Investigating the impact of TiO2 nanoparticles on bioactive compounds in sweet pepper seedlings: a comparison of foliar and root application methods
Andrzej Kalisz
Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Polandhttps://orcid.org/0000-0002-8437-9307
Andrzej Kornaś
Institute of Biology and Earth Sciences, Pedagogical University of Krakow, Podchorążych 2, 30‐084 Kraków, Polandhttps://orcid.org/0000-0001-9945-3322
Dalibor Húska
Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republichttps://orcid.org/0000-0003-3852-8751
Radim Zelinka
Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republichttps://orcid.org/0000-0001-9591-1330
Agnieszka Sękara
Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Polandhttps://orcid.org/0000-0002-9655-5742
Robert Pokluda
Department of Vegetable Sciences and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republichttps://orcid.org/0000-0003-0492-6401
Andrzej Sałata
Department of Vegetable and Medicinal Plants, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Polandhttps://orcid.org/0000-0002-8162-6587
Joanna Gil
Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Polandhttps://orcid.org/0000-0001-9801-7983
Abstract
Engineered TiO2 nanoparticles (TiO2-NPs) are broadly produced and utilized in various consumer products. However, plant uptake of NPs may lead to disruptions in physiological and metabolic processes, particularly when the plant’s defense mechanisms are overwhelmed. In this study, sweet pepper seedlings were exposed to TiO2-NPs via foliar (2.5% suspension) and root (0.5% suspension) methods, with plants treated with distilled water serving as controls. Results showed that foliar application caused higher accumulation of Ti in leaves as compared to stems, while root exposure led to a higher increase of Ti content in stems than in leaves. Additionally, foliar application led to alterations in chemical composition of the plants, including changes in malondialdehyde (MDA), L-ascorbic acid, total phenolics content, carotenoids, in total antioxidant capacity (TAC) and antioxidant enzymes activity. Root exposure also affected enzyme activity and TAC, but also altered H2O2, MDA and glutathione content. Chlorophylls remained at stable level in the leaves of the seedlings. Overall, these studies provide important information on plant-nanoparticle interactions and the potential effects of different nanoparticle application strategies. These data indicate also that the specific nanoparticles, applied at a controlled manner, have potential to boost the plant metabolism and improve stress tolerance, which is an important factor affecting crops’ quality and productivity.
Keywords:
absorption pathway, antioxidants, Capsicum annuum L., nanoparticles, titaniumReferences
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Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Poland https://orcid.org/0000-0002-8437-9307
Institute of Biology and Earth Sciences, Pedagogical University of Krakow, Podchorążych 2, 30‐084 Kraków, Poland https://orcid.org/0000-0001-9945-3322
Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic https://orcid.org/0000-0003-3852-8751
Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic https://orcid.org/0000-0001-9591-1330
Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Poland https://orcid.org/0000-0002-9655-5742
Department of Vegetable Sciences and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic https://orcid.org/0000-0003-0492-6401
Department of Vegetable and Medicinal Plants, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland https://orcid.org/0000-0002-8162-6587
Department of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, Poland https://orcid.org/0000-0001-9801-7983
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