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ASPHC Baner

Vol. 23 No. 6 (2024)

Articles

Influence of agrotechnical and varietal factors on biodiversity of fungi colonizing amaranth seeds

DOI: https://doi.org/10.24326/asphc.2024.5429
Submitted: September 10, 2024
Published: 2024-12-20

Abstract

Amaranth seeds are characterized by a very high nutritional value, which depends on various environmental factors and variety. For this plant, it is crucial to maintain the appropriate post-harvest quality of the seeds, particularly in terms of fungal infections. The aim of this study was to evaluate the impact of increasing NPK doses, row spacing, and variety on the biodiversity of fungi colonizing amaranth seeds. For this purpose, a 3-year field experiment (2016–2018) was carried out involving the cultivation of two varieties – ‘Rawa’ and ‘Aztek’ – under the soil and climatic conditions of southeastern Poland. The experiment demonstrated that most of the analyzed research factors (NPK fertilization, row spacing, and variety) had a significant impact on the health status of amaranth seeds. Increasing NPK fertilization led to higher seed colonization by Alternaria alternata. Row spacing affected both the total number of fungal colonies and the population of Aspergillus brasiliensis on the seeds. In this regard, amaranth cultivation was more favorable with wide row spacing than narrow row spacing. The variety significantly influenced seed germination after harvest, and the total number of fungal colonies on the seeds, particularly of Aspergillus brasiliensis and Alternaria alternata. The variety ‘Rawa’ was characterized by lower seed germination capacity and was more frequently populated by pathogenic fungi, which negatively affected seed quality compared to the variety ‘Aztek’. Considering the production of seeds free of post-harvest biological contamination in southeastern Poland, it is advisable to cultivate the variety ‘Aztek’ in a wide row spacing system.

