POT MARIGOLD (Calendula officinalis L.) – A POSITION IN CLASSICAL PHYTOTHERAPY AND NEWLY DOCUMENTED ACTIVITIES

Agnieszka Szopa

Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland

Marta Klimek-Szczykutowicz

Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland

Karolina Jafernik

Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland

Katarzyna Koc

Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland

Halina Ekiert

Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland


Abstract

The flower (inflorescence, basket-type inflorescence) of marigold (Calendulae officinalis flos) is a well-known plant raw material in traditional phytomedicine. Noteworthy is its rich chemical composition, which is dominated by flavonoids and terpenoids. Terpenoids are mainly represented by specific for this species oleanolic acid derivatives: triterpenoid saponins – calendulosides A-H and triterpenoid glycosides, so-called calendulaglycosides A and B. The biological activity profiles of the raw material, such as anti-inflammatory, antimicrobial, immunostimulatory and antioxidant properties, determine its use especially in the treatment of dermatological diseases. Interestingly, recent scientific studies proved, in addition to spasmolitic, hypolipemic and antidiabetic properties, such heath promoting activity as cardioprotective, hepatoregenerative, pancreas regenerating, neuroprotective and even anticancer activities. Moreover, C. officinalis is used in cosmetology and food industry.

Keywords:

marigold, medicinal plant, chemical composition, therapeutic properties, cosmetic application

Aghajanyan, A.A., Trchounian, A.H. (2018). Antihyperglicemic properties of the herbal mixture. Chem. Biol., 52(3), 180–186.

Alexandre, J.T.M., Sousa, L.H.T., Lisboa, M.R.P., Furlaneto, F.A.C., Do Val, D.R., Marques, M., Vasconcelos, H.C., Matos de Melo, I., Leitao, R., Brito G.A.C., Goes, P. (2018). Anti-inflammatory and antiresorptive effects of Calendula officinalis on inflammatory bone loss in rats. Clin. Oral Investig., 22(6), 2175–2185. DOI: 0.1007/s00784-017-2308-7

Anuradha, S.N., Vilashene, G., Lalithambigai, J., Arunkumar, S. (2015). “Cosmeceuticals”: An opinion in the direction of pharmaceuticals. Asian J. Pharm. Clin. Res., 8(2), 64–69.

Arora, D., Rani, A., Sharma, A. (2013). A review on phytochemistry and ethnopharmacological aspects of genus Calendula. Pharmacog. Rev., 7(14), 179. DOI: 10.4103/0973-7847.120520

Bakó, E., Deli, J., Tóth, G. (2002). HPLC study on the carotenoid composition of Calendula products. J. Bioch. Biophys. Methods, 53, 241–250. DOI: 10.1016/S0165-022X(02)00112-4

Banakar, S.M., Veerapur, V.P., Thippeswamy, B.S., Jagadeesh, N.V., Gavimath, C.C., Alshehri, Z.S. (2016). Protective effect of Calendula officinalis (L.) flower extract in acetic acid-induced ulcerative colitis in rats. J. Herbs, Spices Med. Plants, 22(3), 225–237. DOI: 10.1080/10496475.2016.1190952

Basch, E., Bent, S., Foppa, I., Haskmi, S., Kroll, D., Mele, M., Szapary, P., Ulbricht, C., Voa, M., Vora, M., Yong, S. (2006). Marigold (Calendula officinalis L.): an evidence-based systematic review by the Natural Standard Research Collaboration. J. Herb. Pharmacother., 6(3–4), 135–159. DOI: 10.1080/J157v06n03_08

Bingen, H. von (1155). Hildegard von Bingen’s Physica.

