In vitro efficacy of herbal plant extracts on some phytopathogenic fungi

Weronika Kursa

Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0003-3206-4335

Agmieszka Jamiołkowska

Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0003-4121-5318

Barbara Skwaryło-Bednarz

Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0001-8608-9138

Radosław Kowalski

Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0003-3063-8710

Jakub Wyrostek

Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0002-0504-2862

Elżbieta Patkowska

Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0002-0742-352X

Marek Kopacki

Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland
https://orcid.org/0000-0001-7162-250X


Abstract

Crops are subject to yield losses caused by the presence of pests whose species and population diversity are changing with climate warming. The use of agrochemicals is still the most effective method of plant protection against diseases and pests. However, the intensive use of pesticides in some EU countries makes it necessary to search for alternative methods that can be applied in plant protection for consumer and environmental safety. The aim of the study was the laboratory evaluation of the fungistatic effect of extracts of herbal plants occurring in natural state in Poland: horseradish (Armoracia rusticana L.), yarrow (Achillea millefolium L.) and tansy (Tanacetum vulgare L.) on selected polyphagic phytopathogenic fungi (Alternaria alternata, Botrytis cinerea, Colletotrichum coccodes and Fusarium oxysporum). In this study, 5%, 10% and 20% concentrations of herbal water-extract extracts were applied to evaluate their effects on linear growth of fungi and inhibition of their growth relative to control. Total content of polyphenols and flavonoids was assessed in the extracts using spectrophotometry, and their antioxidant activity by applying the synthetic DPPH radical. The plant extracts of tansy and yarrow leaves were characterized by a higher content of polyphenols and flavonoids compared to horseradish leaf extracts; they also had a higher antioxidant activity. Plant extracts inhibited the growth of fungi to a different extent, depending on the species of fungus, type of extract, its concentration and duration of action. The strongest fungistatic effect was recorded for tansy and yarrow extracts, while the weakest for the extract of horseradish leaves. Plant extracts showed the weakest effect against Botrytis cinerea, inhibiting the development of this fungus only during the first days of the experiment. The present research is a preliminary study that will be used in the further to develop a biological preparation for the protection of agricultural and horticultural plants against fungal pathogens.

Keywords:

plant extracts, Armoracia rusticana, Achillea millefolium, Tanacetum vulgare, phytopathogenic fungi, flavonoids, polyphenols, DPPH

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2022-12-30



Weronika Kursa 
Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0003-3206-4335
Agmieszka Jamiołkowska 
Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0003-4121-5318
Barbara Skwaryło-Bednarz 
Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0001-8608-9138
Radosław Kowalski 
Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0003-3063-8710
Jakub Wyrostek 
Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0002-0504-2862
Elżbieta Patkowska 
Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0002-0742-352X
Marek Kopacki 
Department of Plant Protection, University of Life Sciences in Lublin, Lublin, Poland https://orcid.org/0000-0001-7162-250X



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