MOLECULAR VARIABILITY OF THE COAT PROTEIN GENE OF Prunus necrotic ringspot virus ISOLATES
Kinga Sala-Rejczak
Warsaw University of Life SciencesElżbieta Paduch-Cichal
Warsaw University of Life SciencesAbstract
We have obtained and described the nucleotide sequences corresponding to ilarvirus Prunus necrotic ringspot virus (PNRSV] coat protein (CP) gene in eleven Polish
isolates, from different Prunus and Rosa species and four isolates provided in plant material from Australia, Hungary and Italy. Virus identification was possible using specific primers allowed amplifying the 700 bp amplicon of coat protein gene. The product was obtained used IC-RT-PCR. The results indicated no association between the host species or the geographic origin and the PNRSV CP sequence specificity. The CP gene nucleotide sequence of studied isolates allowed for clustering them into the previously reported PV32-I, PV96-II and PE5-III phylogroups.
Keywords:
PNRSV, CP, IC-RT-PCR, sequencingReferences
Aparicio F., Myrta A., Terlizzi B., Pallás V., 1999. Molecular variability among isolates of Prunus necrotic ringspot virus from different Prunus spp. Phytopathology 89, 991–999.
Aparicio F., Vilar M., Perez-Paya E., Pallás V., 2003. The coat protein of Prunus necrotic ringspot virus specifically binds to and regulates the conformation of its genomic RNA. Virology 313, 213–223.
Clark M.F., Adams A.N., 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34, 475–483.
Codoñer F.M., Fares M.A., Elena, S.F., 2006. Adaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus. J. Virol. 80, 5833–5840.
Fiore N., Fajardo T.V.M., Prodan S., Herrranz M.C., Aparicio F., Montealegre J., Elena, S.F., Pallas V., Sanchez-Navarro J., 2008. Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus. Arch. Virol. 153, 909–919.
Glasa M., Betinová M., Kùdela O., Šubr Z., 2002. Biological and molecular characterisation of Prunus necrotic ringspot virus isolates and possible approaches to their phylogenetic typing. Ann. Appl. Biol. 140, 279–283.
Guo D., Maiss E., Günter A., Casper R., 1995. Prunus necrotic ringspot ilarvirus: nucleotide sequence of RNA3 and relationship to other ilarviruses based on coat protein comparision. J. Gen. Virol. 76, 1073–1079.
Hammond R.W., 2003. Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features. Arch. Virol., 148, 1195–1210.
Hammond R.W., Crosslin, J.M., 1995. The complete nucleotide sequence of RNA3 of a peach isolate of Prunus necrotic ringspot virus. Virology 208, 349–353.
Hammond R.W., Crosslin J.M., 1998. Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates. J. Gen. Virol. 79, 1815–1823.
Malinowski T., 2005. Potential problems with the reability of PCR based diagnostic methods related to plant viruses sequence variation. Phytopathpl. Pol. 35, 125–139.
Malinowski T., Komorowska B., 1998. Metoda powielania genu białka płaszcza (CP) wirusa nekrotycznej plamistości pierścieniowej drzew pestkowych (PNRSV) i jej zastosowanie. Polish Conference of Fruit Plants Protection, Skierniewice, Poland, 203 pp.
Moury B., Cardin L., Onesto J.P., Candresse T., Poupet, A., 2000. Enzyme-linked immunosorbent assay testing of shoots grown in vitro and the use of immunocapture- reverse transcriptionpolymerase chain reaction improve the detection of prunus necrotic ringspot virus in rose. Phytopathology 90, 522–528.
Moury B., Cardin L., Onesto J.P., Candresse T., Poupet, A., 2001. Survey of Prunus necrotic ringspot virus in Rose and its variability in Rose and Prunus spp. Phytopathology 91, 84–91.
Neeleman L., Van der Kuyl A.C., Bol J., 1991. Role of alfalfa mosaic virus coat protein gene in symptom formation. Virology 181, 687–693.
Nolasco G., de Blas C., Torres V., Ponz F., 1993. A method combining immunocapture and PCR amplification in a microtiter plate for the routine diagnosis of plant viruses and subviral pathogens. J. Virol. Meth. 45, 201–218.
Oliver J.E., Freer J., Andersen R.L., Cox K.D., Robinson T.L., Fuchs M., 2009. Genetic diversity of Prunus necrotic ringspot virus isolates within a cherry orchard in New York. Plant Disease 93, 599–606.
Paduch-Cichal E., 2003. First report of occurrence of viruses on some field-grown rose cultivars in Warsaw. Phytopathol. Pol. 28, 53–62.
Paduch-Cichal E., Sala-Rejczak K., Lewko J., 2007. The reaction of Prunus avium, clone F12/1 plants inoculated with PNRSV isolates obtained from different species of Prunus and rose plants. Phytopathol. Pol. 46, 13–23.
