In vitro ESTIMATE OF INFLUENCE OF Silphium perfoliatum L. LEAVES EXTRACT ON SOME FUNGI COLONIZING THE PEPPER PLANTS
Agnieszka Jamiołkowska
University of Life Sciences in LublinRadosław Kowalski
University of Life Sciences in LublinAbstract
Biological control is a modern, comprehensive and non-polluting approach to the management of diseases. Control of plant pathogen by using biological preparations derived from plants like garlic, mint, thyme, grapefruit, has shown attractive and promising results. Present study aimed at laboratory evaluation the properties of ethanol extract of Silphium perfoliatum leaves towards fungi colonizing pepper plants grown in the field. The mycelium of Alternaria alternata, Botrytis cinerea, Colletotrichum coccodes, Fusarium oxysporum, Penicillium expansum, Trichoderma harzianum were used in experiment. The Silphium extract was applied in two concentrations: 5% and 10%. Leaves were obtained from the three-year S. perfoliatum plantation. Tested fungal isolates originated from pepper plants grown in the field. The studies made use of a Petri dishes method recommended for testing fungicides in laboratory conditions. The Silphium extracts in two
tested concentrations significantly inhibited the growth of tested fungi species, with the exception of T. harzianum and B. cinerea in 5% concentration extract. Effects of 10% extract were longer than those of 5% one. A. alternata and C. coccodes were fungi, growth of which was the most strongly inhibited by tested concentrations of Silphium extracts.
Keywords:
antifungal activity, A. alternata, C. coccodes, biocontrolReferences
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Bohlmann F., Jakupovic J., 1980. Neue Sesquiterpen-Kohlenwasserstoffe mit anomalen Kohlenstoffgerüst aus Silphium-Arten. Phytochemistry 19, 259–265.
Calabria L.M., Piacente S., Kapusta I., Dharmawardhane S.F., Segarra F.M., Pessiki P.J., Mabry T.J., 2008. Triterpene saponins from Silphium radula. Phytochemistry 69, 961–972.
Copping L.G., 1996. Crop protection agents from nature: Natural products and analogues. The Royal Society of Chemistry, London, pp. 501.
Davidjanc E.S., Abubakirov N.K., 1992. Chemical composition and prospects of utilizing plants of the genus Silphium L. Rast. Resursy 28, 118–128.
Davidjanc E.S., Kartaševa I.A., Nešin I.W., 1997. The effect of triterpene glycosides of Silphium perfoliatum L. on phytopathogenic fungi. Rast. Resursy 4, 93–98.
El-Sayed N.H., Wojcińska M., Drost-Karbowska K., Matławska I., Williams J., Mabry T.J., 2002.
Kaempferol triosides from Silphium perfoliatum. Phytochemistry 60, 835–838.
Jamiołkowska A., 2009a. The influence of bio-preparation Biosept 33 SL on fungi colonizing of sweet pepper plants (Capsicum annuum L.) cultivated in the field. EJPAU, Horticulture, 12, 3, www.ejpau.media.pl
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Jamiołkowska A., 2011. Laboratory effect of azoxystrobin (Amistar 250SC) and grapefruit extract (Biosept 33SL) on growth of fungi colonizing zucchini plants. Acta Sci. Pol., Hortorum Cultus 10(2), 245–257.
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Kowalski R., 2005. Analysis of lipophilic fraction from leaves, inflorescences and rhizomes of Silphium perfoliatum L. Acta Soc. Bot. Pol. 74, 5–10.
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Kowalski R., 2007b. Studies of selected plant raw materials as alternative sources of triterpenes of oleanolic and ursolic acid types. J. Agr. Food Chem. 55, 656–662.
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Kowalski R., 2008d. Antimicrobial activity of essential oils and extracts of rosinweed (Silphium trifoliatum and Silphium integrifolium) – plants used by the American Indians. Flav. Fragr. J. 23, 426–433.
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Kowalski R., Wolski T., 2001. Characteristic of growth and development of Silphium perfoliatum L. in the first years of cutilivation. Annales UMCS, sec. EEE Hoticultura, 9 supl. 311–317.
