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ASPHC Baner

Vol. 25 No. 1 (2026)

Articles

Mitigation of salinity stress on pistachio (Pistacia vera L.) seedlings through the application of carbon nitride modified with iron (Fe/C3N4) nanostructures

DOI: https://doi.org/10.24326/asphc.2026.5540
Submitted: 1 May 2025
Published: 27.02.2026

Abstract

Salinity has a global impact on plants by inducing biochemical and metabolic changes that lead to oxidative stress, impairing growth, yield, and productivity. The pistachio tree (Pistacia vera L.) is a salt-tolerant species. This study aims to investigate the effectiveness of carbon nitride nanostructures modified with iron (Fe/C₃N₄) on the Akbari pistachio variety under salinity stress levels (0, 50, 100, and 150 mM) and foliar applying (distilled water as a control, Fe₂O₃ (0.2 g L–1), C₃N₄ (0.2 g L–1), and carbon nitride modified with iron or Fe/C₃N₄ (0.2 g L–1). The findings showed that salinity decreased relative water content (RWC), SPAD index, membrane stability index (MSI), maximum fluorescence (Fm), and variable fluorescence (Fv), and increased hydrogen peroxide (H2O2). However, foliar application with Fe₂O₃, or Fe/C₃N₄, improved all traits. Nevertheless, there was no significant interaction between the applied mitigating treatments and salinity levels on RWC, MSI, SPAD index, Fm, Fv, and H₂O₂. Salinity stress increased malondialdehyde (MDA), phenol, and flavonoid levels, and reduced leaf number, height, photosynthetic pigments, vitamin C, and total protein. The application of foliar treatments, especially Fe/C₃N₄, improved the influence of salinity stress. Additionally, the activity of antioxidant enzymes increased under salt stress and foliar application. Fe/C₃N₄-treated seedlings consistently exhibited higher growth and photosynthetic traits and lower oxidative damage than untreated controls across salinity levels, indicating a stable physiological benefit rather than a salinity-specific effect.

References

  1. Abdoli, S., Ghassemi-Golezani, K., Alizadeh-Salteh, S. (2020). Responses of ajowan (Trachyspermum ammi L.) to exogenous salicylic acid and iron oxide nanoparticles under salt stress. Environ. Sci. Pollut. Res., 27(29), 36939–36953. https://doi.org/10.1007/s11356-020-09453-1 DOI: https://doi.org/10.1007/s11356-020-09453-1
  2. Ahmadi, M., Souri, M.K. (2018). Growth and mineral content of coriander (Coriandrum sativum L.) plants under mild salinity with different salts. Acta Physiol. Plant., 40(11), 194. https://doi.org/10.1007/s11738-018-2773-x DOI: https://doi.org/10.1007/s11738-018-2773-x
  3. Ahmadi, M., Souri, M.K. (2020). Growth characteristics and fruit quality of chili pepper under higher electrical conductivity of nutrient solution induced by various salts. AGRIVITA J. Agric. Sci., 42(1), 143–152. https://doi.org/http://doi.org/10.17503/agrivita.v42i1.2225 DOI: https://doi.org/10.17503/agrivita.v42i1.2225
  4. Ahmed, I.M., Cao, F., Zhang, M. et al. (2013). Difference in yield and physiological features in response to drought and salinity combined stress during anthesis in Tibetan wild and cultivated barleys. PloS One, 8(10), e77869. https://doi.org/https://doi.org/10.1371/journal.pone.0077869 DOI: https://doi.org/10.1371/journal.pone.0077869
  5. Akhavan, A., Gonçalves, P. (2021). Managing the trade-off between groundwater resources and large-scale agriculture: the case of pistachio production in Iran. Syst. Dyn. Rev., 37(2–3), 155–196. https://doi.org/https://doi.org/10.1002/sdr.1689 DOI: https://doi.org/10.1002/sdr.1689
