CHARACTERISTICS OF Valdensia heterodoxa Peyr. AS AN Ericaceae PATHOGEN IN POLAND

Wojciech Kukuła

Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland

Ewa Mirzwa-Mróz

Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland

Wojciech Wakuliński

Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland

Elżbieta Paduch-Cichal

Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland



Abstract

Valdensia heterodoxa as a parasitic fungus was observed on Ericaceae family plants i.e. blueberry (Vaccinium corymbosum L.), bilberry (V. myrtillus L.) and lingonberry (V. vitis-idaea L.). Isolates of this polyphagous were obtained from the infected leaves of different cultivars of highbush blueberry collected from commercial plantation in Mazovia province and from bilberry collected from forests in Pomerania province. PCR amplification of selected rDNA fragments (ITS1, 5.8S, ITS2) was done with ITS1F and ITS4A primers. Bioinformatic analysis revealed similarity 99–100% between selected nucleotide sequences of V. heterodoxa isolates from bilberry and highbush blueberry. The sequences of bilberry isolates were obtained and described for the first time in Poland. Their reference sequence was deposited in GenBank (KT121733). In laboratory experiments conidia of selected bilberry isolates on OA medium were 278 ±6 × 140 ±4 μm. Conidia from highbush blueberry, bilberry, and lingonberry were measured. Depending on the host plant conidia were different in the length of the arms and width of the head. The growth of the fungal isolates on PDA (potato dextrose agar), OA (oatmeal agar), WOA (weak oatmeal agar), SNA (salt nutrient agar) media was examined. The cultures were divided into two groups based on their morphology on PDA medium.

Keywords:

Vaccinium corymbosum, valdensia leaf blight, bilberry, PCR identification

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Published
2017-08-31



Wojciech Kukuła 
Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland
Ewa Mirzwa-Mróz 
Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland
Wojciech Wakuliński 
Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland
Elżbieta Paduch-Cichal 
Department of Plant Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Warsaw, Poland



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