Kowalski R, Wolski T., 2003. TLC and HPLC analysis of the phenolic acids in Silphium perfoliatum L. leaves, inflorescences and rhizomes. J. Planar. Chromatogr. 16, 230–236.
Kowalski R., Wolski T., 2005. The chemical composition of essential oils of Silphium perfoliatum L. Flav. Fragr. J. 20, 306–310.
Kujanceva A.M., Davidjanc E.S., 1988. Silphium perfoliatum extract’s regeneration activity. Farmacija Moskwa 6, 36–37.
Lahlou M., 2004. Methods to study the phytochemistry and bioactivity of essential oils. Phytotherapy Res. 18, 435–448.
Lingk W., 1991. Heath risk evaluation of pesticide contamination in drinking water. Gesunde Pflanzen 43, 21–25.
Mesbah L.A., Van der Weerden G.M., Nijkamp H.J.J., Hille J., 2000. Sensitivity among species of Solanaceae to AAL toxins produced by Alternaria altrnata f. sp. lycopersici. Plant Pathology 49(6), 734–741.
Nelson P.E., Toussoun T.A., Marasas W.F.O., 1983. Fusarium species. An Illustrated Manual for Identification. The Pensylvania State University Park and London, pp.193.
NIST/EPA/NIH. 2002. Mass Spectral Library. USA.
Papavizas G.S., 1985. Trichoderma and Gliocladium. Biology, ecology and potential for biocontrol. Ann. Rev. of Phytopath. 23, 23–54.
Pcolinski M.J., Doskotch R.W., Lee A.Y., Clardy J., 1994. Chlorosilphanol A and silphanepoxol, labdane diterpenes from Silphium perfoliatum. J. Nat. Prod. 57, 776–783.
Pięta D., Patkowska E., 2003. The role of antagonistic fungi and bacteria limiting the occurrence of some phytopathogens inhabiting the soybean soil environment. EJPAU, Horticulture, 6, 2, www.ejpau.media.pl.
Polish Pharmacopoeia VI. 2002. Polskie Towarzystwo Farmaceutyczne, Warszawa. p. 150–151.
Rotem J., 1994. The genus Alternaria: Biology, Epidemiology and Pathogenicity. APS Press, St. Paul, pp. 326.
Singelton V.L., Rossi J.A. Jr., 1965. Colorimetry of total phenolics with phosphomlybdic – phosphotungstic acid reagents. Amer. J. Enol. Viticult. 16, 144–158.
Suarez-Estrella F., Vargas-Garcia C., Lopez M.J., Capel C., Moreno J., 2007. Antagonistic activity of bacteria and fungi from horticultural compost against Fusarium oxysporum f. sp. melonis. Crop Protection 26(1), 46–53.
Syrov W.N., Chušbaktova Z.A., Davidjanc E.S., 1992. The triterpene glicosides of Silphium perfoliatum L. Hipolipidemic activity of silphiozyd. Chim. Farm. Żurnal 26, 66–69.
Thanassoulopoulos C.C., Giannopolitis C.N., Vitsos G.T., 1971. Evaluation of sensitiveness and development of resistance of Fusarium oxysporum f. sp. lycopersici to benomyl. Phytopathol. Zeitsch. 70, 114–120.
Van den Dool H., Kratz D.J., 1963. A generalization the retention index system including liner temperature programmed gas-liquid partion chromatography. J. Chromatogr. 11, 463–467.
Wagner A., 2004. Fungal communities colonizing stems of hot pepper (Capsicum annuum L.). Phytopathol. Pol. 33, 23–29.
Ahmed A.S., Sanchez C., Candela E.M., 2000. Evaluation of induction of systemic resistance in pepper plants (Capsicum annuum) of Phytophtora capsici using Trichoderma harzianum and its relation with capsidiol accumulation. Eur. J. Plan Pathol. 106(9), 817–824.
Bohlmann F., Jakupovic J., 1979. Neue labdan-derivate und sesquiterpene aus Silphium- arten. Phytochemistry 18, 1987–1992.