  6. Akrami, M., Arzani, A. (2018). Physiological alterations due to field salinity stress in melon (Cucumis melo L.). Acta Physiol. Plant., 40(5), 91. https://doi.org/10.1007/s11738-018-2657-0 DOI: https://doi.org/10.1007/s11738-018-2657-0
  7. Al-Dossary, O., Alnaddaf, L.M., Al-Khayri, J.M. (2025). Crop management to enhance plant resilience to abiotic stress using nanotechnology: towards more efficient and sustainable agriculture. Front. Plant Sci., 16. https://doi.org/10.3389/fpls.2025.1626624 DOI: https://doi.org/10.3389/fpls.2025.1626624
  8. Alam, M.S., Tester, M., Fiene, G. et al. (2021). Early growth stage characterization and the biochemical responses for salinity stress in tomato. Plants, 10(4), 712. https://doi.org/10.3390/plants10040712 DOI: https://doi.org/10.3390/plants10040712
  9. Ali, Q., Daud, M.K., Haider, M.Z. et al. (2017). Seed priming by sodium nitroprusside improves salt tolerance in wheat (Triticum aestivum L.) by enhancing physiological and biochemical parameters. Plant Physiol. Biochem., 119, 50–58. https://doi.org/10.1016/j.plaphy.2017.08.010 DOI: https://doi.org/10.1016/j.plaphy.2017.08.010
  10. An, C., Sun, C., Li, N. et al. (2022). Nanomaterials and nanotechnology for the delivery of agrochemicals: strategies towards sustainable agriculture. J. Nanobiotechnol., 20(1), 11. https://doi.org/10.1186/s12951-021-01214-7 DOI: https://doi.org/10.1186/s12951-021-01214-7
  11. Apel, K., Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol., 55(1), 373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701 DOI: https://doi.org/10.1146/annurev.arplant.55.031903.141701
  12. Arif, Y., Singh, P., Siddiqui, H. et al. (2020). Salinity induced physiological and biochemical changes in plants. An omic approach towards salt stress tolerance. Plant Physiol. Biochem., 156, 64–77. https://doi.org/10.1016/j.plaphy.2020.08.042 DOI: https://doi.org/10.1016/j.plaphy.2020.08.042
  13. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol., 24(1), 1. DOI: https://doi.org/10.1104/pp.24.1.1
  14. Ashraf, M., Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Sci., 166(1), 3–16. https://doi.org/10.1016/j.plantsci.2003.10.024 DOI: https://doi.org/10.1016/j.plantsci.2003.10.024
  15. Ashraf, M.A., Ashraf, M., Ali, Q. (2010). Response of two genetically diverse wheat cultivars to salt stress at different growth stages: leaf lipid peroxidation and phenolic contents. Pak. J. Bot., 42(1), 559–565.
  16. Askary, M., Talebi, S.M., Amini, F. et al. (2017). Effects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija, 63(1). https://doi.org/10.6001/biologija.v63i1.3476 DOI: https://doi.org/10.6001/biologija.v63i1.3476
  17. Azarmi-Atajan, F., Sayyari-Zohan, M.H. (2020). Alleviation of salt stress in lettuce (Lactuca sativa L.) by plant growth-promoting rhizobacteria. J. Hortic. Postharvest Res., 3(Spec. Issue), 67–78.
  18. Babaei, K., Seyed Sharifi, R., Pirzad, A. et al. (2017). Effects of bio fertilizer and nano Zn-Fe oxide on physiological traits, antioxidant enzymes activity and yield of wheat (Triticum aestivum L.) under salinity stress. J. Plant Interact., 12(1), 381–389. https://doi.org/10.1080/17429145.2017.1371798 DOI: https://doi.org/10.1080/17429145.2017.1371798
  19. Bacakova, L., Pajorova, J., Tomkova, M. et al. (2020). Applications of nanocellulose/nanocarbon composites: focus on biotechnology and medicine. Nanomaterials, 10(2), 196. https://www.mdpi.com/2079-4991/10/2/196 DOI: https://doi.org/10.3390/nano10020196
