DOES PRIMING PROMOTE GERMINATION AND EARLY STAND ESTABLISHMENT OF FRENCH MARIGOLD (Tagetes patula L.) SEEDS BY INDUCING PHYSIOLOGICAL AND BIOCHEMICAL CHANGES?
Kashif Mukhtar
University of Agriculture, Faisalabad, PakistanIrfan Afzal
University of Agriculture, Faisalabad, PakistanMuhammad Qasim
University of Agriculture, Faisalabad, PakistanShahzad Maqsood Ahmad Basra
University of Agriculture, Faisalabad, PakistanMuhammad Shahid
University of Agriculture, Faisalabad, PakistanAbstract
Poor plant stand establishment is a common problem for marigold production. Exposure to seed priming is one of the strategies being used to accelerate the activation of plant growth, especially seed germination in many crops. Therefore, an experiment was conducted to investigate the influence of different seed priming techniques on performance of French marigold. For this purpose, different seed priming techniques: hydroprimng, 50 mM CaCl2, 100 mg L-1 salicylic acid and 100 mg L-1 ascorbic acid were assessed. Seeds without any treatment were considered as control. Priming with 50 mM
CaCl2 was proved to be the most effective treatment in enhancing germination and seedling vigour among all other treatments including control as shown by higher final germination/emergence, germination energy and lower mean germination/emergence time. Furthermore, positive correlation between reducing sugars and dry weight and α-amylase activity and the concentration of reducing sugars indicates that enhancement due to priming could be attributed to higher reducing and total sugars as well as higher α-amylase activity in primed seeds.
Keywords:
germination, priming, marigold, seedling vigour, stand establishmentReferences
Afzal I., Basra S.M.A., Ahmad N., Cheema M.A., Warraich E.A., Khaliq A., 2002. Effect of priming and growth regulator treatment on emergence and seedling growth of hybrid maize (Zea mays). Int. J. Agric. Biol. 4, 303–306.
Association of Official Seed Analysis AOSA., 1983. Seed Vigour Testing Handbook. Contribution No. 32 to the Handbook on Seed Testing. Assoc. Offic. Seed Analys Springfield IL.
Bosma T.L., Dole J.M., Maness N.O., 2003a. Optimizing marigold (Tagetes erecta L.) petal and pigment yield. Crop. Sci. 43, 2118–2124.
Bosma T.L., Conway K.E., Dole J.M., Maness N.O., 2003b. Sowing dates and priming influence African marigold emergence. HortTechnology 13, 487–493.
Bradford K.J., 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hort. Sci. 21, 1105–1112.
Bray C.M., 1995. Biochemical processes during the osmopriming of seeds. In: Kigel J., Galili G., (Eds). Seed development and germination. New York, Basel, Hong Kong, Maceldekker, pp. 767–789.
Burgass R.W., Powell A.A., 1984. Evidence for repair processes in the invigoration of seeds by hydration. Ann. Bot. 53, 753–757.
Coolbear P., Grierson D., Heydecker W., 1980. Osmotic pre-sowing treatments and nucleic acid accumulation in tomato seeds (Lycopersicon lycoperciscum). Seed Sci. Technol. 8, 289–303.
Corbineau F., Picard M.A., Côme D., 1993. Germinability of some vegetable seeds in relation to temperature and oxygen. In: Côme D, Corbineau F, (Eds.). Fourth International Workshop on
Seeds. Basic and applied aspects of seed biology. ASFIS Paris 3, 1027–1032.
Ellis R.H., Roberts E.H., 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9, 373–409.
Girwani A., Babu R.S., Chandrasekhar R., 1990. Response of marigold (Tagetes erect) to growth regulator and zinc. Indian J. Agric. Sci. 60, 220–222.
Hocart C., Lethem H.D.S., Parker C.W., 1990. Metabolism and translocation of exogenous zeatin riboside in germinating seeds and seedlings of Zea mays. J. Exp. Bot. 41, 1517–1524.
Haghpanah A., Younesi O., Moradi A., 2009. The effect of priming on seedling emergence of differentially matured sorghum (Sorghum bicolor L.) seeds. J. Appl. Sci. Res. 5, 729–732.
Kaul V.K., Singh V., Singh B., 2000. Damask rose and marigold: prospective industrial crops. J. Med. Aroma Plant. Sci. 22, 313–318.
Lee S.S., Kim J.H., 2000. Total sugars, α-amylase activity, and germination after priming of normal and aged rice seeds. Kor. J. Crop. Sci. 45,108–111.
Mirshekari B., 2012. Seed priming with iron and boron enhances germination and yield of dill (Anethum graveolens) Turk. J. Agric. For. 36, 27–33.
Orzesko R.A., Podlaski S., 2003. The effect of sugar beet seed treatment on their vigour. Plant Soil Environ. 49, 249–254.
Sung F.J., Chang Y.H., 1993. Biochemical activities associated with priming of sweet corn seeds to improve vigour. Seed Sci. Technol. 21, 97–105.
Tereschuk M.L., Riera M.V., Castro G.R., Abdala L.R., 1997. Antimicrobial activity of flavonoids from leaves of Tagetes minuta. J. Ethnopharmacol. 56, 227–32.
Thimmaiah S.R., 2004. Standard methods of Biochemical Analysis. Kalyani Publishers, New Dehli, India, pp. 54–55.
Varavinit S., Chaokasem N., Shobsngob S., 2002. Immobilization of a thermostable alphaamylase. Sci. Asia 28, 247–251.
Vasudevan P., Kashyap S., Sharma S., 1997. Tagetes: A multipurpose plant. Centre for Rura1 Development and Appropriate Technology, Indian Institute of Technology, New Delhi-110016, India, pp. 29–35.
University of Agriculture, Faisalabad, Pakistan
University of Agriculture, Faisalabad, Pakistan
University of Agriculture, Faisalabad, Pakistan
University of Agriculture, Faisalabad, Pakistan
University of Agriculture, Faisalabad, Pakistan
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