References

  1. Akinnibosun, F.I., Adeola, M.O. (2015). Quality assessment and proximate analysis of Amaranthus hybridus, Celo-sia argentea and Talinum triangulare obtained from open markets in Benin City, Nigeria. J. Appl. Sci. Environ. Manag., 19(4), 727–734. https://doi.org/10.4314/jasem.v19i4.21
  2. Allemann, J., Denner, F.D.N. (2006). Allelopathic influences of soil planted to Amaranthus cruentus L. on two toma-to (Lycopersicon esculentum Mill.) cultivars. S. Afr. J. Plant Soil, 23(2), 142–143. https://doi.org/10.1080/02571862.2006.10634745
  3. Amanullah, J., Shahzad, A., Inamullah, A., Musharaf, A. (2014). Influence of sowing time and nitrogen fertilization on Alternaria leaf blight and oil yield of sesame cultivars. Pure Appl. Biol., 3(4), 160–166. https://doi.org/10.19045/bspab.2014.34005
  4. Aslam, A., Naz, F., Arshad, M., Qureshi, R., Rauf, C.A. (2010). In vitro antifungal activity of selected medicinal plant diffusates against Alternaria solani, Rhizoctonia solani and Macrophomina phaseolina. Pak. J. Bot., 42(4), 2911–2919.
  5. Bakker, M.G., Brown, D.W., Kelly, A.C., Kim, H.S., Kurtzman, C.P., Mc Cormick, S.P., O’Donnell, K.L., Proctor, R.H., Vaughan, M.M., Ward, T.J. (2018). Fusarium mycotoxins: a trans-disciplinary overview. Canad. J. Plant Pathol., 40(2), 161–171. https://doi.org/10.1080/07060661.2018.1433720
  6. Blodgett, J.T., Swart, W.J. (2002). Infection, colonization, and disease of Amaranthus hybridus leaves by the Alter-naria tenuissima Group. Plant Dis. 86, 1199–1205. Available: https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2002.86.11.1199 [date of access: 20.07.2024].
  7. Blodgett, J.T., Swart, W.J., Louw, S.V. (2004). Identification of fungi and fungal pathogens associated with Hypo-lixus haerens and decayed and cankered stems of Amaranthus hybridus. Plant Dis., 88(4), 333–337. https://doi.org/10.1094/PDIS.2004.88.4.333
  8. Booth, C. (1971). The genus Fusarium. CMI, Kew, Surrey, pp. 236.
  9. Cabañes, F.J., Bragulat, M.R., Castellá, G. (2010). Ochratoxin A producing species in the genus Penicillium. Toxins, 10(2), 1111–1120. https://doi.org/10.3390/toxins2051111
  10. Chen, Y.Q., Cheng, J.H., Sun, D.W. (2020). Chemical, physical and physiological quality attributes of fruit and veg-etables induced by cold plasma treatment: Mechanisms and application advances. Crit. Rev. Food Sci. Nutr., 60(16), 2676–2690. https://doi.org/10.1080/10408398.2019.1654429
  11. Chen, W.Q., Swart, W.J. (2001). Genetic variation among Fusarium oxysporum isolates associated with root rot of Amaranthus hybridus in South Africa. Plant Dis., 85(10), 1076–1080. https://doi.org/10.1094/pdis.2001.85.10.1076
  12. Chen, W.Q., Swart, W.J. (2002). The in vitro phytotoxicity of culture filtrates of Fusarium oxysporum to five geno-types of Amaranthus hybridus. Euphytica, 127, 61–67. https://doi.org/10.1023/A%3A1019980600219
  13. Ellis, J.P. (1977). The genera Trichothyrina and Actinopeltis in Britain. Transact. Brit. Mycol. Soc., 68(2), 145–155.
  14. Escrivá, L., Oueslati, S., Font, G., Manyes, L. (2017). Alternaria mycotoxins in food and feed: An overview. Hindawi J. Food Qual., 2017(1), 1569748. https://doi.org/10.1155/2017/1569748
  15. Fierro-Cruz, J.E., Jiménez, P., Coy-Barrera, E. (2017). Fungal endophytes isolated from Protium heptaphyllum and Trattinnickia rhoifolia as antagonists of Fusarium oxysporum. Rev. Argentina Microbiol., 49(3), 255–263. https://doi.org/10.1016/j.ram.2016.12.009
  16. Fojutowski, A., Kropacz, A. (2015). Growth of Chaetomium globosum fungus in laboratory conditions on agar me-dium and Scots pine wood. Ann. Warsaw Univ. Life Sci. SGGW Forestry Wood Technol., 92, 97–103. https://doi.org/10.20883/issn.2353-9798
  17. Hamim, I., Mohanto, D.C., Sarker, M.A., Ali, M.A. (2014). Effect of seed borne pathogens on germination of some vegetable seeds. J. Phytopathol. Pest Manag., 1(1), 34–51.
  18. Hassani, F., Zare, L., Khaledi, M. (2019). Evaluation of germination and vigor indices associated with Fusarium-infected seeds in pre-basic seeds wheat fields. J. Plant Protect. Res., 59(1), 69–85. https://doi.org/10.24425/jppr.2019.126037
  19. He, M-H., Wang, Y-P., Wu, E-J., Shen, L-L., Yang, L-N., Wang, T., Shang, L-P., Zhu, W., Zhan, J. (2019). Constrain-ing evolution of Alternaria alternata resistance to a demethylation inhibitor (DMI) fungicide difenoconazole. Front. Microbiol., 10, 1–12. https://doi.org/10.3389/fmicb.2019.01609