  20. Barbieri, G., Vallone, S., Orsini, F. et al. (2012). Stomatal density and metabolic determinants mediate salt stress adaptation and water use efficiency in basil (Ocimum basilicum L.). J. Plant Physiol., 169(17), 1737–1746. https://doi.org/10.1016/j.jplph.2012.07.001 DOI: https://doi.org/10.1016/j.jplph.2012.07.001
  21. Bi, X., Fang, Y., Zhang, S. et al. (2025). Plant-nanoparticle interactions with g-C3N4: regulator development, metabolic responses, and physiological mechanisms. ACS Appl. Mater. Interfaces., 17(46), 63322–63333. https://doi.org/10.1021/acsami.5c13408 DOI: https://doi.org/10.1021/acsami.5c13408
  22. Bistgani, Z.E., Hashemi, M., DaCosta, M. et al. (2019). Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Ind. Crops. Prod., 135, 311–320. https://doi.org/10.1016/j.indcrop.2019.04.055 DOI: https://doi.org/10.1016/j.indcrop.2019.04.055
  23. Bor, J.-Y., Chen, H.-Y., Yen, G.-C. (2006). Evaluation of Antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables. J. Agric. Food Chem., 54(5), 1680–1686. https://doi.org/10.1021/jf0527448 DOI: https://doi.org/10.1021/jf0527448
  24. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3 DOI: https://doi.org/10.1016/0003-2697(76)90527-3
  25. Cai, L., Wei, X., Feng, H. et al. (2021). Antimicrobial mechanisms of g-C3N4 nanosheets against the oomycetes Phytophthora capsici: disrupting metabolism and membrane structures and inhibiting vegetative and reproductive growth. J. Hazard. Mater., 417, 126121. https://doi.org/10.1016/j.jhazmat.2021.126121 DOI: https://doi.org/10.1016/j.jhazmat.2021.126121
  26. Cao, W., Bi, S., Chi, C. et al. (2022). Effects of high salinity stress on the survival, gill tissue, enzyme activity and free amino acid content in razor clam Sinonovacula constricta. Front. Mar. Sci., 9. https://doi.org/10.3389/fmars.2022.839614 DOI: https://doi.org/10.3389/fmars.2022.839614
  27. Chang, C.-C., Yang, M.-H., Wen, H.-M. et al. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 10(3). DOI: https://doi.org/10.38212/2224-6614.2748
  28. Chen, L., Song, J. (2017). Tailored graphitic carbon nitride nanostructures: synthesis, modification, and sensing applications. Adv. Funct. Mater., 27(39), 1702695. https://doi.org/10.1002/adfm.201702695 DOI: https://doi.org/10.1002/adfm.201702695
  29. Chen, X., Tian, X., Shin, I. et al. (2011). Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species. Chem. Soc. Rev., 40(9), 4783–4804. https://doi.org/10.1039/C1CS15037E DOI: https://doi.org/10.1039/c1cs15037e
  30. Chung, I.-M., Rajakumar, G., Subramanian, U. et al. (2019). Impact of copper oxide nanoparticles on enhancement of bioactive compounds using cell suspension cultures of Gymnema sylvestre (Retz.) R. Br. Appl. Sci., 9(10), 2165. https://doi.org/10.3390/app9102165 DOI: https://doi.org/10.3390/app9102165
  31. Demo, A.H., Gemeda, M.K., Abdo, D.R. et al. (2025). Impact of soil salinity, sodicity, and irrigation water salinity on crop production and coping mechanism in areas of dryland farming. Agrosys. Geosci. Environ, 8(1), e70072. https://doi.org/10.1002/agg2.70072 DOI: https://doi.org/10.1002/agg2.70072
  32. EFSA, Sci. Comm., More, S., Bampidis, V. et al. (2021). Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health. EFSA J., 19(8), e06768. https://doi.org/10.2903/j.efsa.2021.6768 DOI: https://doi.org/10.2903/j.efsa.2021.6768
  33. Elemike, E.E., Uzoh, I.M., Onwudiwe, D.C. et al. (2019). The role of nanotechnology in the fortification of plant nutrients and improvement of crop production. Appl. Sci., 9(3), 499. https://doi.org/10.3390/app9030499 DOI: https://doi.org/10.3390/app9030499
