BIOLOGICAL CONTROL OF ROOT ROT DISEASE CAUSED BY Rhizoctonia solani Kühn ON POTATO AND BEAN USING ANTAGONIST BACTERIA
Mesude Figen Donmez
Igdir University Igdir, TurkeyBadel Uysal
Igdir University Igdir, TurkeyErkol Demırcı
Karadeniz Technical University, Maçka Vocational School, Trabzon, TurkeySezai Ercisli
Atatürk University, Erzurum, TurkeyRamazan Cakmakcı
Atatürk University, Erzurum, TurkeyAbstract
In this study the use of 73 bacteria, isolated from rhizosphere area of tea (Camellia sinensis) plants in Black Sea Region in Turkey, as potential biocontrol agent against root rot disease caused by Rhizoctonia solani that was known as an important disease on potato and bean plants in Turkey and in the world were investigated. In in vitro conditions, 73 bacteria used for antagonistic tests, and 15 out of 74 were found to be effective against R. solani and formed between 0.20–2.30 cm inhibition zone and inhibited mycelium development of the pathogen. In vivo pot experiment also showed that antago-nist bacteria strains had various level inhibition effect (between 12% and 83%) on R. solani compared to control. The study revealed that biocontrol agents might play an essential role in management of root rot diseases in potato and bean.
Keywords:
bean, potato, Rhizoctonia solani, biological controlReferences
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Townsend, G.K., Heuberger, J.W. (1943). Methods for estimating losses caused by diseases in fungicide experiments. Plant Dis. Reptr., 27, 340–343.
Turhan, O. (2010). Çilekte siyah kök çürüklüğü (Rhizoctonia solani Kühn.) hastalığına karşı bazı biyolojik kontrol ajanlarının etkileri. Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı Yüksek Lisans Tez, 59 (in Turkish).
Wilson, P.S., Ahvenniemi, P.M., Lehtonen, M.J., Kukkoren, M., Rita, H., Valkonen, J.P.T. (2008). Biological and chemical control and their combined use to control different stages of the Rhizoctonia disease complex on potato through the growing season. Ann. Appl. Biol., 153, 281–416. Wensing, A., Braun, S.D., Buttner, P., Expert, D., Volksch, B., Ullrich, M.S., Weingart, H. (2010). Impact of siderophore production by Pseudomonas syringae pv. syringae 22d/93 on epiphytic fitness and biocontrol activity against Pseudomonas syringae pv. glycinea 1a/96. Appl. Environ. Microbiol., 76, 2704–2711.
Adams, P.B. (1990). The potential of mycoparasites for biological control of plant diseases. Annu. Rev. Phytopathol., 28,59–72.
Anitha, A., Das, M.A. (2011). Activation of rice plant growth against Rhizoctonia solani using Pseudomonas fluorescens, Trichoderma and salicylicacid. Res. Biotechnol., 2, 7–12.
Aydin, M.H., Turhan, G., Gore, M.E. (2011). The effect of some antagonists on Rhizoctonia solani in potato tubers. Anadolu J. Agric. Sci., 21(2), 29–38. Braun, S.D., Hofmann, J., Wensing, A., Weingart, H., Ullrich, M.S., Spiteller, D., Volksch, B. (2010). In vitro antibiosis by Pseudomonas syringae Pss22d, acting against the bacterial blight pathogen of soybean plants, does not influence in planta biocontrol. J. Phytopathol., 158, 288–295.
Bautista, G., Mendoza, H., Urıbe, D. (2007). Biocontrol of Rhizoctonia solani in native potato (Solanum phureja) plants using native Pseudomonas fluorescens. Acta Biol. Colomb., 12(1),19–32.
Chernin, L., Ismailov, Z., Haran, S., Chet, I., 1995. Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl. Environ. Microbiol., 61, 1720–1726.
Cubukcu, N. (2007). Pamuklarda Verticillium solgunluğu (Verticillium dahliae Kleb.)’na karşı endofitik bakterilerle biyolojik mücadele olanakları. Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, 73 (in Turkish).
Demirer Durak, E. (2011). Erzurum ilinde çilek bitkilerinden izole edilen Rhizoctonia türlerinin anastomosis grupları, patojeniteleri ve biyolojik mücadeleleri. Atatürk Üniv. Fen Bilimleri En-stitüsü Doktora Tezi., 162 (in Turkish).
Demirci, E., Döken, M.T. (1993). Anastomosis groups and pathogenicity of Rhizoctonia solani Kühn isolates from potatoes in Erzurum-Türkiye. J. Turk. Phytopath., 22(2–3), 95–102.
Eken, C., Demirci, E. (2004). Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia isolates from bean in Erzurum, Turkey. J. Plant Pathol., 86(1), 49–52.
Errampalli, D., Johnston, W. (2001). Control of tuber-borne black scurf (Rhizoctonia solani) and common scab (Streptomyces scabies) of potato with a combination of sodium hypochloride and thiophanate-methyl preplanting seed tuber treatment. Can. J. Plant Pathol., 23, 68–77.
