Size polymorphism survey of nucleolar organizer regions (NORs) in Hampshire boars
BARBARA DANIELAK-CZECH
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówMAREK BABICZ
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 LublinANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówBARBARA REJDUCH
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówAbstract
Previous studies revealed that precisely quantified and normalized NORs size variants may be considered as chromosome markers helpful in pig breeds characterization and gene mapping. The aim of the present study was to identify and classify the NORs size variants as markers useful for genetic characteristics of Hampshire pigs. On the basis of cytogenetic and molecular analysis (carried out by Ag-I and FISH techniques) four size variants of FISH-signals and AgNORs deposits were classified. Nucleolar organizer regions were more morphologically distinct and greater on both chromosomes of pair 10 than on pair 8, which suggests a dominant role of chromosome 10 in the global production of ribosomal RNA. Results of cytomolecular survey of NORs size polymorphism can be used for inter-breed or inter-species comparative assays and phylogenetic studies in Suidae family.
Keywords:
Hampshire boars, nucleolar organizing regions (NOR), size variants, chromosome markers, FISH technique, Ag-I techniqueReferences
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Wachtler F., Hopman A.H.N., Wiegant J., Schwarzacher H.G., 1986. On the position of nucleolus organizer regions (NORs) in interphase nuclei. Studies with a new, non-Autoradiographic In Situ Hybridization Method. Exp. Cell Res. 167, 227–240.
Żurkowski M., Kurył J., Różycki M., Kamyczek M., Janik A., Duniec M., Korwin-Kossakowska A., Niemczewski C., Czerwiński S., Buczyński J.T., 1995. The Polish Pig Genome Project: I. Characterization of the genetic structure of resource breeds and F1 generation on the basis of genetic markers. Anim. Sci. Pap. Rep.13 (2/3), 105–114.
Danielak-Czech B., Kaczor U., Sharan M., 2009. Chromosome markers survey of Duroc pigs. Ukrainian Academy of Agrarian Sciences. Anim. Biol. 11, 237–241.
Danielak-Czech B., Słota E., Babicz M., Kozubska-Sobocińska A., Rejduch B., 2006. Ocena wielkości regionów jąderkotwórczych (NOR) u świń rasy puławskiej. Rocz. Nauk. Zoot. 33, 13–19.
Danielak-Czech B., Słota E., Lindblad K., Gustavsson I., 1999. Size polymorphism of nucleolar organizer regions in pigs bred in Poland as determined by FISH and silver staining technique. Anim. Sci. Pap. Rep. 17, 163–171.
Danielak-Czech B., Rejduch B., Babicz M., 2011. Cytomolecular assay of size nucleolar organizer regions (NORs) polymorphism in Pietrain pigs. Annales UMCS, sect. EE, Zootechnica, 29(4), 33–38.
Harding R.M., Boyce A.J., Clegg J.B., 1992. The evolution of tandemly repetitive DNA: Recombination rules. Genetics 132, 847–859.
Komisarek J., Szydłowski M., Świtoński M., Kurył J., 1998a. The use of chromosomal markers for the identification of QTLs controlling fattening, and carcass and meat quality traits in pigs. Proc. 13th Eur. Colloq. Cytogenet. Domest. Anim., 51.
Komisarek J., Świtoński M., Pietrzak A., Klukowska A., 1998b. The Polish Pig Genome Mapping Project: IV. Segregation of chromosomal markers in generation F2. Anim. Sci. Pap. Rep. 16 (2), 77–81.
Liu W.S., Lu X.Z., Qiu H., 1995. Number and distribution of silver stained nucleolar organizer regions and evolutionary relationships in domestic pigs. Anim. Genet. 26, 293–298.
Lomholt B., Christenssen K., Hallenberg C., Frederiksen J., 1995. Porcine 5S rRNA genes mapped to 14q23 revealing syntenic relation to human HSPA6- and 7. Mamm. Genome 6, 439–441.
Mellink C.H.M., Bosma A.A., De Haan N.A., Wiegant J., 1991. Distribution of rRNA genes in breeds of domestic pig studied by non-radioactive in situ hybridization and selective silverstaining. Genet. Sel. Evol. 23 (suppl. 1), 168–172.
Mellink C.H.M., Bosma A.A., De Haan N.A., 1994. Variation in size of Ag-NORs and fluorescent rDNA in situ hybridization signals in six breeds of domestic pig. Hereditas 120 (2), 141–149.
Mellink C.H.M., Bosma A.A., De Haan N.A., Zijlstra C., 1996. Physical localization of 5S rRNA genes in the pig by fluorescence in situ hybridization. Hereditas 124, 95–97.
Miyake M.L., O’Brien S.J., Kaneda Y., 1988. Regional localization of rDNA genes on pig chromosome 10 by in situ hybridization. Jap. J. Vet. Sci. 50, 341–345.
Pinkel D., Straume T., Gray J.W., 1986. Cytogenetic analysis using quantitative, high sensitive, fluorescence hybridization. Proc. Nat. Acad. Sci. USA 83, 2934.
Słota E., 1998. Polymorphism of pig chromosomes. Rocz. Nauk. Zoot. (Habilitation Thesis), 1–59.
Solinas Toldo S., Pieńkowska A., Fries R., Świtoński M., 1992. Localization of nucleolar organizer regions in farm animals by in situ hybridization method with a probe from a human rRNA gene. Proc. 10th Eur. Colloq. Cytogenet. Domest. Anim., 228–231.
Świtoński M., Pietrzak A., 1992. Cytogenetic survey of AI boars; distribution of C-band and AgNORs polymorphism. Anim. Sci. Pap. Rep. 9, 91–96.
Świtoński M., Komisarek J., Pietrzak A., 1997a. The Polish Pig Genome Project: III. Chromosomal markers in generation F1. Anim. Sci. Pap. Rep. 15 (2), 93–99.
Świtoński M., Pietrzak A., Buczyński J., 1997b. Chromosomal markers (C-band and Ag-NOR) in the Zlotnicka Spotted pig. Anim. Sci. Pap. Rep. 15 (3), 173–178.
Wachtler F., Hopman A.H.N., Wiegant J., Schwarzacher H.G., 1986. On the position of nucleolus organizer regions (NORs) in interphase nuclei. Studies with a new, non-Autoradiographic In Situ Hybridization Method. Exp. Cell Res. 167, 227–240.
Żurkowski M., Kurył J., Różycki M., Kamyczek M., Janik A., Duniec M., Korwin-Kossakowska A., Niemczewski C., Czerwiński S., Buczyński J.T., 1995. The Polish Pig Genome Project: I. Characterization of the genetic structure of resource breeds and F1 generation on the basis of genetic markers. Anim. Sci. Pap. Rep.13 (2/3), 105–114.
BARBARA DANIELAK-CZECH
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
MAREK BABICZ
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
ANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
BARBARA REJDUCH
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
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