  34. FAO (2023). FAOStat. Available: https://www.fao.org/faostat/en/#data/QCL [date of access: 14.03.2025].
  35. Feng, Y., Kreslavski, V.D., Shmarev, A.N. et al. (2022). Effects of iron oxide nanoparticles (Fe3O4) on growth, photosynthesis, antioxidant activity and distribution of mineral elements in wheat (Triticum aestivum) plants. Plants, 11(14), 1894. https://doi.org/10.3390/plants11141894 DOI: https://doi.org/10.3390/plants11141894
  36. Fernández, V., Del Río, V., Pumariño, L. et al. (2008). Foliar fertilization of peach (Prunus persica (L.) Batsch) with different iron formulations: effects on re-greening, iron concentration and mineral composition in treated and untreated leaf surfaces. Sci. Hortic., 117(3), 241–248. https://doi.org/10.1016/j.scienta.2008.05.002 DOI: https://doi.org/10.1016/j.scienta.2008.05.002
  37. Foyer, C.H., Hanke, G. (2022). ROS production and signalling in chloroplasts: cornerstones and evolving concepts. Plant J., 111(3), 642–661. https://doi.org/10.1111/tpj.15856 DOI: https://doi.org/10.1111/tpj.15856
  38. Ghadakchi asl, A., Mozafari, A.a., Ghaderi, N. (2019). Iron nanoparticles and potassium silicate interaction effect on salt-stressed grape cuttings under in vitro conditions: a morphophysiological and biochemical evaluation. In Vitro Cell. Dev. Biol., 55(5), 510–518. https://doi.org/10.1007/s11627-019-09988-0 DOI: https://doi.org/10.1007/s11627-019-09988-0
  39. Gharsallah, C., Fakhfakh, H., Grubb, D. et al. (2016). Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars. AoB Plants, 8, plw055. https://doi.org/10.1093/aobpla/plw055 DOI: https://doi.org/10.1093/aobpla/plw055
  40. Gholami, R., Fahadi Hoveizeh, N., Zahedi, S.M. et al. (2024). Nanostructure-assisted drought tolerance in olive trees (Olea europaea L.): the role of Fe2O3-graphitic carbon. Front. Plant Sci., 15. https://doi.org/10.3389/fpls.2024.1454619 DOI: https://doi.org/10.3389/fpls.2024.1454619
  41. Ghosh, D., Adhikary, A., Roy, S. (2025). Synthesis of Fe-loaded mesoporous silica nanoparticles for improving the growth, ion homeostasis, osmolyte, and oxidative status of wheat seedlings under salinity stress. New J. Chem., 49(12), 4958–4981. https://doi.org/10.1039/D4NJ05538A DOI: https://doi.org/10.1039/D4NJ05538A
  42. González-García, Y., López-Vargas, E.R., Cadenas-Pliego, G. et al. (2019). Impact of carbon nanomaterials on the antioxidant system of tomato seedlings. Int. J. Mol. Sci., 20(23), 5858. https://doi.org/10.3390/ijms20235858 DOI: https://doi.org/10.3390/ijms20235858
  43. Guo, Z., Chen, Q., Liang, T. et al. (2023). Functionalized carbon nano-enabled plant ROS signal engineering for growth / defense balance. Nano Today, 53, 102045. https://doi.org/10.1016/j.nantod.2023.102045 DOI: https://doi.org/10.1016/j.nantod.2023.102045
  44. Habibi, G., Sarvary, S. (2015). The roles of selenium in protecting lemon balm against salt stress. Iran. J. Plant Physiol., 5(1), 1425–1433.
  45. Hajiboland, R., Norouzi, F., Poschenrieder, C. (2014). Growth, physiological, biochemical and ionic responses of pistachio seedlings to mild and high salinity. Trees, 28(4), 1065–1078. https://doi.org/10.1007/s00468-014-1018-x DOI: https://doi.org/10.1007/s00468-014-1018-x
  46. Hasanuzzaman, M., Bhuyan, M.H.M.B., Zulfiqar, F. et al. (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants, 9(8), 681. https://doi.org/10.3390/antiox9080681 DOI: https://doi.org/10.3390/antiox9080681
  47. Hassanpouraghdam, M.B., Mehrabani, L.V., Khoshmaram, L. et al. (2025). Amelioration of the growth and physiological responses of Capsicum annum L. via quantum dot-graphene oxide, cerium oxide, and titanium oxide nanoparticles foliar application under salinity stress. Sci. Rep., 15(1), 467. https://doi.org/10.1038/s41598-024-84706-4 DOI: https://doi.org/10.1038/s41598-024-84706-4
  48. Hassanpouraghdam, M.B., Mehrabani, L.V., Tzortzakis, N. (2020). Foliar application of nano-zinc and iron affects physiological attributes of Rosmarinus officinalis and quietens NaCl salinity depression. J. Soil Sci. Plant Nutr., 20(2), 335–345. https://doi.org/10.1007/s42729-019-00111-1 DOI: https://doi.org/10.1007/s42729-019-00111-1
  49. Heath, R. L., Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125(1), 189–198. https://doi.org/10.1016/0003-9861(68)90654-1 DOI: https://doi.org/10.1016/0003-9861(68)90654-1
  50. Heidarpour, H., Padervand, M., Soltanieh, M., Vossoughi, M. (2020). Enhanced decolorization of rhodamine B solution through simultaneous photocatalysis and persulfate activation over Fe/C3N4 photocatalyst. Chem. Eng. Res. Des., 153, 709–720. https://doi.org/10.1016/j.cherd.2019.09.007 DOI: https://doi.org/10.1016/j.cherd.2019.09.007
  51. Inagaki, M., Tsumura, T., Kinumoto, T. et al. (2019). Graphitic carbon nitrides (g-C3N4) with comparative discussion to carbon materials. Carbon, 141, 580–607. https://doi.org/10.1016/j.carbon.2018.09.082 DOI: https://doi.org/10.1016/j.carbon.2018.09.082
  52. Jamil, M., Rehman, S., Rha, E. (2007). Salinity effect on plant growth, PSII photochemistry and chlorophyll content in sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea capitata L.). Pak. J. Bot., 39(3), 753–760.
