Chromosomal localization of genes encoding small heat shock proteins (HSPB) in cattle and sheep
BARBARA DANIELAK-CZECH
1Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówMAREK BABICZ
2Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 LublinAbstract
The small heat shock protein gene products (HSPB) reveal chaperone and neuroprotective activity contributing to the stabilization of cell homeostasis and cytoskeleton of neurons in conditions of thermal or oxidative stress. The aim of this study was chromosomal localization of five small heat shock protein genes (HspB1, HspB2, CRYAB – alternative name HspB5, HspB6 and HspB8) in cattle and sheep, with an application of FISH technique with the probes obtained from BAC clones (derived from the CHORI-240 Bovine BAC Library) containing sequences of these genes. Prior to in situ hybridization, carried out on metaphase chromosomes stained by means of DAPI bands technique, the presence of the studied genes in the selected clones was confirmed by PCR method with the use of the gene-specific primers. As a result of the experiments, FISH signals in the following cattle (BTA) and sheep (OAR) genome regions were obtained: BTA25q22/OAR24q22 (HSPB1), BTA15q14-21/OAR15q14-21 (HSPB2 and CRYAB), BTA18q24/OAR14q24 (HSPB6), BTA17q24-25/OAR17q24-25 (HSPB8). The studies enabled to designate physical localization of the studied genes on the genome maps of these species and confirmed the high level of autosome conservation in Bovidae. The results obtained may provide useful information concerning the genetic background of neurodegenerative diseases in breeding animals.
Keywords:
cattle and sheep chromosomes, FISH technique, cytogenetic mapping, small heat shock proteins – HSPB, neurodegenerative disordersReferences
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Schibler L., Di Meo G.P., Iannuzzi L., 2009. Molecular cytogenetics and comparative mapping in goats (Capra hircus, 2n = 60). Cytogenet. Genome Res. 126, 77–85.
Serrano C., Bolea R., Lyahyai J., Filali H., Varona L., Marcos-Carcavilla A., Acin C., Calvo J.H., Serrano M. Badiola J.J., 2011. Changes in HSP gene and protein expression in natural scrapie with brain damage. Vet. Res. 42: 13, doi: 10.1186/1297-9716-42-13.
Tortosa R., Vidal E, Costa C., Alamillo E., Torres J.M., Ferrer I., Pumarola M., 2008. Stress response in the central nervous system of a transgenic mouse model of bovine spongiform encephalopathy. Vet. J. 178, 126–129.
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Wettstein G., Bellaye P.S., Micheau O., Bonniaud P., 2012. Small heat shock proteins and the cytoskeleton: An essential interplay for cell integrity? Int. J. Biochem. Cell B. 44(10), 1680–1686.
Zhang C., de Koning D.J., Hernandez-Sanchez J., Haley C.S., Williams J.L., Wiener P., 2004. Mapping of multiple quantitative trait loci affecting bovine spongiform encephalopathy. Genetics 167, 1863–1872.
Arrigo A.P., 2012. Pathology–dependent effects linked to small heat shock proteins expression: an update. Scientifica 185641, doi: org/10.6064/2012/185641.
Arrigo A.P., 2013. Human small heat shock proteins: Protein interactomes of homo- and heterooligomeric complexes: An update. FEBS Lett. 587, 1959–1969.
Bae S.E., Jung S, Kim H.Y., Son H.S., 2012. Correlation analysis for the incubation period of prion disease. Prion 6, 276–281.
Boncoraglio A., Minoia M., Carr S., 2012. The family of mammalian small heat shock proteins (HSPBs): Implications in protein deposit diseases and motor neuropathies. Int.l J. Biochem. Cell Biol. 44, 1657–1669.
Brown C.A., Schmidt C., Poulter M., Hummerich H., Klӧhn P.C., Jat P., Mead S., Collinge J., Lloyd S.E., 2014. In vitro screen of prion disease susceptibility genes using the scrapie cell assay. Hum. Mol. Genet. 2, 5102–5108.
Brownell S.E., Becker R.A., Steinman L., 2012. The protective and therapeutic function of small heat shock proteins in neurological diseases. Front. Immunol. 3, 74, doi: 10.3389/fimmu. 2012.00074.
Chowdhary B.P., Fronicke L., Gustavsson I., Scherthan H., 1996. Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mamm. Genome 7, 297–302.
Danielak-Czech B., Kozubska-Sobocińska A., Bąk A., 2014a. FISH-based comparative mapping of the Hsp27 gene on chromosomes of the domestic Bovids. Chromosome Res. 22, 414–414.
Danielak-Czech B., Kozubska-Sobocińska A., Kruczek K., 2014b. Chromosomal assignment of the small heat protein genes in the sheep genome. Chrom. Res. 22, 412–413.
Darlymple B.P., Kirkness E.F., Nefedov M., McWillam S., Ratnakumar A., Barris W., Zhao S., Shetty J., Maddox J.F., O’Grady M., Nicholas F., Crawford A.M., Smith T., de Jong P..J, McEwan J., Oddy V.H., Cockett N.E., International Sheep Genomics Consortium, 2007. Using comparative genomics to reorder the human genome sequence into a virtual sheep genome. Genome Biol. 8, R152, doi: 10.1186/gb-2007-8-7-r152.
De Lorenzi L., Molteni L. Parma P., 2010. FISH mapping in cattle (Bos taurus L.) is not yet of fashion. J. Appl. Genet. 51, 497–499.
Di Berardino D., Di Meo G.P., Gallagher D.S., Hayes H., Iannuzzi L., 2001. International system for chromosome nomenclature of domestic bovids ISCNDB 2000. Cytogenet. Cell Genet. 92, 283–299.