Buzzi, M., Freitas, F. de, Barros Winter, M. de (2016). Therapeutic effectiveness of a Calendula officinalis extract in venous leg ulcer healing. J. Wound Care, 25(12), 732–739. DOI: 10.12968/jowc.2016.25.12.732

Colombo, E., Sangiovanni, E., D’Ambrosio, M., Bosisio, E., Ciocarlan, A., Fumagalli, M., Guerriero A., Harghel P., Dell’Agli, M. (2015). A bio-guided fractionation to assess the inhibitory activity of Calendula officinalis L. on the NF-B driven transcription in human gastric epithelial cells. Evid.-Based Complement. Altern. Med., 2, 727342. DOI: 10.1155/2015/727342

Corazza, M., Borghi, A., Gallo, R., Schena, D., Pigatto, P., Lauriola, M.M., Guarneri, F., Stngeni, L., Vincenzi, C., Foti, C., Virgili, A. (2014). Topical botanically derived products: use, skin reactions, and usefulness of patch tests. A multicentre Italian study. Contact Dermatitis, 70(2), 90–97. DOI: 10.1111/cod.12124

Cordova, C., Siqueira, I., Netto, C. (2002). Protective properties of butanolic extract of the Calendula officinalis L. (marigold) against lipid peroxidation of rat liver microsomes and action as free radical scavenger. Redox Rep., 7(2), 95–102. DOI: 10.1179/135100002125000325

CosIng – Cosmetic Ingredient Database. Available: https://ec.europa.eu/growth/sectors/cosmetics/cosing_en [data of access: 06.12.2018].

Denisow-Pietrzyk, M., Pietrzyk, Ł. (2019). Asteraceae species as potential environmental factors of allergy. Environ. Sci. Pollut. Res. 26(1), 1–11. DOI: 10.1007/s11356-019-04146-w

Dinda, M., Dasgupta, U., Singh, N., Bhattacharyya, D., Karmakar, P. (2015). PI3K-Mediated proliferation of fibroblasts by Calendula officinalis tincture: Implication in wound healing. Phytother. Res., 29(4), 607–616. DOI: 10.1002/ptr.5293

Duran, V., Matic, M., Jovanovic, M. (2005). Results of the clinical examination of an ointment with marigold (Calendula officinalis) extract in the treatment of venous leg ulcers. Int. J. Tissue. React., 27(3), 101–106.

EFSA – European Food Safety Authority. Available: http://www.efsa.europa.eu/ [data of access: 11.11.2018].

European Pharmacopoeia 9.0 (2017). European Directorate for the Quality of Medicines, Strasburg.

EMA – European Medicines Agency. Available: https://www.ema.europa.eu/ [data of access: 06.01.2019].

ESCOP – European Scientific Cooperative on Phytotherapy. Available: http://escop.com/ [data of access: 20.02.2019].

Farahpour, M.R. (2014). Antioxidant activity, antinociceptive and anti-inflammatory effects of pot marigold hydroalcoholic extract on experimental animals. Int.

J. PharmTech. Res., 6(5), 1640–1646.

Farmakopea Polska XI (2017). Urząd Rejestracji Produktów Leczniczych, Wyrobów Medycznych i Produktów Biobójczych, Warszawa.

Faustino, M.V., Pinto, D.C.G.A., Gonçalves, M.J., Salgueiro, L., Silveira, P., Silva, A.M.S. (2018). Calendula L. species polyphenolic profile and in vitro antifungal activity. J. Funct. Foods, 45, 254–267. DOI: 10.1016/j.jff.2018.04.013

Georgiev, V., Slavov, A., Vasileva, I., Pavlov, A. (2018). Plant cell culture as emerging technology for production of active cosmetic ingredients. Eng. Life Sci., 18(11), 779–798. DOI: 10.1002/elsc.201800066

German Commission E Monographs, Phytotherapy (1986). Calendula flower (Calendulae flos) [summary of the monograph]. Available: https://buecher.heilpflanzen-welt.de/BGA-Commission-E-Monographs/0043.htm [data of access: 26.01.2019].

Gilca, M., Tiplica, G.S., Salavastru, C.M. (2018). Traditional and ethnobotanical dermatology practices in Romania and other Eastern European countries. Clin. Dermatol., 36(3), 338–352, DOI: 10.1016/j.clindermatol.2018.03.008

Grzelak, A., Janiszowska, W. (2002). Initiation and growth characteristics of suspension cultures of Calendula officinalis cells. Plant Cell. Tissue Organ Cult., 71, 29–40. DOI: 10.1023/A:1016553909002

