Cytomolecular assay of size nucleolar organizer regions (NORs) polymorphism in Pietrain pigs
Barbara Danielak-Czech
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówBarbara Rejduch
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówMarek Babicz
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 LublinAbstract
The aim of this study was to identify and classify the size variants of nucleolar organizing regions (NORs) as markers useful for genetic characteristics of Pietrain breed pigs. On the
basis of cytomolecular analysis carried out by silver staining (Ag-I) and fluorescence in situ hybridization (FISH) techniques, four size variants of rDNA-FISH-signals and Ag-NORs silver
deposits were classified. Results of cytomolecular assay of size NORs polymorphism can be used in inter-breed or inter-species comparative surveys and studies of evolutionary relationships in Suidae family.
Keywords:
pigs, chromosomes, nucleolar organizing regions (NORs), genetic markers, FISH techniqueReferences
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Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
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