CHANGES IN THE CONCENTRATIONS OF PHENOLIC ACIDS IN CARROT PLANTS INOCULATED WITH Alternaria radicina Meier, Drechsler & Eddy
Bożena Cwalina-AmbroziakUniversity of Warmia and Mazury in Olsztyn
Ryszard AmarowiczPolish Academy of Sciences in Olsztyn
Małgorzata GłosekUniversity of Warmia and Mazury in Olsztyn
Michał JaniakPolish Academy of Sciences in Olsztyn
Similary to other fungal species of the genus Alternaria, Alternaria radicina is a major pathogen that infects both the aboveground and underground parts of carrot
plants. Changes in the concentrations of phenolic compounds are observed in infected carrot plants. Carrot seedlings were inoculated with the most pathogenic isolates of Alternaria radicina selected in a laboratory test. A phytopathological analysis was performed to determine the health status of carrot plants. The concentrations of phenolic acids in petioles were determined four weeks after inoculation with A. radicina and at harvest. The results of a greenhouse experiment revealed more severe disease symptoms on carrot plants cv. Koral inoculated with A. radicina isolates, compared with cv. Bolero. The content of the predominant phenolic acid (chlorogenic acid) was found to decrease in the leaf stalks of carrots cv. Koral inoculated with A. radicina. A statistically non-significant increase
in chlorogenic acid levels was noted in the leaf stalks of carrots cv. Bolero in the experimental and control groups.
Keywords:Daucus carota L., plant health, pathogens, phenolic compounds
Babik J., Kaniszewski S., Babik I. P., 2011. The usefulness of vegetable species and cultivars for organic cultivation. J. Res. Appl. Agric. Engin. 56, 15–21.
Barkai-Golan R., 2001. Postharvest diseases of fruits and vegetables. Development and control. Elsevier, Amsterdam, The Netherlands, 418 pp.
Brandt K., Molgaard J.P., 2001. Organic agriculture: does it enhance or reduce the nutritional value of plant foods? J. Sci. Food Agric. 81, 924–931.
Ellis M.B., 1971. Dematiaceus hyphomycetes. Commonwealth Mycological Institute Kew Surrey, England.
Feucht W., Treutter D., Polster J., 2004. Flavanol binding of nuclei from tree species. Plant Cell Rep. 22, 430–436.
Freytag S., Arabatzis N., Hahlbrock K., Schmelzer E., 1994. Reversible cytoplasmic arrangements precede wall apposition, hypersensitive cell death and defense-related gene activation in potato Phytophthora infestans interactions. Planta 194, 123–135.
Gleń K., 2008. Evaluation of foliar fertilizers for horseradish (Armoracia rusticana Gaernt.) against fungal diseases. Ecol. Chem. Engineer. 15, 331–336.
Grzebelus D., Barański R., Reby E., 2003. A laboratory test petiole assay of carrot susceptibility to Alternaria radicina. Fol. Hort. 15(2), 41–48.
Gugino B.K., Caroll J.E, Widmer T.L,Chen P., Abawi G.S., 2007. Field evaluation of carrot cultivars for susceptibility to fungal leaf blight diseases in New York. Crop Prot. 26, 709–714.
Hallmann E., Sikora M., Rembiałkowska E., Marszałek K., Lipowski J., 2011. The influence of pasteurization process on nutritive value of carrot juices from organic and conventional production. J. Res. Appl. Agric. Engin. 56, 133–137.
Irzykowska L., Karolewski L., Weber Z., 2007. Porównanie występowania grzybów na materiale siewnym i na roślinach marchwi pochodzących z tego materiału. Prog. Plant Prot./Post. Ochr. Rośl. 47(2), 109–113.
Lafuente M.T., Cantwell M., Yang S.T., Rubatzky V., 1989. Isocoumarin content of carrot as influenced by ethylene concentration, storage temperature and stress conditions. Acta Horticult. 258, 523–534.
Pawelec A., Dubourg C., Briard M., 2006. Evaluation of carrot resistance to Alternaria leaf blight in controlled environments. Plant Pathol. 55, 68–72.
Pryor B.M., 2002. Black rot. In: Compendium of umbelliferous crops diseases, Davis R.M., Raid R.N. (ed.). APS Press, The American Phytopathological Society: St. Paul, Minnesota, 25–27.
Smoleń S., Sady W., 2007. The effect of foliar nutrition with urea, molybdenum, sucrose and benzyladenine on yield and some organic compounds of carrot storage roots. Rocz. AR Pozn. 383 Ogrodn. 41, 619–623.
Souza R.T., Forcelini C.A., Reis M.E., Calvete E.O., 2001. Frequency of Alternaria dauci and Cercospora carotae as causal agents of carrot leaf blights in Passo Fundo, RS. Fitopat. Brasil. 26, 614–618.
Stoll T., Schweiggert U., Schieber A., Carle R., 2003. Application of hydrolyzed carrot pomace as a functional food ingredient to beverages. Food Agric. Environ. 1, 88–92.
Szafrańska K., Kowalska U., Górecka K., Amarowicz R., Urbalewicz A., Górecki R., Janas K.M., 2010. Influence of copper ions on the plant material obtained from the anther culture of carrot (Daucus carota L.). Veg. Crops Res. Bull. 73, 23–31.
Tarko T., Duda-Chodak A., Bebak A., 2012. Biological activity of selected fruit and vegetable pomaces. Żywność, Nauka, Technologie, Jakość 4, 55–65.
Weidner S., Brosowska-Arendt W., Szczechura W., Karamać M., Kosińska A., Amarowicz R., 2011. Effect of osmotic stress and post-stress recovery on the content of phenolics and properties of antioxidants in germinating seeds of grapevine Vitis californica. Acta Soc. Bot. Pol. 80, 11–19.
Weidner S., Karolak M., Karamać M., Kosińska A., Amarowicz R., 2009. Phenolic compounds and properties of antioxidants in grapevine roots (Vitis vinifera L.) under drought stress followed by recovery. Acta Soc. Bot. Pol. 78, 97–103.
Wróbel M., Karamać M., Amarowicz R., Frączek E., Weidner S., 2005. Metabolism of phenolic compounds in Vitis riparia seeds during stratification and during germination under optimal and low temperature stress conditions. Acta Physiol. Plant. 27, 313–320.
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.