  53. Jamshidi Goharrizi, K., Baghizadeh, A., Kalantar, M. et al. (2020). Combined effects of salinity and drought on physiological and biochemical characteristics of pistachio rootstocks. Sci. Hortic., 261, 108970. https://doi.org/10.1016/j.scienta.2019.108970 DOI: https://doi.org/10.1016/j.scienta.2019.108970
  54. Kaliyaperumal, R., Nagaraj, K., Kasilingam, T. et al. (2025). Synthesis, characterization, and biological evaluation of PbO/g-C₃N₄ nanocomposite: a multifunctional material for antimicrobial, antioxidant, anti-inflammatory, and cytotoxic applications. BioNanoSci., 15(3), 451. https://doi.org/10.1007/s12668-025-02082-3 DOI: https://doi.org/10.1007/s12668-025-02082-3
  55. Kamran, M., Parveen, A., Ahmar, S. et al. (2019). An overview of hazardous impacts of soil salinity in crops, tolerance mechanisms, and amelioration through selenium supplementation. Int. J. Mol. Sci., 21(1), 148. DOI: https://doi.org/10.3390/ijms21010148
  56. Karimi, H.R., Sadeghi-Seresht, E. (2018). Effects of salinity stress on growth indices, physiological parameters and element concentration in Banebaghi (Pistacia sp.) as rootstock for pistachio. J. Plant Nutr., 41(9), 1094–1103. https://doi.org/10.1080/01904167.2017.1392572 DOI: https://doi.org/10.1080/01904167.2017.1392572
  57. Karpinska, B., Foyer, C.H. (2024). Superoxide signalling and antioxidant processing in the plant nucleus. J. Exp. Bot., 75(15), 4599–4610. https://doi.org/10.1093/jxb/erae090 DOI: https://doi.org/10.1093/jxb/erae090
  58. Kaya, C., Ashraf, M. (2024). Foliar fertilization: a potential strategy for improving plant salt tolerance. Crit. Rev. Plant Sci., 43(2), 94–115. https://doi.org/10.1080/07352689.2023.2270253 DOI: https://doi.org/10.1080/07352689.2023.2270253
  59. Khan, M.N., Mobin, M., Abbas, Z.K. et al. (2017). Role of nanomaterials in plants under challenging environments. Plant Physiol. Biochem., 110, 194–209. https://doi.org/10.1016/j.plaphy.2016.05.038 DOI: https://doi.org/10.1016/j.plaphy.2016.05.038
  60. Khanizadeh, P., Mumivand, H., Morshedloo, M.R. et al. (2024). Application of Fe2O3 nanoparticles improves the growth, antioxidant power, flavonoid content, and essential oil yield and composition of Dracocephalum kotschyi Boiss. Front. Plant Sci., 15. https://doi.org/10.3389/fpls.2024.1475284 DOI: https://doi.org/10.3389/fpls.2024.1475284
  61. Kiełkowska, A. (2017). Cytogenetic effect of prolonged in vitro exposure of Allium cepa L. root meristem cells to salt stress. Cytol. Genet., 51(6), 478–484. https://doi.org/10.3103/S0095452717060068 DOI: https://doi.org/10.3103/S0095452717060068
  62. Kiełkowska, A., Grzebelus, E., Lis-Krzyścin, A. et al. (2019). Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity. Plant Cell Tiss. Organ Cult., 137(2), 379–395. https://doi.org/10.1007/s11240-019-01578-7 DOI: https://doi.org/10.1007/s11240-019-01578-7
  63. Kokina, I., Plaksenkova, I., Jermaļonoka, M. et al. (2020). Impact of iron oxide nanoparticles on yellow medick (Medicago falcata L.) plants. J. Plant Interact., 15(1), 1–7. https://doi.org/10.1080/17429145.2019.1708489 DOI: https://doi.org/10.1080/17429145.2019.1708489
  64. Kumar, S., Li, G., Yang, J. et al. (2020). Investigation of an antioxidative system for salinity tolerance in Oenanthe javanica. Antioxidants, 9(10), 940. https://doi.org/10.3390/antiox9100940 DOI: https://doi.org/10.3390/antiox9100940
