants 2 (2), 333–339.
Larkin P.J., Scowcroft W.R., 1981. Somaclonal variation – a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60, 197–214.
Lemus-Mondaca R., Vega-Galez A., Zura-Bravo L., Ah-Hen K., 2012. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem. 132, 1121–1132.
Liu J., Li S.F.Y., 1995. Separation and determination of stevia sweeteners by capillary electroforesis and high performance liquid chromatography. J. Liq. Chromatogr. 18(9), 1703–1719.
Matsukubo T., 2006. Socrose substitutes and their role in caries prevention. Int. Dent. J. SCN 56(3), 119–130.
Megaji N.W., Kumar J.K., Singh V., Kaul V.K., Ahuja P.S., 2005. Introducing Stevia Rebaudiana a natural zero-calorie sweeteners. Curr. Cell Sci. 88(5), 801–804.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Nakamura S., Tamura Y., 1985. Variation in the main glycosides of Stevia (Stevia rebaudiana Bertoni). Jpn J. Trop. Agric. 29, 109–116).
Oktaba W., 2000. Metody statystyki matematycznej w doświadczalnictwie. Wyd. 5, Wyd. AR w Lublinie, 15–23, 35–47.
Rafiq M., Dahot M.U., Mangrio S.M., Naqvi H.A., Qarshi I.A., 2007. In vitro clonal propagation and biochemical analysis of field established Stevia rebaudiana Bertoni. Pak. J. Bot. 39(7), 2467–2474.
Sakaguchi M., Kan T., 1982. Japanese researches on Stevia rebaudiana (Bert.) and stevioside. Ci Cult. 34, 235–248.
Savita S.M., Shella K., Sunanda S., Shankar A.G., Ramakrishna P. 2004. Stevia rebaudiana – a functional component for food industry. J. Human Ecol. 15, 261–264.
Soejarto D.D., 2002. Ethnobotany of Stevia and Stevia rebaudiana. In: A.D. Kinghorn (ed.), Stevia. The genus Stevia. Med. Aromat. Plants Ind. Profiles 19, 40–67.
Starratt A.N., Kirbyb C.W., Posca R., Brandle J.E., 2002. Rebaudioside F, a diterpene glycoside from Stevia rebaudiana. Phytochemistry 59, 367.
Thiyagarajan P., Venkatachalam P., 2012. Large scale in vitro propagation of Stevia rebaudiana (bert) for commercial application: Pharmaceutically important and antidiabetic medicinal herb. Indus. Crops Prod. 37, 111–117.
Verma S., Yadav K., Singh N., 2011. Optimization of the protocols for surface sterilization, regeneration and acclimatization of Stevia rebaudiana Bertoni. Am-Euras. J. Agric. Environ. Sci. 11(2), 221–227.
Yang Y., Hsing Y., Chang W., 1981. Clonal propagation of Stevia rebaudiana Bertoni through axillary shoot proliferation in vitro. Bot. Bull. Acad. Sin. 22, 57–62.
Instytut Genetyki, Hodowli i Biotechnologii Roślin Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 Lublin
Instytut Genetyki, Hodowli i Biotechnologii Roślin Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 Lublin
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