In vitro SELECTION FOR LEAD TOLERANCE IN SHOOT CULTURE OF Daphne SPECIES
Alina Wiszniewska
University of Agriculture in KrakówEwa Hanus-Fajerska
University of Agriculture in KrakówSylwester Smoleń
University of Agriculture in KrakówEwa Muszyńska
University of Agriculture in KrakówAbstract
In vitro culture may provide a suitable environment for selection of heavy-metal tolerant plantlets. Such clones of woody plants could be valuable material applicable to soil remediation. In in vitro culture conditions shoots of Daphne jasminea Sibth. & Sm. and Daphne tangutica Maxim. (Thymelaeaceae) were grown on media supplemented with 0.1, 0.5 and 1.0 mM lead nitrate. Level of lead bioaccumulation, growth parameters, content of photosynthetic pigments, and mineral status of cultured shoots were investigated. D. jasminea has grown vigorously on Pb2+-containing media, with growth tolerance index reaching 73–89%, depending on concentration applied, and the highest growth value was obtained in the presence of 1.0 mM lead nitrate. In vitro propagation of D. tangutica shoots was slightly inhibited by lead ions, however the growth tolerance index has increased up to 152% on medium with 1.0 mM Pb(NO3)2. In both studied species the highest
content of accumulated lead, as well as the value of bioconcentration factor, were found in shoots grown on 0.1 mM lead nitrate. D. tangutica accumulated over two times as much lead in comparison with D. jasminea. Chlorophyll content in D. jasminea was not affected by applied lead nitrate doses, while in D. tangutica stimulation of chlorophyll, as well as carotenoid, synthesis occurred. In tested concentrations lead nitrate had no toxic effect on the level of shoot nutrition. Detected levels of essential and trace elements were still high enough to maintain undisturbed growth and development of cultured shoots. This is first report confirming the suitability of in vitro selection for obtaining of vigorous, proliferative, tolerant to elevated lead concentration shoots of ornamental Daphne species.
Keywords:
bioaccumulation, heavy metal, in vitro culture, mineral nutrition, ThymelaeaceaeReferences
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Stomp, A-M., Han, K.H., Wilbert, S., Gordon, M.P. (1993). Genetic improvement of tree species for remediation of hazardous wastes. In Vitro Cell Dev. Biol., 29P, 227–232.
Sytar, O., Kumar, A., Latowski, D., Kuczyńska, P., Strzałka, K., Prasad, M.N.V. (2013). Heavy metal-induced oxidative damage, defense reactions, and detoxification mechanisms in plants. Acta Physiol. Plant., 35, 985–999.
Toan, N.B., Ve, N.B., Debergh, P. (2004). Tissue culture approaches for the selection of aluminium-tolerant Citrus species in the Mekong Delta of Vietnam. J. Hortic. Sci. Biotech., 79(6), 911–916.
Trigueros, D., Mingorance, M.D., Rossini Oliva, S. (2012). Evolution of the ability of Nerium oleander L. to remediate Pb-contaminated soils. J. Geochem. Explor., 114, 126–133.
Wellburn, A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol., 144(3), 307–313.
Wierzbicka, M. (1998). Lead in the apoplast of Allium cepa L. root tips – ultrastructural studies. Plant Sci., 133,105–119.
Wierzbicka, M., Przedpełska, E., Ruzik, R., Querdane, L., Polec-Pawlak, K., Jarosz, M., Szponar, J., Szakiel, A. (2007). Comparison of the toxicity and distribution of cadmium and lead in plant cells. Protoplasma, 231, 99–111.
Wiszniewska, A., Hanus-Fajerska, E., Grabski, K., Tukaj, Z. (2013). Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae). In Vitro Cell Dev. Biol. Plant, 49(1), 51–59.
Yu, X., Zhou, P., Zhou, X., Liu, Y. (2005). Cyanide removal by Chinese vegetation. Environ. Sci. Pollut., 12(4), 221–226.
Zhera, S.S., Arshad, M., Mahmood, T., Waheed, A. (2009). Assessment of heavy metal accumulation and their translocation in plant species. Afr. J. Biotech., 12, 2802–2810.