  20. Irzykowska, L., Kononowicz, M. (2017). Ocena zdrowotności nasion oraz uzyskiwanych z nich kiełków przezna-czonych do konsumpcji. Prog. Plant Prot., 57(4), 250–254. https://doi.org/10.14199/ppp-2017-039
  21. Jamiołkowska, A., Skwaryło-Bednarz, B., Patkowska, E., Buczkowska, H., Gałązka, A., Grządziel, J., Kopacki, M. (2020). Effect of mycorrhizal inoculation and irrigation on biological properties of sweet pepper rhizosphere in organic field cultivation. Agronomy, 10(11), 1693. https://doi.org/10.3390/agronomy10111693
  22. Jensen, B., Knudsen, I.M.B., Madsen, M., Jensen, D.F. (2004). Biopriming of infected carrot seed with an antago-nist, Clonostachys rosea, selected for control of seedborne Alternaria spp. Phytopathology, 94, 551–560. https://doi.org/10.1094/PHYTO.2004.94.6.551
  23. Jensen, B.D., Knorr, K., Nicolaisen, M. (2016). In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains. Eur. J. Plant Pathol., 146, 657–670. https://doi.org/10.1007/s10658-016-0950-6
  24. Klich, M.A. (2007). Aspergillus flavus: the major producer of aflatoxin. Mol. Plant Pathol., 8(6), 713–722. https://doi.org/10.1111/J.1364-3703.2007.00436.X
  25. Kopacki, M., Wagner, A., Michałek, W. (2016). Pathogenicity of Fusarium oxysporum, Fusarium avenaceum and Sclerotinia sclerotiorum and its effect on photosynthetic activity of chrysanthemum plants. Acta Sci. Pol. Hor-torum Cultus, 15(3), 59–70.
  26. Kopacki, M., Pawłat, J.,Skwaryło-Bednarz, B., Jamiołkowska, A., Stępniak, P.M., Kiczorowski, P., Golan, K. (2021). Physical crop postharvest storage and protection methods. Agronomy, 11, 93. https://doi.org/10.3390/agronomy11010093
  27. Krasowska, P. (2022). Effect of different NPK fertilisation and cultivar on the size, quality and healthiness of ama-ranth seed yield. Ph.D. thesis. Typescript, University of Life Sciences in Lublin, Poland.
  28. Lamichhane, J.R., You, M.P., Laudinot, V., Barbetti, M.J., Aubertot, J-N. (2020). Revisiting sustainability of fungi-cide seed treatments for field crops. Plant Dis., 104(3), 610–623. https://doi.org/10.1094/PDIS-06-19-1157-FE
  29. Marcinkowska, J. (2010). Oznaczanie rodzajów ważnych organizmów fitopatogenicznych (Fungi, Oomycota, Plasmodiophorida) [Identification of important phytopathogenic organisms (Fungi, Oomycota, Plasmodiophor-ida)]. Wyd. SGGW, Warszawa, 216 pp.
  30. Marcinkowska, J. (2012). Oznaczanie rodzajów grzybów sensu lato ważnych w fitopatologii [Identification of fungi sensu lato important in phytopathology]. Wyd. PWRiL Warszawa, pp. 508.
  31. Masternak, H., Kulikowska, A. (2010). Co niszczy ogród? [What destroys a garden?]. Oficyna Wydawnicza Multico Warszawa, 12–60.
  32. Matyjaszczyk, E. (2011). Obowiązek wprowadzenia integrowanej ochrony roślin i związane z nim szanse dla rol-nictwa ekologicznego [Obligatory integrated pest management and related opportunities for organic farming]. J. Res. Appl. Agric. Eng., 56(4), 37–40.
  33. Mielniczuk, E., Skwaryło-Bednarz, B. (2020). Fusarium Head Blight, mycotoxins and strategies for their reduction. Agronomy, 10(4), 509. https://doi.org/10.3390/agronomy10040509
  34. Narkiewicz-Jodko, M. (1986). Wartość siewna przechowywanego ziarna trzech zbóż w aspekcie fitopatologicznym [Sowing value of stored seeds of three cereal crops-phytopathological aspect]. Zesz. Nauk. AR Wroc. Rozpr., 55.
  35. Narkiewicz-Jodko, M. (1998). Zdrowotność ziarna zbóż jako wskaźnik jego jakości [Grain health as an indicator of its quality]. Zesz. Nauk. AR Wroc. 328, Technol. Żywn., 12, 85–93.
  36. Narkiewicz-Jodko, M., Gil, Z. (1997). Wpływ przedplonu na zdrowotność i jakość pszenicy ozimej [The impact of preceding crops on the health and quality of winter wheat]. Plant Breed. Seed Sci., 41(1), 83–88.
  37. Nelson, P.E., Toussoun, T.A., Marasas W.F.O. (1983). Fusarium species: An illustrated manual DOR identification. The Pennsylvania State University Press, 193 pp.
  38. Ninganagouda, S., Rathod, V., Singh, D. (2014). Characterization and biosynthesis of silver nanoparticles using the fungus Aspergillus niger. Int. Lett. Nat. Sci., 15, 49–57. https://doi.org/10.56431/p-hwnei8
  39. Noelting, M.C., Sisterna, M., Lovisolo, M., Molla-Kralj, A., Lori G., Sandoval, M.C., Sulyok, M., Molina, M.C. (2016). Discoloured seeds of amaranth plant infected by Alternaria alternata: physiological, histopathological alterations and fungal secondary metabolites associated or registered. J. Plant Prot. Res., 56(3), 244–249. https://doi.org/10.1515/jppr-2016-0036
