The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
Danuta Kozak
University of Life Sciences in Lublin, PolandMarzena Parzymies
University of Life Sciences in Lublin, PolandAlicja Świstowska
University of Life Sciences in Lublin, PolandBarbara Marcinek
University of Life Sciences in Lublin, PolandBairam Solomon Ismael
University of Tikrit, IraqAbstract
Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
Keywords:
regeneration, multiplication rate, explant defoliation, explant decapitationReferences
Biondo, R., Soares, A.M., Bertoni, B.W., Franca, S.C., Pereira, A.M. S. (2004). Direct organogenesis of Mandevilla illustris (Vell.) Woodson and effect of its aquaeous extract on the enzymatic and toxic activities of Crotalus durissus terrificus snake venom. Plant Cell Rep., 22, 549–552.
Biondo, R., Souza, A.V., Bertoni, B.W., Soares, A.M., Franca, S.C., Pereira, A.M.S. (2007). Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson. Sci. Agric. (Piracicaba, Braz.), 64(3), 263–268.
Bermejo, C., Espósito, M.A., Cravero, V., Anido, F.L., Cointry, E. (2012). In vitro plant regeneration from cotyledonary nodes of recombinant inbred lines of lentil. Sci. Hortic., 134, 13–19.
Cordeiro, S.Z., Simas, N.K., Henriques, A.B., Lage, C.L.S., Sato, A. (2012). Micropropagation of Mandevilla moricandiana (A.D.C.) Woodson. In Vitro Cell. Dev. Biol. Plant, 48, 620–626.
Cordeiro, S.Z., Simas, N.K., Henriques, A.B., Sato, A. (2014). Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil. Crop Breed. Appl. Biotechnol., 14(2). http://dx.doi.org/10.1590/1984-70332014v14n2a19
Debnath, S.C. (2005). Micropropagation of lingonberry: influence of genotype, explant orientation, and overcoming TDZ-induced inhibition of shoot elongation using zeatin. HortScience, 40(1), 185–188.
Handro, W., Floh, E.I.S., Ferreira, C.M., Guerra, M.P. (1988). Tissue, cell culture and micropropagation of Mandevilla velutina, a natural source of bradykinin antagonist. Plant Cell Rep., 7, 564–566.
Kalimuthu, K., Prabakaran, R. (2014). In vitro Micropropagation of Syngonium podophyllum. Int. J. Pure App. Biosci. 2(4), 88–92.
Kozak, D. (1991). Shoots regeneration from various parts of Narcissus cv. Carlton through tissue culture. Prace Inst. Sad. Kwiac., B Rośl. Ozdobne, 16, 41–48.
Kozak, D., Pogroszewska, E., Szmagara, M. (2013). The influence of type and orientation of explants on in vitro growth and development of Cosmos atrosanguineus (Hook.) Voss. Acta Sci. Pol. Hortorum Cultus, 12(1), 41–53.
Kucharska, D., Golis, M., Podwyszyńska, M., Wiśniewska-
-Grzeszkiewicz, H., Orlikowska, T. (2000). Propagation of Rosa manetti rootstock in vitro. Zesz. Nauk. Inst. Sad. Kwiac., 7, 365–374.
Miller, R.M., Drew, R.A. (1990). Effect of explant type on proliferation of Carica papaya L. in vitro. Plant Cell. Tiss. Organ Cult. 21, 39–44.
Mohamed-Yasseen, Y. (2002). Micropropagation of pitaya (Hylocereus undatus Britton et Rose). In Vitro Cell. Dev. Biol. Plant., 38(5), 427–429.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.
Ngamau, K. (2001). Development of an in vitro culture procedure using seeds from Zantedeschia aethiopica ‘Green Goddess’ as explant. Gartenbauwissenschaft, 66(3), 133–139.
Nobre, J. (1994). In vitro shoot proliferation of Myrtus communis L. from field-grown plants. Sci. Hortic. 58(3), 253–258.
Orlikowska, T., Sabała, I., Kucharska, D. (2000). The effect of leaf and shoot tip removal and explant orientation on axillary shoot proliferation of Codiaeum variegatum Blume var. pictum Muell. Arg. cv. Excellent. Sci. Hortic., 85, 103–111.
Pumisutapon, P., Visser, R.G.F., De Klerk, G.-J. (2009). Apical dominance in Alstroemeria cultured in vitro. Acta Hortic. 829, 145–148.
Rajeswari, V., Paliwal, K. (2008). In vitro adventitious shoot regeneration from seedling explant of Albizia odoratissima L.f. (Benth.). In Vitro Cell. Dev. Biol. Plant. 44(2), 78–83.
Renau-Morata, B., Ollero, J., Arrilaga, I., Segura, J. (2005). Factors influencing axillary shoot proliferation and adventitious budding in cedar. Tree Physiol., 25(4), 477–486.
Saini, R., Jaiwal, P.K. (2002). Age, position in mother seedling, orientation, and polarity of the epicotyl segments of blackgram (Vigna mungo L. Hepper) determines its morphogenic response. Plant Sci., 163(1), 101–109.
Saini, H.K., Gill, M.S., Gill, M.I.S. (2010). Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lush.). Indian J. Biotechnol., 9, 419–423.
Seabrook, J.E.A., Cumming, B.G., Dionne, L.A. (1976). The in vitro induction of adventitious shoot and root apices on Narcissus (daffodil and narcissus) cultivar tissue. Can. J. Bot., 54, 814–819.
Shimizu-Sato, S., Tanaka, M., Mori, H. (2009). Auxin–cytokinin interactions in the control of shoot branching. Plant Mol. Biol., 69, 429–435.
Voyiatzi, Ch., Voyiatzis, D.G., Tsiakmaki, V. (1995). In vitro shoot proliferation rates of the rose cv. (hybrid tea) ‘Dr. Verhage’, as affected by apical dominance regulating substances. Sci. Hortic. 61, 241–249.
Wojtania, A., Gabryszewska, E., Woźniak, P. (2006). Zastosowanie kultur in vitro w rozmnażaniu Dipladenia sanderi Hemsl. Zesz. Probl. Postęp. Nauk Rol., 510, 717–722.
Yonghong, L., Daxing, Z., Lijuan, X. (2004). Studies of the micropropagation of Mandevilla sanderi. For. Sci. Technol., 29(5), 49–51.
Ziv, M., Halevy, A.H., Shilo, R., 1970. Organ and plantlets regeneration of Gladiolus through tissue culture. Ann. Bot., 34, 671–675.
University of Life Sciences in Lublin, Poland
University of Life Sciences in Lublin, Poland
University of Life Sciences in Lublin, Poland
University of Life Sciences in Lublin, Poland
University of Tikrit, Iraq
License
Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.
The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.