Ali, M.P.B., Vajpayee, P., Tripathi, R.D., Rai, U.N., Singh, S.N., Singh, S.P. (2003). Phytoremediation of lead, nickel and cooper by Salix acmophylla Boiss.: role of ntioxidant enzymes and antioxidant substances. Environ. Contam. Tox., 70, 462–469.
Alkorta, I., Hernández-Allica, J., Becerril, J.M., Amezaga, I., Albizu, I., Garbisu, C. (2004). Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead and arsenic. Rev. Environ. Sci. Bio-Technol., 3, 71–90.
Antosiewicz, D.M. (1995). The relationships between constitutional and inducible Pb-tolerance and tolerance to mineral deficits in Biscutella laevigata and Silene inflata. Environ. Exp. Bot., 35,55–69.
Baz, M., Fernandez, R.T. (2002). Evaluating woody ornamentals for use in herbicide phytoremediation. J. Am. Soc. Hort. Sci., 127, 991–997.
Bibi, M., Hussein M. (2005). Effect of copper and lead on photosynthesis and plant pigments in black gram (Vigna mungo (L.) Hepper). Environ. Contam. Tox., 74, 1126–1133.
Bystrzejewska-Piotrowska, G., Jeruzalski, M., Urban, P. (2004). Uptake and distribution of cesium and its influence on the physiological processes in cotton plants (Codiaeum variegatum). Nukleonika, 49, S35–38.
Čabala, R., Slovakova, L., El Zohri, M., Frank, H. (2011). Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L. Acta Physiol. Plant., 33,1239–1248.
Demirayak, A., Kutaby, H.G., Kilic, D., Bilgin, A., Hüseinova, R. (2011). Heavy metal accumulation in some natural and exotic plants in Samsun City. Ekoloji, 20, 1–11.
Dhankher, O.P., Li, Y., Rosen, B.P., Shi, J., Salt, D., Senecoff, J.F., Sashti, N.A., Meagher, R.B. (2002). Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and gamma-glutamylcysteine synthetase expression. Nat. Biotechnol., 20, 1140–1145.
Di Lonardo, S., Capuana, M., Arnetoli, M., Gabbrielli, R., Gonnelli, C. (2011). Exploring the metal phytoremediation potential of three Populus alba L. clones using an in vitro screening. Environ. Sci. Pollut., 18,82–90.
Doran, P.M. (2009). Application of plant tissue cultures in phytoremediation research: incentives and limitations. Biotechnol. Bioeng., 103(1), 60–76.
Fernandez, R., Bertrand, A., Casares, A., Garcia, R., Gonzalez, A., Tames, R.S. (2008). Cadmium accumulation and its effect on the in vitro growth of woody fleabane and mycorrhized white birch. Environ. Pollut., 152, 522–529.
Gatti, E. (2008). Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance. Biol. Plantarum., 52(1), 146–148.
Ghnaya, A.B., Hourmant, A., Cerantola, S., Kervarec, N., Cabon, J.Y., Branchard, M., Charles, G. (2010). Influence of zinc on soluble carbohydrate and free amino acid levels in rapeseed plants regenerated in vitro in the presence of zinc. Plant. Cell Tiss. Organ Cult., 102, 191–197.
Hanus-Fajerska, E., Wiszniewska, A., Czaicki, P. (2012). Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants. Acta Agrobot., 65(1), 21–28.
Iori, V., Petrini, F., Massacci, A., Zacchini, M. (2012). Induction of metal binding compounds and antioxidative defence in callus cultures of two black poplar (P. nigra) clones with different tolerance to cadmium. Plant Cell Tiss. Organ Cult., 108, 17–26.
Kabata-Pendias, A. (2011). Trace elements in soil and plants. Fourth edition, CRC Press, Taylor and Francis Group.
Kastori, R., Plesničar, M., Sakač, Z., Panković, D., Arsenijević‐Maksimović, I. (1998). Effect of excess lead on sunflower growth and photosynthesis. J. Plant Nutr., 21, 75–85.
Liu, D., Liu, X., Chen, Z., Xu H., Ding, X. (2010). Bioaccumulation of lead and the effects of lead on catalase activity, glutathione levels, and chlorophyll content in the leaves of wheat. Comm. Soil Sci. Plan., 41,935–944.
Liu, J., Zhou, Q., Sun, T., Ma, L.Q., Wang, S. (2008). Growth responses of three ornamental plants to Cd and Cd–Pb stress and their metal accumulation characteristics. J. Hazard. Mater., 151, 261–267.