Bohlmann F., Jakupovic J., 1980. Neue Sesquiterpen-Kohlenwasserstoffe mit anomalen Kohlenstoffgerüst aus Silphium-Arten. Phytochemistry 19, 259–265.
Calabria L.M., Piacente S., Kapusta I., Dharmawardhane S.F., Segarra F.M., Pessiki P.J., Mabry T.J., 2008. Triterpene saponins from Silphium radula. Phytochemistry 69, 961–972.
Copping L.G., 1996. Crop protection agents from nature: Natural products and analogues. The Royal Society of Chemistry, London, pp. 501.
Davidjanc E.S., Abubakirov N.K., 1992. Chemical composition and prospects of utilizing plants of the genus Silphium L. Rast. Resursy 28, 118–128.
Davidjanc E.S., Kartaševa I.A., Nešin I.W., 1997. The effect of triterpene glycosides of Silphium perfoliatum L. on phytopathogenic fungi. Rast. Resursy 4, 93–98.
El-Sayed N.H., Wojcińska M., Drost-Karbowska K., Matławska I., Williams J., Mabry T.J., 2002.
Kaempferol triosides from Silphium perfoliatum. Phytochemistry 60, 835–838.
Jamiołkowska A., 2009a. The influence of bio-preparation Biosept 33 SL on fungi colonizing of sweet pepper plants (Capsicum annuum L.) cultivated in the field. EJPAU, Horticulture, 12, 3, www.ejpau.media.pl
Jamiołkowska A., 2009b. Fungi isolated from underground part of hot pepper (Capsicum annuum) plants cultivated in the field. Phytopathol. Pol. 51, 37–44.
Jamiołkowska A., 2011. Laboratory effect of azoxystrobin (Amistar 250SC) and grapefruit extract (Biosept 33SL) on growth of fungi colonizing zucchini plants. Acta Sci. Pol., Hortorum Cultus 10(2), 245–257.
Jamiołkowska A., Buczkowska H., 2009. Grzyby występujące na papryce słodkiej (Capsicum annuum L.) w uprawie polowej. Progr. Plant Prot. /Post. Ochr. Rośl.49 (1), 201–208.
Joulain, D., König, W.A., 1998. The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. E. B. – Verlag, Hamburg, Germany.
Kalinowska M., Zimowski J., Pączkowski C., Wojciechowski, Z.A., 2005. The formation of sugar chains in triterpenoid saponins and glycoalkaloids. Phytochemistry Rev. 4, 237–257.
Kowalski R., 2004. Secondary metabolites in Silphium integrifolium in the first 2 years of cultivation. New. Zeal. J. Crop. Hort. 32, 397–406.
Kowalski R., 2005. Analysis of lipophilic fraction from leaves, inflorescences and rhizomes of Silphium perfoliatum L. Acta Soc. Bot. Pol. 74, 5–10.
Kowalski R., 2007a. Silphium trifoliatum L. – a new alternative cultivation herbal plant? Acta. Agr. Scand. B – S. P. 57 (2), 155–156.
Kowalski R., 2007b. Studies of selected plant raw materials as alternative sources of triterpenes of oleanolic and ursolic acid types. J. Agr. Food Chem. 55, 656–662.
Kowalski R., 2008a. The chemical composition of essential oils and lipophilic extracts of Silphium integrifolium Michx. and Silphium trifoliatum L. leaves. Flav. Fragr. J. 23, 164–171.
Kowalski R., 2008b. The chemical composition of essential oils and lipophilic extracts of Silphium integrifolium Michx. and Silphium trifoliatum L. inflorescences. Chem. Natur. Compounds 44 (2), 241–244.
Kowalski R., 2008c. The chemical composition of essential oils and lipophilic extracts of Silphium integrifolium Michx. and Silphium trifoliatum L. rhizomes. J. Essential Oil Res. 20, 255–259.
Kowalski R., 2008d. Antimicrobial activity of essential oils and extracts of rosinweed (Silphium trifoliatum and Silphium integrifolium) – plants used by the American Indians. Flav. Fragr. J. 23, 426–433.
Kowalski R., Kędzia B., 2007. Evaluation of antibacterial activity of Silphium perfoliatum extracts. Pharm. Biol. 6, 494–500.
