Cross-species hybridizations in situ of genes associated with meat production traits in the wild pig genome
ANNA KOZUBSKA-SOBOCIŃSKA
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówBARBARA DANIELAK-CZECH
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/KrakówMAREK BABICZ
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 LublinAbstract
Ghrelin (GHRL) and uncoupling proteins UCP2, UCP3 play a functional role in global energy metabolism in the body, growth and obesity as well as meat organoleptic quality. The aim of this study was to analyze homology between the regions of human chromosomes involving the encoding loci GHRL, UCP2, UCP3 and the corresponding fragments in the wild pig (Sus scrofa scrofa) genome using the FISH technique. Two commercial human probes (Vysis), specific for regions of 3. and 11, pair autosomes (HSA3p25-26 and HSA11q13) were used for crosshybridizations in situ with wild pig chromosomes – karyotype 36,XY,rob(15;17). The following physical locations were established – the GHRL gene was identified in wild pig autosomal interstitial region SSC13q31-32 whereas UCP2 and UCP3 genes, due to their proximity, were mapped to the same chromosome region SSC9p21-24. Cross-species in situ hybridizations confirmed conservation of the linkage groups and a high degree of homology of chromosome regions containing GHRL, UCP2 and UCP3 loci in human and the domestic and wild pig genomes.
Keywords:
wild pig, comparative FISH mapping, cross-species hybridizations in situ, GHRL, UCP2, UCP3 genesReferences
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Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Departament of Animal Cytogenetics and Molecular Genetics, National Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków
Department of Pig Breeding and Production Technology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin
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