  65. Kwon, J., Mao, X., Lee, J. (2017). Fe-based multifunctional nanoparticles with various physicochemical properties. Curr. Appl. Phys., 17(8), 1066–1078. https://doi.org/10.1016/j.cap.2017.04.018 DOI: https://doi.org/10.1016/j.cap.2017.04.018
  66. Lalitha, G.R., Meena, S., Sridevi, G. et al. (2025). Boosting sustainable agriculture: how nanofertilisers tackle abiotic stress. Commun. Soil Sci. Plant Anal., 56(14), 2220–2247. https://doi.org/10.1080/00103624.2025.2489793 DOI: https://doi.org/10.1080/00103624.2025.2489793
  67. Li, P., Xia, Y., Song, K. et al. (2024). The impact of nanomaterials on photosynthesis and antioxidant mechanisms in gramineae plants: research progress and future prospects. Plants, 13(7), 984. https://doi.org/10.3390/plants13070984 DOI: https://doi.org/10.3390/plants13070984
  68. Li, X., Yang, T., He, S. et al. (2025). Sustainable remediation of tetracycline-contaminated soil: a Fe-g-C₃N₄ nanozyme driven abiotic-biotic strategy. 1–32. http://dx.doi.org/10.2139/ssrn.5588794 DOI: https://doi.org/10.2139/ssrn.5588794
  69. Ma, C., Borgatta, J., Hudson, B.G. et al. (2020). Advanced material modulation of nutritional and phytohormone status alleviates damage from soybean sudden death syndrome. Nat. Nanotechnol., 15(12), 1033–1042. https://doi.org/10.1038/s41565-020-00776-1 DOI: https://doi.org/10.1038/s41565-020-00776-1
  70. Mendis, C.L., Padmathilake, R.E., Attanayake, R.N. et al. (2025). Learning from Salicornia: physiological, biochemical, and molecular mechanisms of salinity tolerance. Int. J. Mol. Sci., 26(13), 5936. https://doi.org/10.3390/ijms26135936 DOI: https://doi.org/10.3390/ijms26135936
  71. Moradbeygi, H., Jamei, R., Heidari, R. et al. (2020). Investigating the enzymatic and non-enzymatic antioxidant defense by applying iron oxide nanoparticles in Dracocephalum moldavica L. plant under salinity stress. Sci. Hortic., 272, 109537. https://doi.org/10.1016/j.scienta.2020.109537 DOI: https://doi.org/10.1016/j.scienta.2020.109537
  72. Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environ., 25(2), 239–250. https://doi.org/10.1046/j.0016-8025.2001.00808.x DOI: https://doi.org/10.1046/j.0016-8025.2001.00808.x
  73. Munns, R., Tester, M. (2008). Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 59, 651–681. https://doi.org/10.1146/annurev.arplant.59.032607.092911 DOI: https://doi.org/10.1146/annurev.arplant.59.032607.092911
  74. Nakano, Y., Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22(5), 867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232 DOI: https://doi.org/10.1093/oxfordjournals.pcp.a076232
  75. Nourozi, E., Hosseini, B., Maleki, R. et al. (2019). Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy-root cultures. J. Sci. Food Agric., 99(14), 6418–6430. https://doi.org/10.1002/jsfa.9921 DOI: https://doi.org/10.1002/jsfa.9921
  76. OECD (2020). Guidance document on the safety testing of manufactured nanomaterials. OECD Series on the Safety of Manufactured Nanomaterials No. 85. Organisation for Economic Co‑operation and Development, Paris. https://www.oecd.org/en/topics/nanomaterials-and-advanced-materials.html [date of access: 11.03.2025].
