Genetic diversity of Ukrainian Charolais cattle assessed by selected genetic markers
NATALYA SHKAVRO
1 National Academy of Agrarian Sciences of Ukraine, Institute of Animal Science, Kharkov, UkraineANNA RADKO
2 Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, PolandANNA KOZUBSKA-SOBOCIŃSKA
2 Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, PolandTYMOFII TKACHYK
1 National Academy of Agrarian Sciences of Ukraine, Institute of Animal Science, Kharkov, UkraineAbstract
This article presents the results of a study on 10 microsatellite DNA loci polymorphism in Charolais cattle (n = 101). Seventy-seven allelic variants were identified and the largest number of alleles was observed for TGLA53 locus (12 alleles). All microsatellite DNA markers, except ETH10, showed a high degree of polymorphism. A significantly high B allele frequency (0.269) of kappa-casein gene was detected and a large number of animals (0.508) have the heterozygous AB genotype variant. The frequency of V allele (0.511) of the growth hormone gene was non-significantly higher than that of L allele (0.489). Animals with heterozygous LV genotype prevailed (0.537); frequencies of homozygous LL and VV genotypes were 0.221 and 0.242, respectively.
Keywords:
DNA microsatellite loci, kappa-casein gene, growth hormone gene, polymorphism, CharolaisReferences
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1 National Academy of Agrarian Sciences of Ukraine, Institute of Animal Science, Kharkov, Ukraine
2 Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland
2 Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 32-083 Balice, Poland
1 National Academy of Agrarian Sciences of Ukraine, Institute of Animal Science, Kharkov, Ukraine
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