Everts-van der Wind A., Kata S.R., Band M.R., Rebeitz M., Larkin D.M., Everts R.E., Green C.A., Liu L., Natarajan S., Goldammer T., Lee J.H., McKay S., Womack J.E., Lewin H.A., 2004. A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Res. 14, 1424–1437.
Goldammer T., Di Meo G.P., Lühken G., Drӧgemüller C., Wu C.H., Kijas J., Dalrymple B.P., Nicholas F.W., Maddox J.F., Iannuzzi L., Cockett N.E., 2009. Molecular cytogenetics and gene mapping in sheep (Ovis aries, 2n = 54). Cytogenet. Genome Res. 126, 63–76.
Hernandez-Sanchez J., Waddington D., Wiener P., Haley C.S., Williams J.L., 2002. Genome-wide search for markers associated with bovine spongiform encephalopathy. Mamm. Genome 13, 164–168.
Hu Z.L., Park C.A., Wu X.L., Reccy J.M., 2013. Animal QTLdb: an improved database tool for livestock animal QTL/association data dissemination in the post-genome era. Nucleic Acids Res. 41, 871–879, doi: 10.1093/nar/gks1150.
Iannuzzi L. Di Berardino D., 2008. Tools of the trade: diagnostics and research in domestic animal cytogenetics. J. Appl. Genet. 49, 357–366.
Iannuzzi L., King W.A., Di Berardino D., 2009. Chromosome evolution in domestic bovids as revealed by chromosome banding and FISH-mapping techniques. Cytogenet. Genome Res. 126, 49–62.
Itoch T., Watanabe T., Ihara N., Mariani P., Beattie C.W., Sugimoto Y., Takasuga A., 2005. A comprehensive radaition hybrid map of the genome comprising 5593 loci. Genomics 85, 413–424.
Iwaki A., Nagano T., Nakagawa M., Iwaki T., Fukumaki Y., 1997. Identification and characterization of the gene encoding a new member of the alpha-crystallin/small hsp family, closely linked to the alphaB-crystallin gene in a head-to-head manner. Genomics 45, 386–94.
Lewin H., Larkin D.M., Pontius J., O’Brien S.J., 2009. Every genome sequence needs a good map. Genome Res. 19, 1925–1928.
Marcos-Carcavilla A., Calvo J.H., Gonzáles C., Moazami-Goudarzi K., Laurent P., Bertaud M., Hayes H., Beattie A.E., Serrano C.,Lyahyai J., Martin-Burriel I., Serrano M., 2008. Structural and functional analysis of the HSP90AA1 gene: distribution of polymorphisms among sheep with different responses to scrapie. Cell Stress Chaperone 13, 19–29.
Marcos-Carcavilla A., Moreno C., Serrano M., Laurent P., Cribiu E.P., Andreoletti O., Ruesche J., Weisbecker J.L., Calvo J.H., Moazami-Goudarzi K., 2010. Polymorphisms in the HSP90AA1 5’ flanking region are associated with scrapie incubation period in sheep. Cell Stress Chaperone 15, 343–349.
Moreno C.R., Cosseddu G.M., Schibler L., Roig A., Moazami-Goudarzi K., Andreoletti O., Eychenne F., Lajous D., Schelcher F., Cribiu E.P., Laurent P., Vaiman D., Elsen J.M., 2008. Identification of new quantitative trait loci (other than the PRNP gene) modulating the scrapie incubation period in sheep. Genetics 179, 723–726.
Moreno C.R., Moazami-Goudarzi K., Briand S., Robert-Granié C., Weisbecker J.L., Laurent P., Cribiu E.P., Haley C.S., Andreoletti O., Bishop S.C., Pong-Wong R., 2010. Mapping of quantitative trait loci affecting classical scrapie incubation time in a population comprising several generations of scrapie-infected sheep. J. Gen. Virol. 91, 575–579.
Sawiris G.P., Becker K.G., Elliott E.J., Moulden R., Rohwer R.G., 2007. Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays. J. Gen. Virol. 88, 1356–1362.
Schibler L., Di Meo G.P., Iannuzzi L., 2009. Molecular cytogenetics and comparative mapping in goats (Capra hircus, 2n = 60). Cytogenet. Genome Res. 126, 77–85.
Serrano C., Bolea R., Lyahyai J., Filali H., Varona L., Marcos-Carcavilla A., Acin C., Calvo J.H., Serrano M. Badiola J.J., 2011. Changes in HSP gene and protein expression in natural scrapie with brain damage. Vet. Res. 42: 13, doi: 10.1186/1297-9716-42-13.
Tortosa R., Vidal E, Costa C., Alamillo E., Torres J.M., Ferrer I., Pumarola M., 2008. Stress response in the central nervous system of a transgenic mouse model of bovine spongiform encephalopathy. Vet. J. 178, 126–129.
Vidal E., Acín C., Foradada L, Monzόn M., Márquez M., Monleon E., Pumarola M., Badiola J.J., Bolea R., 2009. Immunohistochemical characterization of classical scrapie neuropathology in sheep. J. Comp. Pathol. 141, 135–146.
Wettstein G., Bellaye P.S., Micheau O., Bonniaud P., 2012. Small heat shock proteins and the cytoskeleton: An essential interplay for cell integrity? Int. J. Biochem. Cell B. 44(10), 1680–1686.
Zhang C., de Koning D.J., Hernandez-Sanchez J., Haley C.S., Williams J.L., Wiener P., 2004. Mapping of multiple quantitative trait loci affecting bovine spongiform encephalopathy. Genetics 167, 1863–1872.
BARBARA DANIELAK-CZECH
1Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
1Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
ANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
MAREK BABICZ
2Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
2Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
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