Hernández-Saavedra, D., Pérez-Ramírez, I.F., Ramos-Gómez, M., Mendoza-Díaz, S., Loarca-Piña, G., Reynoso-Camacho, R. (2016). Phytochemical characterization and effect of Calendula officinalis, Hypericum perforatum, and Salvia officinalis infusions on obesity-associated cardiovascular risk. Med. Chem. Res., 25(1), 163–172. DOI: 10.1007/s00044-015-1454-1

Jadoon, S., Karim, S., Hassham, M., Bin, H., Akram, M.R., Khan, A.K., Malik, A., Chen, C., Murtaza, G. (2015). Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity. Oxidative Med. Cell. Longev., 2015, 1–17. DOI: 10.1155/2015/709628

John, R., Andrabi, K.I., Jan, N. (2017). Calendula officinalis – an important medicinal plant with potential biological properties. Proc. Indian Natl. Sci. Acad., 83(4), 769–787. DOI: 10.16943/ptinsa/2017/49126

Kaur, J., Sidhu, S., Chopra, K., Khan, M.U. (2016). Calendula officinalis ameliorates l-arginine-induced acute necrotizing pancreatitis in rats. Pharm. Biol., 54(12), 2951–2959. DOI: 10.1080/13880209.2016.1195848

Khalid, K.A., Silva, J.A.T. (2012). Biology of Calendula officinalis Linn.: Focus on pharmacology, biological activities and agronomic practices. Med. Aromat. Plant Sci. Biotechnol. 6, 12–27.

Kurkin, V.A., Sharova, O.V. (2007). Flavonoids from Calendula officinalis flowers. Chem. Nat. Compd., 43(2), 216–217. DOI: 10.1007/s10600-007-0084-3

Leach, J.M. (2008). Calendula officinalis and wound healing: a systematic review. Wounds, 20(8), 236–243.

Legha, M.R., Prasad, K.V., Singh, S.K., Kaur, C., Arora, A., Kumar, S. (2012). Induction of carotenoid pigments in callus cultures of Calendula officinalis L. in response to nitrogen and sucrose levels. Vitr. Cell. Dev. Biol. – Plant, 48(1), 99–106. DOI: 10.1007/s11627-011-9402-3

Lima, M.R., Lopes, A.P., Martins, C., Brito, G.A.C., Carneiro, V.C., Goes, P. (2017). The effect of Calendula officinalis on oxidative stress and bone loss in experimental periodontitis. Front. Physiol., 8, 1–9. DOI: 10.3389/fphys.2017.00440

Lohani, A., Mishra, A.K., Verma, A. (2018). Cosmeceutical potential of geranium and calendula essential oil: Determination of antioxidant activity and in vitro sun protection factor. J. Cosmet. Dermatol., 1–8. DOI: 10.1111/jocd.12789

Lovecka, P., Lipov, J., Thumova, K., Macurkova, A. (2018). Characterization of biologically active substances from Calendula officinalis. Curr. Pharm. Biotechnol., 19, 1167–1174. DOI: 10.2174/1389201019666180226151910

Miguel, M., Barros, L., Pereira, C., Calhelha, R.C., Garcia, P.A., Castro, M.Á., Santos-Buelga, C., Ferreira, I.C.F.R. (2016). Chemical characterization and bioactive properties of two aromatic plants: Calendula officinalis L. (flowers) and Mentha cervina L. (leaves). Food Funct., 7(5), 2223–2232. DOI: 10.1039/c6fo00398b

Muley, B.P., Khadabadi, S.S., Banarase, N.B. (2009). Phytochemical constituents and pharmacological activities of Calendula officinalis Linn (Asteraceae): A review. Trop. J. Pharm. Res., 8(5), 455–465. DOI: 10.4314/tjpr.v8i5.48090

Neukirch, H., D’Ambrosio, M., Dalla Via, J., Guerriero, A. (2004). Simultaneous quantitative determination of eight triterpenoid monoesters from flowers of 10 varieties of Calendula officinalis L. and characterisation of a new triterpenoid monoester. Phytochem. Anal., 15(1), 30–35. DOI: 10.1002/pca.739

Nicolaus, C., Junghanns, S., Hartmann, A., Murillo, R., Ganzera, M., Merfort, I. (2017). In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts. J. Ethnopharmacol., 196, 94–103. DOI: 10.1016/j.jep.2016.12.006.

