THE EFFECT OF AUXINS: IAA, IBA AND NAA ON ROOTING OF Hebe buchananii (HOOK) AND Hebe canterburiensis (J.B.ARMSTR.) ‘PROSTRATA’ IN VITRO
Marek Dąbski
Agricultural University in LublinMarzena Parzymies
Agricultural University in LublinAbstract
The effect of auxins: IAA, IBA and NAA in concentrations of 1.0, 2.5 and 5.0 mg·dm-3 on rooting of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro
was examined. Shoots used in the experiment were excised from aseptically grown shoots on 1/2 MS media. Auxins used in the experiment were showed to have a positive effect on rooting of shoots of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro. The biggest number of rooted microshoots and the longest roots of Hebe buchananii formed in presence of IBA. On the media supplemented with IAA in concentrations of 2.5 and 5.0 mg·dm-3, IBA in concentration of 5 mg·dm-3 and NAA in concentration of 1 mg·dm-3 100% of rooted shoots of Hebe canterburiensis ‘Prostrata’ were obtained. The biggest number of roots occurred on media with 2.5 and 5.0 mg IAA·dm-3. NAA added to the media caused callusing of the shoots base.
Keywords:
Hebe, auxins, rooting, in vitroReferences
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Agricultural University in Lublin
Agricultural University in Lublin
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