Kowalski R., Wierciński J., Mardarowicz M., 2005. Essential oil in leaves and inflorescences of Silphium integrifolium Michx. J. Essent. Oil Res. 17, 220–222.
Kowalski R., Wolski T., 2001. Characteristic of growth and development of Silphium perfoliatum L. in the first years of cutilivation. Annales UMCS, sec. EEE Hoticultura, 9 supl. 311–317.
Kowalski R, Wolski T., 2003. TLC and HPLC analysis of the phenolic acids in Silphium perfoliatum L. leaves, inflorescences and rhizomes. J. Planar. Chromatogr. 16, 230–236.
Kowalski R., Wolski T., 2005. The chemical composition of essential oils of Silphium perfoliatum L. Flav. Fragr. J. 20, 306–310.
Kujanceva A.M., Davidjanc E.S., 1988. Silphium perfoliatum extract’s regeneration activity. Farmacija Moskwa 6, 36–37.
Lahlou M., 2004. Methods to study the phytochemistry and bioactivity of essential oils. Phytotherapy Res. 18, 435–448.
Lingk W., 1991. Heath risk evaluation of pesticide contamination in drinking water. Gesunde Pflanzen 43, 21–25.
Mesbah L.A., Van der Weerden G.M., Nijkamp H.J.J., Hille J., 2000. Sensitivity among species of Solanaceae to AAL toxins produced by Alternaria altrnata f. sp. lycopersici. Plant Pathology 49(6), 734–741.
Nelson P.E., Toussoun T.A., Marasas W.F.O., 1983. Fusarium species. An Illustrated Manual for Identification. The Pensylvania State University Park and London, pp.193.
NIST/EPA/NIH. 2002. Mass Spectral Library. USA.
Papavizas G.S., 1985. Trichoderma and Gliocladium. Biology, ecology and potential for biocontrol. Ann. Rev. of Phytopath. 23, 23–54.
Pcolinski M.J., Doskotch R.W., Lee A.Y., Clardy J., 1994. Chlorosilphanol A and silphanepoxol, labdane diterpenes from Silphium perfoliatum. J. Nat. Prod. 57, 776–783.
Pięta D., Patkowska E., 2003. The role of antagonistic fungi and bacteria limiting the occurrence of some phytopathogens inhabiting the soybean soil environment. EJPAU, Horticulture, 6, 2, www.ejpau.media.pl.
Polish Pharmacopoeia VI. 2002. Polskie Towarzystwo Farmaceutyczne, Warszawa. p. 150–151.
Rotem J., 1994. The genus Alternaria: Biology, Epidemiology and Pathogenicity. APS Press, St. Paul, pp. 326.
Singelton V.L., Rossi J.A. Jr., 1965. Colorimetry of total phenolics with phosphomlybdic – phosphotungstic acid reagents. Amer. J. Enol. Viticult. 16, 144–158.
Suarez-Estrella F., Vargas-Garcia C., Lopez M.J., Capel C., Moreno J., 2007. Antagonistic activity of bacteria and fungi from horticultural compost against Fusarium oxysporum f. sp. melonis. Crop Protection 26(1), 46–53.
Syrov W.N., Chušbaktova Z.A., Davidjanc E.S., 1992. The triterpene glicosides of Silphium perfoliatum L. Hipolipidemic activity of silphiozyd. Chim. Farm. Żurnal 26, 66–69.
Thanassoulopoulos C.C., Giannopolitis C.N., Vitsos G.T., 1971. Evaluation of sensitiveness and development of resistance of Fusarium oxysporum f. sp. lycopersici to benomyl. Phytopathol. Zeitsch. 70, 114–120.
Van den Dool H., Kratz D.J., 1963. A generalization the retention index system including liner temperature programmed gas-liquid partion chromatography. J. Chromatogr. 11, 463–467.
Wagner A., 2004. Fungal communities colonizing stems of hot pepper (Capsicum annuum L.). Phytopathol. Pol. 33, 23–29.
Agnieszka Jamiołkowska
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Radosław Kowalski
University of Life Sciences in Lublin
University of Life Sciences in Lublin
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