  77. Ong, W.-J., Tan, L.-L., Ng, Y.H. et al. (2016). Graphitic carbon nitride (g-C3N4)-based photocatalysts for artificial photosynthesis and environmental remediation: are we a step closer to achieving sustainability? Chem. Rev., 116(12), 7159–7329. https://doi.org/10.1021/acs.chemrev.6b00075 DOI: https://doi.org/10.1021/acs.chemrev.6b00075
  78. Pain, D., Dancis, A. (2016). Roles of Fe–S proteins: from cofactor synthesis to iron homeostasis to protein synthesis. Curr. Opin. Genet. Dev., 38, 45–51. https://doi.org/10.1016/j.gde.2016.03.006 DOI: https://doi.org/10.1016/j.gde.2016.03.006
  79. Pushnik, J.C., Miller, G.W., Manwaring, J.H. (1984). The role of iron in higher plant chlorophyll biosynthesis, maintenance and chloroplast biogenesis. J. Plant Nutr., 7(1–5), 733–758. https://doi.org/10.1080/01904168409363238 DOI: https://doi.org/10.1080/01904168409363238
  80. Rahneshan, Z., Nasibi, F., Lakehal, A. et al. (2018). Unravelling salt stress responses in two pistachio (Pistacia vera L.) genotypes. Acta Physiol. Plant., 40(9), 172. https://doi.org/10.1007/s11738-018-2745-1 DOI: https://doi.org/10.1007/s11738-018-2745-1
  81. Rahneshan, Z., Nasibi, F., Moghadam, A.A. (2018). Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistacia vera L.) rootstocks. J. Plant Interact., 13(1), 73–82. https://doi.org/10.1080/17429145.2018.1424355 DOI: https://doi.org/10.1080/17429145.2018.1424355
  82. Rao, S., Shekhawat, G.S. (2016). Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea. 3 Biotech, 6(2), 244. https://doi.org/10.1007/s13205-016-0550-3 DOI: https://doi.org/10.1007/s13205-016-0550-3
  83. Rasouli, F., Asadi, M., Hassanpouraghdam, M.B. et al. (2022). Foliar application of ZnO-NPs influences chlorophyll fluorescence and antioxidants pool in Capsicum annum L. under Salinity. Horticulturae, 8(10), 908. https://doi.org/10.3390/horticulturae8100908 DOI: https://doi.org/10.3390/horticulturae8100908
  84. Rico, C.M., Peralta-Videa, J.R., Gardea-Torresdey, J.L. (2015). Chemistry, biochemistry of nanoparticles, and their role in antioxidant defense system in plants. In: M.H. Siddiqui, M.H. Al-Whaibi, F. Mohammad (eds.), Nanotechnology and plant sciences: nanoparticles and their impact on plants. Springer International Publishing, 1–17. https://doi.org/10.1007/978-3-319-14502-0_1 DOI: https://doi.org/10.1007/978-3-319-14502-0_1
  85. Rui, M., Ma, C., Hao, Y. et al. (2016). Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea). Front. Plant Sci., 7, 815. https://doi.org/10.3389/fpls.2016.00815 DOI: https://doi.org/10.3389/fpls.2016.00815
  86. Saed-Moucheshi, A., Shekoofa, A., Pessarakli, M. (2014). Reactive oxygen species (ROS) generation and detoxifying in plants. J. Plant Nutr., 37(10), 1573–1585. https://doi.org/10.1080/01904167.2013.868483 DOI: https://doi.org/10.1080/01904167.2013.868483
  87. Sakihama, Y., Yamasaki, H. (2002). Lipid peroxidation induced by phenolics in conjunction with aluminum ions. Biol. Plant., 45(2), 249–254. https://doi.org/10.1023/A:1015152908241 DOI: https://doi.org/10.1023/A:1015152908241
  88. Sairam, R. K., Shukla, D. S., & Saxena, D. C. (1997). Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biol. Plant., 40(3), 357-364. https://doi.org/10.1023/A:1001009812864 DOI: https://doi.org/10.1023/A:1001009812864
  89. Saleem, A., Zulfiqar, A., Ali, B. et al. (2022). Iron sulfate (FeSO4) improved physiological attributes and antioxidant capacity by reducing oxidative stress of Oryza sativa L. cultivars in alkaline soil. Sustainability, 14(24), 16845. https://doi.org/10.3390/su142416845 DOI: https://doi.org/10.3390/su142416845
  90. Samuolienė, G., Brazaitytė, A., Viršilė, A. et al. (2019). Nutrient levels in Brassicaceae microgreens increase under tailored light-emitting diode spectra. Front. Plant Sci., 10. https://doi.org/10.3389/fpls.2019.01475 DOI: https://doi.org/10.3389/fpls.2019.01475
  91. Shafi, Z., Shahid, M., Ilyas, T. et al. (2025). Unveiling the plant growth regulators crosstalk in agricultural crop response to salinity-stress: a concise review. Physiol. Plant., 177(4), e70402. https://doi.org/10.1111/ppl.70402 DOI: https://doi.org/10.1111/ppl.70402