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Virology Division International Union of Microbiological Societies, Elsevier Academic Press Amsterdam, Boston, Heidelberg, London, New York, Oxford, Paris, San Diego, San Fransisco, Singapore, Sydney, Tokyo, 1049–1058.
Salem N., Mansour A., Al-Musa A., Al-Nsour A., Hammond, R., 2004. Identification and partial characterization of Prunus necrotic ringspot virus on stone fruits in Jordan. J. Plant Pathol. 86, 85–90.
Sanchez-Navarro J.A., Pallas V., 1997. Evolutionary relationships in the ilarviruses: nucleotide sequence of prunus necrotic ringspot virus RNA3. Arch. Virol. 142, 749–763.
Scott S.W., Zimmerman M.T., Ge Xin, MacKenzie D.J., 1998. The coat proteins and putative movement proteins of isolates of Prunus necrotic ringspot virus from different host species and geographic origins are extensively conserved. Europ. J. Plant Pathol. 104, 155–161.
Shintaku M., Zang L., Palukaitis P., 1992. A single amino acid substitution in the coat protein of cucumber mosaic virus induces chlorosis in tobacco. Plant Cell 4, 751–757.
Spiegel S., Holland D., Tam Y., Bar-Yaakov I., Maslein L., Rosner A., 2004. Prunus necrotis ringspot virus isolates in stone fruit germplasm accessions and cultivars in Israel. Ann. Appl. Biol. 144, 229–236.
Spiegel S., Tam Y., Maslein L., Kolber M., Nèmeth M., Rosner A., 1999. Typing Prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products. Ann. Appl. Biol. 135, 395–400.
Thompson J.D., Higgins D.G., Gibson T.J., 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673–4680.
Uemoto J.K., Scott S.W., 1992. Important disease of Prunus caused by viruses and other grafttransmissible pathogens in California and South Carolina. Plant Disease 76, 5–11
Ulubas C., Ertunc F., 2004. RPCR detection and molecular characterizatuion of Prunus necrotic ringspot virus isolaytes occurring in Turkey. J. Phytopathol. 152, 498–502.
Vašková D., Petrzik K., Karešová R., 2000. Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus. Arch. Virol. 145, 699–709.
Wetzel T., Candresse T., Macquire G., Ravelonandro M., Dunez J., 1992. A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. J. Virol. Meth. 39, (1–2), 27–37.
Yurtmen M., Spiegel S., Mastlenin M., Yilmaz M.A., Rosner A., 2002. Assessing the applicability of transcript conformtion polymorphism for differentiation among Prunus necrotic ringspot virus isolates. Phytopahol. Mediterr. 41, 92–98.
Aparicio F., Vilar M., Perez-Paya E., Pallás V., 2003. The coat protein of Prunus necrotic ringspot virus specifically binds to and regulates the conformation of its genomic RNA. Virology 313, 213–223.
Clark M.F., Adams A.N., 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34, 475–483.
Codoñer F.M., Fares M.A., Elena, S.F., 2006. Adaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus. J. Virol. 80, 5833–5840.
Fiore N., Fajardo T.V.M., Prodan S., Herrranz M.C., Aparicio F., Montealegre J., Elena, S.F., Pallas V., Sanchez-Navarro J., 2008. Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus. Arch. Virol. 153, 909–919.
Glasa M., Betinová M., Kùdela O., Šubr Z., 2002. Biological and molecular characterisation of Prunus necrotic ringspot virus isolates and possible approaches to their phylogenetic typing. Ann. Appl. Biol. 140, 279–283.
Guo D., Maiss E., Günter A., Casper R., 1995. Prunus necrotic ringspot ilarvirus: nucleotide sequence of RNA3 and relationship to other ilarviruses based on coat protein comparision. J. Gen. Virol. 76, 1073–1079.
Hammond R.W., 2003. Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features. Arch. Virol., 148, 1195–1210.
Hammond R.W., Crosslin, J.M., 1995. The complete nucleotide sequence of RNA3 of a peach isolate of Prunus necrotic ringspot virus. Virology 208, 349–353.
Hammond R.W., Crosslin J.M., 1998. Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates. J. Gen. Virol. 79, 1815–1823.
Malinowski T., 2005. Potential problems with the reability of PCR based diagnostic methods related to plant viruses sequence variation. Phytopathpl. Pol. 35, 125–139.
Malinowski T., Komorowska B., 1998. Metoda powielania genu białka płaszcza (CP) wirusa nekrotycznej plamistości pierścieniowej drzew pestkowych (PNRSV) i jej zastosowanie. Polish Conference of Fruit Plants Protection, Skierniewice, Poland, 203 pp.