  40. Nunuparov, M., Khotemlyansky, P., Panchishin, V., Krasnoshekov, V., Valiullin, L., Kislov, A., Konishchev, M. (2019). Wireless system for remote monitoring of temperature and humidity in the grain storage and grain dryer. IOP Conf. Series, Earth and Environmental Science, 390. https://doi.org/10.1088/1755-1315/390/1/012034
  41. Oteiza, J.M., Khaneghah, A.M., Campagnollo, F.B., Granato, D., Mahmoudi, M.R., Sant’Ana, A.S., Gianuzzi, L. (2017). Influence of production on the presence of patulin and ochratoxin A in fruit juices and wines of Argen-tina. LWT – Food Science and Technology, 80, 200–2007. https://doi.org/10.1016/j.lwt.2017.02.025
  42. Oufensou, S., Dessì, A., Dallocchio, R., Balmas, V., Azara, E., Carta, P., Migheli, Q., Delogu, G. (2021). Molecular Docking and Comparative Inhibitory Efficacy of Naturally Occurring Compounds on Vegetative Growth and Deoxynivalenol Biosynthesis in Fusarium culmorum. Toxins, 13(11), 759. https://doi.org/10.3390/toxins13110759
  43. Oyeyiola, G.P. (2002). Fungi present in the root zone of Amaranthus hybridus. Bioscience Research Communica-tions, 14(3). Available: https://ojs.klobexjournals.com/index.php/brj/article/view/878/931 [date of access: 10.07.2024].
  44. Perincherry, L., Lalak-Kańczugowska, J., Stępień, Ł. (2019). Fusarium-produced mycotoxins in plant-pathogen interactions. Toxins, 11(11), 664. https://doi.org/10.3390/toxins11110664
  45. Polska Norma, PN-79R-65950. Materiał siewny. Metody badania nasion [Seed material. Methods for seed testing].
  46. Próchniak, J. (2005). Podstawy rolnictwa ekologicznego [Fundamentals of organic farming]. Wyd. LODR, Końskowola, 5.
  47. Pusz, W. (2008). Occurrence of dark spots on stems of Amaranthus species. Phytopathologia Polonica, 48, 53–57.
  48. Pusz, W. (2009a). Fungi from seeds of Amaranthus spp. Phytopathologia, 54, 15–21.
  49. Pusz, W. (2009b). Morpho-physiological and molecular analyses of Alternaria alternata isolated from seeds of Amaranthus. Phytopathologia, 54, 5–14.
  50. Pusz, W. (2009c). Ocena zdrowotności liści roślin z rodzaju Amaranthus [Assessment of leaf health in Amaranthus plants]. Zesz. Nauk. UP Wrocław, ser. Roln., 95, 574, 50–60.
  51. Pusz, W., Pląskowska, E. (2012). Fungi inhabiting Amaranthus paniculatus L. Available: https://www.researchgate.net/profile/Wojciech-Pusz/publication/263419517_Fungi_inhabiting_Amaranthus_paniculatus/links/57127c8d08aeff315ba0d568/Fungi-inhabiting-Amaranthus-paniculatus.pdf [date of access: 2.08.2024].
  52. Pusz, W., Pląskowska, E., Yildirim, I., Weber, R. (2015). Fungi occurring on the plants of the genus Amaranthus L. Turk. J. Bot., 39(1), 147–161. https://doi.org/10.3906/bot-1403-106
  53. Raper, K., Thom, C., Fennel, D. (1949). A manual of the Penicillium. The Williams and Wilkins Co., Baltimore.
  54. Sadowski, Cz., Lenc, L., Wyczling, D. (2007). Grzyby z rodzaju Fusarium występujące w ziarnie wybranych od-mian pszenicy uprawianej w rejonie Żuław [Fusarium fungi present in the grain of selected wheat varieties cul-tivated in the Żuławy Region]. Prog. Plant Prot./Post. Ochr. Roślin, 47(2), 306–309.
  55. Saharan, G.S., Mehta, N., Meena, P.D. (2016). Alternaria diseases of crucifers: biology, ecology and disease man-agement. Springer, Singapore, Heidelberg, New York, Dordrecht, London, 299 pp. https://doi.org/10.1007/978-981-10-0021-8
  56. Sałata, B., Rudnicka-Jezierska, W. (1979). Grzyby (Mycota) [Fungi (Mycota)]. Vol. 12, Wyd. PWN Warszawa, Kra-ków, 216 pp.
  57. Sankar, R., Ghosh, T.K., Ray, H., Ghosh, A., Saha, S., Ghosh, D., Bhattacharyya, N. (2020). Detection of optimum fumigation in grain storage using FUMON in: Joshi A., Mahmud M., Ragel R.G., Thakur N.V. (eds.), Information and communication technology for competitive strategies (ICTCS 2020). Lecture Notes in Networks and Sys-tems 191. https://doi.org/10.1007/978-981-16-0739-4_90
  58. Skwaryło-Bednarz, B., Stępniak, P., Jamiołkowska, A., Kopacki, M., Krzepiłko, A., Klikocka, H. (2020). Amaranth seeds as a source of nutrients and bioactive substances in human diet. Acta Sci. Pol., Hortorum Cultus, 19(6), 153–164. https://doi.org/10.24326/asphc.2020.6.13
  59. Szot, B. (1999). Właściwości agrofizyczne amarantusa (Amaranthus cruentus) [Agrophysical properties of ama-ranth (Amaranthus cruentus)]. Acta Agrophys., 18, 1–73.
  60. Yassin, M.A., El-Samawaty, A.E.M.A., Dawoud, T.M., Abd-Elkader, O.H., Al Maary, K.S., Hatamleh, A.A., El-gorban, A.M. (2016). Characterization and anti-Aspergillus flavus impact of nanoparticles synthesized by Pen-icillium citrinum. Saudi J. Biol. Sci., 24(6), 1243–1248. https://doi.org/10.1016/j.sjbs.2016.10.004

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