  92. Shahid, M.A., Sarkhosh, A., Khan, N. et al. (2020). Insights into the physiological and biochemical impacts of salt stress on plant growth and development. Agronomy, 10(7), 938. https://doi.org/10.3390/agronomy10070938 DOI: https://doi.org/10.3390/agronomy10070938
  93. Sim, C., Zaharah, A., Tan, M. et al. (2015). Rapid determination of leaf chlorophyll concentration, photosynthetic activity and NK concentration of Elaies guineensis via correlated SPAD-502 chlorophyll index. Asian J. Agric. Res., 9, 132–138. https://doi.org/10.3923/ajar.2015.132.138 DOI: https://doi.org/10.3923/ajar.2015.132.138
  94. Singh, A., Agrawal, S., Rajput, V.D. et al. (2024). Nanoparticles in revolutionizing crop production and agriculture to address salinity stress challenges for a sustainable future. Discov. Appl. Sci., 6(6), 317. https://doi.org/10.1007/s42452-024-06009-7 DOI: https://doi.org/10.1007/s42452-024-06009-7
  95. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16(3), 144–158. DOI: https://doi.org/10.5344/ajev.1965.16.3.144
  96. Sinha, S., Saxena, R., Singh, S. (2005). Chromium induced lipid peroxidation in the plants of Pistia stratiotes L.: role of antioxidants and antioxidant enzymes. Chemosphere, 58(5), 595–604. https://doi.org/10.1016/j.chemosphere.2004.08.071 DOI: https://doi.org/10.1016/j.chemosphere.2004.08.071
  97. Tiwari, A., Mamedov, F., Grieco, M. et al. (2016). Photodamage of iron–sulphur clusters in photosystem I induces non-photochemical energy dissipation. Nat. Plants., 2(4), 16035. https://doi.org/10.1038/nplants.2016.35 DOI: https://doi.org/10.1038/nplants.2016.35
  98. Torabian, S., Zahedi, M., Khoshgoftar, A.H. (2017). Effects of foliar spray of nano-particles of FeSO4 on the growth and ion content of sunflower under saline condition. J. Plant Nutr., 40(5), 615–623. https://doi.org/10.1080/01904167.2016.1240187 DOI: https://doi.org/10.1080/01904167.2016.1240187
  99. Tsai, Y.-C., Chen, K.-C., Cheng, T.-S. et al. (2019). Chlorophyll fluorescence analysis in diverse rice varieties reveals the positive correlation between the seedlings salt tolerance and photosynthetic efficiency. BMC Plant Biol., 19(1), 403. https://doi.org/10.1186/s12870-019-1983-8 DOI: https://doi.org/10.1186/s12870-019-1983-8
  100. Verma, K.K., Song, X.-P., Joshi, A. et al. (2022). Recent trends in nano-fertilizers for sustainable agriculture under climate change for global food security. Nanomaterials, 12(1), 173. https://doi.org/10.3390/nano12010173 DOI: https://doi.org/10.3390/nano12010173
  101. Wang, H., Zhang, M., Song, Y. et al. (2018). Carbon dots promote the growth and photosynthesis of mung bean sprouts. Carbon, 136, 94–102. https://doi.org/10.1016/j.carbon.2018.04.051 DOI: https://doi.org/10.1016/j.carbon.2018.04.051
  102. Yin, R., Messner, B., Faus-Kessler, T. et al. (2012). Feedback inhibition of the general phenylpropanoid and flavonol biosynthetic pathways upon a compromised flavonol-3-O-glycosylation. J. Exp. Bot., 63(7), 2465–2478. https://doi.org/10.1093/jxb/err416 DOI: https://doi.org/10.1093/jxb/err416
  103. Yoshimura, K., Yabuta, Y., Ishikawa, T. et al. (2000). Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiol., 123(1), 223–234. https://doi.org/10.1104/pp.123.1.223 DOI: https://doi.org/10.1104/pp.123.1.223
  104. Zhang, Y., Li, L., Dai, H. et al. (2025). Iron oxide nanoparticles (FeO-NPs) mitigate salt stress in peanut seedlings by enhancing photosynthesis, osmoregulation, and antioxidant activity. Plant Physiol. Biochem., 227, 110206. https://doi.org/10.1016/j.plaphy.2025.110206 DOI: https://doi.org/10.1016/j.plaphy.2025.110206
  105. Zhou, Y., Feng, C., Wang, Y. et al. (2024). Understanding of plant salt tolerance mechanisms and application to molecular breeding. Int. J. Mol. Sci., 25(20), 10940. https://doi.org/10.3390/ijms252010940 DOI: https://doi.org/10.3390/ijms252010940
  106. Zhuang, Z., Yu, Y., Dong, S. et al. (2024). Carbon-based nanozymes: design, catalytic mechanisms, and environmental applications. Anal. Bioanal. Chem. Res., 416(27), 5949–5964. https://doi.org/10.1007/s00216-024-05405-7 DOI: https://doi.org/10.1007/s00216-024-05405-7

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