Moury B., Cardin L., Onesto J.P., Candresse T., Poupet, A., 2000. Enzyme-linked immunosorbent assay testing of shoots grown in vitro and the use of immunocapture- reverse transcriptionpolymerase chain reaction improve the detection of prunus necrotic ringspot virus in rose. Phytopathology 90, 522–528.
Moury B., Cardin L., Onesto J.P., Candresse T., Poupet, A., 2001. Survey of Prunus necrotic ringspot virus in Rose and its variability in Rose and Prunus spp. Phytopathology 91, 84–91.
Neeleman L., Van der Kuyl A.C., Bol J., 1991. Role of alfalfa mosaic virus coat protein gene in symptom formation. Virology 181, 687–693.
Nolasco G., de Blas C., Torres V., Ponz F., 1993. A method combining immunocapture and PCR amplification in a microtiter plate for the routine diagnosis of plant viruses and subviral pathogens. J. Virol. Meth. 45, 201–218.
Oliver J.E., Freer J., Andersen R.L., Cox K.D., Robinson T.L., Fuchs M., 2009. Genetic diversity of Prunus necrotic ringspot virus isolates within a cherry orchard in New York. Plant Disease 93, 599–606.
Paduch-Cichal E., 2003. First report of occurrence of viruses on some field-grown rose cultivars in Warsaw. Phytopathol. Pol. 28, 53–62.
Paduch-Cichal E., Sala-Rejczak K., Lewko J., 2007. The reaction of Prunus avium, clone F12/1 plants inoculated with PNRSV isolates obtained from different species of Prunus and rose plants. Phytopathol. Pol. 46, 13–23.
Roossinck M.J., Bujarski J., Ding S.W., Hajimorad R., Manada K., Scott S., Tousignant M., 2005. Bromoviridae. W: Virus Taxonomy. Eight Report of the International Committee on Taxonomy of Viruses, Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A. (red.).
Virology Division International Union of Microbiological Societies, Elsevier Academic Press Amsterdam, Boston, Heidelberg, London, New York, Oxford, Paris, San Diego, San Fransisco, Singapore, Sydney, Tokyo, 1049–1058.
Salem N., Mansour A., Al-Musa A., Al-Nsour A., Hammond, R., 2004. Identification and partial characterization of Prunus necrotic ringspot virus on stone fruits in Jordan. J. Plant Pathol. 86, 85–90.
Sanchez-Navarro J.A., Pallas V., 1997. Evolutionary relationships in the ilarviruses: nucleotide sequence of prunus necrotic ringspot virus RNA3. Arch. Virol. 142, 749–763.
Scott S.W., Zimmerman M.T., Ge Xin, MacKenzie D.J., 1998. The coat proteins and putative movement proteins of isolates of Prunus necrotic ringspot virus from different host species and geographic origins are extensively conserved. Europ. J. Plant Pathol. 104, 155–161.
Shintaku M., Zang L., Palukaitis P., 1992. A single amino acid substitution in the coat protein of cucumber mosaic virus induces chlorosis in tobacco. Plant Cell 4, 751–757.
Spiegel S., Holland D., Tam Y., Bar-Yaakov I., Maslein L., Rosner A., 2004. Prunus necrotis ringspot virus isolates in stone fruit germplasm accessions and cultivars in Israel. Ann. Appl. Biol. 144, 229–236.
Spiegel S., Tam Y., Maslein L., Kolber M., Nèmeth M., Rosner A., 1999. Typing Prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products. Ann. Appl. Biol. 135, 395–400.
Thompson J.D., Higgins D.G., Gibson T.J., 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673–4680.
Uemoto J.K., Scott S.W., 1992. Important disease of Prunus caused by viruses and other grafttransmissible pathogens in California and South Carolina. Plant Disease 76, 5–11
Ulubas C., Ertunc F., 2004. RPCR detection and molecular characterizatuion of Prunus necrotic ringspot virus isolaytes occurring in Turkey. J. Phytopathol. 152, 498–502.
Vašková D., Petrzik K., Karešová R., 2000. Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus. Arch. Virol. 145, 699–709.
Wetzel T., Candresse T., Macquire G., Ravelonandro M., Dunez J., 1992. A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. J. Virol. Meth. 39, (1–2), 27–37.
Yurtmen M., Spiegel S., Mastlenin M., Yilmaz M.A., Rosner A., 2002. Assessing the applicability of transcript conformtion polymorphism for differentiation among Prunus necrotic ringspot virus isolates. Phytopahol. Mediterr. 41, 92–98.
Kinga Sala-Rejczak
Warsaw University of Life Sciences
Warsaw University of Life Sciences
Elżbieta Paduch-Cichal
Warsaw University of Life Sciences
Warsaw University of Life Sciences
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