Nizyński, B., Alsoufi, A.S.M., Pączkowski, C., Długosz, M., Szakiel, A. (2015). The content of free and esterified triterpenoids of the native marigold (Calendula officinalis) plant and its modifications in in vitro cultures. Phytochem. Lett., 11, 410–417. DOI: 10.1016/j.phytol.2014.12.017

Okoh, O.O., Sadimenko, A.A., Afolayan, A. (2007). The effects of age on the yield and composition of the essential oils of Calendula officinalis. J. Appl. Sci., 7(23), 3806–3810. DOI: 10.3923/jas.2007.3806.3810

Okuma, C.H., Andrade, T.A.M., Caetano, G.F., Finci, L.I., Maciel, N.R., Topan, J.F., Cefali, L.C., Polizello, A.C.M., Carlo, T., Rogerio, A.P., Spadaro, A.C.C., Isaac, V.L.B., Frade, M.A.C., Rocha-Filho, P.A. (2015). Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing. Eur. J. Pharm. Sci., 71, 62–72. DOI: 10.1016/j.ejps.2015.01.016

Parente, L.M.L., Souza Lino Jr, R. de, Tresvenzol, L.M.F., Vinaud, M.C., Paula, J.R. de, Paulo, N.M. (2012). Wound healing and anti-inflammatory effect in animal models of Calendula officinalis L. growing in Brazil. Evid.-Based Complement. Altern. Med., 2012, 1–7. DOI: 10.1155/2012/375671

Paul, S., Rahman, A., Mahmud, M., Uzzal, A., Das, Z. (2018). Wound healing by marigold (Calendula officinalis) and turmeric (Curcuma longa) Paste: a comparative approach. J. Adv. Vet. Anim. Res., 4(4), 333. DOI: 10.5455/javar.2017.d229

Pedram Rad, Z., Mokhtari, J., Abbasi, M. (2019). Preparation and characterization of Calendula officinalis-loaded PCL/gum arabic nanocomposite scaffolds for wound healing applications. Iran. Polym. J., 28(1), 51–63. DOI: 10.1007/s13726-018-0674-x

Pommier, P., Gomez, F., Sunyach, M.P., D’Hombres, A., Carrie, C., Montbarbon, X. (2004). Phase III randomised trial of Calendula officinalis compared with trolamine for the prevention of acute dermatitis during irradiation for breast cancer. J. Clin. Oncol., 22(8), 1447–1453. DOI: 10.1200/JCO.2004.07.063

Preethi, K.C., Kuttan, R. (2009). Wound healing activity of flower extract of Calendula officinalis. J. Basic Clin. Physiol. Pharmacol., 20(1), 73–79. DOI: 10.1515/jbcpp.2009.20.1.73

Reider, N., Komericki, P., Hausen, B.M., Fritsch, P., Aberer, W. (2001). The seamy side of natural medicines: Contact sensitization to arnica (Arnica montana L.) and marigold (Calendula officinalis L.). Contact Dermatitis, 45(5), 269–272. DOI: 10.1034/j.1600-0536.2001.450503.x

Ruszkowski, D., Szakiel, A., Janiszowska, W. (2005). Metabolism of [3-3H]oleanolic acid in Calendula officinalis L. root. Acta Physiol. Plant., 25(4), 311–317. DOI: 10.1007/s11738-003-0011-6

Saffari, E., Mohammad-Alizadeh-Charandabi, S., Adibpour, M., Mirghafourvand, M., Javadzadeh, Y. (2017). Comparing the effects of Calendula officinalis and clotrimazole on vaginal Candidiasis: A randomized controlled trial. Women Heal., 57(10), 1145–1160. DOI: 10.1080/03630242.2016

Salehi, B., Anil Kumar, N.V., Şener, B., Sharifi-Rad, M., Kılıç, M., Mahady, G.B., Mehtap, K., Vlaicavljevic, S., Iriti, M., Kobarfard, F., Setzer, W.N., Ayatollahi, S.A., Ata, A., Sharifi-Rad, J. (2018). Medicinal plants used in the treatment of human immunodeficiency virus. Int. J. Mol. Sci., 19(5), 1459. DOI: 10.3390/ijms19051459

Samochowiec, E., Urbanska, L., Manka, W., Stolarska, E. (1979). Assessment of the action of Calendula officinalis and Echinacea angustifolia extracts on Trichomonas vaginalis in vitro. Wiad. Parazytol., 25(1), 77–81.