Groth, D.E., Bond, J.A. (2006). Initiation of rice sheath blight epidemics and effects of applica-tion timing of azoxystrobin on disease incidence, severity, yield and milling yields. Plant Dis., 90, 1073–1078.
Huang, X., Zhang, N., Yong, X., Yang, X., Shen, Q. (2011). Biocontrol of Rhizoctonia solani damping-off disease in cucumber with Bacillus pumilus SQR-N43. Microbiol. Res, 167, 135–143.
Jehtonen, M.J. (2009). Rhizoctonia solani as a potato pathogen variation of isolates in Finland and host response. University of Helsinki Finland, Academic Dissertation in Plant Pathology, p. 81.
Kilicoglu, M.C., Ozkoc, I. (2010). Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region. Turk. J. Biol., 34, 261–269.
Kilic-Ekici, O., Yuen, G.Y. (2003). Induced resistance as a mechanism of biological control by Lysobacter enzymogenes strain C3. Phytopathology, 93(9), 1103–1110.
Kotan, R., Sahin, F., Demirci, E., Eken, C. (2009). Biological control of the potato dry rot caused by Fusarium species using PGPR strains. Biol. Cont., 50, 194–198.
Liu, C.H., Chen, X., Liu, T.T., Lian, B., Gu, Y., Caer, V., Xue, Y.R., Wang, B.T. (2007). Study of the antifungal activity of Acinetobacter baumannii LCH001 in vitro and identification of its antifungalcomponents. Appl. Microbiol. Biotechnol., 76(2), 459–466.
Naeimi, S., Okhovvat, S.M., Javan-Nikkhah, M., Vagvolgyi, C., Khosravi, V., Kredics, L. (2010). Biological control of Rhizoctonia solani AG1-1A, the causal agent of rice sheath blight with Trichoderma strains. Phytopathol. Mediterr., 49, 287–300.
Ongena, M., Duby, F., Rossignol, F., Fouconnier, M.L., Dommes, J., Thonart, P. (2004). Stimula-tion of the lipoxygenase pathway is associated with systemic resistance induced in bean by a nonpathogenic Pseudomonas strain. Mol. Plant. Microbe Interact., 17,1009–1018.
Porras, M., Barrau, C., Romero, F. (2007). Effects of soil solarization and Trichoderma on straw-berry production. Crop Protect., 26, 782–787.
Ryu, C.M., Farag, M.A., Hu, C.H., Reddy, M.S., Kloepper, J.W., Pare, P.W. (2004). Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol., 134, 1017–1026.
Scherwinski, K., Grosch, R., Berg, G. (2008). Effect of bacterial antagonists on lettuce: active biocontrol of Rhizoctonia solani and negligible, short-termeffects on nontarget microorgan-isms. FEMS Microbiol. Ecol., 64 (1),106–116.
Tariq, M., Yasmin, S., Hafeez, F.Y. (2010). Biological control of potato black scurf by rhizo-sphere associated bacteria. Braz. J. Microbiol., 41, 439–451.
Tian, X.L., Cao, L.X., Tan, H.M., Zeng, Q.G., Jia, Y.Y., Han, W.Q., Zhou, S.N. (2003). Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their anti-pathogenic activities in vitro. World J. Microbiol. Biotechnol., 20, 303–309.
Townsend, G.K., Heuberger, J.W. (1943). Methods for estimating losses caused by diseases in fungicide experiments. Plant Dis. Reptr., 27, 340–343.
Turhan, O. (2010). Çilekte siyah kök çürüklüğü (Rhizoctonia solani Kühn.) hastalığına karşı bazı biyolojik kontrol ajanlarının etkileri. Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı Yüksek Lisans Tez, 59 (in Turkish).
Wilson, P.S., Ahvenniemi, P.M., Lehtonen, M.J., Kukkoren, M., Rita, H., Valkonen, J.P.T. (2008). Biological and chemical control and their combined use to control different stages of the Rhizoctonia disease complex on potato through the growing season. Ann. Appl. Biol., 153, 281–416. Wensing, A., Braun, S.D., Buttner, P., Expert, D., Volksch, B., Ullrich, M.S., Weingart, H. (2010). Impact of siderophore production by Pseudomonas syringae pv. syringae 22d/93 on epiphytic fitness and biocontrol activity against Pseudomonas syringae pv. glycinea 1a/96. Appl. Environ. Microbiol., 76, 2704–2711.
Mesude Figen Donmez
Igdir University Igdir, Turkey
Igdir University Igdir, Turkey
Badel Uysal
Igdir University Igdir, Turkey
Igdir University Igdir, Turkey
Erkol Demırcı
Karadeniz Technical University, Maçka Vocational School, Trabzon, Turkey
Karadeniz Technical University, Maçka Vocational School, Trabzon, Turkey
Sezai Ercisli
Atatürk University, Erzurum, Turkey
Atatürk University, Erzurum, Turkey
Ramazan Cakmakcı
Atatürk University, Erzurum, Turkey
Atatürk University, Erzurum, Turkey
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