Liu, J., Zhou, Q., Wang, S. (2010). Evaluation of chemical enhancement on phytoremediation effect of Cd-contaminated soils with Calendula officinalis L. Int. J. Phytorem., 12, 503–515.
Lloyd, G., McCown, B. (1981). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb. Proc. Intl. Plant Prop. Soc., 30,421–437.
Martinez, M., Bernal, P., Almela, C., Velez, D., Garcia-Augustin, P., Serrano, R., Navarro-Avino, J. (2006). An engineered plant that accumulates higher levels of heavy metals than Thlaspi caerulescens, with yields of 100 times more biomass in mine soils. Chemosphere, 64, 478–485.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15,473–479.
Noshad, D., Miresmaili, S., Riseman, A., Ekramoddoullah, A. (2009). In vitro propagation of seven Daphne L. species. Plant Cell Tiss. Organ Cult., 96, 201–209.
Patra, M., Bhowmik, N., Bondopadhyay, B., Shrama, A. (2004). Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environ. Exp. Bot., 52, 199–223.
Poorter, H., Niklas, K.J., Reich, P.B., Oleksyn, J., Poot, P., Moomer, L. (2011). Biomass allocation to leaves, stems and roots: meta-analyses of interspecific variation and environmental control. New Phytol., 193, 30–50.
Schützendübel, A., Polle, A. (2002). Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J. Exp. Bot. 53, 1351–1365.
Sharma, P., Dubey, R.M. (2005). Lead toxicity in plants. Brazil. J. Plant Physiol., 17, 35–52.
Soudek, P., Tykva, R., Vanĕk, T. (2004). Laboratory analyses of 137Cs uptake by sunflower, reed and poplar. Chemosphere, 55, 1081–1087.
Stomp, A-M., Han, K.H., Wilbert, S., Gordon, M.P. (1993). Genetic improvement of tree species for remediation of hazardous wastes. In Vitro Cell Dev. Biol., 29P, 227–232.
Sytar, O., Kumar, A., Latowski, D., Kuczyńska, P., Strzałka, K., Prasad, M.N.V. (2013). Heavy metal-induced oxidative damage, defense reactions, and detoxification mechanisms in plants. Acta Physiol. Plant., 35, 985–999.
Toan, N.B., Ve, N.B., Debergh, P. (2004). Tissue culture approaches for the selection of aluminium-tolerant Citrus species in the Mekong Delta of Vietnam. J. Hortic. Sci. Biotech., 79(6), 911–916.
Trigueros, D., Mingorance, M.D., Rossini Oliva, S. (2012). Evolution of the ability of Nerium oleander L. to remediate Pb-contaminated soils. J. Geochem. Explor., 114, 126–133.
Wellburn, A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol., 144(3), 307–313.
Wierzbicka, M. (1998). Lead in the apoplast of Allium cepa L. root tips – ultrastructural studies. Plant Sci., 133,105–119.
Wierzbicka, M., Przedpełska, E., Ruzik, R., Querdane, L., Polec-Pawlak, K., Jarosz, M., Szponar, J., Szakiel, A. (2007). Comparison of the toxicity and distribution of cadmium and lead in plant cells. Protoplasma, 231, 99–111.
Wiszniewska, A., Hanus-Fajerska, E., Grabski, K., Tukaj, Z. (2013). Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae). In Vitro Cell Dev. Biol. Plant, 49(1), 51–59.
Yu, X., Zhou, P., Zhou, X., Liu, Y. (2005). Cyanide removal by Chinese vegetation. Environ. Sci. Pollut., 12(4), 221–226.
Zhera, S.S., Arshad, M., Mahmood, T., Waheed, A. (2009). Assessment of heavy metal accumulation and their translocation in plant species. Afr. J. Biotech., 12, 2802–2810.
Alina Wiszniewska
University of Agriculture in Kraków
University of Agriculture in Kraków
Ewa Hanus-Fajerska
University of Agriculture in Kraków
University of Agriculture in Kraków
Sylwester Smoleń
University of Agriculture in Kraków
University of Agriculture in Kraków
Ewa Muszyńska
University of Agriculture in Kraków
University of Agriculture in Kraków
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