Shafeie, N., Naini, A.T., Jahromi, H.K. (2015). Comparison of different concentrations of Calendula officinalis gel on cutaneous wound healing. Biomed. Pharmacol. J., 8(2), 979–992. DOI: 10.13005/bpj/850

Shankar, S.M., Bardvalli, S.G., Jyotirmayee, R., Chethana, Bhushan, K., Kumar, S. (2017). Efficacy of Calendula officinalis extract (marigold flower) as an antimicrobial agent against oral microbes: An in vitro study in comparison with chlorhexidine digluconate. J. Clin. Diagnostic Res., 11(10), ZC05-ZC10. DOI: 10.7860/JCDR/2017/29844.10702

Shivasharan, B.D., Nagakannan, P., Thippeswamy, B.S., Veerapur, V.P., Bansal, P., Unnikrishnan, M.K. (2013). Protective effect of Calendula officinalis Linn. flowers against 3-nitropropionic acid induced experimental Huntington’s disease in rats. Drug Chem. Toxicol., 36(4), 466–473. DOI: 10.3109/01480545.2013.776583

Singh, M.K., Sahu, P., Nagori, K., Dewangan, D., Alexander, T.K.A., Badwaik, H., Tripathi, D.K. (2011). Organoleptic properties in-vitro and in-vivo pharmacological activities of Calendula officinalis Linn.: An over review. J. Chem. Pharm. Res., 3(4), 655–663.

Toropova, A.A., Badmaev, N.S., Razuvaeva, Y.G., Nikolaev, S.M., Sambueva, Z.G., Erentueva, A. (2017). Influence of Calendula officinalis extract on the antioxidant and energy status of liver in rats with experimental hepatitis. Eksp. Klin. Farmakol., 80(7), 11–14.

Tundis, R., Loizzo, M.R., Bonesi, M., Menichini, F. (2015). Potential role of natural compounds against skin aging. Curr. Med. Chem., 22(12), 1515–1538. DOI: 10.2174/0929867322666150227151809

FDA – U.S. Food & Drug Administration. Available: https://www.fda.gov/ [data of access: 22.01.2019].

Ukiya, M., Akihisa, T., Yasukawa, K., Tokuda, H., Suzuki, T., Kimura, Y. (2006). Anti-inflammatory, anti-tumor-promoting, and cytotoxic activities of constituents of Marigold (Calendula officinalis) flowers. J. Nat. Prod., 69(12), 1692–1696. DOI: 10.1021/np068016b

WHO (2002). Monographs on selected medical plants,

vol. 2. Geneva.

Wiktorowska, E., Długosz, M., Janiszowska, W. (2010). Significant enhancement of oleanolic and accumulation by biotic elicitors in cell suspension cultures of Calendula officinalis L. Enzyme Microb. Technol., 46, 14–20. DOI: 10.1016/j.enzmictec.2009.09.002

Xuan, S.H., Kim, G.Y., Yu, J.Y., Kim, J.W., Yang, Y.R., Jeon, Y.H., Jeong, Y.J., Kim, R.A., Park, S.N. (2016). Antioxidant and cellular protective effects against oxidative stress of Calendula officinalis flowers extracts in human skin cells. Appl. Chem. Eng., 27(6), 620–626. DOI: 10.14478/ace.2016.1093

Xuan, S.H., Park, Y.M., Park, S.N. (2019). Antimelanogenic and antimigration properties of the ethyl acetate fraction of Calendula officinalis flowers on melanoma cells. Photochem. Photobiol., 95(3), 860–866. DOI: 10.1111/php.13064

Download

Published
2020-06-29



Agnieszka Szopa 
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland
Marta Klimek-Szczykutowicz 
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland
Karolina Jafernik 
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland
Katarzyna Koc 
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland
Halina Ekiert 
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, ul. Medyczna 9, 30-688 Kraków, Poland



License

 

Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.

 


Most read articles by the same author(s)